Abstract:
:Mesenchymal stromal cells (MSCs), especially those lying close to cartilage defects, are an important cell source for cartilage regeneration. We hypothesize that a larger number of MSCs might become available, if the bone marrow in the immediate vicinity of the subchondral bone is stimulated for MSCs in advance of the creation of cartilage defects. A trans-medullary passage-way reaching the immediate vicinity of the subchondral bone was created 4 days prior to the creation of cartilage defects. In another setting, basic fibroblast growth factor (bFGF) was administered through the trans-medullary passage-way in order to augment the stimulation of MSCs. The rabbits were killed at various times after the creation of cartilage defects. Triple staining of bromodeoxyuridine (BrdU), CD44 and CD45 and histological evaluation were subsequently performed. A considerable proportion of the proliferating cells were identified as bone-marrow-derived MSCs. Enumeration of BrdU-positive cells demonstrated that trans-medullary stimulation, especially with bFGF, increased the number of proliferating cells. The histological grading score of trans-medullary stimulation with bFGF group was superior to that of the other groups. Thus, in-advance stimulation of the bone marrow effectively increases the number of proliferating cells. The putative progenitor cells for chondrocytes stimulated thereby are likely to be recruited to the osteochondral defects at the appropriate time, contributing to the repair of full-thickness articular cartilage defects at the early follow-up time point.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Nishizawa K,Imai S,Mimura T,Kubo M,Araki S,Shioji S,Takemura Y,Matsusue Ydoi
10.1007/s00441-010-1020-6subject
Has Abstractpub_date
2010-09-01 00:00:00pages
371-9issue
3eissn
0302-766Xissn
1432-0878journal_volume
341pub_type
杂志文章abstract::Rhabdomeres of the Drosophila melanogaster canoemisl mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900107
更新日期:1999-12-01 00:00:00
abstract::The interendothelial junctions of all segments of the renal vasculature have been studied in eight species using the freeze-fracture technique. Three types of junctions have been found. Combinations of tight and gap junction elements are characteristic for interlobular arteries and proximal afferent arterioles. Contin...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217224
更新日期:1984-01-01 00:00:00
abstract::To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232246
更新日期:1979-11-01 00:00:00
abstract::Hypothalamic slices containing the lateral hypothalamic area (LHA) were prepared from 6- to 8-day-old rats and maintained in stationary culture for up to 35 days in order to analyse how well the melanin-concentrating hormone (MCH) neurons survived. As previously reported for other brain areas, this method yielded a lo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051330
更新日期:1999-07-01 00:00:00
abstract::An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone ahd no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216742
更新日期:1981-01-01 00:00:00
abstract::Guanosine triphosphatase (GTPase) activity was studied histo- and cytochemically in the rod outer segments of the rat retina by means of a newly developed method. Differences in the distribution pattern of the enzyme activity exist within the outer segment: the activity is more intense at the tip of the rod outer segm...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217416
更新日期:1983-01-01 00:00:00
abstract::The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219899
更新日期:1977-11-07 00:00:00
abstract::Sipunculans, a small phylum of coelomated marine worms closely related to polychaete annelids, lack a true circulatory system. We have previously shown that the sipunculan Themiste petricola can form a cellular clot, without congealing, of cell-free coelomic fluid. The clot is formed by the aggregation of large granul...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0912-9
更新日期:2010-03-01 00:00:00
abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238295
更新日期:1983-01-01 00:00:00
abstract::The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233914
更新日期:1979-03-19 00:00:00
abstract::The pars tuberalis of the hypophysis of the Djungarian hamster, Phodopus sungorus, was investigated with regard to secretory activity by applying the tannic acid-Ringer perfusion technique. Two groups were maintained under long photoperiods (16 h light: 8 h dark) or short photoperiods (8 h light: 16 h dark), respectiv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00312830
更新日期:1993-08-01 00:00:00
abstract::PRDM1 (PR domain containing 1) is a transcriptional repressor that has been identified in various species and is crucial for cell growth, differentiation and development. However, the expression pattern and role of PRDM1 in development has not been sufficiently established in birds. We therefore investigate the spatio...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1804-1
更新日期:2014-05-01 00:00:00
abstract::This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other musc...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-005-0019-x
更新日期:2005-11-01 00:00:00
abstract::During tooth development, the inner and outer enamel epithelia fuse by mitotic activity to produce a bilayered epithelial sheath termed Hertwig's epithelial root sheath (HERS). The epithelial rests of Malassez (ERM) are the developmental residues of HERS and remain in the adult periodontal ligament (PDL). Although the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-1076-3
更新日期:2010-12-01 00:00:00
abstract::The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were compa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209040
更新日期:1978-10-17 00:00:00
abstract::The fine structure of synapses, their distribution and arrangement in the ventral nerve cord of Peripatoides leuckarti (phylum Onychophora) is described. The asymmetric synaptic junctions show a well developed synaptic cleft (300 A) and pre- and subsynaptic electron dense apposition. They frequently show an array of p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224940
更新日期:1978-01-31 00:00:00
abstract::The octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. This system is organized similarly in all larval anurans and in all adult aglossids. All have two groups of efferent neurons in the nucleus reticularis medialis between the VIIIth and th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214812
更新日期:1982-01-01 00:00:00
abstract::In the intestine of the filtering zooids of B. schlosseri three segments can be distinguished. In the intermediate segment the epithelium, which is encrusted by the ampullae of the pyloric gland, shows marked aspects of alteration. In the proximal and distal segments, ciliated mucous, vacuolated and endocrine-like cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219771
更新日期:1977-08-26 00:00:00
abstract::SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1618-6
更新日期:2013-06-01 00:00:00
abstract::Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217166
更新日期:1985-01-01 00:00:00
abstract::The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2127-6
更新日期:2015-04-01 00:00:00
abstract::Cestodes (tapeworms) are a derived, parasitic clade of the phylum Platyhelminthes (flatworms). The cestode body wall represents an adaptation to its endoparasitic lifestyle. The epidermis forms a non-ciliated syncytium, and both muscular and nervous system are reduced. Morphological differences between cestodes and fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0687-8
更新日期:2003-03-01 00:00:00
abstract::In vitro incubation of autonomic nerves with vinblastine sulphate (1 times 10(-4) M) caused a disappearance of microtubules within 15 min; during the following 15 min paracrystalline arrays appeared within the axons. An increase in the abundance of microfilaments was also observed, but these did not appear to arise fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220136
更新日期:1976-02-25 00:00:00
abstract::This study documents neuronal differentiation in vitro from undifferentiated precursor cells of caudalmost regenerating spinal cord of the teleost Apteronotus albifrons. At 11 days in vitro, cells from the caudalmost tip of the regenerating cord are flat and polygonal in shape, lack neuronal processes and do not stain...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00414166
更新日期:1994-11-01 00:00:00
abstract::The smooth muscle of rabbit portal vein was studied by electron microscopy with particular emphasis on the mechanical linkage between the muscle cells and on the distribution of connective tissue. The media of this vein is composed of inner circular and outer longitudinal muscle layers which are orientated almost perp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237614
更新日期:1980-01-01 00:00:00
abstract::Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2453-3
更新日期:2016-11-01 00:00:00
abstract::The extracellular matrix (ECM) acts as a critical factor during morphogenesis. Because the organization of the ECM directly influences the structure of tissues and organs, a determination of the way that ECM organization is regulated should help to clarify morphogenesis. We have analyzed the assembly of Del1, an ECM p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0456-9
更新日期:2007-10-01 00:00:00
abstract::The electrocyte fibers in the gymnotid Sternarchus albifrons are highly differentiated myelinated axons which exhibit several types of nodes of Ranvier and characteristically short internode lengths. In the present study, regeneration of the electrocyte fibers following removal of the tail was examined by electron mic...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233869
更新日期:1980-01-01 00:00:00
abstract::The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chrom...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218655
更新日期:1983-01-01 00:00:00
abstract::The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051160
更新日期:1998-10-01 00:00:00