Abstract:
:The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were comparatively estimated for the different components of mitochondria-rich fibres, in particular of red fibres, from the tibialis anterior muscle and of mitochondria-poor fibres from the oesophageal muscle. As early as 2 min after injection, radioactivity was detected over the nucleus, the polysome-rich sarcoplasm, the A and I bands, the Z lines, and the mitochondria in the two fibre types. Label localization did not change with time. The relative label concentration increased similarly in the polysome-rich sarcoplasm and the A and I bands of both fibre types within 30 min after injection, a confirmation that biosynthesis of myofibrillar proteins takes place rapidly. In each case, concentration was higher in the Z lines than in the I bands, and higher in the I bands than in the A bands, thus showing "in vivo" that the rates of synthesis of sarcomere protein components are not uniform. However, the relative label concentration was found to be higher in the Z lines of mitochondria-poor fibres than of mitochondria-rich fibres: this suggests that a higher synthetic rate of Z line protein, and probably of alpha actinin, is characteristic of the first type. Inversely, the concentration was higher in the mitochondria of mitochondria-rich fibres. This lead to the belief that high rate of protein synthesis in these organelles may account for the high rate of label incorporation into this type of fibre.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Dadoune JP,Terquem A,Alfonsi MFdoi
10.1007/BF00209040subject
Has Abstractpub_date
1978-10-17 00:00:00pages
269-82issue
2eissn
0302-766Xissn
1432-0878journal_volume
193pub_type
杂志文章abstract::The ultrastructure of the seminiferous tubules was studied in rats that had been subjected to whole body irradiation on the 19th day of gestation. The seminiferous tubules from 3 months-old irradiated animals are devoid of germ cells and contain only Sertoli cells. Compared with controls of the same age, the seminifer...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218294
更新日期:1982-01-01 00:00:00
abstract::17beta-estradiol (E2) is important for crustacean ovarian development. This study aims to investigate the distribution and change pattern of E2 in the ovary, hepatopancreas, thoracic ganglion and brain ganglion as well as Vg-mRNA expression level during ovarian development of Chinese mitten crab Eriocheir sinensis. Re...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2834-x
更新日期:2018-08-01 00:00:00
abstract::Previous studies have indicated the importance of basement membrane components both for cellular differentiation in general and for the barrier properties of cerebral microvascular endothelial cells in particular. Therefore, we have examined the expression of basement membrane proteins in primary capillary endothelial...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0604-1
更新日期:2002-10-01 00:00:00
abstract::Seasonal changes have been detected in the ultrastructure of brown adipose tissue (BAT) of the common shrew, Sorex araneus (a true nonhibernator) living under natural conditions and collected at the time when the most representative growth phase of the animal for the given season could be expected. In summer and autum...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233501
更新日期:1981-01-01 00:00:00
abstract::The central fat body of the ovoviviparous cockroach Nauphoeta cinerea was studied during the first reproductive cycle of the female by means of light microscopy, autoradiography and electron microscopy. Comparative studies in larval stages were also undertaken. The fat body of Nauphoeta contains a large amount of lipi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219785
更新日期:1978-04-28 00:00:00
abstract::The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233613
更新日期:1980-01-01 00:00:00
abstract::Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410051072
更新日期:1998-06-01 00:00:00
abstract::The endothelial layer of blood vessels controls the passage of cells and solutes from the blood into the surrounding tissue. Crucial for this regulation is the integrity of endothelial cell-cell junctions. Various molecular mechanisms control junctional integrity of the endothelial layer including GTPases, modulation ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-1812-1
更新日期:2014-03-01 00:00:00
abstract::Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209276
更新日期:1978-05-29 00:00:00
abstract::1,25-Dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 have antagonistic effects on the acellular bone of the tilapia Sarotherodon mossambicus. 1,25-Dihydroxyvitamin D3 inhibits the activity of the lining osteoblasts. Prolonged administration leads to demineralization of the bone matrix. Injection of 24,25-dihydroxyvi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00206270
更新日期:1983-01-01 00:00:00
abstract::Bone resorption by mononucleated cells was studied in the acellular bone of a teleost fish (Oreochromis niloticus) by histological and enzyme histochemical observations and by transmission electron microscopy. Bone resorbing cells (osteoclasts) were identified by their location at the sites of bone resorption, their f...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050782
更新日期:1997-02-01 00:00:00
abstract::Radiofrequency lesions were made in the lateral geniculate nuclei of six squirrel monkeys. The resulting degenerating terminals and their postsynaptic structures in layer IV of area 17 were quantitatively categorized on photomontages covering large areas of neuropil. Two to five days after the lesion, numerous axon te...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234741
更新日期:1979-02-28 00:00:00
abstract::In adrenalectomized rats the influence of salt loading or salt deprivation on the vasopressin and oxytocin content of the median eminence (ME) and the neural lobe (NL) was studied by means of various methods: morphometric and microphotometric analysis of aldehyde fuchsin-stained sections of ME and NL; immunohistochemi...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/BF00215904
更新日期:1986-01-01 00:00:00
abstract::The effects of cytochalasin B (CB) on pancreatic structure and amylase release were studied by use of pancreatic fragments, isolated acini and isolated acinar cells. In pancreatic fragments and isolated acini CB caused the disappearance of microfilaments underlying the apical plasma membrane, loss of apical microvilli...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219848
更新日期:1977-04-29 00:00:00
abstract::This study provides information on the rates of DNA synthesis, sites of DNA synthesis, and DNA content of the avian salt gland during the osmotic stressing (plasma membrane synthesis) and destressing (plasma membrane turnover) cycle, in an effort to better understand the relationship of cell turnover to the initial ev...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214782
更新日期:1982-01-01 00:00:00
abstract::This report demonstrates that the administration of testosterone (T) or 11-ketotestosterone (11-KT) to sexually immature (8 wks old) male platyfish (Xiphophorus maculatus) of early- and late-maturing genotypes affects the synthesis and/or release of luteinizing hormone-releasing hormone (LHRH), as assessed by immunocy...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218552
更新日期:1986-01-01 00:00:00
abstract::Endothelins (ET) are a family of vasoactive peptides that act via two subtypes of receptors, named ETA and ETB. ET-1 binds to both ETA and ETB, whereas the isopeptide ET-3 preferentially binds to ETB. The localization of ETA and ETB receptors in the rat adrenal gland and their involvement in the adrenal secretagogue e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050820
更新日期:1997-05-01 00:00:00
abstract::The presence of immunoreactive enkephalin, dynorphin, vasoactive intestinal polypeptide, cholecystokinin, substance P and neuropeptide Y in nerve fibers that project to the guinea-pig inferior mesenteric ganglion was analysed, after different denervation and ligation procedures. A quantitative analysis demonstrates th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219741
更新日期:1988-09-01 00:00:00
abstract::Numerous macrophages were found aggregated in the lamina propria at the tips of villi in the small intestine of guinea pigs. These macrophages extended their pseudopodia into the epithelial lining and internalized fragments of effete enterocytes in their phagosomes. The epithelium of the villus tips was found to be in...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF02913723
更新日期:1993-03-01 00:00:00
abstract::The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1889-6
更新日期:2014-09-01 00:00:00
abstract::Electron microscopy of the pineal receptor cells in light- and dark-adapted brook trout, Salvelinus fontinalis and the rainbow trout, Salmo gairdneri, revealed no significant differences in the tubular and filamentous elements of the inner segment, neck and supranuclear regions. However, changes in synaptic relations ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234177
更新日期:1980-01-01 00:00:00
abstract::The three-dimensional structure of the mitochondria and sarcoplasmic reticulum (SR) in the three types of twitch fibers, i.e., the red, white and intermediate skeletal muscle fibers, of the vastus lateralis muscle of the Japanese meadow frog (Rana nigromaculata nigromaculata Hallowell) was examined by high resolution ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218939
更新日期:1987-12-01 00:00:00
abstract::Taste buds and the peripheral nerves innervating them are two important components of the peripheral gustatory system. They require appropriate connections for the taste system to function. Neurotrophic factors play crucial roles in the innervation of peripheral sensory organs and tissues. Both brain-derived neurotrop...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0833-7
更新日期:2009-09-01 00:00:00
abstract::Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234830
更新日期:1979-04-30 00:00:00
abstract::Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214555
更新日期:1985-01-01 00:00:00
abstract::Rhabdomeres of the Drosophila melanogaster canoemisl mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900107
更新日期:1999-12-01 00:00:00
abstract::With the gallocyanine technique (Wittkowski, Bock and Franken, 1970) Gomori-positive substances of the infundibulum can be stained for light- and electron-microscopic examination. In various mammalian species, the size of Gomori-positive elementary granules in the outer layer is markedly different from that in the inn...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218495
更新日期:1975-11-19 00:00:00
abstract::Exocytotic release of the secretory granules of the endocrine cells in the midgut of a cockroach, Periplaneta americana, was studied by means of fixation with tannic acid in combination with glutaraldehyde and osmium tetroxide. A sequence of images indicative of exocytosis suggests the following steps in this process:...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213851
更新日期:1982-01-01 00:00:00
abstract::The intrapancreatic localization and the effects on basal and stimulated insulin secretion of neuropeptide Y (NPY) were investigated in the mouse. Immunocytochemistry showed NPY to be confined to intrapancreatic nerve fibers mainly associated with blood vessels. Fine varicose NPY fibers were also detected in the exocr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF01239960
更新日期:1987-04-01 00:00:00
abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1701-z
更新日期:2013-12-01 00:00:00