Catestatin regulates vesicular quanta through modulation of cholinergic and peptidergic (PACAPergic) stimulation in PC12 cells.

Abstract:

:We have previously shown that the chromogranin A (CgA)-derived peptide catestatin (CST: hCgA352-372) inhibits nicotine-induced secretion of catecholamines from the adrenal medulla and chromaffin cells. In the present study, we seek to determine whether CST regulates dense core (DC) vesicle (DCV) quanta (catecholamine and chromogranin/secretogranin proteins) during acute (0.5-h treatment) or chronic (24-h treatment) cholinergic (nicotine) or peptidergic (PACAP, pituitary adenylyl cyclase activating polypeptide) stimulation of PC12 cells. In acute experiments, we found that both nicotine (60 μM) and PACAP (0.1 μM) decreased intracellular norepinephrine (NE) content and increased 3H-NE secretion, with both effects markedly inhibited by co-treatment with CST (2 μM). In chronic experiments, we found that nicotine and PACAP both reduced DCV and DC diameters and that this effect was likewise prevented by CST. Nicotine or CST alone increased expression of CgA protein and together elicited an additional increase in CgA protein, implying that nicotine and CST utilize separate signaling pathways to activate CgA expression. In contrast, PACAP increased expression of CgB and SgII proteins, with a further potentiation by CST. CST augmented the expression of tyrosine hydroxylase (TH) but did not increase intracellular NE levels, presumably due to its inability to cause post-translational activation of TH through serine phosphorylation. Co-treatment of CST with nicotine or PACAP increased quantal size, plausibly due to increased synthesis of CgA, CgB and SgII by CST. We conclude that CST regulates DCV quanta by acutely inhibiting catecholamine secretion and chronically increasing expression of CgA after nicotinic stimulation and CgB and SgII after PACAPergic stimulation.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Sahu BS,Mahata S,Bandyopadhyay K,Mahata M,Avolio E,Pasqua T,Sahu C,Bandyopadhyay GK,Bartolomucci A,Webster NJG,Van Den Bogaart G,Fischer-Colbrie R,Corti A,Eiden LE,Mahata SK

doi

10.1007/s00441-018-2956-1

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

51-70

issue

1

eissn

0302-766X

issn

1432-0878

pii

10.1007/s00441-018-2956-1

journal_volume

376

pub_type

杂志文章
  • Morphological and functional changes in the tight junctions of the bile canaliculi induced by bile duct ligation.

    abstract::Thin sections after bile duct ligation showed that the depth of tight junctions appeared to increase and that the distance between individual punctate contacts appeared to become irregular and wider than in controls. The freeze fracture replicas clearly demonstrated these changes in the tight junction morphology. Chan...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00236724

    authors: Koga A,Todo S

    更新日期:1978-12-28 00:00:00

  • Role of notochord cells and sclerotome-derived cells in vertebral column development in fugu, Takifugu rubripes: histological and gene expression analyses.

    abstract::Despite the common structure of vertebrates, the development of the vertebral column differs widely between teleosts and tetrapods in several respects, including the ossification of the centrum and the function of the notochord. In contrast to tetrapods, vertebral development in teleosts is not fully understood, parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-016-2404-z

    authors: Kaneko T,Freeha K,Wu X,Mogi M,Uji S,Yokoi H,Suzuki T

    更新日期:2016-10-01 00:00:00

  • Fine structure of the horny teeth of the lamprey, Entosphenus japonicus.

    abstract::The fine structure of the horny teeth of the lamprey, Entosphenus japonicus, was examined by light- and electron-microscopy. Most of the horny teeth consisted of two horny and two nonhorny layers. The primary horny layer was well keratinized, and the cells were closely packed and intensely interdigitated, being joined...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215769

    authors: Uehara K,Miyoshi S,Toh H

    更新日期:1983-01-01 00:00:00

  • Tinnitus: animal models and findings in humans.

    abstract::Chronic tinnitus (ringing of the ears) is a medically untreatable condition that reduces quality of life for millions of individuals worldwide. Most cases are associated with hearing loss that may be detected by the audiogram or by more sensitive measures. Converging evidence from animal models and studies of human ti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-014-1992-8

    authors: Eggermont JJ,Roberts LE

    更新日期:2015-07-01 00:00:00

  • Human and rodent bone marrow mesenchymal stem cells that express primitive stem cell markers can be directly enriched by using the CD49a molecule.

    abstract::Bone marrow (BM) from human and rodent species contains a population of multipotential cells referred to as mesenchymal stem cells (MSCs). Currently, MSCs are isolated indirectly by using a culture step and then the generation of fibroblast colony-forming units (CFU-fs). Unprocessed or native BM MSCs have not yet been...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0292-3

    authors: Gindraux F,Selmani Z,Obert L,Davani S,Tiberghien P,Hervé P,Deschaseaux F

    更新日期:2007-03-01 00:00:00

  • Differential expression of type 2 and type 3 inositol 1,4,5-trisphosphate receptor mRNAs in various mouse tissues: in situ hybridization study.

    abstract::The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP3R1) is predominantly expressed in cereb...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00307790

    authors: Fujino I,Yamada N,Miyawaki A,Hasegawa M,Furuichi T,Mikoshiba K

    更新日期:1995-05-01 00:00:00

  • Fine structure of seminiferous tubules from prenatally irradiated rats.

    abstract::The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminifer...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218294

    authors: Abreu I,David-Ferreira JF

    更新日期:1982-01-01 00:00:00

  • A note on the structure of synapses in the ventral nerve cord of the onychophoran Peripatoides leuckarti.

    abstract::The fine structure of synapses, their distribution and arrangement in the ventral nerve cord of Peripatoides leuckarti (phylum Onychophora) is described. The asymmetric synaptic junctions show a well developed synaptic cleft (300 A) and pre- and subsynaptic electron dense apposition. They frequently show an array of p...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00224940

    authors: Schürmann FW

    更新日期:1978-01-31 00:00:00

  • Immunofluorescent localization of sites binding anti-synthetic LHRH serum in the median eminence of the greenfinch (Chloris chloris L.).

    abstract::With an indirect immunofluorescence technique and an antiserum raised against synthetic LHRH, immunofluorescent granules were observed in fine radially oriented fibres in the palisade layer of the zoma externa in both the anterior and the posterior divisions of the median eminence in the greenfinch. There was no speci...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00223264

    authors: Sharp PJ,Haase E,Fraser HM

    更新日期:1975-09-16 00:00:00

  • Shaping oral cell plasticity to osteogenic differentiation by human mesenchymal stem cell coculture.

    abstract::In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-013-1777-5

    authors: Proksch S,Steinberg T,Vach K,Hellwig E,Tomakidi P

    更新日期:2014-04-01 00:00:00

  • An electron-microscopic study of the bone-remodeling sequence in the rat.

    abstract::A detailed chronological electron-microscopic study of the bone remodeling sequence has been performed in the rat based on a previously described model (Tran Van et al. 1982) in which the remodeling activity is synchronized. This allowed the observation of the cellular and extracellular events during the bone remodeli...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214682

    authors: Tran Van P,Vignery A,Baron R

    更新日期:1982-01-01 00:00:00

  • The ultrastructure of an olfactory sensillum on the maxillary palps of locusta migratoria (L.).

    abstract::The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219899

    authors: Blaney WM

    更新日期:1977-11-07 00:00:00

  • Complex relationships between the pineal organ and the medial habenular nucleus-pretectal region of the mouse as revealed by S-antigen immunocytochemistry.

    abstract::S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00313528

    authors: Korf HW,Sato T,Oksche A

    更新日期:1990-09-01 00:00:00

  • Access and distribution of exogenous substances in the intercellular clefts of the rat adenohypophysis.

    abstract::In model experiments with the use of horseradish peroxidase (HRP), two pathways of transport of substances to the adenohypophysis were studied, as well as the distribution of the tracer in the latter organ. The first pathway allows the tracer to penetrate from the intercellular milieu of the median eminence below the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214248

    authors: Krisch B,Buchheim W

    更新日期:1984-01-01 00:00:00

  • Binding sites of fluorescent derivatives of insulin in nuclei, rough endoplasmic reticulum, and mitochondria.

    abstract::Intracellular insulin-binding sites were directly traced in fixed monolayer cultures of a variety of cell types with the use of two fluorescent derivatives of insulin, viz. fluorescein isothiocyanate (FITC)-labelled and tetramethyl rhodamine isothiocyanate (TMRITC)-labelled insulin. Both derivatives retained the prope...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00232150

    authors: Lolait SJ,Toh BH

    更新日期:1980-01-01 00:00:00

  • An electron-microscope and freeze-fracture study of the egg cortex of Brachydanio rerio.

    abstract::We have examined the cortex of the teleost (Brachydanio rerio) egg before and during exocytosis of cortical granules by scanning, transmission, and freeze-fracture electron microscopy. In the unactivated egg, the P-face of the plasma membrane exhibits a random distribution of intramembranous particles, showing a densi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00398079

    authors: Hart NH,Collins GC

    更新日期:1991-08-01 00:00:00

  • Spatial and temporal regulation of glycosylation during Drosophila eye development.

    abstract::Glycosylation plays an essential role during development, in processes such as morphogen distribution, cell-to-cell communication, and extracellular matrix formation. Glycosylation is regulated during development in both a spatial and temporal manner. This study presents a detailed description of glycan distribution f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0753-6

    authors: Yano H,Yamamoto-Hino M,Goto S

    更新日期:2009-04-01 00:00:00

  • Effects of 1,25- and 24,25-dihydroxyvitamin D3 on bone formation in the cichlid teleost Sarotherodon mossambicus.

    abstract::1,25-Dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 have antagonistic effects on the acellular bone of the tilapia Sarotherodon mossambicus. 1,25-Dihydroxyvitamin D3 inhibits the activity of the lining osteoblasts. Prolonged administration leads to demineralization of the bone matrix. Injection of 24,25-dihydroxyvi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00206270

    authors: Wendelaar Bonga SE,Lammers PI,van der Meij JC

    更新日期:1983-01-01 00:00:00

  • Evidence for neural progenitor cells in the human adult enteric nervous system.

    abstract::Putative neural stem cells have been identified within the enteric nervous system (ENS) of adult rodents and cultured from human myenteric plexus. We conducted studies to identify neural stem cells or progenitor cells within the submucosa of adult human ENS. Jejunum tissue was removed from adult human subjects undergo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-011-1130-9

    authors: Azan G,Low WC,Wendelschafer-Crabb G,Ikramuddin S,Kennedy WR

    更新日期:2011-05-01 00:00:00

  • The major ganglion in the pelvic plexus of the male rat: a histochemical and ultrastructural study.

    abstract::To further evaluate the role of autonomic ganglia in the regulation of pelvic visceral activity, the neural elements in the major pelvic ganglion of the male rat have been studied with histochemixal and electron microscopic techniques. The principal findings are that the ganglion is composed of cholinergic and adrener...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00231994

    authors: Dail WG,Evan AP,Eason HR

    更新日期:1975-05-27 00:00:00

  • The role of Treg subtypes in glomerulonephritis.

    abstract::While Th1 and Th17 T effector cells are pathogenic drivers of glomerulonephritis (GN), regulatory T cells (Tregs) potently protect from renal tissue injury. Recently, it has become evident that different Treg subtypes exist. Among these are lineage specific Treg1 and Treg17 cells, which are specialized to down regulat...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-020-03359-7

    authors: Herrnstadt GR,Steinmetz OM

    更新日期:2020-12-14 00:00:00

  • Ultrastructural and morphometric study of the lung of the European salamander, Salamandra salamandra L.

    abstract::Ultrastructural and morphometric investigations were performed on the lung of the European salamander, Salamandra salamandra L. Folds of first and second order are covered with a ciliated epithelium containing goblet cells. The respiratory surface of the lung is lined by a single type of cell which, in amphibians, com...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222429

    authors: Goniakowska-Witalińska L

    更新日期:1978-07-27 00:00:00

  • In-advance trans-medullary stimulation of bone marrow enhances spontaneous repair of full-thickness articular cartilage defects in rabbits.

    abstract::Mesenchymal stromal cells (MSCs), especially those lying close to cartilage defects, are an important cell source for cartilage regeneration. We hypothesize that a larger number of MSCs might become available, if the bone marrow in the immediate vicinity of the subchondral bone is stimulated for MSCs in advance of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-1020-6

    authors: Nishizawa K,Imai S,Mimura T,Kubo M,Araki S,Shioji S,Takemura Y,Matsusue Y

    更新日期:2010-09-01 00:00:00

  • Effects of light and dark on photoreceptors in the polychaete annelid Nereis limnicola.

    abstract::The effects of light and dark on photoreceptors of the brackish-water polychaete annelid Nereis limnicola were studied by electron microscopy. Animals dark-adapted for one or two days exhibited well-formed straight microvilli (rhabdomeres) on the sensory cell processes. Continuous illumination of worms for one or two ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00225427

    authors: Eakin RM,Brandenburger JL

    更新日期:1985-01-01 00:00:00

  • Long-term staining of live Merkel cells with FM dyes.

    abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-002-0688-7

    authors: Fukuda J,Ishimine H,Masaki Y

    更新日期:2003-03-01 00:00:00

  • Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris.

    abstract::The distribution of FMRFamide-like immunoreactive cell bodies and fibers in the nervous system of the earthworm Lumbricus terrestris has been studied by means of immunocytochemistry. The cerebral ganglion contains 150-170 immunoreactive nerve cells that are organized into six major groups in the rostral and five major...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050843

    authors: Reglödi D,Slezák S,Lubics A,Szelier M,Elekes K,Lengvári I

    更新日期:1997-06-01 00:00:00

  • Electron microscopic study of the regeneration in vitro of rat peritoneal mast cells after histamine secretion.

    abstract::Regeneration of rat mast cells was studied by TEM from 10 s to 48 h after secretion of histamine induced by compound 48/80. During the first 2 h, small intracellular cavities, formed during compound exocytosis and containing non-membrane-bound remnants of the granules, tended to coalesce, and after 2 h of incubation r...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00238658

    authors: Nielsen EH,Bytzer P,Clausen J,Chakravarty N

    更新日期:1981-01-01 00:00:00

  • The retina of the phalangid, Opilio ravennae, with particular reference to arhabdomeric cells.

    abstract::The retina of the phalangid, Opilio ravennae, consists of retinula cells with distal rhabdomeres, arhabdomeric cells, and sheath cells. The receptive segment of retinula cells shows a clear separation into a proximal rhabdom, organized into distinct rhabdom units formed by three or four retinula cells, and a distal rh...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233658

    authors: Schliwa M

    更新日期:1979-01-01 00:00:00

  • Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events.

    abstract::Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-017-2726-5

    authors: Sebrell TA,Sidar B,Bruns R,Wilkinson RA,Wiedenheft B,Taylor PJ,Perrino BA,Samuelson LC,Wilking JN,Bimczok D

    更新日期:2018-02-01 00:00:00

  • Broad immunocytochemical localization of the formylpeptide receptor in human organs, tissues, and cells.

    abstract::The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptid...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051042

    authors: Becker EL,Forouhar FA,Grunnet ML,Boulay F,Tardif M,Bormann BJ,Sodja D,Ye RD,Woska JR Jr,Murphy PM

    更新日期:1998-04-01 00:00:00