Abstract：
:Transplantation of ligament-tissue-derived stem cells has become a promising approach in the repair of injured ligament. Neovascularization plays an important role in ligament healing and remodeling. Recently, human umbilical-cord-blood-derived CD34+ cells have been reported to contribute to neoangiogenesis. Therefore, we performed a series of experiments to test our hypothesis that the combination of medial collateral ligament stem cells (MCL-SCs) and umbilical-cord-blood-derived CD34+ cells has synergistic effects on tendon healing. MCL-SCs and umbilical-cord-blood-derived CD34+ cells were isolated and cultured. Rat MCL injury was treated by MCL-SCs and/or CD34+ cells. Response to the cell therapy was assessed by gross observation, histological evaluation and biomechanical testing at 2 and 4 weeks after each treatment. Although each cell therapy group induced macroscopic and morphological recovery in healing MCLs, the combined use of MCL-SCs/CD34+ cells led to further improvement in healing quality. Capillary density was significantly higher in the CD34+ cell transplantation groups than in the other groups at week 2. Biomechanical testing demonstrated that the failure load of the healing ligament was greatest in the combination therapy group. The combination of MCL-SCs and CD34+ cells as a cell therapeutic thus enhances healing and restores biomechanical function toward normal after MCL injury. The findings obtained in our study suggest that the combination of MCL-SCs and CD34+ cells transplantation represents a promising strategy for ligament injury.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Jiang D,Yang S,Gao P,Zhang Y,Guo T,Lin H,Geng Hdoi
10.1007/s00441-015-2250-4subject
Has Abstractpub_date
2015-12-01 00:00:00pages
587-95issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-015-2250-4journal_volume
362pub_type
杂志文章abstract：:A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218082
更新日期：1986-01-01 00:00:00
abstract：:Apoptotic and inflammatory processes occur in human arteriosclerotic lesions. We examined the hypothesis whether both processes are possibly associated by studying the colocalization of corresponding markers. In 11 human arteriosclerotic carotid arteries, proapoptotic markers (CPP32 (caspase-3), poly(ADP-ribose) polym...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0024-0
更新日期：2005-12-01 00:00:00
abstract：:The amygdala is a central hub for fear learning assessed by Pavlovian fear conditioning. Indeed, the prevailing hypothesis that learning and memory are mediated by changes in synaptic strength was shown most convincingly at thalamic and cortical afferents to the lateral amygdala. The neurotrophin brain-derived neurotr...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03260-3
更新日期：2020-10-01 00:00:00
abstract：:The hormone-induced pigment dispersion in primary cultures of xanthophores of goldfish (Carassius auratus L.) has been shown to involve the dispersion of not only carotenoid droplets but also of smooth endoplasmic reticulum. The dispersion of these organelles is inhibited by cytochalasin B and is accompanied by thinni...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219555
更新日期：1978-07-10 00:00:00
abstract：:Pulmonary neuroepithelial bodies (NEBs) are extensively innervated organoid groups of neuroendocrine cells that lie in the epithelium of intrapulmonary airways. Our present understanding of the morphology of NEBs is comprehensive, but direct physiological studies have so far been challenging because the extremely diff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1111-y
更新日期：2005-07-01 00:00:00
abstract：:This study provides information on the rates of DNA synthesis, sites of DNA synthesis, and DNA content of the avian salt gland during the osmotic stressing (plasma membrane synthesis) and destressing (plasma membrane turnover) cycle, in an effort to better understand the relationship of cell turnover to the initial ev...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214782
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233895
更新日期：1978-12-29 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0405-7
更新日期：2007-07-01 00:00:00
abstract：:In fresh-water turtles, the bridge connecting the proximal and caudal stumps of transected spinal cords consists of regenerating axons running through a glial cellular matrix. To understand the process leading to the generation of the scaffold bridging the lesion, we analyzed the mitotic activity triggered by spinal i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1173-y
更新日期：2011-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0534-z
更新日期：2008-02-01 00:00:00
abstract：:The ultrastructure of the dermal papulae of a starfish (Asterias rubens) is consistent with a respiratory function. The present study has shown no regions specialized for excretory mechanisms. The papulae consist of an outer ectodermal epithelium of sensory, support and gland cells and a small basiepithelial nerve ple...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229616
更新日期：1978-03-13 00:00:00
abstract：:The protonephridial flame cell of the freshwater planarian Bdellocephala brunnea was studied by electron microscopy. This basketlike cell with luminal cilia and fenestrae is known as a cyrtocyte. Careful examination revealed the actual continuity of the fenestrae over the surface of the cell and the microvillous natur...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237973
更新日期：1980-01-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resportion zone was demonstrated two hours post pulse whereas the grain den...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219726
更新日期：1976-04-28 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051271
更新日期：1999-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226505
更新日期：1988-01-01 00:00:00
abstract：:The formation of secretory granules in chronically hypersecretory melanotrophs in the rat pituitary was studied. Hypersecretion was induced by treatment with the dopamine antagonist haloperidol (1.5 mg/kg daily for 7 days), which releases the normal neural dopaminergic inhibition of secretion from the melanotroph. Mor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319432
更新日期：1994-02-01 00:00:00
abstract：:Purinoceptor subtypes were localised to various tissue types present within the nasal cavity of the rat, using immunohistochemical methods. P2X3 receptor immunoreactivity was localised in the primary olfactory neurones located both in the olfactory epithelium and vomeronasal organs (VNO) and also on subepithelial nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0979-2
更新日期：2005-01-01 00:00:00
abstract：:Tanycytes in the median eminence (ME) of the rat exhibit morphological features suggesting their possible participation in transport phenomena. After adrenalectomy, which modifies the hypothalamo-hypophyseal axis, they undergo morphological changes characterized by an accumulation of lipid droplets, an increased numbe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215708
更新日期：1982-01-01 00:00:00
abstract：:Lysozyme is one of the most prominent antimicrobial peptides and has been identified from many mammalian species. However, this enzyme has not been studied in the order Insectivora, which includes the most primitive placental mammals. Here, we done the lysozyme cDNA from Suncus murinus (referred to as suncus, its labo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-02991-2
更新日期：2019-06-01 00:00:00
abstract：:The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured fro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00323584
更新日期：1993-04-01 00:00:00
abstract：:In recent years, data have accumulated suggesting that the role of neurotrophins and Trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. This study was designed to map the expression of immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000181
更新日期：2000-04-01 00:00:00
abstract：:The isolated perfused heart model was used to examine the structure of rat cardiac capillaries and their permeability to macromolecules of various sizes. Haemoglobin (diameter 6.4 nm) and catalase (10.4 nm) did not cross the endothelium but remained on the luminal side. Cytochrome C (3 nm) and horseradish peroxidase (...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213826
更新日期：1988-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0522-3
更新日期：2008-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214696
更新日期：1982-01-01 00:00:00
abstract：:The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a "subcorneal layer" situated between the corneal lens and crystalline cone. A distal rhabdomere consisting o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233613
更新日期：1980-01-01 00:00:00
abstract：:We develop "autologous bone marrow cell infusion (ABMi) therapy" for the treatment of human decompensated liver cirrhosis and confirm the efficacy and safety of this treatment in multicenter clinical studies. With the goal of further expanding the applications of ABMi, we first cultured human bone marrow cells and the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1727-2
更新日期：2013-12-01 00:00:00
abstract：:Over the past decade, adult stem cells have attracted great attention because of their ability to potentially regenerate desired tissues or entire organs. With the emergence of nanomaterial-based gene therapy, adult stem cells have been considered as a proper tool for the biomedical field. In this study, we utilized o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2235-3
更新日期：2015-12-01 00:00:00
abstract：:The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1353-4
更新日期：2012-04-01 00:00:00
abstract：:Drosophila melanogaster photoreceptors are highly polarized cells and their plasma membrane is organized into distinct domains. Zonula adherens junctions separate a smooth peripheral surface, the equivalent of the basolateral surface in other epithelial cells, from the central surface (approximately equal to apical su...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000195
更新日期：2000-05-01 00:00:00
