Abstract：
:We have developed a simple and reliable method of preserving antigen immunoreactivity with concomitant excellent retention of the cell ultrastructure. Using this method, we have been able to follow the origin and developmental stages of nuage accumulations within the nurse cell/oocyte syncytium in the ovary of the fruit fly, Drosophila melanogaster, at the ultrastructural level. We have found two morphologically and biochemically distinct forms of nuage material in the nurse cell cytoplasm: translocating accumulations of nuage containing the Vasa protein, termed sponge bodies and stationary polymorphic accumulations of nuage enriched in Argonaute and Survival of motor neuron proteins. Immunogold labeling combined with confocal fluorescent and ultrastructural analyses have revealed that the Vasa-containing nuage accumulations remain closely associated with the cisternae of the endoplasmic reticulum throughout their lifetimes. The migration mechanism of the Vasa-positive nuage appears distinct from the microtubule-dependent translocation of oskar ribonucleoprotein complexes. We postulate that these two distinct nuage translocation pathways converge in the formation of the polar granules within the polar/germ plasm of the oocyte posterior pole. We also provide morphological and immunocytochemical evidence that these polymorphic nuage accumulations correspond to the recently described cytoplasmic domains termed U body-P body complexes.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Jaglarz MK,Kloc M,Jankowska W,Szymanska B,Bilinski SMdoi
10.1007/s00441-011-1145-2subject
Has Abstractpub_date
2011-04-01 00:00:00pages
169-81issue
1eissn
0302-766Xissn
1432-0878journal_volume
344pub_type
杂志文章abstract：:The fate of the major connective tissue polysaccharide hyaluronan, as it appears after release from the matrix, was studied in the Atlantic cod by use of subcutaneous administration of hyaluronan conjugated with fluorescein isothiocyanate (FITC) and labelled with 125I. After administration, the ligand was transported ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050912
更新日期：1997-10-01 00:00:00
abstract：:The livers of Xenopus laevis, grouped by chronological age (0.5, 2 and 3 yrs), were studied electron microscopically. Ultrastructurally most of the melanin granules in the mature female liver showed an internal structure similar to the melanin granules of the oocytes. The hepatic melanin granules of immature females a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220293
更新日期：1977-12-19 00:00:00
abstract：:Colonies of the ascidian Botryllus schlosseri undergo recurrent generation changes in which adult zooids are gradually resorbed and replaced by new blastogenic generations. During these periods, known as take-over phases, programmed cell death, which, on the basis of morphological analysis is ascribed to apoptosis, oc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0738-9
更新日期：2003-06-01 00:00:00
abstract：:The therapeutic potency of delivered mesenchymal stem cells (MSCs) in tissue engineering applications may be improved by priming cells toward a differentiated state via coculture with native, differentiated cells prior to implantation; however, there is a lack of understanding in what may be the most efficacious metho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1265-8
更新日期：2012-03-01 00:00:00
abstract：:The innervation pattern of distal muscle fibers of the opener muscle of walking legs of crayfish (Astacus leptodactylus) was investigated using methylene-blue staining, cobalt infiltration, and electron microscopy. A quantitative analysis of the entire innervation of single muscle fibers was attempted. It was found th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213750
更新日期：1982-01-01 00:00:00
abstract：:Four different types of spermatogonia were identified in the seminiferous tubules of the Japanese quail: a dark type A (Ad), 2 pale A types (Ap1 and Ap2), and a type B. A model is proposed describing the process of spermatogonial development in the quail. The Ad spermatogonia are considered to be the stem cells. Each ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319382
更新日期：1992-03-01 00:00:00
abstract：:The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223268
更新日期：1975-09-16 00:00:00
abstract：:Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000262
更新日期：2001-05-01 00:00:00
abstract：:Digital dermatitis (DD), a common ulcerative disease of the bovine foot causing lameness and reducing productivity and animal welfare, is associated with infection by spirochete Treponema bacteria. Topical tetracycline, the most common treatment, has inconsistent cure rates; therefore, new therapeutic options are need...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03082-y
更新日期：2020-02-01 00:00:00
abstract：:Recent evidence indicates that many molecules involved in generating and patterning the limbs also play a role during craniofacial morphogenesis. On the surface, this is an unexpected finding given that these regions of the body have separate evolutionary origins, are composed of different embryonic tissues, and are q...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051271
更新日期：1999-04-01 00:00:00
abstract：:Previous immunochemical investigations have demonstrated various opioid peptides in the pancreas. However, controversies exist related to the cellular localization of these peptides in the endocrine pancreas. Therefore, the guinea pig endocrine pancreas was immunohistochemically investigated for the presence of opioid...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318454
更新日期：1990-02-01 00:00:00
abstract：:In the chironomid Acricotopus lucidus, germ line-soma differentiation becomes evident with the formation of the pole cells and the elimination of the germ line-limited chromosomes (Ks) from the future somatic nuclei of the embryo. Unlike in Drosophila, the early nuclear divisions do not proceed synchronously in A. luc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000270
更新日期：2000-11-01 00:00:00
abstract：:The suprachiasmatic nucleus (SCN) contains a biological clock that generates timing signals that drive daily rhythms in behaviors and homeostatic functions. In addition to this pacemaker function, the SCN gates its own sensitivity to incoming signals, which permits appropriate temporal adjustment to achieve synchrony ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-002-0576-1
更新日期：2002-07-01 00:00:00
abstract：:Glutamate is the major excitatory neurotransmitter of the mammalian retina and glutamate uptake is essential for normal transmission at glutamatergic synapses. The reverse transcriptase-polymerase chain reaction (RT-PCR) has revealed the presence of three different high-affinity glutamate transporters in the rat retin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050976
更新日期：1998-01-01 00:00:00
abstract：:The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized R...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050575
更新日期：1996-04-01 00:00:00
abstract：:Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were im...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2614-z
更新日期：2017-08-01 00:00:00
abstract：:In an attempt to determine the neural control of pancreatic D cells, the pancreatic islets of the domestic fowl were examined electron microscopically from 1 to 28 days after abdominal vagotomy. Exocytotic release of many secretory granules from D cells occurred one day after vagotomy. Rough endoplasmic reticulum deve...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233732
更新日期：1980-01-01 00:00:00
abstract：:The effect of hypothyroidism, induced by 4-propyl-2-thiouracil, on muscle satellite cells in vivo and in vitro, and on postnatal muscle fiber development in the soleus muscle of rats during the first 40 days of postnatal life was analyzed. The proliferative activity of satellite cells was determined by means of bromod...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050682
更新日期：1996-10-01 00:00:00
abstract：:The ontogeny of two gonadotropin-releasing-hormone (GnRH) systems, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), was investigated in zebrafish (Danio rerio). In situ hybridization (ISH) first detected sGnRH mRNA-expressing cells at 1 day post-fertilization (pf) anterior to the developing olfactory organs. Subseq...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0279-0
更新日期：2007-02-01 00:00:00
abstract：:In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1777-5
更新日期：2014-04-01 00:00:00
abstract：:MN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acros...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00645047
更新日期：1992-12-01 00:00:00
abstract：:Bone marrow (BM) from human and rodent species contains a population of multipotential cells referred to as mesenchymal stem cells (MSCs). Currently, MSCs are isolated indirectly by using a culture step and then the generation of fibroblast colony-forming units (CFU-fs). Unprocessed or native BM MSCs have not yet been...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0292-3
更新日期：2007-03-01 00:00:00
abstract：:The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233914
更新日期：1979-03-19 00:00:00
abstract：:The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1092-x
更新日期：2005-06-01 00:00:00
abstract：:Adult white-footed mice, Peromyscus leucopus, were kept either in long photoperiod (LP; LD 16:8) or in short photoperiod (SP; LD 8:16) for six weeks. Ovaries were serially sectioned and examined histologically. Preantral follicles were observed to release their oocyte into the ovarian stroma. This phenomenon is termed...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218373
更新日期：1982-01-01 00:00:00
abstract：:Post-translational modification of histone proteins, such as the methylation of lysine and arginine residues, influences the higher order of chromatin and leads to gene activation or silencing. Histone methyltransferases or demethylases actively add or remove various methylation marks in a cell-type-specific and conte...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1842-8
更新日期：2014-06-01 00:00:00
abstract：:This work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. The locust ovipositor muscle is the only insect system in which more than 100 tension receptor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0498-z
更新日期：2007-12-01 00:00:00
abstract：:The retina of the phalangid, Opilio ravennae, consists of retinula cells with distal rhabdomeres, arhabdomeric cells, and sheath cells. The receptive segment of retinula cells shows a clear separation into a proximal rhabdom, organized into distinct rhabdom units formed by three or four retinula cells, and a distal rh...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233658
更新日期：1979-01-01 00:00:00
abstract：:The effects of high dose injections of corticotropin-releasing hormone (CRH) on the adrenal cortex of hypophysectomized rats were studied at the light- and electron-microscopical levels. Adrenocortical atrophy induced by hypophysectomy could be reduced by daily i.p. injection of 10 micrograms (3 nmol) CRH given for 3 ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297501
更新日期：1990-04-01 00:00:00
abstract：:In the central nervous system, serotonergic and dopaminergic signaling is terminated by the activity of specialized transporter proteins for serotonin (SERT) and dopamine (DAT). These transporter proteins are found both on the cell surface and in intracellular transport vesicles. Trafficking between these subcellular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1256-9
更新日期：2011-11-01 00:00:00
