Ultrastructural immunocytochemical localization of L-glutamate decarboxylase and GABA in rat pancreatic zymogen granules.

Abstract:

:The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar cell, GABA and its biosynthetic enzyme, GAD, were localized in zymogen granules. Quantitative analysis of the GABA immunoreactivity in the acinar cell revealed 1.7 +/- 0.5 gold particles/micron2 over the cytoplasm, 36.6 +/- 14.1 gold particles/micron2 over the zymogen granules, and 2.9 +/- 2.1 gold particles/micron2 over the mitochondria. Quantitative analysis of the distribution of colloidal gold particles, representing glutamate decarboxylase immunoreactivity in the acinar cells, revealed 38.4 +/- 2.5 gold particles/micron2 over the zymogen granules, 4.7 +/- 1.1 gold particles/micron2 over the mitochondria and 6.3 +/- 0.5 gold particles/micron2 over the remainder of the cytoplasm. Substitution of normal sheep serum for the sheep anti-glutamate decarboxylase serum revealed a significant (p less than 0.001) decrease of the colloidal gold particle distribution over the zymogen granules and cytoplasmic compartments of the acini. Gamma aminobutyrate-alpha-ketoglutarate transaminase, the catabolic enzyme for GABA, was not detected in the mitochondria, zymogen granules, and cytoplasm of the acinar cell, suggesting that GABA is not catabolized within the acinar cell. Preabsorption and substitution controls resulted in an absence of labeling. These results suggest that GABA may act extracellularly and/or have a role within the zymogen granule in the exocrine pancreas.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Garry DJ,Garry MG,Sorenson RL

doi

10.1007/BF00213841

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

191-7

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

252

pub_type

杂志文章
  • Midbrain-derived neural stem cells: from basic science to therapeutic approaches.

    abstract::Neural stem cells (NSCs) are a subtype of tissue-specific progenitor cells capable of extended self-renewal and the ability to generate all major cell types of nervous tissue, such as neurons, astroglia and oligodendroglial cells. Recent studies suggest that salient patterning in anterior-posterior and dorsal-ventral ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-004-0923-5

    authors: Storch A,Sabolek M,Milosevic J,Schwarz SC,Schwarz J

    更新日期:2004-10-01 00:00:00

  • Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein.

    abstract::Prolonged secretory stimulation of the exocrine pancreas in the rat by in vivo infusion of caerulein leads to a rapid degranulation of the organ associated with a progressive reduction in the size of the zymogen granules. During the first six to twelve hours of stimulation Golgi complexes are enlarged and several stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00224474

    authors: Bieger W,Martin-Achard A,Bassler M,Kern HF

    更新日期:1976-01-28 00:00:00

  • Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats.

    abstract::PU.1, which is a transcription factor, promotes the terminal differentiation of alveolar macrophages (AMs). Its expression is regulated by granulocyte/macrophage colony-stimulating factor (GM-CSF). In this study of AMs in newborn rats, we performed immunohistochemical staining, acid phosphatase staining, reverse trans...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-007-0405-7

    authors: Iwabuchi H,Kawasaki T,Yamamoto T,Uchiyama M,Nakata K,Naito M

    更新日期:2007-07-01 00:00:00

  • High resolution radioautographic study of newly formed protein in striated muscle with emphasis on red and white fibres.

    abstract::The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were compa...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00209040

    authors: Dadoune JP,Terquem A,Alfonsi MF

    更新日期:1978-10-17 00:00:00

  • Visualization of the retino-hypothalamic projection in the rat by cobalt precipitation.

    abstract::The technique of cobalt sulfide precipitation combined with Timm's sulfide-silver method for intensification of heavy metals was used to delineate the retino-hypothalamic projection of the rat. Freshly isolated rat brains were dissected and a solution of cobaltous chloride was applied to one of the cut optic nerves. S...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219728

    authors: Mason CA,Lincoln DW

    更新日期:1976-04-28 00:00:00

  • Proteases and the gut barrier.

    abstract::Serine proteases, cysteine proteases, aspartic proteases and matrix metalloproteinases play an essential role in extracellular matrix remodeling and turnover through their proteolytic action on collagens, proteoglycans, fibronectin, elastin and laminin. Proteases can also act on chemokines, receptors and anti-microbia...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-012-1390-z

    authors: Biancheri P,Di Sabatino A,Corazza GR,MacDonald TT

    更新日期:2013-02-01 00:00:00

  • Ontogeny of endocrine cells in the respiratory system of Syrian golden hamsters. I. Larynx and trachea.

    abstract::The ontogeny of protein gene product 9.5 (PGP 9.5), serotonin (5-HT), calcitonin gene-related peptide (CGRP), and calcitonin (CT) immunoreactivity was evaluated in small-granule endocrine cells of hamster laryngotracheal epithelium from fetal day 11 to adulthood. Two centrifugal (proximal-to-distal) patterns of differ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00305382

    authors: McDowell EM,Sorokin SP,Hoyt RF Jr

    更新日期:1994-01-01 00:00:00

  • Plasma membrane localization of the GFL receptor components: a nexus for receptor crosstalk.

    abstract::The glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) comprise a group of four homologous and potent growth factors that includes GDNF, neurturin (NRTN), artemin (ARTN), and persephin (PSPN). The survival, growth, and mitotic activities of the GFLs are conveyed by a single receptor tyrosine kina...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03235-4

    authors: Donnelly CR,Pierchala BA

    更新日期:2020-10-01 00:00:00

  • Cellular and subcellular distribution of the calcium-binding protein NCS-1 in the central nervous system of the rat.

    abstract::NCS-1 (neuronal calcium sensor) is a recently characterized member of a highly conserved neuron-specific family of calcium-binding proteins, which also includes frequenin and recoverin. The cellular and subcellular distributions of NCS-1 in the rat nervous system were investigated using light- and electron-microscopic...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051246

    authors: Martone ME,Edelmann VM,Ellisman MH,Nef P

    更新日期:1999-03-01 00:00:00

  • The Canoe protein is necessary in adherens junctions for development of ommatidial architecture in the Drosophila compound eye.

    abstract::Rhabdomeres of the Drosophila melanogaster canoemisl mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the su...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900107

    authors: Matsuo T,Takahashi K,Suzuki E,Yamamoto D

    更新日期:1999-12-01 00:00:00

  • Ecto-ATPase activity in the rat cardiac muscle: biochemical characteristics and histocytochemical localization.

    abstract::We used a combined biochemical and histocytochemical approach to study ecto-ATPase in the rat cardiac muscle. The reaction medium employed for histocytochemical detection was optimized in biochemical assays to achieve the highest enzyme activity and lowest inhibition by the capture agent used for visualization of the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900105

    authors: Zinchuk VS,Okada T,Kobayashi T

    更新日期:1999-12-01 00:00:00

  • Homing of germinal-center cells into germinal centers of lymph node via afferent lymphatics. An autoradiographic study in rabbits.

    abstract::Affinity of lymphoid cells for the microenvironment of germinal centers (GC), as detectable in transfer experiments by rapid homing in spleen GC from the blood, is a capacity expressed by only a subset of lymphoid cells, in particular by those constituting a GC. However, when introduced into the blood stream, these ce...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00215160

    authors: Deenen GJ,Opstelten D,Nieuwenhuis P

    更新日期:1984-01-01 00:00:00

  • Localisation of substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat: a light- and electron-microscopic study.

    abstract::The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunorea...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00354796

    authors: Zhang YL,Tan CK,Wong WC

    更新日期:1994-04-01 00:00:00

  • Light- and electron-microscopic study of the distribution of axons containing substance P and the localization of neurokinin-1 receptor in bone.

    abstract::Substance P (SP) is a neuropeptide that is released from axons of sensory neurons and causes signal transduction through the activation of the neurokinin-1 receptor (NK1-R). The present study demonstrates the distribution of SP-like-immunoreactive (SP-LI) axons and the localization of NK1-Rs in rat bone tissue using t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051100

    authors: Goto T,Yamaza T,Kido MA,Tanaka T

    更新日期:1998-07-01 00:00:00

  • From head to toe: conservation of molecular signals regulating limb and craniofacial morphogenesis.

    abstract::Recent evidence indicates that many molecules involved in generating and patterning the limbs also play a role during craniofacial morphogenesis. On the surface, this is an unexpected finding given that these regions of the body have separate evolutionary origins, are composed of different embryonic tissues, and are q...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410051271

    authors: Schneider RA,Hu D,Helms JA

    更新日期:1999-04-01 00:00:00

  • Deleterious effects of intestinal ischemia/reperfusion injury in the mouse enteric nervous system are associated with protein nitrosylation.

    abstract::Changes in intestinal function, notably impaired transit, following ischemia/reperfusion (I/R) injury are likely to derive, at least in part, from damage to the enteric nervous system. Currently, there is a lack of quantitative data and methods on which to base quantitation of changes that occur in enteric neurons. In...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-1126-x

    authors: Rivera LR,Thacker M,Pontell L,Cho HJ,Furness JB

    更新日期:2011-04-01 00:00:00

  • Tormogen cell and receptor-lymph space in insect olfactory sensilla. Fine structure and histochemical properties in Calliphora.

    abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00209140

    authors: Gnatzy W,Weber KM

    更新日期:1978-06-08 00:00:00

  • Live imaging analysis of human gastric epithelial spheroids reveals spontaneous rupture, rotation and fusion events.

    abstract::Three-dimensional cultures of primary epithelial cells including organoids, enteroids and epithelial spheroids have become increasingly popular for studies of gastrointestinal development, mucosal immunology and epithelial infection. However, little is known about the behavior of these complex cultures in their three-...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-017-2726-5

    authors: Sebrell TA,Sidar B,Bruns R,Wilkinson RA,Wiedenheft B,Taylor PJ,Perrino BA,Samuelson LC,Wilking JN,Bimczok D

    更新日期:2018-02-01 00:00:00

  • Occurrence of neurotrophin receptors and transmitters in the developing Xenopus gut.

    abstract::The ontogeny of gut innervation in the anuran amphibian Xenopus laevis was studied using immunohistochemistry on sections of whole larvae from NF stages 38-52. Immunoreactivity to acetylated tubulin confirmed the presence of nerve fibres as early as stages 38-39. Actin immunoreactivity was found at stage 41, indicatin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410100423

    authors: Holmberg A,Hägg U,Fritsche R,Holmgren S

    更新日期:2001-10-01 00:00:00

  • Immunohistochemical evidence for different pathways immunoreactive to substance P and calcitonin gene-related peptide (CGRP) in the guinea-pig stellate ganglion.

    abstract::The colocalization of immunoreactivities to substance P and calcitonin gene-related peptide (CGRP) in nervous structures and their correlation with other peptidergic structures were studied in the stellate ganglion of the guinea pig by the application of double-labelling immunofluorescence. Three types of fibre were d...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318563

    authors: Heym C,Liu N,Gleich A,Oberst P,Kummer W

    更新日期:1993-06-01 00:00:00

  • Trk neurotrophin receptor-like proteins in the teleost Dicentrarchus labrax.

    abstract::In recent years, data have accumulated suggesting that the role of neurotrophins and Trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. This study was designed to map the expression of immunorea...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000181

    authors: Hannestad J,Marino F,Germanà A,Catania S,Abbate F,Ciriaco E,Vega JA

    更新日期:2000-04-01 00:00:00

  • Pituitary adenylate cyclase-activating polypeptide in intrinsic and extrinsic nerves of the rat pancreas.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) is the latest member of the vasoactive intestinal polypeptide (VIP) family of neuropeptides present in nerve fibres in many peripheral organs. Using double immunohistochemistry, with VIP as a marker for intrinsic innervation and calcitonin-gene related peptide...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004419900124

    authors: Hannibal J,Fahrenkrug J

    更新日期:2000-01-01 00:00:00

  • Lead nitrate and gadolinium chloride administration modify hepatocyte cell surfaces.

    abstract::Modifications of hepatocyte cell surface were determined after single i.v. injection to rats of Pb(NO(3))(2) (known to induce liver hyperplasia followed by apoptosis) or GdCl(3) (known to induce proliferation of parenchymal cells and Kupffer cell depletion) or administration of GdCl(3) 24 h before Pb(NO(3))(2) injecti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-003-0708-2

    authors: Pagliara P,Chionna A,Carlà EC,Caforio S,Dini L

    更新日期:2003-04-01 00:00:00

  • Contrasting effects of ACTH and cyanoketone on delta 5-3 beta-hydroxysteroid dehydrogenase activity in interrenal glands of tadpoles of Rana catesbeiana in vitro.

    abstract::The present communication describes an investigation of stimulation and inhibition of delta 5-3 beta-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of delta 5-3 beta-HSD activity. Co...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00218950

    authors: Yu NW,Ku HH,Chang LT,Hsu CY

    更新日期:1987-12-01 00:00:00

  • Two-color double-labeling in situ hybridization of whole-mount Hydra using RNA probes for five different Hydra neuropeptide preprohormones: evidence for colocalization.

    abstract::The freshwater polyp Hydra magnipapillata has a primitive nervous system that produces at least three distinct classes of neuropeptides: various peptides having the C-terminal sequence Arg-Phe-NH2 (the Hydra-RFamide family), Leu-Trp-NH2 (the Hydra-LWamide family), and a single peptide having the C-terminal sequence Ly...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000240

    authors: Hansen GN,Williamson M,Grimmelikhuijzen CJ

    更新日期:2000-08-01 00:00:00

  • Ecdysteroid binding sites localized by autoradiography in the central nervous system of precommitment fifth-stadium Manduca sexta larvae.

    abstract::Brains and subesophageal ganglia from day 3.5 fifth stadium larvae of Manduca sexta were incubated in vitro with 4 nM tritiated ponasterone A, a 20-hydroxyecdysone analog, to determine whether uptake and specific binding of ecdysteroids occur at a cellular level. These tissues, which were taken just prior to the commi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318414

    authors: Bidmon HJ,Stumpf WE,Granger NA

    更新日期:1991-01-01 00:00:00

  • A scanning electron microscopic study of the rat liver sinusoid: endothelial and Kupffer cells.

    abstract::The surface ultrastructure of Kupffer cells in the rat liver has been studied by scanning electron microscopy (SEM). The results demonstrate that Kupffer cells are both significantly different and clearly distinct from endothelial cells. Kupffer cells have neither pores (and/or "sieve plates") nor fenestrations, all o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00223015

    authors: Motta P

    更新日期:1975-12-10 00:00:00

  • Absolute volume of differentiating cells in the epithelium of the human hard palate.

    abstract::In previous studies the differentiation of the epithelium in the human hard palate has been described stereologically using parameters expressed per unit tissue volume. Since single epithelial cells represent the true biological units of this tissue, it became necessary to estimate the absolute size of such cells in o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216577

    authors: Müller-Glauser W

    更新日期:1981-01-01 00:00:00

  • A study of TSH-synthesis of spontaneous hypertensive rats by electron microscopic morphometry and autoradiography.

    abstract::In order to compare the functional state of the anterior pituitary of spontaneously hypertensive rats (SHR) with that of nonmotensive Wistar Kyoto rats (WKR), the anterior pituitary was examined by morphometry and autoradiography at the level of electron microscopy. The relative number and the relative volume of thyro...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00237005

    authors: Fujiwara T

    更新日期:1979-10-01 00:00:00

  • Effect of hydrocortisone, reserpine, propranolol and phentolamine on in vivo uptake of exogenous amines by adrenal chromaffin cells.

    abstract::An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone ahd no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216742

    authors: Kent C,Monkhouse WS,Coupland RE

    更新日期:1981-01-01 00:00:00