Abstract:
:Neurotrophins NGF, BDNF, NT-3, and NT-4/5 are members of the neurotrophin family of proteins, which support the survival and induce differentiation of vertebrate neurons. We have studied the effects of neurotrophins on growth cones of embryonic sensory neurons. BDNF and NT-4/5 cause growth cone collapse and transient neurite growth inhibition in NGF-dependent or NT-3-dependent rat dorsal root ganglion (DRG) neurons but not in BDNF-dependent or NT-4/5-dependent neurons, whereas NGF and NT-3 do not produce growth-cone collapse in these neurons. All neurotrophins show a chemoattractive effect on growth cones of embryonic DRG neurons: NGF and NT-3 are chemoattractants for all DRG neurons, except NT-3-dependent and NT-4/5-dependent neurons; BDNF and NT-4/5 are chemoattractants only for BDNF-dependent DRG neurons. BDNF-induced and NT-4/5-induced growth cone collapse is quantitatively characterized as a 50% decrease in F-actin content, total protein content, and area of growth cones of NGF-dependent or NT-3-dependent neurons, and a reorganization of microfilaments. BDNF induces a rapid transient 3-fold to 4-fold increase of F-actin concentration at the central part of growth cones of NGF-dependent neurons. Our results suggest that different neurotrophins have chemoattractive or inhibitory effects on the same growth cone, and that they may act as specific growth cone guidance cues.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Paves H,Saarma Mdoi
10.1007/s004410050933subject
Has Abstractpub_date
1997-11-01 00:00:00pages
285-97issue
2eissn
0302-766Xissn
1432-0878journal_volume
290pub_type
杂志文章,评审abstract::Two-month-old female Swiss mice that had come into estrus were injected intravenously with L-3H-fucose and killed at 5, 15, 40 min, and 4 h after injection. Pieces of the isthmus and of the ampulla of the uterine tubes were processed for light- and electron-microscopic radioautography. Incorporation of 3H-fucose was m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220036
更新日期:1988-10-01 00:00:00
abstract::A study of the organisation of the locust dorsal ocellus shows that the structure is designed to provide the maximum possible effective aperture. The condenser-like cuticular lens and the dispersal of the rhabdome over a large proportion of the circumferential area of the retinula cells increases the light gathering p...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234742
更新日期:1979-02-28 00:00:00
abstract::Intra-arterial injections of synthetic double-stranded RNA (polyinosinic:polycytidylic acid, PIPC) at a dose of 500 microg/kg evoked pronounced fever in guinea-pigs. PIPC-induced fever could be antagonized by treatment with the non-selective cyclooxygenase (COX) inhibitor diclofenac and was, in part, attenuated by the...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0386-6
更新日期:2007-06-01 00:00:00
abstract::Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparatio...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217847
更新日期:1984-01-01 00:00:00
abstract::We chose synaptic terminals of rat rod bipolar cells as a model system to study activity-related changes in the overall morphology and the fine structure of synaptic sites. Using confocal laser scanning microscopy in conjunction with three-dimensional reconstruction and electron microscopy, we examined the effect of l...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051174
更新日期:1998-11-01 00:00:00
abstract::In vitro incubation of autonomic nerves with vinblastine sulphate (1 times 10(-4) M) caused a disappearance of microtubules within 15 min; during the following 15 min paracrystalline arrays appeared within the axons. An increase in the abundance of microfilaments was also observed, but these did not appear to arise fr...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220136
更新日期:1976-02-25 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::Glutamate is the major excitatory neurotransmitter of the mammalian retina and glutamate uptake is essential for normal transmission at glutamatergic synapses. The reverse transcriptase-polymerase chain reaction (RT-PCR) has revealed the presence of three different high-affinity glutamate transporters in the rat retin...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050976
更新日期:1998-01-01 00:00:00
abstract::More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217555
更新日期:1985-01-01 00:00:00
abstract::It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218485
更新日期:1982-01-01 00:00:00
abstract::The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219054
更新日期:1977-03-09 00:00:00
abstract::The distribution pattern of adrenergic fibres innervating the ocular choroid membrane of the chicken was studied by means of fluorescence and electron microscopy. In addition, the origin of these fibres was investigated after superior cervical ganglionectomy. Adrenergic axons reach the choroid, partly forming the peri...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213222
更新日期:1982-01-01 00:00:00
abstract::Whole pigs lenses were fixed, critical-point dried and fractured, and the internal surfaces examined with a scanning electron microscope. At various locations from the equator to the center of the lens four types of fibers can be distinguished. The superficial fibers have small interdigitations. Cortical lens fibers, ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217281
更新日期:1982-01-01 00:00:00
abstract::Vasodilator-stimulated phosphoprotein (VASP) and mammalian-enabled protein (MENA) share similar cellular localisation and functions (signal transduction pathways, regulation of actin cytoskeleton dynamics). Functional substitution and compensation among Ena/VASP proteins have been proposed as the reason for the absenc...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0964-9
更新日期:2004-12-01 00:00:00
abstract::The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218204
更新日期:1987-05-01 00:00:00
abstract::The wall of the seminiferous tubule in rodents consists of an inner layer of myoid cells covered by an outer layer of endothelial cells. Myoid cells are a type of smooth muscle cell containing α-actin filaments arranged in two independent layers that contract when stimulated by endothelin-1. The irregular surface reli...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-016-2386-x
更新日期:2016-08-01 00:00:00
abstract::The monoamines octopamine and tyramine, which are the invertebrate counterparts of epinephrine and norepinephrine, transmit their action through sets of G protein-coupled receptors. Four different octopamine receptors (Oamb, Octß1R, Octß2R, Octß3R) and 3 different tyramine receptors (TyrR, TyrRII, TyrRIII) are present...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2137-4
更新日期:2015-09-01 00:00:00
abstract::Subsurface cisterns (SSC's) and less frequent lamellar bodies (LB's) were identified in the granule cells of the guinea-pig fascia dentata. Both structures, composed of flattened or collapsed agranular cisterns, are continuous with the regular rough endoplasmic reticulum and occasionally connected with each other form...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF01258502
更新日期:1982-01-01 00:00:00
abstract::The spermatogonial stem cell line in prepubertal and adult bovine testis was studied by electron microscopy and protein gene product 9.5 immunohistochemistry. Three successive spermatogonia precursor cell configurations were observed. Small basal stem cells were found to possess a spherical shape and nuclei with two t...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00583394
更新日期:1995-08-01 00:00:00
abstract::The effects of the administration of thyroxine (T4) on the postnatal cytodifferentiation of granular convoluted tubule (GCT) cells of the submandibular gland (SMG) of Lewiss-Webster mice were studied by light and electron microscopy. From birth, mice of both sexes were injected daily with T4 (sc 0.4 micrograms/g BW) a...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218202
更新日期:1987-05-01 00:00:00
abstract::Regions within frog semitendinosus muscle that are rich in tonic muscle cells were identified histochemically by myosin adenosine triphosphatase- and succinic dehydrogenase-staining procedures. Bundles of cells still attached to tendinous insertions were removed from those sites, prepared for electron microscopy and s...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213937
更新日期:1986-01-01 00:00:00
abstract::There is a current need for a therapy that can alleviate the social and economic burden that presents itself with debilitating and recurring musculoskeletal soft tissue injuries and disorders. Currently, several therapies are emerging and undergoing trials in animal models; these focus on the manipulation and administ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03016-8
更新日期:2019-08-01 00:00:00
abstract::Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independen...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00306123
更新日期:1994-05-01 00:00:00
abstract::Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-014-1839-3
更新日期:2014-07-01 00:00:00
abstract::The pars tuberalis of the hypophysis of the Djungarian hamster, Phodopus sungorus, was investigated with regard to secretory activity by applying the tannic acid-Ringer perfusion technique. Two groups were maintained under long photoperiods (16 h light: 8 h dark) or short photoperiods (8 h light: 16 h dark), respectiv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00312830
更新日期:1993-08-01 00:00:00
abstract::The neurosecretory cells of the ventral ganglia in the adult dragonfly, Orthetrum chrysis, are classified into A, B, C1 and C2 cells. The neurosecretory material in the ventral ganglia is composed of PAS-positive material with 1-, 2-glycol groups and some proteins. The proteins rich in cystine or cysteine occur predom...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221087
更新日期:1977-02-09 00:00:00
abstract::The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221498
更新日期:1982-01-01 00:00:00
abstract::Filamentous intranuclear rodlets were found in a majority of cells in a bronchiolar neuroepithelial body of a rabbit. These rodlets appear to be similar to structures that have been described primarily in neural tissues. They are composed of large bundles of microfibrils made up of smaller subbundles. No close associa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210521
更新日期:1981-01-01 00:00:00
abstract::Pulmonary neuroepithelial bodies (NEBs) are extensively innervated organoid groups of neuroendocrine cells that lie in the epithelium of intrapulmonary airways. Our present understanding of the morphology of NEBs is comprehensive, but direct physiological studies have so far been challenging because the extremely diff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1111-y
更新日期:2005-07-01 00:00:00
abstract::A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218082
更新日期:1986-01-01 00:00:00