Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris.


:A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility, chromatographic elution behaviour, immunological cross-reactivity and identical nucleotide sequence of segments obtained by reverse transcription/polymerase chain reaction. Immunofluorescence studies with smear preparations of developing male germ cells revealed characteristic changes of the local distribution of actin and EWAM-P1 during spermatogenesis. These changes were correlated with the developmental transport processes and structural alterations. F-actin, as revealed by rhodamine-phalloidin staining, formed a toroid-shaped structure in cytoplasmic bridges connecting the germ cells to a central cytophore during the developmental stages. An actin antibody reacting with both G- and F-actin demonstrated that actin was concentrated at the proximal and distal parts of the spermatocytes. EWAM-P1 was also localized in these regions, with intense staining in the distal part of spermatocytes and young spermatids in which the Golgi complex and proacrosome resided. The anti-actin antibody further stained the periphery of the nucleus. This staining gradually reduced during sperm maturation and covered about half of the length of the nucleus in elongated spermatids. Co-localization of EWAM with actin implied a functional significance of this gelsolin-related protein for the rearrangement of the actin cytoskeleton during earthworm spermiogenesis.


Cell Tissue Res


Cell and tissue research


Krüger E,Hinssen H,D'Haese J




Has Abstract


2008-04-01 00:00:00












  • Analysis and in situ detection of cholecalcin messenger RNA (9000 Mr CaBP) in the uterus of the pregnant rat.

    abstract::The molecular cloning of a cDNA fragment synthesised from rat duodenal mRNA coding for cholecalcin (calbindin), a 9000 Mr vitamin D-induced calcium-binding protein (CaBP), has been previously described. DNA/RNA hybridisation assays have been used to examine CaBP mRNA production in the uterine horns and duodena of preg...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Warembourg M,Perret C,Thomasset M

    更新日期:1987-01-01 00:00:00

  • Age-related decline of lymphoid-tissue components in the oral mucosa of the lip, cheek and soft palate of Macaca fascicularis.

    abstract::In three mature monkeys (Macaca fascicularis; 3.5 +/- 0.3 kg in weight), the labial, buccal and soft-palate mucosae were examined morphologically and stereologically. Using fixation by perfusion, standardized methods of tissue preparation and morphometric analysis at the light-microscopic level, the gross dimensions (...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schroeder HE,Dörig-Schwarzenbach A

    更新日期:1984-01-01 00:00:00

  • Ultrastructure of the nongranulated cells and morphology of the extracellular spaces in the pars distalis of adult and pouch-young tammar wallabies (Macropus eugenii).

    abstract::Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Leatherland JF,Renfree MB

    更新日期:1982-01-01 00:00:00

  • Structure of the auditory system of the weta Hemideina crassidens (blanchard, 1851) (Orthoptera, Ensifera, Gryllacridoidea, Stenopelmatidae). 1. Morphology and histology.

    abstract::The morphology and histology of the tibial auditory system of the New Zealand weta, Hemideina crassidens, are described. The groups of acoustic sensilla conform closely to the subgenual organ, intermediate organ and crista acoustica of the Tettigoniidae. Each prothoracic tibia bears two thick (40-100 micrometers) tymp...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ball EE,Field LH

    更新日期:1981-01-01 00:00:00

  • Immunologic localization of alpha- and beta-endorphins and beta-lipotropin in corticotropic cells of the normal and anencephalic fetal pituitaries.

    abstract::The use of antibodies to alpha-(17--39) ACTH, beta-LPH, and alpha- and beta-endorphins has enabled the detection of immunoreactive cells as early as the eight week of fetal life. Immunoreactive cells are located in the anterior and intermediate lobes of the pituitary gland in normal human fetuses. The same cells react...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bégeot M,Dubois MP,Dubois PM

    更新日期:1978-10-30 00:00:00

  • Structure and possible functions of the calcospherite-rich cells (R* cells) in the digestive gland of the shore crab Carcinus maenas.

    abstract::R*-cells of the digestive gland of Carcinus maenas have been investigated functionally and morphologically. A comparison of the capacity of separated cell suspensions to synthesize glycogen gave support to the hypothesis that R and R* cells belong to the same cell line. The unexpected observation of R* cells in gastri...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Loret SM,Devos PE

    更新日期:1992-01-01 00:00:00

  • Neuronal and nonneuronal taurine-like immunoreactivity in the sea pansy, Renilla koellikeri (Cnidaria, Anthozoa).

    abstract::A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Anctil M,Minh CN

    更新日期:1997-04-01 00:00:00

  • A steroid hormone affects sodium channel expression in Manduca central neurons.

    abstract::Neuronal differentiation is characterized by stereotypical sequences of membrane channel and receptor acquisition. This is regulated by the coordinated interactions of a variety of developmental mechanisms, one of which is the control by steroid hormones. We have used the metamorphosis of the holometabolous insect, Ma...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Börner J,Puschmann T,Duch C

    更新日期:2006-07-01 00:00:00

  • Immunolocalization of inhibin alpha-subunit in the human testis. A light- and electron-microscopy study.

    abstract::The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Vannelli GB,Barni T,Forti G,Negro-Vilar A,Vale W,Serio M,Balboni GC

    更新日期:1992-08-01 00:00:00

  • Evidence for protein absorption from the lumen of the seminiferous tubule and rete of the rat testis.

    abstract::As luminal fluid moves from the seminiferous tubule and enters the rete testis, its protein concentration declines from approximately 6 mg/ml to 1 mg/ml. It was therefore suggested that protein is either 1) utilized by the spermatozoa, 2) transported across the epithelium of the terminal segment of the seminiferous tu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hinton BT,Keefer DA

    更新日期:1983-01-01 00:00:00

  • Morphology of tight junctions in the ciliary epithelium of rabbits during arachidonic acid-induced breakdown of the blood-aqueous barrier.

    abstract::A reversible breakdown of the blood-aqueous barrier in the iridial processes of rabbits has been induced by arachidonic acid as demonstrated by the passage of horseradish peroxidase at places through the tight junctions. Freeze-fracture images reveal very discontinuous P-face ridges. However, the analysis of complemen...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Noske W,Hirsch M

    更新日期:1986-01-01 00:00:00

  • Analysis of the expression of p53 during the morphogenesis of the gastroesophageal mucosa of Gallus gallus domesticus (Linnaeus, 1758).

    abstract::Ontogenesis comprises a series of events including cell proliferation and apoptosis and resulting in the normal development of the embryo. Protein p53 has been described as being involved in the development of several animal species. The aim of this study was to analyze the expression of protein p53 during the morphog...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ventura A,do Nascimento AA,dos Santos MA,Vieira-Lopes DA,Sales A,Pinheiro NL

    更新日期:2014-01-01 00:00:00

  • Changes in surviving nerve fibers associated with submucosal arteries following extrinsic denervation of the small intestine.

    abstract::The neuropeptide content of nerve fibers associated with submucosal arteries in the small intestine of guinea pigs was studied in whole-mount preparations using immunohistochemical methods. Tissues were obtained from normal animals or animals in which the small intestine had been extrinsically denervated. In normal an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Galligan JJ,Costa M,Furness JB

    更新日期:1988-09-01 00:00:00

  • Apoptotic and antiapoptotic mechanisms in stroke.

    abstract::Apoptosis is a form of programmed cell death that occurs in neurons during development of the nervous system and may also be a prominent form of neuronal death in chronic neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Recent findings also implicate apoptosis in neuronal degeneration after is...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Mattson MP,Culmsee C,Yu ZF

    更新日期:2000-07-01 00:00:00

  • The zinc iodide-osmium tetroxide (ZIO) reaction on nerve endings in the median eminence of the rat under normal and experimental conditions.

    abstract::The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and rese...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Matsuura T,Ibata Y,Sano Y

    更新日期:1976-10-13 00:00:00

  • Development of striated rootlets during ciliogenesis in the human oviduct epithelium.

    abstract::Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the sam...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hagiwara H,Aoki T,Ohwada N,Fujimoto T

    更新日期:1997-10-01 00:00:00

  • Synaptic input to the axon hillock and initial segment of inhibitory interneurons in the cerebellar cortex of the rat. An electron microscopic study.

    abstract::The axon hillock (AH) and initial segment (IS) of 10 Golgi neurons and 6 basket cells in the cerebellar cortex of the rat were investigated by electron microscopy using serial sections. An average of 10.4 and 11.3 synaptic terminals were observed to establish synaptic contact with the axon hillock region of Golgi and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hámori J

    更新日期:1981-01-01 00:00:00

  • Horseradish peroxidase in axons of the dorsal funiculi of the cat: distribution after an injection of the enzyme into the dorsal column nuclei.

    abstract::Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of R...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Fabricius C,Berthold CH,Gatzinsky KP

    更新日期:1996-01-01 00:00:00

  • The reversible effect of colchicine on the taste bud cells of the central circumvallate papilla in the rat.

    abstract::It is believed that differentiation and maintenance of taste buds in vertebrates is dependent on the trophic function of their sensory nerve supply. In the present work, colchicine was injected into the circumvallate papilla of the rat. This produced a reversible blockade of neuroplasmic transport and disappearance of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Rodrigo Angulo ML,Fernández Sánchez B,Rodríguez-Echandía EL

    更新日期:1978-08-25 00:00:00

  • Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture.

    abstract::In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kikuchi H,Suzuki K,Sakai N,Yamada S

    更新日期:2004-08-01 00:00:00

  • Ontogeny of the GnRH systems in zebrafish brain: in situ hybridization and promoter-reporter expression analyses in intact animals.

    abstract::The ontogeny of two gonadotropin-releasing-hormone (GnRH) systems, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), was investigated in zebrafish (Danio rerio). In situ hybridization (ISH) first detected sGnRH mRNA-expressing cells at 1 day post-fertilization (pf) anterior to the developing olfactory organs. Subseq...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Palevitch O,Kight K,Abraham E,Wray S,Zohar Y,Gothilf Y

    更新日期:2007-02-01 00:00:00

  • Improvement of liver fibrosis by infusion of cultured cells derived from human bone marrow.

    abstract::We develop "autologous bone marrow cell infusion (ABMi) therapy" for the treatment of human decompensated liver cirrhosis and confirm the efficacy and safety of this treatment in multicenter clinical studies. With the goal of further expanding the applications of ABMi, we first cultured human bone marrow cells and the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Tanimoto H,Terai S,Taro T,Murata Y,Fujisawa K,Yamamoto N,Sakaida I

    更新日期:2013-12-01 00:00:00

  • Antennal sensory system of Periplaneta americana L.: distribution and frequency of morphologic types of sensilla and their sex-specific changes during postembryonic development.

    abstract::The morphology of the antennal hair-sensilla of Periplaneta americana, their distribution and frequency on the antennal flagellum have been examined by transmission- and scanning-electron microscopy. The types of sensilla were distinguished with respect to physiologically relevant criteria such as wall structure and n...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schaller D

    更新日期:1978-07-13 00:00:00

  • Cell surface events during resealing visualized by scanning-electron microscopy.

    abstract::The function of exocytosis during plasma membrane resealing might be to facilitate the flow of surface lipid over the disruption site and/or to add defect-spanning "patches" of internal membrane across it. Scanning-electron-microscopic visualization of large plasma membrane disruptions in sea urchin eggs is here used ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: McNeil PL,Baker MM

    更新日期:2001-04-01 00:00:00

  • Distribution of laminin, type IV collagen, and fibronectin in the cell columns and trophoblastic shell of early macaque placentas.

    abstract::The cytotrophoblastic cell columns and trophoblastic shell of macaque placentas accumulate progressively greater amounts of intercellular material during early gestation. We studied the composition of this material in placentas collected from 22-34 days of gestation by using immunoperoxidase techniques directed to the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blankenship TN,Enders AC,King BF

    更新日期:1992-11-01 00:00:00

  • Presence and localization of antithrombin and its regulation after acute lipopolysaccharide exposure in amphioxus, with implications for the origin of vertebrate liver.

    abstract::Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Liang Y,Zhang S,Lun L,Han L

    更新日期:2006-03-01 00:00:00

  • A cytochemical and immunocytochemical study of DNA distribution in spermatid nuclei of mouse, rabbit, and bull.

    abstract::DNA distribution in mouse, rabbit and bull spermatids was analyzed by electron microscopy, after using a Feulgen-like HCl-osmium ammine procedure, and after immunocytochemistry with anti-DNA antibodies. In addition, nucleic acids were visualized with the intercalating dye ethidium bromide and phosphotungstic acid. The...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Courtens JL,Biggiogera M,Fakan S

    更新日期:1991-09-01 00:00:00

  • Quantitation of pulmonary neuroepithelial bodies in pre- and postnatal rabbits.

    abstract::The size, density and total number of neuroepithelial bodies (NEB) in the lungs of late fetal, neonatal, and mature rabbits were determined using fluorescence microscopy. In this study lungs from 27-, 29-, 30-, and 31-day fetuses; neonates of ages 2, 7, and 30 days; and 4- and 7(+)-month-old rabbits, were used. The to...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Redick ML,Hung KS

    更新日期:1984-01-01 00:00:00

  • Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta.

    abstract::During pregnancy, the calcium (Ca(2+)) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca(2+) transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca(2+) in placental transport in vivo, we questioned wh...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Belkacemi L,Gariépy G,Mounier C,Simoneau L,Lafond J

    更新日期:2004-01-01 00:00:00

  • Reduced level of interphotoreceptor retinoid-binding protein (IRBP), a possible cause for retinal degeneration in the Abyssinian cat.

    abstract::Retinae of Abyssinian cats homozygous for a retinal degeneration gene, and normal controls, have been investigated using antibodies directed against opsin, transducin alpha (TD-alpha), S-antigen (48K protein), interphotoreceptor retinoid-binding protein (IRBP), and cone outer segments. IRBP-immunoreactivity (IR) is mu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Narfström K,Nilsson SE,Wiggert B,Lee L,Chader GJ,van Veen T

    更新日期:1989-09-01 00:00:00