Interendothelial junctions of cardiac capillaries in rats: their structure and permeability properties.

Abstract:

:The isolated perfused heart model was used to examine the structure of rat cardiac capillaries and their permeability to macromolecules of various sizes. Haemoglobin (diameter 6.4 nm) and catalase (10.4 nm) did not cross the endothelium but remained on the luminal side. Cytochrome C (3 nm) and horseradish peroxidase (6 nm) both crossed the endothelium to the subendothelial space and filled the caveolae on the abluminal side as well as the entire length of the lateral intercellular spaces. The membranes of the endothelial cells are separated by an intercellular gap of mean width 18.2 nm. At one or more zonular regions within each lateral intercellular space the two membranes approach each other more closely and frequently appear to fuse. However, tilting the specimen shows that, in these regions, there is a gap of mean width 5.4 nm (in lanthanum- and tannic acid-treated tissue, 3.8 nm in ferrocyanide-treated tissue) between the membranes. We conclude that these narrow regions sieve macromolecules on the basis of size although other factors may determine their permeability properties.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Ward BJ,Bauman KF,Firth JA

doi

10.1007/BF00213826

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

57-66

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

252

pub_type

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