Abstract：
:Cytochemical methods for the demonstration of p-nitrophenylphosphatase (p-NPPase) at the electron-microscopic level were applied to avian osteoclasts to elucidate some of the functional differences among ruffled border, other membrane systems, and cellular organelles. The localization of p-NPPase activity occurred in mitochondria, in lysosomes, and on the cytoplasmic side of the ruffled-border membrane. Enzymatic activity in the ruffled-border membrane was sensitive to ouabain and was partially dependent on potassium. Thus, Na+,K+-ATPase, the proposed sodium pump, appears to be present in the osteoclast ruffled border. The activity in ruffled border occurred only when osteoclasts were attached to bone. Following calcitonin treatment many osteoclasts were detached from the bone surface and lacked reaction product along the ruffled-border membrane. The lysosomal p-NPPase activity was not sensitive to ouabain and was distinct from that of other lysosomal phosphatases. The p-NPPase activity in mitochondria was not inhibited by ouabain but was sensitive to duramycin. The mitochondrial p-NPPase may, therefore, represent the mitochondrial proton pump.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Akisaka T,Gaỳ CVdoi
10.1007/BF00218381subject
Has Abstractpub_date
1986-01-01 00:00:00pages
57-62issue
1eissn
0302-766Xissn
1432-0878journal_volume
244pub_type
杂志文章abstract：:The cerebral ganglia of the freshwater snail Lymnaea stagnalis contain two clusters of neurosecretory Caudo-Dorsal Cells (CDC). These cells produce a neurohormone which stimulates ovulation. Ganglion transplantation and quantitative electron microscopy show that neuronal isolation of the cerebral ganglia complex (CCC)...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219720
更新日期：1976-04-28 00:00:00
abstract：:The mole has a single pair of accessory sex organs with features of both the prostate and the seminal vesicle, for which the term prostate gland is not appropriate. Seasonal changes occuring in this gland were related to four periods: a) the quiescence period, b) the maturation period, c) the active period and d) the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218718
更新日期：1976-12-10 00:00:00
abstract：:Changes in the ultrastructure of the toad epidermis during the moulting cycle are described on the basis of 17 skin preparations fixed in consecutive phases of the cycle. Our previous light microscopical findings that morphological changes are mainly restricted to a short period prior to and after shedding are confirm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221703
更新日期：1975-06-24 00:00:00
abstract：:Thin sections after bile duct ligation showed that the depth of tight junctions appeared to increase and that the distance between individual punctate contacts appeared to become irregular and wider than in controls. The freeze fracture replicas clearly demonstrated these changes in the tight junction morphology. Chan...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236724
更新日期：1978-12-28 00:00:00
abstract：:SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1618-6
更新日期：2013-06-01 00:00:00
abstract：:Palatal shelf elevation is a crucial process in palate development, with the contribution of various factors. Disturbances in any factor during this process result in cleft palate. Prior to palatal shelf elevation starting from embryonic day 12.5, the Rac1 expression level in the bend region of the mid-palatal shelf p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2169-9
更新日期：2015-09-01 00:00:00
abstract：:CYCLE (CYC) and CLOCK (CLK) are transcriptional activators of the circadian clock genes, period (per) and timeless (tim), binding at E-boxes of their upstream regulatory region in Drosophila. CYC-like and CLK-like immunohistochemical reactivities (CYC-ir and CLK-ir) were investigated in the ground cricket, Allonemobiu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0534-z
更新日期：2008-02-01 00:00:00
abstract：:In previous studies the differentiation of the epithelium in the human hard palate has been described stereologically using parameters expressed per unit tissue volume. Since single epithelial cells represent the true biological units of this tissue, it became necessary to estimate the absolute size of such cells in o...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216577
更新日期：1981-01-01 00:00:00
abstract：:Recently, an antibody against the choline transporter (CHT), an essential molecule involved in ACh uptake, was used to label cholinergic nerves in the central nervous system; however, the enteric nervous system (ENS) was not examined. The present study localised CHT immunoreactivity (CHT-IR) within the rat ileum ENS a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0332-z
更新日期：2007-03-01 00:00:00
abstract：:The so-called 'basement membrane' of arthropod compound eyes is known to be of heterogeneous origin (Odselius and Eloffson 1981). A major contribution in Diptera with open rhabdoms is provided by a pigmented component which lies at the basal end of the extracellular space of each ommatidium and fills it, the glial plu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222184
更新日期：1983-01-01 00:00:00
abstract：:Intercellular communication between smooth muscle cells is crucial for contractile behaviour in normal and pathologically altered urinary bladder. Since the study of coupling is difficult in situ, we established cell cultures of bladder smooth muscle cells to analyse coupling mechanisms. Microinjection of Lucifer yell...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0559-2
更新日期：2002-08-01 00:00:00
abstract：:The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicle...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100351
更新日期：2001-04-01 00:00:00
abstract：:Recent evidence indicates that many molecules involved in generating and patterning the limbs also play a role during craniofacial morphogenesis. On the surface, this is an unexpected finding given that these regions of the body have separate evolutionary origins, are composed of different embryonic tissues, and are q...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051271
更新日期：1999-04-01 00:00:00
abstract：:Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217578
更新日期：1985-01-01 00:00:00
abstract：:Our previous findings have demonstrated that the rat autosomal-recessive mutation, whitish chalk-like teeth (wct), induces enamel defects resembling those of human amelogenesis imperfecta (AI) in continuously growing incisor teeth. The present study clarifies the effect of the wct mutation on the morphogenesis and cal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0452-0
更新日期：2007-10-01 00:00:00
abstract：:Rat thymic phagocytic cells were characterized in vitro using various light- and electron-microscopical techniques. Thymic cell suspensions were mechanically prepared and enriched for non-lymphoid cells, which were predominantly phagocytic and of three types. Type I showed acid phosphatase (APh) activity in small gran...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222183
更新日期：1983-01-01 00:00:00
abstract：:Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultrastructural similar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221380
更新日期：1976-10-06 00:00:00
abstract：:This study aims to illustrate potential transport mechanisms behind the divergent approaches to nitrogen excretion seen in the ureotelic toadfish (Opsanus beta) and the ammoniotelic plainfin midshipman (Porichthys notatus). Specifically, we wish to confirm the expression of a urea transporter (UT), which is found in t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1591-0
更新日期：2013-06-01 00:00:00
abstract：:In cattle, the oviduct plays a major role in the reproductive process; however, molecular control of oviduct receptivity to the embryo is poorly understood. A model for receptivity based on size of the pre-ovulatory follicle (POF) was used to compare oviductal morphology, cellular proliferation, and candidate transcri...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2666-0
更新日期：2017-11-01 00:00:00
abstract：:Alcohol and aldehyde dehydrogenases (ADHs and ALDHs) may be of interest in the pathology of Parkinson's disease (PD) because of their role in protection against toxins and in retinoid metabolism, which is required for growth and development of the mesencephalic dopamine system. In the present study, the spatial and te...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0038-7
更新日期：2005-11-01 00:00:00
abstract：:Angiogenesis, the growth of new blood vessels, is required for a variety of normal proliferative processes. Furthermore, angiogenesis is well established as also playing an important role in neoplastic growth and metastasis. Numerous regulators of angiogenesis have been identified and characterized over the last few d...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0675-8
更新日期：2009-01-01 00:00:00
abstract：:The production of nerve growth factor (NGF) in peripheral organs may play a role in the pathophysiology of hypertension and in obstructive disorders of the bladder outlet. We have been examining the cellular processes of NGF delivery and secretion in smooth muscle. NGF secretion from vascular smooth muscle cells (VSMC...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050861
更新日期：1997-07-01 00:00:00
abstract：:The genes encoding transmembrane glycoproteins of the cadherin family, i.e., the Ca(2+)-dependent cell-cell adhesion molecules, are typically expressed in cell-type- or cell-lineage-specific patterns. One of them, vascular endothelial (VE)-cadherin, is widely considered to be specific for vascular endothelia in which ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0718-1
更新日期：2009-01-01 00:00:00
abstract：:Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234326
更新日期：1979-11-01 00:00:00
abstract：:Cortisone-treated Buffalo rats have been parabiosed with untreated controls of the same age. The optical and electron microscopy, including histochemistry, of costal cartilage of these rats has been compared with that in single cortisone treated rats, single controls, and control parabiosed with control rats, at 14 an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223122
更新日期：1978-05-18 00:00:00
abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP3R1) is predominantly expressed in cereb...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307790
更新日期：1995-05-01 00:00:00
abstract：:In the present study, we characterized intra-epithelial leukocytes in the digestive tract of chickens during postnatal development. Their phenotype was characterized by monoclonal antibodies in cryostat sections and the numbers of the different cell-types were counted in the epithelium of the esophagus, proventriculus...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318748
更新日期：1993-11-01 00:00:00
abstract：:This review focuses on the signaling system involving the Angiopoietin1/Tie2 receptor, which appears to be involved in the secondary stages of blood vessel formation. Although this system is crucial for blood and lymphatic vessel formation, identifying its precise role in embryonic and adult vascular biology has been ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-003-0749-6
更新日期：2003-10-01 00:00:00
abstract：:Intra-arterial injections of synthetic double-stranded RNA (polyinosinic:polycytidylic acid, PIPC) at a dose of 500 microg/kg evoked pronounced fever in guinea-pigs. PIPC-induced fever could be antagonized by treatment with the non-selective cyclooxygenase (COX) inhibitor diclofenac and was, in part, attenuated by the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0386-6
更新日期：2007-06-01 00:00:00
abstract：:Type-A spermatogonia can be found in different locations throughout the germinal epithelium of human seminiferous tubules. Generally they represent the population of germ cells adjacent to the basal lamina, but under special conditions they may be located in the adluminal compartment. This aberrant location is found n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216510
更新日期：1984-01-01 00:00:00
