Abstract：
:Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyLu and the AT-2 cell line for 24 h with the Na+ channel blocker tetrodotoxin (TTX) at 6 microM altered the morphology only of the MAT-LyLu cell line. TTX produced significant decreases in: (a) cell process length and (b) field diameter, and increases in (c) cell body diameter and (d) process thickness. Importantly, 6 microM TTX had no significant effects on proliferation rates or cellular toxicity. The results suggest that Na+ channel activity plays a significant role in determining the morphological development of MAT-LyLu cells in such a way as to enhance their metastatic potential.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Fraser SP,Ding Y,Liu A,Foster CS,Djamgoz MBdoi
10.1007/s004410051256keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
505-12issue
3eissn
0302-766Xissn
1432-0878journal_volume
295pub_type
杂志文章abstract：:Large conductance calcium-activated potassium channels (BK(ca)) are crucial for the regulation of cerebral vascular basal tone and might be involved in cerebral vasodilation relevant to migraine and stroke. We studied the differential gene expression of mRNA transcript levels and protein expression of the BK(Ca) chann...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0701-x
更新日期：2008-12-01 00:00:00
abstract：:Neurotrophins NGF, BDNF, NT-3, and NT-4/5 are members of the neurotrophin family of proteins, which support the survival and induce differentiation of vertebrate neurons. We have studied the effects of neurotrophins on growth cones of embryonic sensory neurons. BDNF and NT-4/5 cause growth cone collapse and transient ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050933
更新日期：1997-11-01 00:00:00
abstract：:The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219054
更新日期：1977-03-09 00:00:00
abstract：:Clusterin (CLU) is an extracellular chaperone protein that is implicated in diverse physiological and pathophysiological cellular processes. CLU expression is upregulated in response to cellular stress and under certain conditions, such as neurodegenerative disease and cancer. CLU primarily functions as a chaperone th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2650-8
更新日期：2017-10-01 00:00:00
abstract：:The innervation pattern of distal muscle fibers of the opener muscle of walking legs of crayfish (Astacus leptodactylus) was investigated using methylene-blue staining, cobalt infiltration, and electron microscopy. A quantitative analysis of the entire innervation of single muscle fibers was attempted. It was found th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213750
更新日期：1982-01-01 00:00:00
abstract：:The influence of adrenalectomy and administration of hypertonic saline on the amount of vasopressin, oxytocin, and neurophysin contained in the median eminence and the neural lobe of rats was studied by means of the following methods: (i) morphometric and microphotometric analyses of aldehyde fuchsin-stained histologi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00206271
更新日期：1983-01-01 00:00:00
abstract：:Wnts are secreted glycoproteins that play multiple roles in early development, including the differentiation of precursor cells. During this period, gradients of Wnts and other morphogens are formed and regulate the differentiation and migration of neural progenitor cells. Afterwards, Wnt signalling cascades participa...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1996-4
更新日期：2015-01-01 00:00:00
abstract：:The innervation of the anococcygeus muscle of the rat was investigated with regard to the histochemical features of nerve fibers within the muscle and to the location of the postganglionic autonomic neurons which are the source of these fibers. Acetylcholinesterase-positive fibers and catecholaminergic fibers are abun...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00571438
更新日期：1990-01-01 00:00:00
abstract：:The present study continues a previous investigation on the median eminence (EM) (Krisch et al., 1978). In rats with high levels of neurohormones (LHRH, vasopressin) a limited immunohistochemical labeling of perivascular tanycyte processes can be observed surrounding capillaries in the marginal region of the organum v...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233891
更新日期：1978-12-29 00:00:00
abstract：:Injection of wheat-germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the superior cervical ganglion (SCG) of the rat results in accumulation of WGA-HRP in sympathetic postganglionic neurons in the contralateral SCG. The sympathetic pathways involved and the mechanism underlying the labeling were investig...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327745
更新日期：1990-10-01 00:00:00
abstract：:Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture repicas from ra...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237626
更新日期：1980-01-01 00:00:00
abstract：:Cytochemical methods for the demonstration of p-nitrophenylphosphatase (p-NPPase) at the electron-microscopic level were applied to avian osteoclasts to elucidate some of the functional differences among ruffled border, other membrane systems, and cellular organelles. The localization of p-NPPase activity occurred in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218381
更新日期：1986-01-01 00:00:00
abstract：:Dorsal root ganglia of chick embryos were cultured for one to four weeks on Maximow-slides. Puromycin was added to cultures for a pulse of 30' in a dose of 100 mug/ml medium. Particular interest was given to the ultrastructural features of the glia-neuron relations. Puromycin caused a shrinkage of the glial processes ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225762
更新日期：1975-07-23 00:00:00
abstract：:In the intestine of the filtering zooids of B. schlosseri three segments can be distinguished. In the intermediate segment the epithelium, which is encrusted by the ampullae of the pyloric gland, shows marked aspects of alteration. In the proximal and distal segments, ciliated mucous, vacuolated and endocrine-like cel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219771
更新日期：1977-08-26 00:00:00
abstract：:The apposition compound eyes of gonodactyloid stomatopods are divided into a ventral and a dorsal hemisphere by six equatorial rows of enlarged ommatidia, the mid-band (MB). Whereas the hemispheres are specialized for spatial vision, the MB consists of four dorsal rows of ommatidia specialized for colour vision and tw...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1118-4
更新日期：2005-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-2011-9
更新日期：2015-01-01 00:00:00
abstract：:We have previously shown that immunoneutralization of transforming growth factor-beta (TGF-beta) in the chick embryo significantly reduces programmed cell death (PCD) in peripheral neurons, spinal cord, and retina. In order to validate these results we have begun to analyze PCD in mice with targeted ablations of the T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0761-x
更新日期：2003-07-01 00:00:00
abstract：:The origin and renewal of the argentaffin cells in the pyloric glands of hamsters were studied by flash, cumulative and pulse labelling autoradiography with 3H-thymidine. The argentaffin cells were identified by the Diazo Method using Fast Red B Salt. By flash labelling autoradiography, it was shown that the argentaff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222771
更新日期：1977-06-20 00:00:00
abstract：:The immunoreactive CRF-neurons of the rat hypothalamus have been examined immunohistochemically employing anti-rat CRF serum. These neurons are confined to the paraventricular nucleus, dorsomedial-lateral hypothalamic area, and suprachiasmatic nucleus, and are, respectively, also immunoreactive to anti-Met-enk, -alpha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216347
更新日期：1985-01-01 00:00:00
abstract：:The ontogeny of two gonadotropin-releasing-hormone (GnRH) systems, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), was investigated in zebrafish (Danio rerio). In situ hybridization (ISH) first detected sGnRH mRNA-expressing cells at 1 day post-fertilization (pf) anterior to the developing olfactory organs. Subseq...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0279-0
更新日期：2007-02-01 00:00:00
abstract：:Cortisone-treated Buffalo rats have been parabiosed with untreated controls of the same age. The optical and electron microscopy, including histochemistry, of costal cartilage of these rats has been compared with that in single cortisone treated rats, single controls, and control parabiosed with control rats, at 14 an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223122
更新日期：1978-05-18 00:00:00
abstract：:Dendritic cells are migratory cells. Before they extravasate from the circulation into the skin across capillary blood vessel walls, they have to interact with endothelial cells. Using a fluorimetric adhesion assay, we have recently shown that CD34(+)-derived dendritic cell precursors are able to bind to resting and s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0780-7
更新日期：2004-01-01 00:00:00
abstract：:By means of single and double immuno-enzyme cytochemical staining techniques, it was shown that the external region of the amphibian median eminence contains separate vasotocinergic and mesotocinergic nerve fibres. Moreover, it was demonstrated that the vasotocinergic fibres also contain neurophysin. In animals in whi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221117
更新日期：1977-02-02 00:00:00
abstract：:The distribution of P2X(2) purine receptor subunit immunoreactivity has been investigated in the mouse gastrointestinal tract. Immunoreactivity occurred in intraganglionic laminar endings (IGLEs) associated with myenteric ganglia throughout the gastrointestinal tract. In the esophagus, IGLEs supplied every myenteric g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0715-3
更新日期：2003-05-01 00:00:00
abstract：:We have analyzed the distribution of neural crest-derived precursors and the expression of catecholaminergic and neuronal markers in developing adrenal tissue of chick embryos. Undifferentiated neural crest cells are found in presumptive adrenal regions from embryonic day 3 (E3) onward. An increasing proportion of cel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0996-1
更新日期：2005-01-01 00:00:00
abstract：:By use of the indirect immunofluorescence technique a small group of large somatostatin-positive neurons is described in the subependymal area of the anterior paraventricular thalamus of the male rat. Retrograde-tracing experiments suggest that they project to areas outside the blood-brain barrier. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215212
更新日期：1986-01-01 00:00:00
abstract：:Early diplotene oocytes from Necturus maculosus ranging from approximately 0.2 to 0.5 mm in diameter were examined by electron microscopy. In the smallest oocytes of this range, the cytoplasm is largely devoid of membranes, but contains primarily ribosomes and mitochondria. In slightly larger oocytes, smooth-surfaced ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218087
更新日期：1986-01-01 00:00:00
abstract：:In three species of teleosts - carp Cyprinus carpio; grass carp Ctenopharyngodon idella; and crucian carp Carassius auratus - the caudal neurosecretory system displays small, medium-sized and large neurons. Urotensin I (UI)-immunoreactive and UI-nonreactive neurons were found in all three groups; in general, the numbe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212550
更新日期：1986-01-01 00:00:00
abstract：:Transgenic rats for the murine Ren-2 gene display high blood pressure, low circulating levels of angiotensin II, and high renin content in the adrenal glands. Moreover, transgenic rats possess an increased aldosterone secretion (maximal from 6 to 18 weeks of age), paralleling the development of hypertension. To invest...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305774
更新日期：1994-10-01 00:00:00
abstract：:Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222069
更新日期：1975-01-01 00:00:00
