Abstract：
:Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual behaviour, arousal, and hypothalamic/hypophyseal axis, these neuropeptides may exert a local action in various peripheral organs and tissues. Emerging evidence suggests a main role of OxA and its highly specific receptor Ox1R in the male genital tract of mammals. We previously demonstrated OxA localization in Sertoli cells and spermatids of rat testis. Here, we show positive stainings of Ox1R in developing spermatocytes, and spermatids of rat testis by immunohistochemistry. The expression of Ox1R mRNA and the protein in the tissue was also established by reverse-transcription polymerase chain reaction and Western blotting respectively. The addition of OxA to fresh testis slices significantly increased testosterone (T) secretion which, conversely, was inhibited by Mullerian inhibiting substance (MIS). The sequential treatment of testis samples with the two substances highlighted an antagonizing activity of OxA versus MIS in regulating T levels. Furthermore, the stimulating effect on T production by OxA was prevented by the addition of the selective Ox1R inhibitor SB-408124. Overall, our findings suggest that locally secreted OxA interacting with Ox1R activates signals which antagonize MIS action in the control of T levels in mammalian testis.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Assisi L,Tafuri S,Liguori G,Paino S,Pavone LM,Staiano N,Vittoria Adoi
10.1007/s00441-012-1394-8subject
Has Abstractpub_date
2012-06-01 00:00:00pages
601-7issue
3eissn
0302-766Xissn
1432-0878journal_volume
348pub_type
杂志文章abstract：:The transient receptor potential (TRP) superfamily comprises a group of non-selective cation channels that have been implicated in both receptor and store-operated channel functions. The family of the classical TRPs (TRPCs) consists of seven members (TRPC1-7). The presence of TRPC1 and TRPC5 mRNA in the brain has prev...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0004-4
更新日期：2005-11-01 00:00:00
abstract：:Intracellular insulin-binding sites were directly traced in fixed monolayer cultures of a variety of cell types with the use of two fluorescent derivatives of insulin, viz. fluorescein isothiocyanate (FITC)-labelled and tetramethyl rhodamine isothiocyanate (TMRITC)-labelled insulin. Both derivatives retained the prope...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232150
更新日期：1980-01-01 00:00:00
abstract：:The secretory activity in the subcommissural organ (SCO) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. Among the various lectins tested. Concanavalin A (Con A) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222295
更新日期：1988-08-01 00:00:00
abstract：:A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03241-6
更新日期：2020-11-01 00:00:00
abstract：:By use of the immunofluorescence technique TSH-, LH- and ACTH-cells were localized in the hypophysis of tadpoles of Xenopus laevis. The first signs of the activity of these cells were observed in early stages of the development, i.e., stage 39 for ACTH, and stage 42 for TSH and LH. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216218
更新日期：1982-01-01 00:00:00
abstract：:Osteoclasts are highly polarized cells from both morphological and functional points of view. Using quick-freeze, rotary-replication methods combined with cell-shearing, we clarified the variability of cytoplasmic surface of the polarized membranes of osteoclasts seeded on apatite. As to the organization of actin fila...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2085-4
更新日期：2015-05-01 00:00:00
abstract：:Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was m...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220036
更新日期：1988-10-01 00:00:00
abstract：:Complex carbohydrates in secretory granules and at the apical cell surface of mouse gastric mucoid cells were studied during embryogenesis and in the early postnatal period by various cytochemical methods; the periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) and tannic acid-uranyl acetate (TA-UA) procedu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214559
更新日期：1985-01-01 00:00:00
abstract：:The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1077-9
更新日期：2005-05-01 00:00:00
abstract：:Growing numbers of translational genomics studies are based on the highly efficient and versatile zebrafish (Danio rerio) vertebrate model. The increasing types of zebrafish models have improved our understanding of inherited kidney diseases, since they not only display pathophysiological changes but also give us the ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-017-2593-0
更新日期：2017-07-01 00:00:00
abstract：:The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals' intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric plexus (MP). This study quantifies enteric neurons in the ISP, OSP, and MP of the pig ascending (AC) and descending colon (DC) using the HuC/D,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03286-7
更新日期：2020-09-23 00:00:00
abstract：:A recently developed immunohistochemical technique for serotonin (Steinbusch et al. 1978) was used in the present investigation to study the occurrence of this indoleamine in the granulated epithelial cells of neuro-epithelial bodies (NEB). Lungs from neonatal rabbits and pigs exhibit immunoreactive cell groups identi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217095
更新日期：1982-01-01 00:00:00
abstract：:The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218204
更新日期：1987-05-01 00:00:00
abstract：:An increasingly pro-oxidant environment has been widely implicated in causing dysfunction of testicular steroidogenesis, but little progress has been made in understanding the underlying molecular mechanism. Here, we report that gamma-glutamyl transferase 5 (GGT5), a key metabolism component responsible for the cataly...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2458-y
更新日期：2016-11-01 00:00:00
abstract：:In the central nervous system, serotonergic and dopaminergic signaling is terminated by the activity of specialized transporter proteins for serotonin (SERT) and dopamine (DAT). These transporter proteins are found both on the cell surface and in intracellular transport vesicles. Trafficking between these subcellular ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1256-9
更新日期：2011-11-01 00:00:00
abstract：:The vertebrate limb is a powerful model system for studying the cellular and molecular interactions that determine morphological pattern during embryonic development. Recent advances in our understanding of these interactions have shed new light on the molecular mechanisms of vertebrate limb development, evolution and...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051261
更新日期：1999-04-01 00:00:00
abstract：:Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug. In the distal elongating tips of myotubes, microtubules were the major cytoskeletal el...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214368
更新日期：1988-05-01 00:00:00
abstract：:Macrophages are present throughout the anterior pituitary gland. However, the features and function of macrophages in the gland are poorly understood. Recent studies have indicated that there are two main macrophage classes: M1 (classically activated) and M2 (alternatively activated). In this study, we examine whether...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2564-x
更新日期：2017-05-01 00:00:00
abstract：:Hydroxyindole O-methyltransferase (HIOMT)-immunoreactive cells and melatonin synthesis were demonstrated in bovine epithalamus (including the pineal gland) with monoclonal antibodies and cRNA probes to HIOMT. The HIOMT-immunoreactive product was present in the cytoplasm of pinealocytes. All identifiable pinealocytes w...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327275
更新日期：1991-03-01 00:00:00
abstract：:Seasonal changes have been detected in the ultrastructure of brown adipose tissue (BAT) of the common shrew, Sorex araneus (a true nonhibernator) living under natural conditions and collected at the time when the most representative growth phase of the animal for the given season could be expected. In summer and autum...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233501
更新日期：1981-01-01 00:00:00
abstract：:The expression patterns of three microtubule-associated proteins (MAP1A, MAP1B, and MAP2A&B) were investigated in the developing optic tectum. Expression of MAP1B and middle-molecular-weight peptide of neurofilament (NF-M) was first observed in the same mesencephalic cells on day 3 of incubation, indicating that neuro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0796-z
更新日期：2003-12-01 00:00:00
abstract：:Pancreatic acinar cells of euthermic, hibernating and arousing individuals of the hazel dormouse Muscardinus avellanarius (Gliridae) have been observed at the electron-microscopic level and analysed by means of ultrastructural morphometry and immunocytochemistry in order to investigate possible fine structural changes...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051082
更新日期：1998-06-01 00:00:00
abstract：:Nitric oxide (NO) stimulates osteoblast differentiation, but whether NO contributes to odontoblast differentiation during dentin repair is unknown. By using reverse transcription/polymerase chain reaction and immunostaining, we investigated the gene expression and/or immunolocalization of endothelial NO synthase (eNOS...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0003-5
更新日期：2007-04-01 00:00:00
abstract：:The innervation of the anococcygeus muscle of the rat was investigated with regard to the histochemical features of nerve fibers within the muscle and to the location of the postganglionic autonomic neurons which are the source of these fibers. Acetylcholinesterase-positive fibers and catecholaminergic fibers are abun...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00571438
更新日期：1990-01-01 00:00:00
abstract：:Neurosecretory pathways were examined in normal male rats by the use of the immunoperoxidase technique for the localization of neurophysin in Bouin-fixed, deparaffinized sections. Using this technique two projections of extrahypothalamic neuorosecretory fibers can be traced to the sites of origin of the choid plexuses...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232829
更新日期：1977-03-01 00:00:00
abstract：:Transplantation of ligament-tissue-derived stem cells has become a promising approach in the repair of injured ligament. Neovascularization plays an important role in ligament healing and remodeling. Recently, human umbilical-cord-blood-derived CD34+ cells have been reported to contribute to neoangiogenesis. Therefore...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2250-4
更新日期：2015-12-01 00:00:00
abstract：:In the rostral hypothalamus of the domestic fowl, the magnocellular neurosecretory nuclei show a peculiar differentiation. Golgi studies of the supraoptic and paraventricular nuclei of the fowl reveal at least two major cell types: 1) large multipolar neurons, and 2) small interneurons. Golgi impregnations provide a d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236253
更新日期：1981-01-01 00:00:00
abstract：:Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0088-x
更新日期：2006-03-01 00:00:00
abstract：:Cobaltous-lysine is transported anterogradely from the optic nerve of the teleost, Lethrinus chrysostomus (Lethrinidae, Perciformes). The marginal optic tract is labelled in longitudinal bands of light and dark staining fibres which persists caudally within the ventral division but not in the dorsal division. This spe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218889
更新日期：1989-01-01 00:00:00
abstract：:With the gallocyanine technique (Wittkowski, Bock and Franken, 1970) Gomori-positive substances of the infundibulum can be stained for light- and electron-microscopic examination. In various mammalian species, the size of Gomori-positive elementary granules in the outer layer is markedly different from that in the inn...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218495
更新日期：1975-11-19 00:00:00
