Dictyosome-like structures from guinea-pig testes lack galactosyltransferase, a Golgi apparatus marker.

Abstract:

:More than twenty different enzyme activities of fractions containing dictyosome-like structures (DLS) as a dominant cell component were monitored. Plasma membrane vesicles were a major contaminant of the DLS fractions, which, presumably as a consequence, were enriched somewhat in plasma membrane markers. The lysosomal enzymes arylsulfatase and latent acid phosphatase were present in the DLS fractions as were the Golgi apparatus activities thiamine pyrophosphatase and nucleoside diphosphatase. The presence of the latter two enzymes in DLS, plus NADH-ferricyanide reductase, has been verified from cytochemistry. On the other hand, the Golgi apparatus marker, galactosyltransferase, was not enriched in DLS fractions and appeared to be absent. This latter finding, verified from cytochemistry with isolated DLS fractions and, in situ, from [3H]galactose incorporation by testis tubules with analysis by autoradiography, provides the first clear biochemical characteristic that serves unequivocally to distinguish DLS from conventional Golgi apparatus.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Morré DJ,Matyas GR,Mollenhauer HH

doi

10.1007/BF00217555

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

35-40

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

240

pub_type

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