Abstract:
:Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labeled at 1 h after injection. Between 1 and 24 h after injection there was a very marked decrease in the concentration of label in the pancreatic acini. Radioautography at the ultrastructural level demonstrated that the earliest time interval the silver grains indicating the sites of newly synthesized glycoproteins were predominantly observed over the Golgi apparatus of the acinar cells. The quantitative analysis indicated that condensing vacuoles were labeled from 10 min to 4 h after injection. At 1 h the concentration of label in the Golgi apparatus was greatly diminished while it was significantly higher in the secretory (zymogen) granules. At this interval the secretion product visualized in the lumen of the acini and of the ducts was found to be labeled. At 4 h both the zymogen granules and the plasma membrane were significantly labeled. Further analysis carried out on the zymogen granules indicated that fucose-labeled glycoproteins were located in the granule membrane as well as in the granule content. In conclusion, 3H-fucose is added to glycoproteins in the Golgi apparatus of the acinar cells. Then, labeled glycoproteins are collected within condensing vacuoles and zymogen granules and released into the acinar lumen as secretion products. This latter event, which almost certainly is a consequence of exocytosis, may also account for the addition of granule-membrane glycoproteins to the plasma membrane.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Haddad A,Brasileiro ILdoi
10.1007/BF00222243subject
Has Abstractpub_date
1983-01-01 00:00:00pages
197-207issue
1eissn
0302-766Xissn
1432-0878journal_volume
233pub_type
杂志文章abstract::To investigate the uptake and transport patterns of variously sized particles in Peyer's patches (PPs) of calves, intestinal loops were created in four newborn and two 2-month-old calves, and the loops were inoculated with various particles, including carbon black, fluorescein isothiocyanate (FITC)-labeled latex, FITC...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0793-y
更新日期:2009-07-01 00:00:00
abstract::The retina of the phalangid, Opilio ravennae, consists of retinula cells with distal rhabdomeres, arhabdomeric cells, and sheath cells. The receptive segment of retinula cells shows a clear separation into a proximal rhabdom, organized into distinct rhabdom units formed by three or four retinula cells, and a distal rh...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233658
更新日期:1979-01-01 00:00:00
abstract::Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 microM....
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0903-x
更新日期:2010-02-01 00:00:00
abstract::It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218485
更新日期:1982-01-01 00:00:00
abstract::Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223227
更新日期:1975-01-01 00:00:00
abstract::Angiogenesis, the growth of new blood vessels, is required for a variety of normal proliferative processes. Furthermore, angiogenesis is well established as also playing an important role in neoplastic growth and metastasis. Numerous regulators of angiogenesis have been identified and characterized over the last few d...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-008-0675-8
更新日期:2009-01-01 00:00:00
abstract::The pinealocytes of the pig contain conspicuous dense bodies, the nature and role of which are not yet fully elucidated. The aim of this study was to demonstrate whether or not these structures are involved in the secretion process. The tannic acid-Ringer incubation (TARI)-method, which allows a clear-cut ultrastructu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313977
更新日期:1991-05-01 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::Interstitial cells in the pineal gland of the rat were characterized immunocytochemically using the monoclonal antibodies MRC OX-42 and ED1 for macrophages/microglia, and MRC OX-6, which recognizes major histocompatibility complex (MHC) class II antigen. A polyclonal antibody against GFAP was used to identify astrocyt...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00302731
更新日期:1993-05-01 00:00:00
abstract::We previously described the presence of MHC class II (HLA-DR) antigens, structurally similar to those on lymphoid cells and bearing the genetically-appropriate allotypic determinants, on human adrenocortical cells in the zona reticularis of normal glands. We now report a similar expression by granulosa-lutein cells (G...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00309876
更新日期:1990-11-01 00:00:00
abstract::The ultrastructural localization of ryanodine receptors (RyR) in sinus endothelial cells of the rat spleen was examined by confocal laser scanning and electron microscopy by using isoform-specific antibodies to each of the RyR isoforms. Immunofluorescence microscopy of tissue cryosections revealed RyR3 to be localized...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0904-8
更新日期:2004-08-01 00:00:00
abstract::Calcitonin gene-related peptide-immunoreactive cells were identified within the epithelium of distal conducting airways in the human fetus and infant. Several peptides and amines, including calcitonin, have been identified previously within a specific population of airway epithelial cells. These cells, referred to as ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218949
更新日期:1987-12-01 00:00:00
abstract::The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a -RFamide C-terminus and are structurally similar to sulfakinin peptides; in contrast, DSK 0 conta...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050518
更新日期:1996-01-01 00:00:00
abstract::During the first postnatal weeks of the developing rodent retina, rhodopsin can be detected in a number of neuron-like cells in the inner retina. In the present study, we aim to characterize the morphology, number and staining characteristics of this peculiar population. Misplaced rhodopsin-positive cells (MRCs) were ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1788-2
更新日期:2014-04-01 00:00:00
abstract::The comparatively simple Caenorhabditis elegans intestine fulfills many of the complex functions of the mammalian digestive tract, liver, and fat tissues, while also having roles in pathogen defense, immunity, and longevity. In this review, we describe the structure of the C. elegans gut and how it develops from the e...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03036-4
更新日期:2019-09-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-010-1049-6
更新日期:2011-01-01 00:00:00
abstract::Tenidap is a structurally novel antirheumatic agent with anti-inflammatory and analgesic properties. Previous studies have shown that tenidap is able to inhibit the production and action of cytokines such as interleukin-1, interleukin-6 (IL-6) and tumour necrosis factor alpha. However, the mechanisms by which tenidap ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050810
更新日期:1997-05-01 00:00:00
abstract::The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222230
更新日期:1983-01-01 00:00:00
abstract::Guanosine triphosphatase (GTPase) activity was studied histo- and cytochemically in the rod outer segments of the rat retina by means of a newly developed method. Differences in the distribution pattern of the enzyme activity exist within the outer segment: the activity is more intense at the tip of the rod outer segm...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217416
更新日期:1983-01-01 00:00:00
abstract::The pineal organ of the blind, cave-dwelling fish, Typhlichythyes subterraneous, was examined with both light and electron microscopes. Like the eyes, the pineal in this troglobytic species was found to be regressed. Two cell types, photoreceptor and supportive cells, were described in the pineal epithelium. Although ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233895
更新日期:1978-12-29 00:00:00
abstract::Electron microscopy of the rat lymph node disclosed reticular cells in close association with bundles of collagenous fibrils 30-45 nm in diameter, and electron lucent "amorphous" substances surrounded by microfibrils 11 nm in diameter, with junctional complexes. The amorphous substances, distinctly revealed by orcein ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210522
更新日期:1981-01-01 00:00:00
abstract::Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-007-0480-9
更新日期:2008-01-01 00:00:00
abstract::Terrestrial hermit crabs of the genus Coenobita display strong behavioral responses to volatile odors and are attracted by chemical cues of various potential food sources. Several aspects of their sense of aerial olfaction have been explored in recent years including behavioral aspects and structure of their periphera...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03199-5
更新日期:2020-06-01 00:00:00
abstract::The ultrastructure of the contractile apparatus of the rat soleus muscle during the course of denervation atrophy was investigated. It was found that the ratio of thin the thick filaments increased in myofibrils of atrophying muscle fibers. Elevation of the ratio was observed as early as the second day after denervati...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216767
更新日期:1981-01-01 00:00:00
abstract::Ex-fissiparous planarians produce infertile cocoons or, in very rare cases, cocoons with very low fertility. Here, we describe the features of programmed cell death (PCD) occurring in the hyperplasic ovary of the ex-fissiparous freshwater planarian Dugesia arabica that may explain this infertility. Based on TEM result...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1971-0
更新日期:2014-11-01 00:00:00
abstract::More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217555
更新日期:1985-01-01 00:00:00
abstract::Using anti-carp-gonadotropic-gamma-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224328
更新日期:1976-03-16 00:00:00
abstract::The luminal surface features and junctional complexes from developing blood vessels in the rat central nervous system have been studied by high-voltage electron microscopy and scanning electron microscopy. Developing blood vessels exhibit three types of luminal projections; marginal folds or ridges at junctional compl...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222417
更新日期:1977-01-04 00:00:00
abstract::Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiot...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0636-2
更新日期:2008-08-01 00:00:00
abstract::The azygos vein of the rat can be divided into three regions: The proximal cardiac region, where the wall is composed of two and sometimes three layers of cardiac muscle and a thin discontinuous layer of smooth muscle cells. Vascular casts of this region demonstrate layers of capillaries closely following the orientat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221867
更新日期:1986-01-01 00:00:00