当前位置： SCI文献检索 > CELL AND TISSUE RESEARCH期刊下所有文献 > In-vitro formation of a new fibrous attachment to human dental roots in the presence of autologous serum.

In-vitro formation of a new fibrous attachment to human dental roots in the presence of autologous serum.

Abstract：

:Recent studies have accomplished the establishment of a collagenous fiber-fringe matrix upon dental root surfaces in vitro. The present study was undertaken to follow the development of such a matrix in vitro and to test the possible effects of root surface treatments upon this matrix. Periodontal ligament cells, 0.1 to 0.2-mm thick dental root discs, and alveolar bone cells were derived after extraction from four partially erupted third molars and the accompanying interradicular bony septa of 1 male patient. Autologous serum was obtained by venipuncture. Cultures were initiated by delivering a 1-ml suspension of 50,000 tritiated thymidine-labeled periodontal ligament cells and 50,000 alveolar bone cells onto each of 42 culture sets. The following day, demineralized or non-demineralized root discs treated with autologous serum, fibronectin or complete medium were placed in pairs, separated by a 0.1-1.0 mm gap, upon the initial cell layer. Representative cultures were terminated after 2, 3, 4, 5 and 6 weeks, and processed for light- and electron microscopy, morphometric analysis and autoradiography. An outstanding feature of the early cultures (2, 3 and 4 weeks) was a patchwise, random distribution of matrix making a precise developmental study impossible, although collagen fibrils were produced within the first 2 weeks. Some 3-week cultures already demonstrated a mature fiber-fringe characterized electron-microscopically as oriented, densely packed collagen fibrils closely abutting the cementum-lined root discs. The treatments (including autologous serum) used in this study had no appreciable morphologic or morphometric effect upon the fiber-fringe formed. Because none of the cultures in the present or past studies have demonstrated a true cementoid matrix, this model may not be suitable for the in-vitro study of cementum formation.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Bernstein AB,Preisig E,Pajarola G,Schroeder HE

doi

10.1007/BF00223152

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

125-35

issue

eissn

0302-766X

issn

1432-0878

journal_volume

258

pub_type

杂志文章

在线工具

In-vitro formation of a new fibrous attachment to human dental roots in the presence of autologous serum.