Abstract：
:Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in tissue culture. To find dyes that remain in the cells for a long period of time, we tested many fluorescence dyes and found that FM dyes (such as FM1-43) are useful markers for live Merkel cells. In the rat footpad skin, FM1-43 was shown to stain 95% of live Merkel cells that were already stained with quinacrine. FM4-64 stained 98% of quinacrine-stained Merkel cells. Merkel cells in sinus hair follicles were also stained with FM dyes. The fluorescence intensity of FM dyes was stronger than that of quinacrine, and the shape of the cells was more distinct in the FM-dye-stained cells. To test how long FM dyes remain in live cells, FM-dye-stained Merkel cells in hair follicles were embedded in collagen gel and were cultured in a serum-free medium. FM-dye-stained cells were easily identified even after 7 days of culture. During the culture, Merkel cells changed their shape, moved in the preparation and tended to aggregate on the surface. We conclude that FM dyes are powerful tools for tracing live Merkel cells in in vitro experiments. Moreover, the finding that Merkel cells incorporate FM dyes suggests that vesicles in the cells are likely to have mechanisms of recycling in a manner similar to those in neurons and secretory cells.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Fukuda J,Ishimine H,Masaki Ydoi
10.1007/s00441-002-0688-7keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
325-32issue
3eissn
0302-766Xissn
1432-0878journal_volume
311pub_type
杂志文章abstract：:The suprachiasmatic nucleus (SCN) contains a biological clock that generates timing signals that drive daily rhythms in behaviors and homeostatic functions. In addition to this pacemaker function, the SCN gates its own sensitivity to incoming signals, which permits appropriate temporal adjustment to achieve synchrony ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-002-0576-1
更新日期：2002-07-01 00:00:00
abstract：:A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050800
更新日期：1997-04-01 00:00:00
abstract：:The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time period...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234394
更新日期：1980-01-01 00:00:00
abstract：:Guanosine triphosphatase (GTPase) activity was studied histo- and cytochemically in the rod outer segments of the rat retina by means of a newly developed method. Differences in the distribution pattern of the enzyme activity exist within the outer segment: the activity is more intense at the tip of the rod outer segm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217416
更新日期：1983-01-01 00:00:00
abstract：:The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5) in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical locali...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217869
更新日期：1984-01-01 00:00:00
abstract：:The problem of the blood-brain barrier in the pineal organ of the rainbow trout, Salmo gairdneri, was investigated following intraperitoneal or intracardial injections of several tracers and dyes with different molecular weights. As demonstrated at the light-microscopic level, repeated injections of trypan blue or hor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219238
更新日期：1985-01-01 00:00:00
abstract：:iASPP (inhibitory member of the ASPP family), the conserved key inhibitor of p53, is overexpressed and plays an important oncogenetic role in many human cancers. However, its function in cervical cancer remains unknown. The objective of this study was to investigate the expression and clinical relevance of iASPP in ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1569-y
更新日期：2013-05-01 00:00:00
abstract：:Retinae of Abyssinian cats homozygous for a retinal degeneration gene, and normal controls, have been investigated using antibodies directed against opsin, transducin alpha (TD-alpha), S-antigen (48K protein), interphotoreceptor retinoid-binding protein (IRBP), and cone outer segments. IRBP-immunoreactivity (IR) is mu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221474
更新日期：1989-09-01 00:00:00
abstract：:With the Bodian method stained fibers were observed in the lobules of the rat liver and with the modified Karnovsky and Roots thiocholine method cholinesterase (presumably acetylcholinesterase (AChE))-positive nerve fibers were found in a pattern similar to that of the Bodian-stained fibers. The AChE-positive nerve fi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219403
更新日期：1976-08-20 00:00:00
abstract：:The maternal-fetal interface, a chimeric structure, is formed when fetal cytotrophoblasts (CTBs) from the placenta invade the uterine wall and its resident vasculature. In preeclampsia (PE), interstitial and endovascular invasion are often shallow, and fewer spiral arterioles are breached in toto. Our previous work ha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0428-0
更新日期：2007-09-01 00:00:00
abstract：:The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221498
更新日期：1982-01-01 00:00:00
abstract：:An electron microscopical investigation of the pineal gland of the adult noctule bat revealed the presence of some peculiar ciliary derivatives, similar to the club-shaped outer segment of rudimentary photoreceptor cells in the pineal organ of nonmammalian vertebrates. The pinealocytes of population I can be classifie...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222058
更新日期：1977-07-26 00:00:00
abstract：:The presence, location and degree of immunoexpression of various microfilament (MF) and intermediate filament (IF) systems (actin, cytokeratins, desmin, vimentin) were studied in the excurrent ducts of the testis in sexually mature and active galliform (Japanese quail, domestic fowl, turkey) and anseriform (duck) bird...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0631-7
更新日期：2008-08-01 00:00:00
abstract：:We used immunohistochemistry to identify cells present in pituitary allografts in the hamster. Hypophyses removed from neonatal hamsters or adenohypophyses removed from adult females were placed beneath renal capsules of hypophysectomized adult females. Serum PRL, LH, and GH concentrations were measured at two, five a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215467
更新日期：1988-01-01 00:00:00
abstract：:The spatial localisation of insulin-like growth-factor-binding protein-2 (IGFBP-2) and its mRNA was investigated during larval and post-larval developmental stages of the gilthead seabream ( Sparus aurata) by immunohistochemistry and in situ hybridisation with specific antisera and riboprobes. During larval developmen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0952-0
更新日期：2005-01-01 00:00:00
abstract：:In this study, we focus on the specific contribution of β integrin-like protein to adhesion-mediated events in molluscan larval cells in culture that could not have been investigated within the whole animal. An analysis of disturbances to cell-substratum adhesion, caused by the integrin receptor inhibiting Arg-Gly-Asp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2122-y
更新日期：2015-08-01 00:00:00
abstract：:Triad density relative to sarcomeres, size of T-system tubules, sarcomere length, muscle fiber diameter in native and fixed states, and size of myofibrils were measured in four straited muscle fiber types in Atlantic hagfishes (Myxine glutinosa, L.) of different sizes. Traids occur at A/I-injunctions in all fiber type...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223227
更新日期：1975-01-01 00:00:00
abstract：:The fine structure of the kidney is studied in the sexually mature male three-spined stickleback after administration of an antiandrogen, cyproterone acetate. Under these conditions, the dedifferentiation of renal tubules is characterized by the same involutive processes as those induced by castration, with the differ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220324
更新日期：1976-10-13 00:00:00
abstract：:The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219054
更新日期：1977-03-09 00:00:00
abstract：:Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparatio...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217847
更新日期：1984-01-01 00:00:00
abstract：:The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and rese...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220316
更新日期：1976-10-13 00:00:00
abstract：:Transmission electron microscopy was used to study the effects of proteolytic enzymes (collagenase, trypsin, clostripain), the calcium chelator ethyleneglycol-bis-(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA), and the calcium ionophore A 23187 on substrate adhesion and fine structure of chondrocytes and fi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00228443
更新日期：1984-01-01 00:00:00
abstract：:We chose synaptic terminals of rat rod bipolar cells as a model system to study activity-related changes in the overall morphology and the fine structure of synaptic sites. Using confocal laser scanning microscopy in conjunction with three-dimensional reconstruction and electron microscopy, we examined the effect of l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051174
更新日期：1998-11-01 00:00:00
abstract：:Endothelins (ET) are a family of vasoactive peptides that act via two subtypes of receptors, named ETA and ETB. ET-1 binds to both ETA and ETB, whereas the isopeptide ET-3 preferentially binds to ETB. The localization of ETA and ETB receptors in the rat adrenal gland and their involvement in the adrenal secretagogue e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050820
更新日期：1997-05-01 00:00:00
abstract：:Pulmonary neuroepithelial bodies (NEBs) are extensively innervated organoid groups of neuroendocrine cells that lie in the epithelium of intrapulmonary airways. Our present understanding of the morphology of NEBs is comprehensive, but direct physiological studies have so far been challenging because the extremely diff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1111-y
更新日期：2005-07-01 00:00:00
abstract：:Three sites of somatostatin-synthesizing perikarya, or a related antigen, were determined by immunofluorescence in the hypothalamus of the tadpole, Alytes obstetricans (Amphibia, Anura). Two sites of neurosecretory perikarya were localized in the preoptic nuclei of the anterior hypothalamus; the axons extended either ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224373
更新日期：1978-02-24 00:00:00
abstract：:Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual beha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1394-8
更新日期：2012-06-01 00:00:00
abstract：:The number, size and structure of tendon organs were examined in leg muscles of the rat 3-19 weeks after de-efferentation performed in newborn animals by removal of the lumbosacral spinal cord. After this operation, tendon organs differentiated and grew in disused muscles and were innervated by primary sensory neurons...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214645
更新日期：1985-01-01 00:00:00
abstract：:Rats were injected with liposomes containing iodixanol (CTP10 Injection; 100 mg iodine per kg body weight) followed by a second injection of 125I-tyramine-cellobiose-albumin microspheres. The amounts of phagocytosed and degraded labelled albumin in liver were measured. A reduced uptake and degradation of albumin micro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000229
更新日期：2000-06-01 00:00:00
abstract：:The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237730
更新日期：1979-06-08 00:00:00
