Abstract:
:Prolactin cells were identified by means of immunocytochemistry with protein-A gold as a marker on ultrathin sections of the pituitary gland of young (3-4 months), middle-aged (16-19 months), and aged (26-30 months) male Wistar rats. Point-counting volumetry revealed that the prolactin (PRL) cell-volume density in middle-aged rats was significantly increased in comparison to the volume densities in young and aged rats. Within the PRL-cell population, four types of PRL cells were distinguished on the basis of the shape and size of their secretory granules. During aging, dramatic changes occurred in the relative volumes of the four cell types. The volume percentage of cells with round granules (type I, granule diameter 150-250 nm, and type IIA, granule diameter 250-350 nm) increased from +/- 30% in young rats to +/- 90% in old rats. The volume percentage of cells with round and polymorphic granules (type IIB; granule diameter 350-400 nm and type III; granule diameter 500-600 nm) decreased from +/- 70% in young rats to +/- 7% in old rats. Age-related changes in serum PRL levels were not found. It is concluded that although during the life span of the male Wistar rat considerable changes in PRL-cell volume densities and in the ratios of PRL-cell types occur serum, PRL levels remain more or less constant.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
van Putten LJ,Kiliaan AJdoi
10.1007/BF00215844subject
Has Abstractpub_date
1988-02-01 00:00:00pages
353-8issue
2eissn
0302-766Xissn
1432-0878journal_volume
251pub_type
杂志文章abstract::Generally, the CRF-like activity of vasopressin is studied in experiments involving adrenalectomy and corticosteroid replacement. In order to avoid this complex type of stress, male and female (diestrus, estrus) rats were exposed to 5 min to immobilization stress and sacrificed 5, 15, and 30 min thereafter. After a su...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209276
更新日期:1978-05-29 00:00:00
abstract::Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050943
更新日期:1997-11-01 00:00:00
abstract::The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized R...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050575
更新日期:1996-04-01 00:00:00
abstract::A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0561-9
更新日期:2008-04-01 00:00:00
abstract::Methods for isolation and culture of microvascular endothelial cells of the inner ear were devised to provide an in-vitro system for studying endothelial functions in this tissue. Capillaries from the stria vascularis and spiral ligament were treated enzymatically to free them from surrounding tissue. Contamination by...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214566
更新日期:1985-01-01 00:00:00
abstract::The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219899
更新日期:1977-11-07 00:00:00
abstract::A somatostatin-like substance is demonstrated by light microscopic immunohistochemistry (PAP-method) in perikarya and cell processes of the retina of adult and infant rats. These perikarya are identified according to their size, arrangement and distribution. Each of the first two neuronal orders (receptors, bipolar ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00235169
更新日期:1979-01-01 00:00:00
abstract::S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313528
更新日期:1990-09-01 00:00:00
abstract::Nitric oxide (NO) stimulates osteoblast differentiation, but whether NO contributes to odontoblast differentiation during dentin repair is unknown. By using reverse transcription/polymerase chain reaction and immunostaining, we investigated the gene expression and/or immunolocalization of endothelial NO synthase (eNOS...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0003-5
更新日期:2007-04-01 00:00:00
abstract::Obesity induces metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases and has become a global health concern. Recent studies imply that fat accumulation in nonadipose tissue correlates with metabolic disorders. However, there are no suitable animal models to evaluate this phenomenon. T...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2908-9
更新日期:2019-02-01 00:00:00
abstract::Recently, an antibody against the choline transporter (CHT), an essential molecule involved in ACh uptake, was used to label cholinergic nerves in the central nervous system; however, the enteric nervous system (ENS) was not examined. The present study localised CHT immunoreactivity (CHT-IR) within the rat ileum ENS a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0332-z
更新日期:2007-03-01 00:00:00
abstract::The fan-shaped body is the largest substructure of the central complex in Drosophila melanogaster. Two groups of large-field neurons that innervate the fan-shaped body, viz., F1 and F5 neurons, have recently been found to be involved in visual pattern memory for "contour orientation" and "elevation" in a rut-dependent...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-009-0781-2
更新日期:2009-06-01 00:00:00
abstract::With the gallocyanine technique (Wittkowski, Bock and Franken, 1970) Gomori-positive substances of the infundibulum can be stained for light- and electron-microscopic examination. In various mammalian species, the size of Gomori-positive elementary granules in the outer layer is markedly different from that in the inn...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218495
更新日期:1975-11-19 00:00:00
abstract::SCO-spondin is a newly identified protein, strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When secreted into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, it condenses and forms Reissner's fiber. Several conserved domains have previously been charact...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051132
更新日期:1998-09-01 00:00:00
abstract::In previous studies the differentiation of the epithelium in the human hard palate has been described stereologically using parameters expressed per unit tissue volume. Since single epithelial cells represent the true biological units of this tissue, it became necessary to estimate the absolute size of such cells in o...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216577
更新日期:1981-01-01 00:00:00
abstract::This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other musc...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-005-0019-x
更新日期:2005-11-01 00:00:00
abstract::In the present study, immunogold labeling of ultrathin sections of rat small intestine and liver has been used to obtain insights into the ultrastructural localization and possible functions of annexins. In enterocytes, annexins II, IV, and VI are found at the periphery of the core of each microvillus and of the rootl...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00414181
更新日期:1994-11-01 00:00:00
abstract::We have examined radioautographically the protein synthetic and secretory activity of differentiating odontoblasts and ameloblasts, exposed for 9 h in vitro to various concentrations of colchicine in the presence of 3H-proline. Colchicine impairs the cytodifferentiation of the dental epithelium into ameloblasts and of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216651
更新日期:1988-06-01 00:00:00
abstract::Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214555
更新日期:1985-01-01 00:00:00
abstract::Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220036
更新日期:1988-10-01 00:00:00
abstract::Post-translational modification of histone proteins, such as the methylation of lysine and arginine residues, influences the higher order of chromatin and leads to gene activation or silencing. Histone methyltransferases or demethylases actively add or remove various methylation marks in a cell-type-specific and conte...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-1842-8
更新日期:2014-06-01 00:00:00
abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) and helospectin are two vasoactive intestinal polypeptide (VIP)-related neuropeptides that have recently been demonstrated in the mammalian gut; the aim of this study was to reveal their occurrence and localisation in the gastrointestinal tract, swimbladder, u...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00300227
更新日期:1994-09-01 00:00:00
abstract::LDH is localized along various intracytoplasmatic membranes of osteoclasts. Macrophages show membrane-bound LDH only after phagocytosis of calcium hydroxylapatite, the main mineral constituent of bone. The localization of LDH in these macrophages is almost the same as in osteoclasts. The significance of this finding, ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219927
更新日期:1980-01-01 00:00:00
abstract::Agitation of villi evokes reflexes that affect the motility of the guinea-pig small intestine. NK1 receptor endocytosis was used to investigate the possible involvement of tachykinins acting on neuronal NK1 receptors in these reflexes. Segments of guinea-pig ileum were incubated at 37 degrees C in Krebs physiological ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051032
更新日期:1998-04-01 00:00:00
abstract::Larvae of Ascidia malaca, both before and after hatching, were treated with L-thyroxine solutions. The effect of the thyroid hormone was to induce the onset of metamorphosis and then to cause the rate at which body reorganization occurred to increase. In treated larvae the resorption of the tail occurred only few hour...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00249213
更新日期:1981-01-01 00:00:00
abstract::Apoptosis is a form of programmed cell death that occurs in neurons during development of the nervous system and may also be a prominent form of neuronal death in chronic neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Recent findings also implicate apoptosis in neuronal degeneration after is...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004419900154
更新日期:2000-07-01 00:00:00
abstract::From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-012-1339-2
更新日期:2012-03-01 00:00:00
abstract::VIP-containing neurons were localized in lungs from dogs, cat, and human subjects by means of the indirect immunofluorescence technique. Nerve fibers and terminals were observed in the smooth muscle layer and glands of airways, and within the walls of pulmonary and bronchial vessels, especially at the medial-adventiti...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210505
更新日期:1981-01-01 00:00:00
abstract::Removal of the somatic DNA methylation pattern from donor cells and remodeling of embryonic status have been suggested as integral processes for successful nuclear transfer (NT) reprogramming. This study has investigated the effects of 5-azacytidine (5-azaC), a DNA methylation inhibitor, on global methylation changes ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0201-9
更新日期:2006-09-01 00:00:00
abstract::Administration of Concanavalin A (Con A) to cultured skin fibroblasts derived from chick embryos at two developmental stages produce variations in the relative concentration of individual glycosaminoglycan (GAG) secreted by the cells. This effect is different: at 7 days (increase of hyaluronic acid and dermatan sulpha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217295
更新日期:1984-01-01 00:00:00