Primary culture of antennal mechanoreceptor neurons of Manduca sexta.

Abstract:

:We have developed a primary cell culture system of antennal mechanoreceptor neurons from early-stage pupal sphinx moth Manduca sexta. Dissociated neurons from the moth antennae differentiated, grew and survived for several weeks in a conditioned culture medium. Bipolar neurons with soma diameters of 10-25 microns from the basal portion of the antennae could be positively identified as mechanoreceptor neurons, presumably derived from Johnston's organ, using a monoclonal antibody that recognizes neurofilaments in these neurons. The immunoreactivity was clear and specific from the first day after dissociation and became stronger during several days in culture. These neurons appeared healthy and showed normal whole-cell properties only a few days after plating. We found numerous mechanosensitive ion channels responding to both negative and positive pressures on the somata and neurites of differentiated neurons. This new culture system provides access to mechanoreceptor neurons that has never been possible before, allowing the use of both mechanical and electrical stimuli on neurons that are free from the accessory structures surrounding them in intact preparations.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Torkkeli PH,French AS

doi

10.1007/s004410051358

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

301-9

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

297

pub_type

杂志文章
  • Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.

    abstract::Experimental autoimmune orchitis (EAO) in the rat is the primary chronic animal model for the investigation of one of the main causes of male infertility, viz., testicular inflammation. Dendritic cells (DC) are potent antigen-presenting cells that play a fundamental role in autoimmune disease. We investigated the numb...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-0129-5

    authors: Rival C,Lustig L,Iosub R,Guazzone VA,Schneider E,Meinhardt A,Fijak M

    更新日期:2006-05-01 00:00:00

  • Electron microscopic and histochemical investigations of the atretic oocyte of Perca fluviatilis L. (Teleostei).

    abstract::The regression of atretic oocytes in Perca fluviatilis was studied by histochemical, light and electron microscopic methods. The course of regression can be divided into three stages, the first two comprising the dissolution of the atretic oocyte and its phagocytosis by the granulosa cells of the follicular epithelium...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00209978

    authors: Lang I

    更新日期:1981-01-01 00:00:00

  • FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle.

    abstract::Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-017-2614-z

    authors: Baptista IL,Silvestre JG,Silva WJ,Labeit S,Moriscot AS

    更新日期:2017-08-01 00:00:00

  • Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris.

    abstract::The distribution of FMRFamide-like immunoreactive cell bodies and fibers in the nervous system of the earthworm Lumbricus terrestris has been studied by means of immunocytochemistry. The cerebral ganglion contains 150-170 immunoreactive nerve cells that are organized into six major groups in the rostral and five major...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050843

    authors: Reglödi D,Slezák S,Lubics A,Szelier M,Elekes K,Lengvári I

    更新日期:1997-06-01 00:00:00

  • The peripheral innervation of the gill of the marine mollusc demonstrated by the aluminium-formaldehyde (ALFA) histofluorescence method.

    abstract::The aluminium-formaldehyde (ALFA) histofluorescence method was used to study the innervation of the gill of the marine bivalve mollusc Mytilus edulis and the results were contrasted with those obtained with the standard formaldehyde-induced-fluorescence (FIF) method. The ALFA method produced more fluorescing structure...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214696

    authors: Catapane EJ

    更新日期:1982-01-01 00:00:00

  • Evidence that two forms of choline acetyltransferase are differentially expressed in subclasses of enteric neurons.

    abstract::Cholinergic neurons have been revealed in the enteric nervous system by functional and biochemical studies but not by antibodies that provide excellent localisation of the synthesising enzyme, choline acetyltransferase (ChAT), in the central nervous system. In order to determine whether a newly described peripheral fo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-002-0652-6

    authors: Chiocchetti R,Poole DP,Kimura H,Aimi Y,Robbins HL,Castelucci P,Furness JB

    更新日期:2003-01-01 00:00:00

  • Ultrastructural changes in the spiral limbus associated with carboplatin-induced ablation of inner hair cells.

    abstract::Four months after the selective ablation of inner hair cells by carboplatin, the interdental cell epithelium exhibited dilated intercellular spaces and cytosolic vacuoles not seen in controls. In addition, the wide, often electron-lucent phalanges observed in the interdental cells of the normal chinchilla collapsed in...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000253

    authors: Spicer SS,Salvi RJ,Schulte BA

    更新日期:2000-10-01 00:00:00

  • Presence and localization of antithrombin and its regulation after acute lipopolysaccharide exposure in amphioxus, with implications for the origin of vertebrate liver.

    abstract::Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-005-0088-x

    authors: Liang Y,Zhang S,Lun L,Han L

    更新日期:2006-03-01 00:00:00

  • The carbohydrates of secretory granules and the glycocalyx in developing mucoid cells.

    abstract::Complex carbohydrates in secretory granules and at the apical cell surface of mouse gastric mucoid cells were studied during embryogenesis and in the early postnatal period by various cytochemical methods; the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) and tannic acid-uranyl acetate (TA-UA) procedu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214559

    authors: Pipan N,Psenicnik M

    更新日期:1985-01-01 00:00:00

  • Incorporation of L-(3H)-fucose into glycoproteins of the adrenal gland of mice. Light microscope radioautographic study on semi-thin sections.

    abstract::To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00232246

    authors: Haddad A,Brasileiro IL,Pelletier G

    更新日期:1979-11-01 00:00:00

  • The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa.

    abstract::The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-001-0499-2

    authors: Schürmann A,Axer H,Scheepers A,Doege H,Joost HG

    更新日期:2002-02-01 00:00:00

  • The distribution of GABA and glycine immunostaining in the cochlear nucleus of the mustached bat (Pteronotus parnellii).

    abstract::The distribution of neuronal elements immunoreactive for gamma-aminobutyric acid (GABA) and glycine in the cochlear nucleus of the mustached bat Pteronotus parnellii has been studied by means of the postembedding technique on serial semithin sections. Our goal has been to identify similarities and differences in the o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050773

    authors: Kemmer M,Vater M

    更新日期:1997-02-01 00:00:00

  • Midbrain-derived neural stem cells: from basic science to therapeutic approaches.

    abstract::Neural stem cells (NSCs) are a subtype of tissue-specific progenitor cells capable of extended self-renewal and the ability to generate all major cell types of nervous tissue, such as neurons, astroglia and oligodendroglial cells. Recent studies suggest that salient patterning in anterior-posterior and dorsal-ventral ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-004-0923-5

    authors: Storch A,Sabolek M,Milosevic J,Schwarz SC,Schwarz J

    更新日期:2004-10-01 00:00:00

  • Some evidence for the ampullary organs in the European cave salamander Proteus anguinus (Urodela, Amphibia).

    abstract::The multicellular epithelial organs in Proteus anguinus, which Bugnion (1873) assumed to be developing neuromasts, have been analyzed by light- and electron-microscopy. Their fundamental structure consists of single ampullae with sensory and accessory cells with apical parts that extend into the pit of the ampulla, an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00217865

    authors: Istenic L,Bulog B

    更新日期:1984-01-01 00:00:00

  • Neuromedin U-immunoreactivity in the nervous system of the small intestine of the pig and its coexistence with substance P and CGRP.

    abstract::In the small intestine of the pig, neuromedin U (NMU)-immunoreactivity was mainly confined to the nerve plexus of the inner submucosal and mucosal regions. After colchicine treatment, a high number of immunoreactive nerve cell bodies was observed in the plexus submucosus internus (Meissner), whereas only a low number ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00239453

    authors: Timmermans JP,Scheuermann DW,Stach W,Adriaensen D,De Groodt-Lasseel MH,Polak JM

    更新日期:1989-11-01 00:00:00

  • Endogenous tissue engineering: PTH therapy for skeletal repair.

    abstract::Based on its proven anabolic effects on bone in osteoporosis patients, recombinant parathyroid hormone (PTH(1-34)) has been evaluated as a potential therapy for skeletal repair. In animals, the effect of PTH(1-34) has been investigated in various skeletal repair models such as fractures, allografting, spinal arthrodes...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-011-1188-4

    authors: Takahata M,Awad HA,O'Keefe RJ,Bukata SV,Schwarz EM

    更新日期:2012-03-01 00:00:00

  • The chemoreceptor surface of the taste organ in the frog Rana esculenta. A freeze-fracture analysis.

    abstract::The epithelial surface of the taste disc on the fungiform papillae of the frog was studied by means of freeze-fracture and conventional electron microscopy. The plasma membrane of the apical process of taste receptor cells was found to exhibit a uniform population of 10-nm intramembrane particles on its fracture face ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216883

    authors: Röhlich P,Pevzner RA

    更新日期:1982-01-01 00:00:00

  • Components and connections of the circadian timing system in mammals.

    abstract::The circadian timing system is a neural network consisting of the hypothalamic suprachiasmatic nucleus, aided by the retina, other hypothalamic nuclei, autonomic regions of the spinal cord, sympathetic ganglia and the pineal gland. Extensive studies conducted over the last two decades have unravelled the principal ite...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s004410050652

    authors: Reuss S

    更新日期:1996-09-01 00:00:00

  • Effects of an antiandrogen, cyproterone acetate, on the kidney of the three-spined stickleback (Gasterosteus aculeatus L.).

    abstract::The fine structure of the kidney is studied in the sexually mature male three-spined stickleback after administration of an antiandrogen, cyproterone acetate. Under these conditions, the dedifferentiation of renal tubules is characterized by the same involutive processes as those induced by castration, with the differ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00220324

    authors: Mourier JP

    更新日期:1976-10-13 00:00:00

  • Comparative brain architecture of the European shore crab Carcinus maenas (Brachyura) and the common hermit crab Pagurus bernhardus (Anomura) with notes on other marine hermit crabs.

    abstract::The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-012-1353-4

    authors: Krieger J,Sombke A,Seefluth F,Kenning M,Hansson BS,Harzsch S

    更新日期:2012-04-01 00:00:00

  • Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein.

    abstract::Prolonged secretory stimulation of the exocrine pancreas in the rat by in vivo infusion of caerulein leads to a rapid degranulation of the organ associated with a progressive reduction in the size of the zymogen granules. During the first six to twelve hours of stimulation Golgi complexes are enlarged and several stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00224474

    authors: Bieger W,Martin-Achard A,Bassler M,Kern HF

    更新日期:1976-01-28 00:00:00

  • Phagocytosis in cellular defense and nutrition: a food-centered approach to the evolution of macrophages.

    abstract::The uptake of macromolecules and larger energy-rich particles into the cell is known as phagocytosis. Phagocytosed material is enzymatically degraded in membrane-bound vesicles of the endosome/lysosome system (intracellular digestion). Whereas most, if not all, cells of the animal body are equipped with the molecular ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-019-03096-6

    authors: Hartenstein V,Martinez P

    更新日期:2019-09-01 00:00:00

  • Long-term staining of live Merkel cells with FM dyes.

    abstract::Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-002-0688-7

    authors: Fukuda J,Ishimine H,Masaki Y

    更新日期:2003-03-01 00:00:00

  • Neuropeptide Y: intrapancreatic neuronal localization and effects on insulin secretion in the mouse.

    abstract::The intrapancreatic localization and the effects on basal and stimulated insulin secretion of neuropeptide Y (NPY) were investigated in the mouse. Immunocytochemistry showed NPY to be confined to intrapancreatic nerve fibers mainly associated with blood vessels. Fine varicose NPY fibers were also detected in the exocr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF01239960

    authors: Pettersson M,Ahrén B,Lundquist I,Böttcher G,Sundler F

    更新日期:1987-04-01 00:00:00

  • A study of scanning (SEM) and transmission (TEM) electron microscopy of the glomerular capillaries in developing rat kidney.

    abstract::Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233959

    authors: Kazimierczak J

    更新日期:1980-01-01 00:00:00

  • Lithium alleviated spinal cord injury (SCI)-induced apoptosis and inflammation in rats via BDNF-AS/miR-9-5p axis.

    abstract::Spinal cord injury (SCI) is a major cause of paralysis, disability and even death in severe cases. Lithium has neuroprotective effects on SCI, while the underlying mechanisms remain obscure. In the present study, we established a SCI rat model, which subsequently received lithium treatment. Results displayed that lith...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-020-03298-3

    authors: Wang F,Chang S,Li J,Wang D,Li H,He X

    更新日期:2021-01-19 00:00:00

  • Immunolocalization of inhibin alpha-subunit in the human testis. A light- and electron-microscopy study.

    abstract::The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00319612

    authors: Vannelli GB,Barni T,Forti G,Negro-Vilar A,Vale W,Serio M,Balboni GC

    更新日期:1992-08-01 00:00:00

  • Isolation and characterization of cluster of differentiation 9-positive ependymal cells as potential adult neural stem/progenitor cells in the third ventricle of adult rats.

    abstract::Ependymal cells located above the ventricular zone of the lateral, third, and fourth ventricles and the spinal cord are thought to form part of the adult neurogenic niche. Many studies have focused on ependymal cells as potential adult neural stem/progenitor cells. To investigate the functions of ependymal cells, a si...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-019-03132-5

    authors: Horiguchi K,Yoshida S,Hasegawa R,Takigami S,Ohsako S,Kato T,Kato Y

    更新日期:2020-03-01 00:00:00

  • Cardiac dysfunction in transgenic mouse fetuses overexpressing shortened type XIII collagen.

    abstract::Overexpression of type XIII collagen molecules with an 83-amino-acid residue in-frame deletion of part of the ectodomain leads to fetal lethality in Col13a1COL2del transgenic mice. We characterize here the functional disturbances in the cardiovascular system of mouse fetuses overexpressing mutant type XIII collagen. D...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-008-0617-5

    authors: Tahkola J,Räsänen J,Sund M,Mäkikallio K,Autio-Harmainen H,Pihlajaniemi T

    更新日期:2008-07-01 00:00:00

  • Development of adrenal chromaffin cells in Sf1 heterozygous mice.

    abstract::Adrenal medullary chromaffin cells are derivatives of the neural crest and are widely believed to share a common sympathoadrenal (SA) progenitor with sympathetic neurons. For decades, the adrenal cortical environment was assumed to be essential for channelling SA progenitors towards an endocrine chromaffin cell fate. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-006-0213-5

    authors: Lohr J,Gut P,Karch N,Unsicker K,Huber K

    更新日期:2006-09-01 00:00:00