Abstract：
:Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage proteins. A range of concentrations (0.1-100 nM) was applied to fibrochondrocytes in monolayer and evaluated after 24 h and after 4 days. High-dose staurosporine treatment (10-100 nM) increased cartilage oligomeric matrix protein 60- to 500-fold and aggrecan gene expression two-fold. This effective range of staurosporine was then applied to scaffoldless tissue-engineered fibrochondrocyte constructs for 4 weeks. Whereas glycosaminoglycan synthesis was not affected, collagen content doubled, from 27.6 +/- 8.8 microg in the untreated constructs to 55.2 +/- 12.2 microg per construct with 100 nM treatment. When analyzed for specific collagens, the 10-nM group showed a significant increase in collagen type I content, whereas collagen type II was unaffected. A concomitant dose-dependent reduction was noted in construct contraction, reflecting the actin-disrupting action of staurosporine. Thus, staurosporine increases the gene expression for important matrix proteins and can be used to enhance matrix production and reduce contraction in tissue-engineered fibrocartilage constructs.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Hoben GM,Athanasiou KAdoi
10.1007/s00441-008-0705-6subject
Has Abstractpub_date
2008-12-01 00:00:00pages
469-76issue
3eissn
0302-766Xissn
1432-0878journal_volume
334pub_type
杂志文章abstract：:The saccular membranes of trout (Oncorhynchus mykiss) and turbot (Scophthalmus maximus) were examined to characterize specialized epithelial cells that might be responsible for ion exchange. The approach for localizing cell types was new for this tissue, as observations were made with a stereomicroscope and a light mi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050851
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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journal_title：Cell and tissue research
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更新日期：2003-08-01 00:00:00
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journal_title：Cell and tissue research
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doi：10.1007/BF00218294
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216519
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218307
更新日期：1987-02-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1994-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236920
更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219403
更新日期：1976-08-20 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00312830
更新日期：1993-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051119
更新日期：1998-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302731
更新日期：1993-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219848
更新日期：1977-04-29 00:00:00
abstract：:In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00238295
更新日期：1983-01-01 00:00:00
abstract：:Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1113-2
更新日期：2011-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051330
更新日期：1999-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226665
更新日期：1977-11-30 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1043-z
更新日期：2011-01-01 00:00:00
