Abstract:
:Germ cells are set aside from somatic cells early in embryogenesis, and are responsible for transmitting genetic information through generations. Vasa is a highly conserved germ cell marker across animal phyla, and widely used to label primordial germ cells. Dabry's sturgeon is a rare and endangered species distributed solely in the Yangtze River basin. Here, seven vasa isoforms, named Advasa1-7, were isolated and characterized in Dabry's sturgeon. RT-PCR and western blot analyses revealed that vasa mRNA and protein were mainly restricted to the testis and ovary, but exhibited sexually dimorphic expression. Cellular and subcellular localization uncovered that Advasa mRNA and protein displayed mitotic and meiotic expression in females, and mainly showed mitotic expression in males; surprisingly, they exhibited both cytoplasmic and nuclear expression in the ovarian germ cells, while showing exclusively cytoplasmic expression in the testicular germ cells. By microinjecting chimeric RNA consisting of the red fluorescent protein coding region and the Advasa 3'-untranslated region into embryos of Dabry's sturgeon, zebrafish and medaka, we demonstrated that it had the ability to visualize primordial germ cells (PGCs) in Dabry's sturgeon and zebrafish but not in medaka. It seemed that the machinery of vasa 3'UTR RNA localization was conserved between Dabry's sturgeon and ostariophysan, while possibly changed during the divergence of euteleosts and ostariophysan. Finally, Dabry's sturgeon PGCs moved on the yolk ball, and migrated toward the genital ridge via mesenchyme. Taken together, these results provide new information for vasa expression pattern and function, and lay a foundation for PGC cryopreservation and conservation of Dabry's sturgeon.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ye H,Yue HM,Yang XG,Li CJ,Wei QWdoi
10.1007/s00441-016-2418-6subject
Has Abstractpub_date
2016-10-01 00:00:00pages
203-18issue
1eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-016-2418-6journal_volume
366pub_type
杂志文章abstract::Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats,...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051119
更新日期:1998-08-01 00:00:00
abstract::The vascular architecture of the rat testis and spermatic cord was studied by a corrosion cast technique combined with scanning and transmission electron microscopy, and light microscopy. The casts preserve the endothelial impressions of the vessels and enable them to be differentiated into the various vascular compon...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217176
更新日期:1985-01-01 00:00:00
abstract::In three mature monkeys (Macaca fascicularis; 3.5 +/- 0.3 kg in weight), the labial, buccal and soft-palate mucosae were examined morphologically and stereologically. Using fixation by perfusion, standardized methods of tissue preparation and morphometric analysis at the light-microscopic level, the gross dimensions (...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213740
更新日期:1984-01-01 00:00:00
abstract::Previous studies have identified Dogiel type II neurons with cell bodies in the myenteric plexus of guinea-pig ileum to be intrinsic primary afferent neurons. These neurons also have distinctive electrophysiological characteristics (they are AH neurons) and 82-84% are immunoreactive for calbindin. They are the only ca...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100395
更新日期:2001-07-01 00:00:00
abstract::Double-labeling immunofluorescent histochemistry demonstrates that calretinin, a calcium-binding protein, coexists with calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in the fibers innervating the lamina propria of the rat intestinal villi. An acetylcholinesterase histochemical stain r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318366
更新日期:1995-06-01 00:00:00
abstract::The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224663
更新日期:1976-08-26 00:00:00
abstract::Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0668-7
更新日期:2008-10-01 00:00:00
abstract::Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222069
更新日期:1975-01-01 00:00:00
abstract::The effects of high dose injections of corticotropin-releasing hormone (CRH) on the adrenal cortex of hypophysectomized rats were studied at the light- and electron-microscopical levels. Adrenocortical atrophy induced by hypophysectomy could be reduced by daily i.p. injection of 10 micrograms (3 nmol) CRH given for 3 ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00297501
更新日期:1990-04-01 00:00:00
abstract::Colonies of the ascidian Botryllus schlosseri undergo recurrent generation changes in which adult zooids are gradually resorbed and replaced by new blastogenic generations. During these periods, known as take-over phases, programmed cell death, which, on the basis of morphological analysis is ascribed to apoptosis, oc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-003-0738-9
更新日期:2003-06-01 00:00:00
abstract::The fine structure of synapses, their distribution and arrangement in the ventral nerve cord of Peripatoides leuckarti (phylum Onychophora) is described. The asymmetric synaptic junctions show a well developed synaptic cleft (300 A) and pre- and subsynaptic electron dense apposition. They frequently show an array of p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224940
更新日期:1978-01-31 00:00:00
abstract::The prothoracicotropic hormone (PTTH), which stimulates ecdysteroid synthesis in the prothoracic glands, is produced, in the dorso-lateral protocerebrum of Manduca sexta, by paired peptidergic neurons, the lateral neurosecretory cell group III (L-NSC III). Our study revealed ultrastructural features of L-NSC III, iden...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00303082
更新日期:1994-07-01 00:00:00
abstract::Accumulating evidence suggests that the sympathetic nervous system modulates inflammatory responses and bone remodeling. We have studied the effects of sympathectomy and orthodontic tooth movement (OTM) on root resorption, immunocompetent cell recruitment, neuropeptide, neurokinin-1 receptor (NK1-R), and interleukin 6...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-003-0753-x
更新日期:2003-08-01 00:00:00
abstract::The development of the rat adrenal medulla was studied at the ultrastructural level with particular emphasis placed on early discrimination of different catecholamine-storing cells. The first granule-containing cells, phaeochromoblasts, were seen at day 15 of gestation migrating into the anlage of the cortex. These ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233834
更新日期:1981-01-01 00:00:00
abstract::We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215891
更新日期:1986-01-01 00:00:00
abstract::Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234830
更新日期:1979-04-30 00:00:00
abstract::We have previously shown that the chromogranin A (CgA)-derived peptide catestatin (CST: hCgA352-372) inhibits nicotine-induced secretion of catecholamines from the adrenal medulla and chromaffin cells. In the present study, we seek to determine whether CST regulates dense core (DC) vesicle (DCV) quanta (catecholamine ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2956-1
更新日期:2019-04-01 00:00:00
abstract::Dexamethasone (Dex) regulates osteoblastic and adipocytic differentiation in mesenchymal progenitor cells through regulation of Wnt/β-catenin signaling. To elucidate the regulatory mechanisms underlying the effects of Dex, we examine the expression of Axin2, which is an intracellular inhibitor of Wnt/β-catenin signali...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1696-5
更新日期:2013-12-01 00:00:00
abstract::Isotocinergic (IT) neurons show physiological and morphological characteristcs that are similar to those of the other preoptic neuroendocrine cells in the goldfish. Preoptic IT cells show resting membrane potentials of 20-55 mV, action potentials of up to 100 mV, and physiological evidence of axonal branching. Dye-mar...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00239217
更新日期:1979-10-02 00:00:00
abstract::The intrapancreatic localization and the effects on basal and stimulated insulin secretion of neuropeptide Y (NPY) were investigated in the mouse. Immunocytochemistry showed NPY to be confined to intrapancreatic nerve fibers mainly associated with blood vessels. Fine varicose NPY fibers were also detected in the exocr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF01239960
更新日期:1987-04-01 00:00:00
abstract::Mice and rats emit and perceive calls in the ultrasonic range, i.e., above the human hearing threshold of about 20 kHz: so-called ultrasonic vocalizations (USV). Juvenile and adult rats emit 22-kHz USV in aversive situations, such as predator exposure and fighting or during drug withdrawal, whereas 50-kHz USV occur in...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1607-9
更新日期:2013-10-01 00:00:00
abstract::The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis vi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0744-z
更新日期:2009-03-01 00:00:00
abstract::The protonephridial flame cell of the freshwater planarian Bdellocephala brunnea was studied by electron microscopy. This basketlike cell with luminal cilia and fenestrae is known as a cyrtocyte. Careful examination revealed the actual continuity of the fenestrae over the surface of the cell and the microvillous natur...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237973
更新日期:1980-01-01 00:00:00
abstract::Rat endometrial explants were cultured in a three-dimensional collagen/endothelial cell matrix to measure angiogenic activity, as represented by migration of vascular endothelial cells towards the explants. Minimal endothelial cell migratory activity was observed with endometrial explants taken during the four-day oes...
journal_title:Cell and tissue research
pub_type: 历史文章,杂志文章
doi:10.1007/BF00300706
更新日期:1995-01-01 00:00:00
abstract::In the rostral hypothalamus of the domestic fowl, the magnocellular neurosecretory nuclei show a peculiar differentiation. Golgi studies of the supraoptic and paraventricular nuclei of the fowl reveal at least two major cell types: 1) large multipolar neurons, and 2) small interneurons. Golgi impregnations provide a d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236253
更新日期:1981-01-01 00:00:00
abstract::The vertebrate limb is a powerful model system for studying the cellular and molecular interactions that determine morphological pattern during embryonic development. Recent advances in our understanding of these interactions have shed new light on the molecular mechanisms of vertebrate limb development, evolution and...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410051261
更新日期:1999-04-01 00:00:00
abstract::In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabecu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236349
更新日期:1979-01-30 00:00:00
abstract::Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (P...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1446-0
更新日期:2012-10-01 00:00:00
abstract::The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-001-0499-2
更新日期:2002-02-01 00:00:00
abstract::Comparing the distribution of cytoplasmic particles and organelles between different experimental conditions can be challenging due to the heterogeneous nature of cell morphologies. The border-to-border distribution method was created to enable the quantitative analysis of fluorescently labeled cytoplasmic particles a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2265-x
更新日期:2016-02-01 00:00:00