Binding of antibody to the gamma subunit of FCepsilonRI in rat basophilic leukemia cells results in morphological changes without inducing histamine release.


:The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized RBL-2H3 cells and 125I goat anti-rabbit IgG. After purification of the serum on a protein G column, RBL-2H3 cells, mouse mast cells, and human basophils showed a doublet at 20 000 kDa. When 125I labeled, saponin-permeabilized cells were immunoprecipitated with the anti-gamma antibody, all 3 subunits of the IgE receptor complex coprecipitated. Furthermore, binding of the antibody to the cell surface did not induce histamine release. By immunofluorescence of fixed cells, the receptor was evenly distributed in the RBL-2H3 cells. Although no capping was observed when the cells were incubated with the antibody prior to fixation, the antibody did stimulate endocytosis when it was bound to the cells at 25 degrees C or at 37 degrees C, but not at 4 degrees C. Binding of the antibody to the RBL-2H3 cells also induced cell spreading and ruffling of the plasma membrane. The results of this study indicate that the gamma subunit of the high affinity IgE receptor may play a role in signal transduction.


Cell Tissue Res


Cell and tissue research


Jamur MC,Oliver C




Has Abstract


1996-04-01 00:00:00












  • The pattern of innervation of a polyneural muscle: axolotl iliotibialis.

    abstract::The pattern of innervation on individual iliotibialis muscle fibres from axolotl (Ambystoma mexicanum) has been investigated histologically and electrophysiologically. These polyneural fibres were found to be innervated on average at five end plate sites. The sites were distributed irregularly along each fibre. Averag...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Slack JR,Docherty JR

    更新日期:1978-01-09 00:00:00

  • CRF-containing neurons of the rat hypothalamus.

    abstract::The immunoreactive CRF-neurons of the rat hypothalamus have been examined immunohistochemically employing anti-rat CRF serum. These neurons are confined to the paraventricular nucleus, dorsomedial-lateral hypothalamic area, and suprachiasmatic nucleus, and are, respectively, also immunoreactive to anti-Met-enk, -alpha...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Daikoku S,Okamura Y,Kawano H,Tsuruo Y,Maegawa M,Shibasaki T

    更新日期:1985-01-01 00:00:00

  • Use of microarrays to find novel regulators of periodontal ligament fibroblast differentiation.

    abstract::Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell population...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lallier TE,Spencer A

    更新日期:2007-01-01 00:00:00

  • Human melanomas express functional P2 X(7) receptors.

    abstract::Adenosine 5'-triphosphate is known to function as a potent extracellular messenger, producing its effects via a distinct family of cell surface receptors. Different receptor subtypes have been shown to modulate different cellular functions such as proliferation, differentiation and apoptosis. We have investigated the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: White N,Butler PE,Burnstock G

    更新日期:2005-09-01 00:00:00

  • Some evidence for the ampullary organs in the European cave salamander Proteus anguinus (Urodela, Amphibia).

    abstract::The multicellular epithelial organs in Proteus anguinus, which Bugnion (1873) assumed to be developing neuromasts, have been analyzed by light- and electron-microscopy. Their fundamental structure consists of single ampullae with sensory and accessory cells with apical parts that extend into the pit of the ampulla, an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Istenic L,Bulog B

    更新日期:1984-01-01 00:00:00

  • Reduced level of interphotoreceptor retinoid-binding protein (IRBP), a possible cause for retinal degeneration in the Abyssinian cat.

    abstract::Retinae of Abyssinian cats homozygous for a retinal degeneration gene, and normal controls, have been investigated using antibodies directed against opsin, transducin alpha (TD-alpha), S-antigen (48K protein), interphotoreceptor retinoid-binding protein (IRBP), and cone outer segments. IRBP-immunoreactivity (IR) is mu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Narfström K,Nilsson SE,Wiggert B,Lee L,Chader GJ,van Veen T

    更新日期:1989-09-01 00:00:00

  • Quantitative evaluation of myenteric ganglion cells in normal human left colon: implications for histopathological analysis.

    abstract::The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation of myenteric neurons and glial cells in paraffin-embedded colonic ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ippolito C,Segnani C,De Giorgio R,Blandizzi C,Mattii L,Castagna M,Moscato S,Dolfi A,Bernardini N

    更新日期:2009-05-01 00:00:00

  • Visualization of the retino-hypothalamic projection in the rat by cobalt precipitation.

    abstract::The technique of cobalt sulfide precipitation combined with Timm's sulfide-silver method for intensification of heavy metals was used to delineate the retino-hypothalamic projection of the rat. Freshly isolated rat brains were dissected and a solution of cobaltous chloride was applied to one of the cut optic nerves. S...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Mason CA,Lincoln DW

    更新日期:1976-04-28 00:00:00

  • Expression of urocortin and corticotropin-releasing hormone receptors in the horse thyroid gland.

    abstract::Urocortin (UCN) is a 40-amino-acid peptide and a member of the corticotropin-releasing hormone (CRH) family, which includes CRH, urotensin I, sauvagine, UCN2 and UCN3. The biological actions of CRH family peptides are mediated via two types of G-protein-coupled receptors, namely CRH type 1 receptor (CRHR1) and CRH typ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Squillacioti C,De Luca A,Alì S,Paino S,Liguori G,Mirabella N

    更新日期:2012-10-01 00:00:00

  • Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals.

    abstract::The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Friis-Hansen L,Wierup N,Rehfeld JF,Sundler F

    更新日期:2005-07-01 00:00:00

  • An electron microscopic study on stellate cells in the rabbit adenohypophysis under various endocrine conditions.

    abstract::Stellate cells in the rabbit adenohypophysis were observed electron microscopically under normal and experimental conditions such as lactation, thyroidectomy, adrenalectomy, or castration. In control animals stellate cells had a scanty cytoplasm surrounding the nucleus and possessed slender processes extending between...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Shiotani Y

    更新日期:1980-01-01 00:00:00

  • A comparative study on the innervation and the vascularization of the bulbus arteriosus in teleost fish.

    abstract::The structure of the bulbus arteriosus of a wide range of teleost fish is described with particular reference to the vascularization and innervation. The adventitia of the organ consists of blood vessels and large nerve bundles in a collagen matrix. The nerve bundles contain monoamines, and fluorescence studies show s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Watson AD,Cobb JL

    更新日期:1979-02-15 00:00:00

  • Analysis and in situ detection of cholecalcin messenger RNA (9000 Mr CaBP) in the uterus of the pregnant rat.

    abstract::The molecular cloning of a cDNA fragment synthesised from rat duodenal mRNA coding for cholecalcin (calbindin), a 9000 Mr vitamin D-induced calcium-binding protein (CaBP), has been previously described. DNA/RNA hybridisation assays have been used to examine CaBP mRNA production in the uterine horns and duodena of preg...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Warembourg M,Perret C,Thomasset M

    更新日期:1987-01-01 00:00:00

  • VEGF differentially regulates transcription and translation of ZO-1alpha+ and ZO-1alpha- and mediates trans-epithelial resistance in cultured endothelial and epithelial cells.

    abstract::Tight junctions (TJ) between retinal pigmented epithelial (RPE) and retinal endothelial cells maintain the outer and inner blood-retinal barrier, and the breakdown of these barriers is associated with retinal diseases. Vascular endothelial growth factor (VEGF) increases vascular permeability and is thought to be invol...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ghassemifar R,Lai CM,Rakoczy PE

    更新日期:2006-01-01 00:00:00

  • Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta.

    abstract::During pregnancy, the calcium (Ca(2+)) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca(2+) transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca(2+) in placental transport in vivo, we questioned wh...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Belkacemi L,Gariépy G,Mounier C,Simoneau L,Lafond J

    更新日期:2004-01-01 00:00:00

  • Immunocytochemical localization of lipophorins in the flight muscles of the migratory locust (Locusta migratoria) at rest and during flight.

    abstract::Locust lipoproteins (lipophorins) were localized by indirect immunofluorescence- and immunogold labelling in cryosections of dorsolongitudinal flight muscles. Immunolabelling was performed with monoclonal antibodies against apolipoproteins epitopes that are exposed at the surfaces of the lipophorin particles. Both at ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Van Antwerpen R,Linnemans WA,Van der Horst DJ,Beenakkers AM

    更新日期:1988-06-01 00:00:00

  • The fine structure of the ocelli of Schistocerca gregaria. The neural organisation of the synaptic plexus.

    abstract::A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Goodman LJ,Mobbs PG,Kirkham JB

    更新日期:1979-02-28 00:00:00

  • The mature mesonephric nephron of the rabbit embryo. II. TEM-studies.

    abstract::Transmission electron micrographs of the mesonephric nephron in 18 day rabbit embryos reveal major cytological structures reappearing in the nephron of the definitive rabbit kidney. The initial segment of the proximal tubule resembles (despite quite different cell proportions) the cell picture of the metanephric S2-se...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Wettstein R,Tiedemann K

    更新日期:1981-01-01 00:00:00

  • Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events.

    abstract::Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sebrell TA,Sidar B,Bruns R,Wilkinson RA,Wiedenheft B,Taylor PJ,Perrino BA,Samuelson LC,Wilking JN,Bimczok D

    更新日期:2018-02-01 00:00:00

  • Expression and localization of growth hormone and its receptors in the chicken ovary during sexual maturation.

    abstract::Roles of pituitary growth hormone (GH) in female reproduction are well established. Autocrine and/or paracrine actions of GH in the mammalian ovary have additionally been proposed, although whether the ovary is an extra-pituitary site of GH expression in the laying hen is uncertain. This possibility has therefore been...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hrabia A,Paczoska-Eliasiewicz HE,Berghman LR,Harvey S,Rzasa J

    更新日期:2008-05-01 00:00:00

  • Interdigitating cells in the lymphoid tissues of bovine fetuses and calves. An electron-microscopic study.

    abstract::Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bielefeldt Ohmann H,Basse A

    更新日期:1984-01-01 00:00:00

  • Bovine Reissner's fiber (RF) and the central canal of the spinal cord: an immunocytochemical study using a set of monoclonal antibodies against the RF-glycoproteins.

    abstract::The subcommissural organ secretes N-linked complex-type glycoproteins into the cerebrospinal fluid. These glycoproteins condense to form Reissner's fiber (RF), which extends along the fourth ventricle and central canal of the spinal cord. A set of three monoclonal antibodies (Mabs 3E6, 3B1, and 2A5) has been obtained ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pérez J,Garrido O,Cifuentes M,Alonso FJ,Estivill-Torrús G,Eller G,Nualart F,López-Avalos MD,Fernández-Llebrez P,Rodríguez EM

    更新日期:1996-10-01 00:00:00

  • Ultrastructural and cytological changes in the muscle fibers of the pectoralis of the giant Canada goose (Branta canadensis maxima) in disuse atrophy during molt.

    abstract::Adult male Branta canadensis maxima were collected from a nonmigratory feral population during their premolt, molt and postmolt phases. Lean dry weight of the pectoralis muscle decreased significantly (p less than or equal to 0.0001) during molt, as a result of disuse atrophy. Histochemical analysis revealed that the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Rosser BW,George JC

    更新日期:1987-03-01 00:00:00

  • A second cell type in Stannius bodies of two euryhaline telecost species.

    abstract::In the corpuscles of Stannius of sticklebacks and eels two cell types are described of presumably endocrine nature. The predominating type, comparable to the cells observed in other species, responds to variation in calcium content of the medium and possibly produces a hypocalcemic hormone. The second cell type is unr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bonga SE,Greven JA

    更新日期:1975-06-09 00:00:00

  • Three-dimensional architecture of the tubular endocytic apparatus and paramembranous networks of the endoplasmic reticulum in the rat visceral yolk-sac endoderm.

    abstract::The three-dimensional architecture of the tubular endocytic apparatus and the endoplasmic reticulum in the rat yolk-sac endoderm was investigated after loading with horseradish peroxidase-conjugated concanavalin A by intrauterine administration. After 30 min, small vesicles (50-150 nm in diameter), small tubules (80-1...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ichimura T,Hatae T,Sakurai T,Ishida T

    更新日期:1994-11-01 00:00:00

  • Loss of purinergic P2X receptor innervation in human detrusor and subepithelium from adults with sensory urgency.

    abstract::Purinergic P2X receptors associated with the parasympathetic nerves supplying human bladder smooth muscle (detrusor) are implicated in control of detrusor contractility. The relative abundance of all seven subtypes colocalised with synaptic vesicles on parasympathetic nerves was examined in specimens from normal adult...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ray FR,Moore KH,Hansen MA,Barden JA

    更新日期:2003-12-01 00:00:00

  • Localization of hydroxyindole O-methyltransferase-synthesizing cells in bovine epithalamus: immunocytochemistry and in-situ hybridization.

    abstract::Hydroxyindole O-methyltransferase (HIOMT)-immunoreactive cells and melatonin synthesis were demonstrated in bovine epithalamus (including the pineal gland) with monoclonal antibodies and cRNA probes to HIOMT. The HIOMT-immunoreactive product was present in the cytoplasm of pinealocytes. All identifiable pinealocytes w...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sato T,Deguchi T,Ichikawa T,Fujieda H,Wake K

    更新日期:1991-03-01 00:00:00

  • The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa.

    abstract::The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schürmann A,Axer H,Scheepers A,Doege H,Joost HG

    更新日期:2002-02-01 00:00:00

  • Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine.

    abstract::There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fakhry J,Wang J,Martins P,Fothergill LJ,Hunne B,Prieur P,Shulkes A,Rehfeld JF,Callaghan B,Furness JB

    更新日期:2017-08-01 00:00:00

  • Myotendinous junctions of tonic muscle cells: structure and loading.

    abstract::Regions within frog semitendinosus muscle that are rich in tonic muscle cells were identified histochemically by myosin adenosine triphosphatase- and succinic dehydrogenase-staining procedures. Bundles of cells still attached to tendinous insertions were removed from those sites, prepared for electron microscopy and s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Tidball JG,Daniel TL

    更新日期:1986-01-01 00:00:00