Abstract:
:Uptake of uteroglobin (UGL) by day-6 rabbit blastocysts and the intracellular fate of this protein were studied by light- and electron-microscopic autoradiography, immunocytochemistry and acid-phosphatase cytochemistry. UGL, labelled with N-succinimidyl-(2-3-3H)-propionate, was administered to embryos in vitro for 25 min to 4 h. The kinetics, determined from light-microscopic autoradiographs, showed a continuous uptake of the labeled protein over a 4-h period of incubation. At the ultrastructural level, increasing numbers of silver grains and an intense UGL immunoreaction in protein vacuoles and crystalloid bodies of trophoblast cells indicated that 3H-UGL had accumulated in these organelles. The presence of crystalloid inclusions in protein vacuoles suggests their origin by a condensation of the protein content, including UGL. Lysosomes containing radioactivity were rarely found, suggesting a very low degradation rate of the 3H-UGL. Protein vacuoles and crystalloid bodies exhibited no acid phosphatase reaction. The enzyme was mainly found outside the basal and lateral cell membranes of trophoblast cells, and on the rough endoplasmic reticulum of endoderm cells.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Dannhorn DR,Kirchner Cdoi
10.1007/BF00305254subject
Has Abstractpub_date
1990-12-01 00:00:00pages
569-77issue
3eissn
0302-766Xissn
1432-0878journal_volume
262pub_type
杂志文章abstract::Ontogenetic development of GRF-containing neurons in the rat hypothalamus was studied employing antisera which were generated against hpGRF (1-44)NH2 and rhGRF(1-43)OH: anti-hpGRF-C and -rhGRF sera recognize the species-specific C-terminal portions of the peptides, and anti-hpGRF-MC and -N sera recognize hpGRF(27-44)N...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225416
更新日期:1985-01-01 00:00:00
abstract::Recent studies have accomplished the establishment of a collagenous fiber-fringe matrix upon dental root surfaces in vitro. The present study was undertaken to follow the development of such a matrix in vitro and to test the possible effects of root surface treatments upon this matrix. Periodontal ligament cells, 0.1 ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223152
更新日期:1989-10-01 00:00:00
abstract::Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050641
更新日期:1996-08-01 00:00:00
abstract::The muscle cells of the ventricle, the branchial heart and the branchial heart appendages of Rossia macrosoma (Delle Chiaje) are studied. The ventricle myocardium has three muscle layers, while the other two organs exhibited a loose arrangement of muscle cells. The muscle cells of the ventricle, the branchial heart an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:
更新日期:1977-12-13 00:00:00
abstract::MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00645047
更新日期:1992-12-01 00:00:00
abstract::The development of the heterophil granulocytes in the bone marrow of the guinea pig is described. During the maturation of these cells, three types of granule are formed, not only the azurophil and specific granules already described in other mammals but also a third type of granule referred to here as the nucleated g...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220388
更新日期:1978-11-20 00:00:00
abstract::The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunorea...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00354796
更新日期:1994-04-01 00:00:00
abstract::The elaboration and distribution of newly formed proteins in the striated muscle of 21-day-old mice were investigated by quantitative radioautography at intervals between 2 and 240 min after intravenous injection of tritiated leucine. In radioautographs, the localization and the relative label concentration were compa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209040
更新日期:1978-10-17 00:00:00
abstract::The cytotrophoblastic cell columns and trophoblastic shell of macaque placentas accumulate progressively greater amounts of intercellular material during early gestation. We studied the composition of this material in placentas collected from 22-34 days of gestation by using immunoperoxidase techniques directed to the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00328009
更新日期:1992-11-01 00:00:00
abstract::The vacuoles occurring in rat hepatocytes after intraportal injection of retinol (33 or 67 micrograms) were examined immunohistochemically using respective antibodies against rat albumin, human retinol-binding protein, human ceruloplasmin, human alpha 1-antitrypsin, human transferrin, and human prealbumin as represent...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00261829
更新日期:1989-08-01 00:00:00
abstract::The hormone-induced pigment dispersion in primary cultures of xanthophores of goldfish (Carassius auratus L.) has been shown to involve the dispersion of not only carotenoid droplets but also of smooth endoplasmic reticulum. The dispersion of these organelles is inhibited by cytochalasin B and is accompanied by thinni...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219555
更新日期:1978-07-10 00:00:00
abstract::N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214676
更新日期:1987-10-01 00:00:00
abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238295
更新日期:1983-01-01 00:00:00
abstract::We report here on cholinergic neurons in the rat hippocampal formation that were identified by immunocytochemistry employing a monoclonal antibody against choline acetyltransferase (ChAT), the acetylcholine-synthesizing enzyme. In general, ChAT-immunoreactive cells were rare, but were observed in all layers of the hip...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215891
更新日期:1986-01-01 00:00:00
abstract::A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03241-6
更新日期:2020-11-01 00:00:00
abstract::The distribution pattern of adrenergic fibres innervating the ocular choroid membrane of the chicken was studied by means of fluorescence and electron microscopy. In addition, the origin of these fibres was investigated after superior cervical ganglionectomy. Adrenergic axons reach the choroid, partly forming the peri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213222
更新日期:1982-01-01 00:00:00
abstract::The distribution and abundance of nitric oxide synthase (NOS)-containing neurons and their terminals in the gastrointestinal tract of the guinea-pig were examined in detail using NADPH diaphorase histochemistry and NOS immunohistochemistry. NOS-containing cell bodies were found in the myenteric plexus throughout the g...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00303090
更新日期:1994-07-01 00:00:00
abstract::In the present study, we characterized intra-epithelial leukocytes in the digestive tract of chickens during postnatal development. Their phenotype was characterized by monoclonal antibodies in cryostat sections and the numbers of the different cell-types were counted in the epithelium of the esophagus, proventriculus...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318748
更新日期:1993-11-01 00:00:00
abstract::The genes encoding transmembrane glycoproteins of the cadherin family, i.e., the Ca(2+)-dependent cell-cell adhesion molecules, are typically expressed in cell-type- or cell-lineage-specific patterns. One of them, vascular endothelial (VE)-cadherin, is widely considered to be specific for vascular endothelia in which ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-008-0718-1
更新日期:2009-01-01 00:00:00
abstract::A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234742
更新日期:1979-02-28 00:00:00
abstract::The innervation of the uterus in the ovoviviparous urodele Salamandra salamandra was studied. In whole mount preparations of the thin-walled uterus of pregnant females, a dense adrenergic network was demonstrated using a modified glyoxylic acid fluorescence technique. Based on vesicle type and cytochemical reactivity ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213797
更新日期:1983-01-01 00:00:00
abstract::In cattle, the oviduct plays a major role in the reproductive process; however, molecular control of oviduct receptivity to the embryo is poorly understood. A model for receptivity based on size of the pre-ovulatory follicle (POF) was used to compare oviductal morphology, cellular proliferation, and candidate transcri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-017-2666-0
更新日期:2017-11-01 00:00:00
abstract::The position, structure and function of the valves within the lateral sinus of the medical leech, Hirudo medicinalis, are described on the basis of vital, light- and electron microscopy. In this species the valvular apparatus consists of multiple elongated fir cone-shaped fibrous villi surrounding the orifices of the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00399522
更新日期:1976-09-20 00:00:00
abstract::1,25-Dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 have antagonistic effects on the acellular bone of the tilapia Sarotherodon mossambicus. 1,25-Dihydroxyvitamin D3 inhibits the activity of the lining osteoblasts. Prolonged administration leads to demineralization of the bone matrix. Injection of 24,25-dihydroxyvi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00206270
更新日期:1983-01-01 00:00:00
abstract::Serial sections of human vaginal and keratinized oral-gingival epithelia were investigated for ciliary structures. Most melanocytes of the gingival epithelium lacked cilia, whereas almost all basal keratinocytes of the deeper portion of the epithelial ridges possessed one cilium each. In the suprabasal layers of the r...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215830
更新日期:1988-02-01 00:00:00
abstract::A freeze-etch replica method combined with biochemical analyses was used to investigate the ultrastructural organization of the bovine Descemet's membrane. The freeze-etch replica observations revealed that the intact Descemet's membranes were composed of stacks of two-dimensionally arranged hexagonal lattices, in whi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218356
更新日期:1982-01-01 00:00:00
abstract::Gastrin stimulates gastric acid secretion by acting on the cholecystokinin B/gastrin receptor (CCK-BR). The localization of this receptor at the cellular level showed conflicting results in animal studies and has not been described in man by immunohistochemistry. The aim of the present study is to characterize the pre...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004419900114
更新日期:2000-02-01 00:00:00
abstract::Although the function and effects of many growth factors and extracellular matrix (ECM) molecules have been described for several periodontal tissues in vivo and in vitro, the molecular interactions involved in the communication between cells of the periodontal ligament and the alveolar bone are poorly understood. To ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-1026-z
更新日期:2005-03-01 00:00:00
abstract::Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1839-3
更新日期:2014-07-01 00:00:00
abstract::Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer's patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such prote...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2342-1
更新日期:2016-06-01 00:00:00