Histo- and cytochemical demonstration of guanosine triphosphatase activity in the outer segments of rods in the rat retina by means of a newly developed method.

Abstract:

:Guanosine triphosphatase (GTPase) activity was studied histo- and cytochemically in the rod outer segments of the rat retina by means of a newly developed method. Differences in the distribution pattern of the enzyme activity exist within the outer segment: the activity is more intense at the tip of the rod outer segments near the pigment epithelium than in their proximal portion. Ultracytochemically, the new procedure reveals the reaction product of GTPase activity partly (i) on the extradisk membrane side and (ii) on the disk membranes. This result is in contrast to the cytochemical localization of guanylate cyclase (GCLase), an enzyme also localized at the tip of the rod outer segments: GCLase activity is restricted to the intradisk membrane area of the rod outer segments. The functional role of GTPase activity in the outer segments of rods is discussed.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Ueno S,Bambauer HJ,Umar H,Ueck M

doi

10.1007/BF00217416

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

225-30

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

234

pub_type

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