Abstract：
:The extracellular matrix (ECM) acts as a critical factor during morphogenesis. Because the organization of the ECM directly influences the structure of tissues and organs, a determination of the way that ECM organization is regulated should help to clarify morphogenesis. We have analyzed the assembly of Del1, an ECM protein produced by endothelial cells in embryos, in the ECM. Del1 consists of three epidermal growth factor repeats (E1-E3) at its N-terminus and two discoidin domains (C1, C2) at its C-terminus. Experiments with various deletion mutants of Del1 have revealed that fragments containing the C-terminus of C1, which has a lectin-like structure, direct deposition in the ECM. The efficiency of deposition varies according to the presence of other domains in Del1. A fragment containing E3 and C1 has the strongest deposition activity, whereas fragments containing C2, which is highly homologous to C1, have low deposition activity. Digestion of ECM with hyaluronidase from bovine testis releases Del1 from the ECM, suggesting that glycosaminoglycans are involved in the deposition of Del1. In vivo gene transfer experiments have shown that fusion with the deposition domain of Del1 dramatically alters the distribution of exogenous proteins in mice. Thus, the extent of Del1 deposition may modify the organization of the ECM.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Hidai C,Kawana M,Kitano H,Kokubun Sdoi
10.1007/s00441-007-0456-9subject
Has Abstractpub_date
2007-10-01 00:00:00pages
83-95issue
1eissn
0302-766Xissn
1432-0878journal_volume
330pub_type
杂志文章abstract：:The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...
journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217304
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220968
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：2013-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209978
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1657-z
更新日期：2013-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216577
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233562
更新日期：1979-03-09 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2418-6
更新日期：2016-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00300227
更新日期：1994-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100380
更新日期：2001-06-01 00:00:00
abstract：:Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318364
更新日期：1995-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1988-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2000-05-01 00:00:00
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更新日期：2012-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2001-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0701-x
更新日期：2008-12-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1997-05-01 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/BF00207693
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/BF00222157
更新日期：1976-10-29 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225416
更新日期：1985-01-01 00:00:00
