Abstract：
:Chondrocytes were isolated enzymatically from rabbit ear cartilage, grown in vitro or as autotransplants for 1, 2 or 5 weeks and then examined by transmission electron microscopy. A confluent monolayer formed rapidly in vitro and the cells later grew in multiple overlapping layers, producing thick sheets of cartilaginous tissue. The cells retained a normal structure throughout the period of observation and, like the chondrocytes in intact cartilage, showed numerous microfilaments, and extensive granular endoplasmic reticulum and a prominent Golgi complex. Large amounts of intercellular matrix were laid down in vitro consisting of thin collagen fibrils, small rounded or polygonal granules believed to represent proteoglycans and patches and fibres of elastin. Chondrocytes in intramuscular autotransplants reconstituted an elastic cartilage. The exogenous origin of the cells in the transplants was verified by labeling of the lysosomes by exposure of the cells to colloidal thorium dioxide particles prior to injection. Structurally, the cells and the matrix of the transplants conformed to the above description. Accumulations of elastin-like material were sometimes observed in the Golgi vacuoles of the cells. Extracellularly, such conglomerates aggregated in connection with bundles of microfibrils, building up mature elastic fibres with a dense amorphous structure. The culture and transplant systems characterized here provide suitable experimental models for studies on development, growth and aging of elastic cartilage, including various aspects of the formation and turnover of elastic fibres and other macromolecular matrix components.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Thyberg J,Hinek Adoi
10.1007/BF00227600subject
Has Abstractpub_date
1977-05-31 00:00:00pages
341-56issue
3eissn
0302-766Xissn
1432-0878journal_volume
180pub_type
杂志文章abstract：:Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other form...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01258502
更新日期：1982-01-01 00:00:00
abstract：:Pimozide, a specific blocker of dopaminergic receptors, was injected for 4 or 9 days in freshwater (FW) eels or eels acclimated to sea water (SW) for 10 or 30 days. The daily dose was 100 or 200 microgram/100 g. Melanophore index values increase in FW and in 1 month-SW injected eels. All the treated fish react by a to...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209272
更新日期：1978-05-29 00:00:00
abstract：:Cells binding anti-bovine TASH beta serum were found exclusively in the rostral lobe of the adenohypophysis of the drake using the peroxidase-antiperoxidase complex unlabelled antibody method. The specificity of the binding of the anti-serum to TSH cells was established by relating the morphology and relative abundanc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234834
更新日期：1979-04-30 00:00:00
abstract：:The cellular response of non-neuronal elements of the pyramidal cell layer of the rat hippocampus, especially the area CA3, was observed electron microscopically following destruction of this formation by means of intraventricular administration of kainic acid (KA). The neuroglial cell types responding to the KA-induc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238652
更新日期：1981-01-01 00:00:00
abstract：:Rainbow trout (Oncorhynchus mykiss) were exposed to ion-poor (soft) water to test the hypothesis that naturally induced proliferation of branchial chloride cells causes a thickening of the blood-to-water diffusion barrier. This was achieved by using a combination of scanning and transmission electron-microscopic techn...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050622
更新日期：1996-07-01 00:00:00
abstract：:Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00306123
更新日期：1994-05-01 00:00:00
abstract：:For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a di...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219304
更新日期：1976-06-11 00:00:00
abstract：:Putative neural stem cells have been identified within the enteric nervous system (ENS) of adult rodents and cultured from human myenteric plexus. We conducted studies to identify neural stem cells or progenitor cells within the submucosa of adult human ENS. Jejunum tissue was removed from adult human subjects undergo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1130-9
更新日期：2011-05-01 00:00:00
abstract：:Notch signaling is evolutionarily conserved from Drosophila to human. It plays critical roles in neural stem cell maintenance and neurogenesis in the embryonic brain as well as in the adult brain. Notch functions greatly depend on careful regulation and cross-talk with other regulatory mechanisms. Deregulation of Notc...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-017-2641-9
更新日期：2018-01-01 00:00:00
abstract：:Clusterin (CLU) is an extracellular chaperone protein that is implicated in diverse physiological and pathophysiological cellular processes. CLU expression is upregulated in response to cellular stress and under certain conditions, such as neurodegenerative disease and cancer. CLU primarily functions as a chaperone th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2650-8
更新日期：2017-10-01 00:00:00
abstract：:Despite the common structure of vertebrates, the development of the vertebral column differs widely between teleosts and tetrapods in several respects, including the ossification of the centrum and the function of the notochord. In contrast to tetrapods, vertebral development in teleosts is not fully understood, parti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2404-z
更新日期：2016-10-01 00:00:00
abstract：:The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213841
更新日期：1988-04-01 00:00:00
abstract：:The immunohistochemical distribution of the basement membrane (BM) proteins, laminin and type IV collagen, and interstitial type III collagen was investigated in 12 fetal spleens at the 15th-38th gestational weeks (g.w.) and in spleens of 8 infants from term to 4 years. The results were compared with the distribution ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318766
更新日期：1991-02-01 00:00:00
abstract：:Experimental autoimmune orchitis (EAO) in the rat is the primary chronic animal model for the investigation of one of the main causes of male infertility, viz., testicular inflammation. Dendritic cells (DC) are potent antigen-presenting cells that play a fundamental role in autoimmune disease. We investigated the numb...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0129-5
更新日期：2006-05-01 00:00:00
abstract：:The luminal surface features and junctional complexes from developing blood vessels in the rat central nervous system have been studied by high-voltage electron microscopy and scanning electron microscopy. Developing blood vessels exhibit three types of luminal projections; marginal folds or ridges at junctional compl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222417
更新日期：1977-01-04 00:00:00
abstract：:Changes in the frequency of endocrine cells are evidence of intestinal adaptation to germ-free (GF) status. Not only the distribution of these cells along the intestine, but also the differences in intracellular content of these regulatory peptides may be explored to explain functional and structural aspects of GF int...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000323
更新日期：2001-03-01 00:00:00
abstract：:Chorionic binucleate cells (BNC) occur in several ruminants including cow, deer, goat and sheep. They migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. We have compared the BNC of near-term, resin-embedded, ovine and bovine ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00331380
更新日期：1994-12-01 00:00:00
abstract：:The submandibular gland (SMG) of newborn mice has no mature acini but has the rudiments of acini called terminal tubules (TT). The TT are composed of TT cells with dark secretory granules and proacinar cells with lighter secretory granules, the latter being considered the immediate precursor of mature acinar cells. TT...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03205-w
更新日期：2020-08-01 00:00:00
abstract：:Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0430-6
更新日期：2007-09-01 00:00:00
abstract：:Estradiol injections increase serum level of calcium, amino acid, glucose, protein, ammonia and creatinine in immature Epinephelus akaara, and also increase levels of total lipid, cholesterol, phospholipid and esterified fatty acids. Hepatic protein, glycogen and lipid concentrations also rise after estradiol treatmen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217235
更新日期：1984-01-01 00:00:00
abstract：:Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0705-6
更新日期：2008-12-01 00:00:00
abstract：:Dietary restriction (DR) retards physical growth, resulting in small body size, reduced liver weight and reduced number of hepatocytes in rats. We examined the effects of DR on proliferation and apoptosis of hepatocytes during development and explained these changes subcellularly using immunohistochemistry for bromode...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900126
更新日期：2000-03-01 00:00:00
abstract：:Uptake of uteroglobin (UGL) by day-6 rabbit blastocysts and the intracellular fate of this protein were studied by light- and electron-microscopic autoradiography, immunocytochemistry and acid-phosphatase cytochemistry. UGL, labelled with N-succinimidyl-(2-3-3H)-propionate, was administered to embryos in vitro for 25 ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305254
更新日期：1990-12-01 00:00:00
abstract：:The chemical composition of intermediate filaments (IF's) in the ependyma of the subcommissural organ (SCO) of the Mongolian gerbil (Meriones unguiculatus) was investigated immunohistochemically in paraffin-embedded tissue. Antibodies against glial fibrillary acidic protein (GFAP), vimentin, neurofilament proteins and...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218796
更新日期：1989-03-01 00:00:00
abstract：:The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated fr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051160
更新日期：1998-10-01 00:00:00
abstract：:The hormone-induced pigment dispersion in primary cultures of xanthophores of goldfish (Carassius auratus L.) has been shown to involve the dispersion of not only carotenoid droplets but also of smooth endoplasmic reticulum. The dispersion of these organelles is inhibited by cytochalasin B and is accompanied by thinni...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219555
更新日期：1978-07-10 00:00:00
abstract：:In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1777-5
更新日期：2014-04-01 00:00:00
abstract：:The periodontal ligament (PDL) is a highly specialized tissue connecting the cementum with the tooth socket bone and affects the life span of the tooth. However, little is known about the precise characteristics and regenerative mechanism of PDL cells because of the absence of specific markers and cell lines. Therefor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0101-4
更新日期：2006-04-01 00:00:00
abstract：:Electronmicroscopic and cytochemical studies were performed to localize interdigitating reticulum cells (IDC) in the popliteal lymph node of the rat. The morphological features of the IDC of the rat correspond to those described for other species, but also show similarities to normal macrophages in the rat. This is co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220109
更新日期：1976-07-20 00:00:00
abstract：:Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219019
更新日期：1986-01-01 00:00:00
