Abstract：
:The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation of myenteric neurons and glial cells in paraffin-embedded colonic sections; additional data would be useful for diagnostic purposes. In this morphometric study, we performed immunohistochemistry to count myenteric neurons and glial cells in paraffin sections of human colon. Serial cross sections of formalin-fixed paraffin-embedded full-thickness normal human left colon (n = 10, age-range: 50-72 years) were examined. HuC/D and S100beta antigens were found to be the best markers for the detection of neurons and glial cells, respectively. Significant correlations were noted between the numbers of neurons/glial cells and the respective myenteric ganglion areas. These findings suggest that HuC/D-S100beta-immunostained paraffin cross sections of human colon can be regarded as valuable tools for the quantitative estimation of myenteric neurons and glial cells. Based on the present method, only a limited number of paraffin sections are needed for reliable quantitative assessments of myenteric ganglion cells, thus allowing fast and simple approaches in the settings of the histopathological diagnosis of colonic motility disorders and retrospective evaluations of pathological archival tissue specimens.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ippolito C,Segnani C,De Giorgio R,Blandizzi C,Mattii L,Castagna M,Moscato S,Dolfi A,Bernardini Ndoi
10.1007/s00441-009-0770-5subject
Has Abstractpub_date
2009-05-01 00:00:00pages
191-201issue
2eissn
0302-766Xissn
1432-0878journal_volume
336pub_type
杂志文章abstract：:Electron microscopy of the rat lymph node disclosed reticular cells in close association with bundles of collagenous fibrils 30-45 nm in diameter, and electron lucent "amorphous" substances surrounded by microfibrils 11 nm in diameter, with junctional complexes. The amorphous substances, distinctly revealed by orcein ...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1998-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2018-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218550
更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209040
更新日期：1978-10-17 00:00:00
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journal_title：Cell and tissue research
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更新日期：1988-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0193-5
更新日期：2006-09-01 00:00:00
abstract：:Exposure to nicotine in smoking contributes to most unexplained male infertility but the mechanisms remain to be fully elucidated. Zinc (Zn) is an essential trace element in normal growth, development and reproduction. Zinc oxide nanoparticles (ZnONPs) are well-known antioxidants. Therefore, this work was designed to ...
journal_title：Cell and tissue research
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更新日期：2019-02-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1990-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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