Abstract:
:The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized RBL-2H3 cells and 125I goat anti-rabbit IgG. After purification of the serum on a protein G column, RBL-2H3 cells, mouse mast cells, and human basophils showed a doublet at 20 000 kDa. When 125I labeled, saponin-permeabilized cells were immunoprecipitated with the anti-gamma antibody, all 3 subunits of the IgE receptor complex coprecipitated. Furthermore, binding of the antibody to the cell surface did not induce histamine release. By immunofluorescence of fixed cells, the receptor was evenly distributed in the RBL-2H3 cells. Although no capping was observed when the cells were incubated with the antibody prior to fixation, the antibody did stimulate endocytosis when it was bound to the cells at 25 degrees C or at 37 degrees C, but not at 4 degrees C. Binding of the antibody to the RBL-2H3 cells also induced cell spreading and ruffling of the plasma membrane. The results of this study indicate that the gamma subunit of the high affinity IgE receptor may play a role in signal transduction.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Jamur MC,Oliver Cdoi
10.1007/s004410050575subject
Has Abstractpub_date
1996-04-01 00:00:00pages
153-9issue
1eissn
0302-766Xissn
1432-0878journal_volume
284pub_type
杂志文章abstract::NdWFamide is an Aplysia cardioexcitatory tri-peptide containing D-tryptophan. To investigate the roles of this peptide, we examined the immunohistochemical distribution of NdWFamide-positive neurons in Aplysia tissues. All the ganglia of the central nervous system (CNS) contained NdWFamide-positive neurons. In particu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-003-0707-3
更新日期:2003-04-01 00:00:00
abstract::The ultrastructural distribution of fibronectin immunoreactivity was investigated in the chicken embryo during late gastrulation. Sites of binding of anti-fibronectin antibodies were ascribed to the basal lamina and associated structures, and to the cell surface. The fibronectin-rich basal lamina was resolved into a l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217185
更新日期:1985-01-01 00:00:00
abstract::Hypothalamic slices containing the lateral hypothalamic area (LHA) were prepared from 6- to 8-day-old rats and maintained in stationary culture for up to 35 days in order to analyse how well the melanin-concentrating hormone (MCH) neurons survived. As previously reported for other brain areas, this method yielded a lo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051330
更新日期:1999-07-01 00:00:00
abstract::Tactile hairs on the locust thorax can be divided into two classes by their external morphology and their central projection pattern: Short hairs, 10--100 micrometer in length, which are assembled in distinct plates and rows, and long hairs, 100--800 micrometer in length, which are distributed all over the body and ar...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234546
更新日期:1981-01-01 00:00:00
abstract::A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-1036-x
更新日期:2005-09-01 00:00:00
abstract::Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223227
更新日期:1975-01-01 00:00:00
abstract::R*-cells of the digestive gland of Carcinus maenas have been investigated functionally and morphologically. A comparison of the capacity of separated cell suspensions to synthesize glycogen gave support to the hypothesis that R and R* cells belong to the same cell line. The unexpected observation of R* cells in gastri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318696
更新日期:1992-01-01 00:00:00
abstract::Neural lobes of control and "Jimpy" mice were examined electron microscopically. The ultrastructure and incidence of pituicytes was examined following reports of reductions in astrocyte and oligodendrocyte populations in areas of the CNS of Jimpy mice. The failure to demonstrate any modification of structure or number...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220162
更新日期:1976-03-05 00:00:00
abstract::Hydroxyindole O-methyltransferase (HIOMT)-immunoreactive cells and melatonin synthesis were demonstrated in bovine epithalamus (including the pineal gland) with monoclonal antibodies and cRNA probes to HIOMT. The HIOMT-immunoreactive product was present in the cytoplasm of pinealocytes. All identifiable pinealocytes w...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00327275
更新日期:1991-03-01 00:00:00
abstract::During the reproductive period of the male stickleback, structural and functional changes of the kidney take place, both in the glomeruli and in the renal tubule cells. The structural changes in the glomeruli involve almost all glomerular components and point to a reduction of the glomerular filtration rate. The purpo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210146
更新日期:1981-01-01 00:00:00
abstract::Intracellular insulin-binding sites were directly traced in fixed monolayer cultures of a variety of cell types with the use of two fluorescent derivatives of insulin, viz. fluorescein isothiocyanate (FITC)-labelled and tetramethyl rhodamine isothiocyanate (TMRITC)-labelled insulin. Both derivatives retained the prope...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232150
更新日期:1980-01-01 00:00:00
abstract::In plants, intercellular structures named plasmodesmata (PD) form a continuous cytoplasmic network between neighboring cells. PD pores provide channels for intercellular symplasmic (cell-to-cell) transport throughout most tissues of the plant body. Cell-defining proteins, such as transcription factors, and regulatory ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-012-1550-1
更新日期:2013-04-01 00:00:00
abstract::The retina of the distal and proximal lens-bearing complex ocelli are composed of pigmented sensory cells and long pigmented cells. A ciliary sheath from each sensory cell, together with the processes of long pigmented cells, extends through the vitreous layer as far as the capsule that envelops the lens. Each ciliary...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219415
更新日期:1976-07-30 00:00:00
abstract::Doubts exist as to whether afferent nerve fibers exert a neurotrophic effect on the differentiation of sensory cells in the developing vestibular neuroepithelium. To determine whether innervation of hair cells precedes their differentiation, we have used the L1 adhesion molecule as a marker for axons. The detection of...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00229069
更新日期:1989-01-01 00:00:00
abstract::Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual beha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1394-8
更新日期:2012-06-01 00:00:00
abstract::The muscle cells of the ventricle, the branchial heart and the branchial heart appendages of Rossia macrosoma (Delle Chiaje) are studied. The ventricle myocardium has three muscle layers, while the other two organs exhibited a loose arrangement of muscle cells. The muscle cells of the ventricle, the branchial heart an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:
更新日期:1977-12-13 00:00:00
abstract::The luminal surface features and junctional complexes from developing blood vessels in the rat central nervous system have been studied by high-voltage electron microscopy and scanning electron microscopy. Developing blood vessels exhibit three types of luminal projections; marginal folds or ridges at junctional compl...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222417
更新日期:1977-01-04 00:00:00
abstract::Calretinin and calbindin-D28k are two calcium-binding proteins that are present in largely different sets of nerve cells in the central nervous system. Their appearance during development of the chick retina was studied by immunohistochemistry and Western blots. The patterns are mature one day before hatching. Each ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313956
更新日期:1991-05-01 00:00:00
abstract::Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050641
更新日期:1996-08-01 00:00:00
abstract::The genes encoding transmembrane glycoproteins of the cadherin family, i.e., the Ca(2+)-dependent cell-cell adhesion molecules, are typically expressed in cell-type- or cell-lineage-specific patterns. One of them, vascular endothelial (VE)-cadherin, is widely considered to be specific for vascular endothelia in which ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-008-0718-1
更新日期:2009-01-01 00:00:00
abstract::To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233727
更新日期:1980-01-01 00:00:00
abstract::The presence of immunoreactive enkephalin, dynorphin, vasoactive intestinal polypeptide, cholecystokinin, substance P and neuropeptide Y in nerve fibers that project to the guinea-pig inferior mesenteric ganglion was analysed, after different denervation and ligation procedures. A quantitative analysis demonstrates th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219741
更新日期:1988-09-01 00:00:00
abstract::The distribution of FMRFamide-like immunoreactive cell bodies and fibers in the nervous system of the earthworm Lumbricus terrestris has been studied by means of immunocytochemistry. The cerebral ganglion contains 150-170 immunoreactive nerve cells that are organized into six major groups in the rostral and five major...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050843
更新日期:1997-06-01 00:00:00
abstract::Apart from the characteristic and progressive motor- and movement-related problems, Parkinson's disease (PD) patients also suffer from several non-motor symptoms, including gastrointestinal dysfunction. The fact that the enteric nervous system (ENS) controls motility and that one of the typical PD hallmarks, α-synucle...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-018-2856-4
更新日期:2018-07-01 00:00:00
abstract::The circadian timing system is a neural network consisting of the hypothalamic suprachiasmatic nucleus, aided by the retina, other hypothalamic nuclei, autonomic regions of the spinal cord, sympathetic ganglia and the pineal gland. Extensive studies conducted over the last two decades have unravelled the principal ite...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s004410050652
更新日期:1996-09-01 00:00:00
abstract::SCO-spondin is a newly identified protein, strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When secreted into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, it condenses and forms Reissner's fiber. Several conserved domains have previously been charact...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051132
更新日期:1998-09-01 00:00:00
abstract::The cerebral ganglia of the freshwater snail Lymnaea stagnalis contain two clusters of neurosecretory Caudo-Dorsal Cells (CDC). These cells produce a neurohormone which stimulates ovulation. Ganglion transplantation and quantitative electron microscopy show that neuronal isolation of the cerebral ganglia complex (CCC)...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219720
更新日期:1976-04-28 00:00:00
abstract::Hepatic stellate cells become activated and aquire contractility on being cultured. In order to characterize the morphology of contracted and relaxed stellate cells, we performed light- and electron-microscopic analyses of cultured stellate cells on collagen gels. Incubation of stellate cells with medium alone, 10 nM ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050717
更新日期:1996-12-01 00:00:00
abstract::Double-labeling immunofluorescent histochemistry demonstrates that calretinin, a calcium-binding protein, coexists with calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in the fibers innervating the lamina propria of the rat intestinal villi. An acetylcholinesterase histochemical stain r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318366
更新日期:1995-06-01 00:00:00
abstract::The bursa of Fabricius of the chicken is known to be both a primary lymphoid organ and a secondary lymphoid tissue. Bursal follicles are equipped with antigen-trapping follicle-associated epithelium. However, bioactive antigens such as protein and bacteria have not been detected in the bursal parenchyma. By immunopero...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1701-z
更新日期:2013-12-01 00:00:00