Immuno-electron-microscopic analysis of the basal route of secretion in the subcommissural organ of the rabbit.

Abstract:

:Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et al. 1981) and, thereafter, (2) particulate gold-marker solution, immunoreactive sites could be clearly visualized within the extracellular matrix of both (a) the basal part of the ependymal cell layer, and (b) the hypendyma proper. Abundant secretory material was identified within (i) dilated intercellular spaces (a + b) as well as (ii) branching basal lamina labyrinths and distinct perivascular spaces (b). All these compartments are thought to belong to a system of extracellular channels, which may function in secretion directed toward hypendymal blood vessels.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Lösecke W,Naumann W,Sterba G

doi

10.1007/BF00219221

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

449-56

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

244

pub_type

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