Abstract:
:In the present study, we have analyzed the direct effects of cytokines, which mediate the acute-phase response in liver, on connexin expression and gap-junctional intercellular communication in immortalized MHSV12 mouse hepatocytes. When these cells were stimulated for 24 h with interleukin 1 and interleukin 6, the amount of connexin26 (Cx26) mRNA increased together with beta-fibrinogen mRNA, as expected for this positive acute-phase gene. In contrast, connexin32 (Cx32) mRNA expression was not affected under these conditions. Indirect immunfluorescence revealed a drastic decrease in Cx32 signals, whereas slightly more Cx26 signals were found. Stronger stimulation with interleukin 1 and tumor necrosis factor alpha gave a dose-dependent increase in steady state levels of Cx26 and beta-fibrinogen mRNA, but no further change in Cx32 mRNA level was seen. However, when Cx32 protein was analyzed on immunoblots, we found a 5-fold decrease in expression even at low cytokine doses that did not affect Cx32 mRNA expression. Under these conditions, cell to cell transfer of Lucifer yellow, microinjected into immortalized hepatocytes, was decreased by 70%, suggesting that intercellular communication through Cx32 channels was partially inhibited earlier than other genetic alterations characteristic of the acute-phase response. Thus, the major hepatic gap junction protein was largely downregulated at the beginning of the experimental inflammatory reaction, but about 30% of gap-junctional intercellular communication was maintained. This suggests that, during the acute-phase response, the second hepatic Cx26 protein may compensate in part for the downregulation of the Cx32 protein.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Temme A,Traub O,Willecke Kdoi
10.1007/s004410051184subject
Has Abstractpub_date
1998-11-01 00:00:00pages
345-50issue
2eissn
0302-766Xissn
1432-0878journal_volume
294pub_type
杂志文章abstract::Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219221
更新日期:1986-01-01 00:00:00
abstract::In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell-substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp...
journal_title:Cell and tissue research
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doi:10.1007/s00441-015-2122-y
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abstract::Colitis is associated with alterations in electrolyte and water transport. These changes give rise to some of the symptoms experienced by patients with colitis. Alterations in fluid flux may also contribute to increased susceptibility to mucosal injury. Recently, endogenous water channel proteins (aquaporins; AQPs), h...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221868
更新日期:1986-01-01 00:00:00
abstract::This study explores the molecular composition of the tight junction (TJ) in human term placenta from normal women and from patients with preeclampsia, a hypertensive disorder of pregnancy. Maternal endothelial dysfunction is a critical characteristic of preeclampsia; hence, we have analyzed its impact on placental ves...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0135-7
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1788-2
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050622
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journal_title:Cell and tissue research
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217869
更新日期:1984-01-01 00:00:00
abstract::The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218204
更新日期:1987-05-01 00:00:00
abstract::The three-dimensional ultrastructure of the pineal body of the rat is described on the basis of freeze-fractured preparations. The pineal capsule consists of irregular cells with very flat and perforated processes. Through these openings, extremely branched canaliculi, extending to almost every pineal cell, communicat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238052
更新日期:1979-09-02 00:00:00
abstract::During pregnancy, the calcium (Ca(2+)) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca(2+) transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca(2+) in placental transport in vivo, we questioned wh...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-003-0811-4
更新日期:2004-01-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221103
更新日期:1977-04-20 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051230
更新日期:1999-02-01 00:00:00
abstract::The size of various tubes within tubular organs such as the lung, vascular system and kidney must be finely tuned for the optimal delivery of gases, nutrients, waste and cells within the entire organism. Aberrant tube sizes lead to devastating human illnesses, such as polycystic kidney disease, fibrocystic breast dise...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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更新日期:2013-11-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220136
更新日期:1976-02-25 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1144-2
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abstract::In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0543-y
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223265
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abstract::Satellite cells can be isolated from skeletal muscle biopsies, activated to proliferating myoblasts and differentiated into multinuclear myotubes in culture. These cell cultures represent a model system for intact human skeletal muscle and can be modulated ex vivo. The advantages of this system are that the most relev...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1655-1
更新日期:2013-12-01 00:00:00
abstract::It is a well-established fact that the sympathetic, parasympathetic and enteric nervous systems are affected at early stages in Parkinson's disease (PD). However, it is not yet clarified whether the earliest pathological events preferentially occur in any of these three divisions of the autonomic nervous system (ANS)....
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
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更新日期:2018-07-01 00:00:00
abstract::The hormone-induced pigment dispersion in primary cultures of xanthophores of goldfish (Carassius auratus L.) has been shown to involve the dispersion of not only carotenoid droplets but also of smooth endoplasmic reticulum. The dispersion of these organelles is inhibited by cytochalasin B and is accompanied by thinni...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219555
更新日期:1978-07-10 00:00:00
abstract::Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234830
更新日期:1979-04-30 00:00:00
abstract::The distribution of Mg++-ATPase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline pH cytochemical methods at the electron-microscopic level. Reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. Levamisole, ouabain, and va...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218550
更新日期:1986-01-01 00:00:00
abstract::Cobaltous-lysine is transported anterogradely from the optic nerve of the teleost, Lethrinus chrysostomus (Lethrinidae, Perciformes). The marginal optic tract is labelled in longitudinal bands of light and dark staining fibres which persists caudally within the ventral division but not in the dorsal division. This spe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218889
更新日期:1989-01-01 00:00:00
abstract::The attachment of the body of the snail Lymnaea stagnalis to the shell was studied by histochemistry and light and electron microscopy. Muscles of the body wall insert into the connective tissue by way of long thin projections of sarcolemma. The muscle cells end under the basement membrane of a specialised area of the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224663
更新日期:1976-08-26 00:00:00