Spatial organization and fine structure of the cortical filament layer in normal locomoting Amoeba proteus.

Abstract:

:The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-actin, followed by careful dehydration and freeze-substitution. The filament layer is continuous along the entire surface; it exhibits a varying thickness depending on the cell polarity, measuring several nm in advancing regions and 0.5-1 micron in retracting ones. Two different types of filaments are responsible for the construction of the layer: randomly distributed thin (actin) filaments forming an unordered meshwork beneath the plasma membrane, and thick (myosin) filaments mostly restricted to the uroid region in close association with F-actin. The cortical filament layer generates the motive force for amoeboid movement by contraction at posterior cell regions and induces a pressure flow that continues between the uroid with a high hydrostatic pressure and advancing pseudopodia with low one. The local destabilization of the cell surface as a precondition for the formation of pseudopodia is enabled by the detachment of the cortical filament layer from the plasma membrane. This results in morphological changes by the active separation of peripheral hyaloplasmic and central granuloplasmic regions.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Stockem W,Hoffmann HU,Gawlitta W

doi

10.1007/BF00215699

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

505-19

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

221

pub_type

杂志文章
  • Global daily dynamics of the pineal transcriptome.

    abstract::Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-010-1094-1

    authors: Bustos DM,Bailey MJ,Sugden D,Carter DA,Rath MF,Møller M,Coon SL,Weller JL,Klein DC

    更新日期:2011-04-01 00:00:00

  • The ultrastructure of an olfactory sensillum on the maxillary palps of locusta migratoria (L.).

    abstract::The olfactory sensilla on the maxillary palp tip of Locusta migratoria (L.) resemble the surrounding contact chemoreceptors in general morphology. The perforated peg has a thicker wall than is commonly found in olfactory sensilla. The form and position of the sensilla are considered in relation of the olfactory functi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219899

    authors: Blaney WM

    更新日期:1977-11-07 00:00:00

  • An in vivo model of prostate carcinoma growth and invasion in bone.

    abstract::Prostatic carcinoma affects 1 in 11 men and targets bone with sclerotic metastases. The study of prostate carcinoma growth in bone has been hampered by the lack of suitable animal models. We have developed an in vivo model of prostate carcinoma growth in bone by inoculating three human prostate carcinoma cell lines (P...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-001-0503-x

    authors: Fisher JL,Schmitt JF,Howard ML,Mackie PS,Choong PF,Risbridger GP

    更新日期:2002-03-01 00:00:00

  • Nerve growth factor receptor signaling in proliferation of normal adult rat chromaffin cells.

    abstract::Adult rat chromaffin cells may proliferate or extend neurites when stimulated by nerve growth factor (NGF) but their response is predominantly proliferative, making them a unique model for studying how mitogenic specificity is achieved. We examined contributions of the NGF receptors trk and p75 and of the major NGF si...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410051209

    authors: Powers JF,Shahsavari M,Tsokas P,Tischler AS

    更新日期:1999-01-01 00:00:00

  • Immunohistochemical demonstration of renin in the juxtaglomerular apparatus of three Bufo species.

    abstract::The cellular localization of renin was examined in the kidneys of some amphibians of the genus Bufo by immunoperoxidase and immunofluorescence techniques with an antiserum to renin isolated from the submandibular gland of the mouse. Immunoreactivity could be demonstrated in the media cells of the afferent arterioles (...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219248

    authors: Lamers AP,Verhofstad AA,Stadhouders AM,Michelakis AM

    更新日期:1985-01-01 00:00:00

  • Light and electron microscopic study on the endocrine cells of the pancreas in a marine teleost, Fugu rubripes rubripes.

    abstract::The pancreatic endocrine tissue of Fugu rubripes rubripes consists of numerous round principal islets (Brockmann bodies) of various sizes scattered around the gall-bladder. The endocrine cells are divided into A-, B-, D-, and Ff-cells. Each cell type was identified by comparing thick and thin sections in both light an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222157

    authors: Kobayashi K,Shibasaki S,Takahashi Y

    更新日期:1976-10-29 00:00:00

  • Embryonic chicken gizzard: smooth muscle and non-muscle myosin isoforms.

    abstract::Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independen...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00306123

    authors: Paul ER,Christian AL,Franke R,Gröschel-Stewart U

    更新日期:1994-05-01 00:00:00

  • An ultrastructural morphometric study of the papillary muscle of the right ventricle of the cat.

    abstract::The papillary muscle of the cat heart's right ventricle has not been studied previously with quantitative ultrastructural techniques despite its wide use for functional studies. This tissue was perfusion-fixed, processed for electron microscopy, and morphometric techniques were used to assess the ultrastructural chara...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00216200

    authors: Marino TA,Houser SR,Martin FG,Freeman AR

    更新日期:1983-01-01 00:00:00

  • The retina of the phalangid, Opilio ravennae, with particular reference to arhabdomeric cells.

    abstract::The retina of the phalangid, Opilio ravennae, consists of retinula cells with distal rhabdomeres, arhabdomeric cells, and sheath cells. The receptive segment of retinula cells shows a clear separation into a proximal rhabdom, organized into distinct rhabdom units formed by three or four retinula cells, and a distal rh...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233658

    authors: Schliwa M

    更新日期:1979-01-01 00:00:00

  • NBCe1 (SLC4A4) a potential pH regulator in enamel organ cells during enamel development in the mouse.

    abstract::During the formation of dental enamel, maturation-stage ameloblasts express ion-transporting transmembrane proteins. The SLC4 family of ion-transporters regulates intra- and extracellular pH in eukaryotic cells by cotransporting HCO3 (-) with Na(+). Mutation in SLC4A4 (coding for the sodium-bicarbonate cotransporter N...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-014-1935-4

    authors: Jalali R,Guo J,Zandieh-Doulabi B,Bervoets TJ,Paine ML,Boron WF,Parker MD,Bijvelds MJ,Medina JF,DenBesten PK,Bronckers AL

    更新日期:2014-11-01 00:00:00

  • Respiratory epithelial cells in innate immunity to influenza virus infection.

    abstract::Infection by influenza virus leads to respiratory failure characterized by acute lung injury associated with alveolar edema, necrotizing bronchiolitis, and excessive bleeding. Severe reactions to infection that lead to hospitalizations and/or death are frequently attributed to an exuberant host response, with excessiv...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审

    doi:10.1007/s00441-010-1043-z

    authors: Sanders CJ,Doherty PC,Thomas PG

    更新日期:2011-01-01 00:00:00

  • Calcite in the statoconia of amphibians: a detailed analysis in the frog Rana esculenta.

    abstract::The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00222230

    authors: Marmo F,Balsamo G,Franco E

    更新日期:1983-01-01 00:00:00

  • Fine structure of the pineal organ in the troglobytic fish, Typhlichthyes subterraneous (Pisces: Amblyopsidae).

    abstract::The pineal organ of the blind, cave-dwelling fish, Typhlichythyes subterraneous, was examined with both light and electron microscopes. Like the eyes, the pineal in this troglobytic species was found to be regressed. Two cell types, photoreceptor and supportive cells, were described in the pineal epithelium. Although ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233895

    authors: McNulty JA

    更新日期:1978-12-29 00:00:00

  • Chiton integument: ultrastructure of the sensory hairs of Mopalia muscosa (Mollusca: Polyplacophora).

    abstract::The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221498

    authors: Leise EM,Cloney RA

    更新日期:1982-01-01 00:00:00

  • A scanning electron microscopic study of the rat liver sinusoid: endothelial and Kupffer cells.

    abstract::The surface ultrastructure of Kupffer cells in the rat liver has been studied by scanning electron microscopy (SEM). The results demonstrate that Kupffer cells are both significantly different and clearly distinct from endothelial cells. Kupffer cells have neither pores (and/or "sieve plates") nor fenestrations, all o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00223015

    authors: Motta P

    更新日期:1975-12-10 00:00:00

  • Light- and electron-microscopic studies on the secretory cytology of the adenohypophysis of the Japanese quail, Coturnix coturnix japonica.

    abstract::The effects of thyroidectomy, adrenalectomy, and castration on the pars distalis of male Japanese quail, and of injection of LH-RH on sexually inactive females, were investigated by light and electron microscopy. Correlation between light and electron microscopy was attained by use of alternate thin and thick sections...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00219152

    authors: Mikami S,Kurosu T,Farner DS

    更新日期:1975-06-09 00:00:00

  • Tooth development in vitro in two teleost fish, the cichlid Hemichromis bimaculatus and the cyprinid Danio rerio.

    abstract::A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-004-1036-x

    authors: Van der Heyden C,Allizard F,Sire JY,Huysseune A

    更新日期:2005-09-01 00:00:00

  • Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine.

    abstract::There is general consensus that enteroendocrine cells, EEC, containing the enteric hormone cholecystokinin (CCK) are confined to the small intestine and predominate in the duodenum and jejunum. Contrary to this, EEC that express the gene for CCK have been isolated from the large intestine of the mouse and there is evi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-017-2612-1

    authors: Fakhry J,Wang J,Martins P,Fothergill LJ,Hunne B,Prieur P,Shulkes A,Rehfeld JF,Callaghan B,Furness JB

    更新日期:2017-08-01 00:00:00

  • Spatial and temporal regulation of glycosylation during Drosophila eye development.

    abstract::Glycosylation plays an essential role during development, in processes such as morphogen distribution, cell-to-cell communication, and extracellular matrix formation. Glycosylation is regulated during development in both a spatial and temporal manner. This study presents a detailed description of glycan distribution f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-009-0753-6

    authors: Yano H,Yamamoto-Hino M,Goto S

    更新日期:2009-04-01 00:00:00

  • Ultrastructural aspects of cryofixed nerves.

    abstract::The ultrastructure of the optic and trigeminal nerves of the rat, cryofixed by use of a liquid nitrogen-propane jet, was examined, paying special attention to the myelin sheath and the cytoskeleton of the axoplasm. The cytoskeleton of the axoplasm is formed by a meshwork of neurofilaments and microtubules connected bo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00214541

    authors: Meller K

    更新日期:1985-01-01 00:00:00

  • Microspectrofluorometric study of monoamines in the auricle of the heart of Protopterus aethiopicus.

    abstract::The auricle of the heart of Protopterus aethiopicus contains large numbers of chromaffin cells, often lying immediately adjacent to the endothelium and displaying a bright blue-white fluorescence characteristic for catecholamines after formaldehyde treatment (Falck and Owman 1965). These results combined with X-ray mi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00233594

    authors: Scheuermann DW,Stilman C,Reinhold C,De Groodt-Lasseel MH

    更新日期:1981-01-01 00:00:00

  • Localization of myosin and actin in ocular nonmuscle cells. Immunofluorescence-microscopic, biochemical, and electron-microscopic studies.

    abstract::Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments,...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00221771

    authors: Drenckhahn D,Gröschel-Stewart U

    更新日期:1977-07-19 00:00:00

  • Immunocytochemical localization of beta-1,3-glucan recognition protein in the silkworm, Bombyx mori.

    abstract::A monospecific antibody against beta-1,3-glucan recognition protein (a 62 kDa protein) of the larval silkworm prophenoloxidase activating system was used to study the localization of the protein. Among tissues from 5th instar larvae, only hemocytes and plasma were shown to contain a 62 kDa polypeptide immunoreactive w...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00319149

    authors: Ochiai M,Niki T,Ashida M

    更新日期:1992-06-01 00:00:00

  • Renewal of the ciliary axoneme in cone outer segments of the retina of Xenopus laevis.

    abstract::The ciliary axoneme in photoreceptors from the retina of Xenopus laevis was examined by immunofluorescent staining of tubulin throughout the light/dark cycle. The immunofluorescent axoneme extended along only part of the length of rod outer segments but the entire length of cone outer segments. Both the cone axoneme a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410050632

    authors: Eckmiller MS

    更新日期:1996-07-01 00:00:00

  • Deleterious effects of intestinal ischemia/reperfusion injury in the mouse enteric nervous system are associated with protein nitrosylation.

    abstract::Changes in intestinal function, notably impaired transit, following ischemia/reperfusion (I/R) injury are likely to derive, at least in part, from damage to the enteric nervous system. Currently, there is a lack of quantitative data and methods on which to base quantitation of changes that occur in enteric neurons. In...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-010-1126-x

    authors: Rivera LR,Thacker M,Pontell L,Cho HJ,Furness JB

    更新日期:2011-04-01 00:00:00

  • The histological architecture of the auditory organs in the parasitoid fly Ormia ochracea.

    abstract::Gravid females of the parasitoid fly species Ormia ochracea (Diptera: Tachinidae) rely on their sense of hearing to detect and localize their hosts, field crickets of the genus Gryllus. As in any hearing insect species, the fly's auditory system consists of a rather complex cascade of mechanical and physiological mech...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s004410000257

    authors: Robert D,Willi U

    更新日期:2000-09-01 00:00:00

  • Ultrastructural changes in contents of membrane-coating granules after extrusion from epithelial cells of hamster cheek pouch.

    abstract::Membrane-coating granules of the epithelium of the hamster cheek pouch displayed a lamellated internal structure consisting of alternating thick and thin electron dense bands separated by translucent bands of equal width. The repeat distance of the contents was 8.5 nm +/- sd. 0.7 nm and the thickness of the major elec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00224374

    authors: Hayward AF

    更新日期:1978-02-24 00:00:00

  • Postnatal development of the brush cells in the common bile duct of the rat.

    abstract::The postnatal development of brush cells in the distal segment of the common bile duct of the rat was examined with respect to cell number and immunoreactivity for liver fatty acid-binding protein (L-FABP). The brush cells, distinguishable from the principal cells by scanning electron microscopy, first appeared in the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00318592

    authors: Iseki S

    更新日期:1991-12-01 00:00:00

  • A novel homozygous nonsense mutation in CAST associated with PLACK syndrome.

    abstract::Peeling skin syndrome is a heterogeneous group of rare disorders. Peeling skin, leukonychia, acral punctate keratoses, cheilitis and knuckle pads (PLACK syndrome, OMIM616295) is a newly described form of PSS with an autosomal recessive mode of inheritance. We report a 5.5-year-old boy with features of PLACK syndrome. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/s00441-019-03077-9

    authors: Temel ŞG,Karakaş B,Şeker Ü,Turkgenç B,Zorlu Ö,Sarıcaoğlu H,Oğur Ç,Kütük Ö,Kelsell DP,Yakıcıer MC

    更新日期:2019-11-01 00:00:00

  • Comparative ultrastructure of ureteric innervation.

    abstract::The distribution and structure of the ureteric nerves in a small series of mammals was compared with that previously demonstrated in the rat. There was marked interspecies variation in the extent to which the nerves penetrated the wall of the ureter and in the degree of development of the deep submucous plexus. In ani...

    journal_title:Cell and tissue research

    pub_type: 杂志文章

    doi:10.1007/BF00225768

    authors: Hoyes AD,Barber P,Martin BG

    更新日期:1975-07-23 00:00:00