Abstract:
:Myosin and actin were localized by indirect immunofluorescence microscopy using specific antibodies prepared in rabbits against highly purified gizzard myosin and actin. A strong fluorescence staining with both antibodies was observed in rat corneal epithelial cells, anterior lens epithelial cells, rod inner segments, and in rat and frog pigment epithelial cells. The immunohistochemical localization of myosin in corneal epithelial cells was further supported by the electrophoretic and immunological identification of smooth muscle type myosin heavy chain in pure corneal epithelial abrasions. Electron-microscopic observations revealed a clear correlation between staining with actin antibodies and the presence of numerous thin cytoplasmic filaments (50-80 A in diameter). The functional and biochemical nature of 90-110 A filaments occurring in corneal and lens epithelial cells, as well as the ultrastructural localization of myosin in ocular nonmuscle cells under study remains obscure.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Drenckhahn D,Gröschel-Stewart Udoi
10.1007/BF00221771subject
Has Abstractpub_date
1977-07-19 00:00:00pages
493-503issue
4eissn
0302-766Xissn
1432-0878journal_volume
181pub_type
杂志文章abstract::Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224731
更新日期:1989-04-01 00:00:00
abstract::The organization of the system of perivascular space around the capillaries in the neurohypophysis was studied in the adult and developing laboratory mouse by the use of histological silver impregnation and electron microscopical techniques. In the median eminence short and long extensions, arising mainly from the sha...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00237730
更新日期:1979-06-08 00:00:00
abstract::We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219075
更新日期:1987-11-01 00:00:00
abstract::Osteoblasts are specialized mesenchyme-derived cells accountable for bone synthesis, remodelling and healing. Differentiation of osteoblasts from mesenchymal stem cells (MSC) towards osteocytes is a multi-step process strictly controlled by various genes, transcription factors and signalling proteins. The aim of this ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-013-1750-3
更新日期:2014-01-01 00:00:00
abstract::The midgut cells of Tomocerus minor (Insecta, Collembola) were examined with the electron microscope and cytochemically. The midgut epithelium consists of a series of cells characterised by numerous mineral concretions scattered throughout the cytoplasm. Mitochondria are abundant; microvilli are well developed at the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236347
更新日期:1979-01-30 00:00:00
abstract::The distribution of cochlin and its associated basement membrane proteins (collagen IV, collagen II, laminin-β2, and nidogen-1) were evaluated in the vestibular endorgans of subjects with Meniere's disease and compared with normal specimens. Cochlin mRNA expression in vestibular endorgans from Meniere's disease specim...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1481-x
更新日期:2012-11-01 00:00:00
abstract::The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5) in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical locali...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217869
更新日期:1984-01-01 00:00:00
abstract::The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particul...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213955
更新日期:1986-01-01 00:00:00
abstract::Colitis is associated with alterations in electrolyte and water transport. These changes give rise to some of the symptoms experienced by patients with colitis. Alterations in fluid flux may also contribute to increased susceptibility to mucosal injury. Recently, endogenous water channel proteins (aquaporins; AQPs), h...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0932-4
更新日期:2004-11-01 00:00:00
abstract::Overexpression of type XIII collagen molecules with an 83-amino-acid residue in-frame deletion of part of the ectodomain leads to fetal lethality in Col13a1COL2del transgenic mice. We characterize here the functional disturbances in the cardiovascular system of mouse fetuses overexpressing mutant type XIII collagen. D...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0617-5
更新日期:2008-07-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) is a major member of a cytokine family with pluripotent biological activity. In the liver, TGF-beta has pathophysiological significance with respect to hepatic fibrogenesis, regulation of liver cell growth, tumor development, and induction of hepatocellular apoptosis. We show...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050740
更新日期:1997-01-01 00:00:00
abstract::Honeybees are extensively used to study olfactory learning and memory processes thanks to their ability to discriminate and remember odors and because of their advantages for optophysiological recordings of the circuits involved in memory and odor perception. There are evidences that the encoding of odors in areas of ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03396-2
更新日期:2021-01-29 00:00:00
abstract::The fine structure of spermatids has been examined in Calliactis, Protanthea, Gonactinia and Parazoanthus (Cnidaria, Anthozoa). The sperm cells are relatively simple and lack distinct acrosomes. Their nuclei, spherical in the zoanthid, in the actinians are slendertipped cones. Condensation of the chromatin is interpre...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222065
更新日期:1975-01-01 00:00:00
abstract::The clear zones seen around microtubules in transverse sections of nutritive tubes vary in size depending on whether a microtubule is bordered by ribosomes or by another microtubule. We consider that such a finding is not consistent with the current view, that the clear zone is maintained by microtubule-associated mat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00204791
更新日期:1982-01-01 00:00:00
abstract::In the present study, we used ultrastructural cytochemistry to analyze the distribution of nuclear and cytoplasmic nucleic acids and polysaccharides, and electron spectroscopic imaging to map the element phosphorus in immature erythroid cells taken from two amphibians, the diploid Bufo ictericus and the tetraploid Odo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00305791
更新日期:1994-10-01 00:00:00
abstract::Polysaccharides in the vacuolar system of an established line of monkey kidney epithelial cells, were investigated by high resolution radioautography following incorporation of 3H-fucose and by the periodic acid-thiosemicarbazide-silver proteinate staining method. Significant amounts of glycogen and glycoproteins were...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225345
更新日期:1978-10-30 00:00:00
abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00209140
更新日期:1978-06-08 00:00:00
abstract::Unfertilized eggs commonly lack centrioles, which are usually provided by the male gamete at fertilization, and are unable to assemble functional reproducing centrosomes. However, some insect species lay eggs that develop to adulthood without a contribution from sperm. We report that the oocyte of the parthenogenetic ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0253-x
更新日期:2006-12-01 00:00:00
abstract::A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-007-0561-9
更新日期:2008-04-01 00:00:00
abstract::The European shore crab Carcinus maenas and the common hermit crab Pagurus bernhardus are members of the sister taxa Brachyura and Anomura (together forming the taxon Meiura) respectively. Both species share similar coastal marine habitats and thus are confronted with similar environmental conditions. This study sets ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1353-4
更新日期:2012-04-01 00:00:00
abstract::Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN + erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitocho...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214924
更新日期:1985-01-01 00:00:00
abstract::Alcohol and aldehyde dehydrogenases (ADHs and ALDHs) may be of interest in the pathology of Parkinson's disease (PD) because of their role in protection against toxins and in retinoid metabolism, which is required for growth and development of the mesencephalic dopamine system. In the present study, the spatial and te...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0038-7
更新日期:2005-11-01 00:00:00
abstract::The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219936
更新日期:1975-12-18 00:00:00
abstract::The antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase were measured in the rete mirabile and gas gland epithelium area of the swim bladder of the toadfish Opsanus tau. When the concentration of enzyme in the swim bladder was compared with the concentration in other organs (kidney, heart, g...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00210458
更新日期:1981-01-01 00:00:00
abstract::Brain injuries trigger physiological reactions which are mediated by a number of cytokines including interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and leukemia inhibitory factor (LIF). Astrocytes and microglia, the protagonists in these traumatic responses, are known to secrete a variety of paracrine signa...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0624-x
更新日期:2002-11-01 00:00:00
abstract::Protein kinase C (PKC) is overexpressed in cancer, including pancreatic cancer, compared with normal tissue. Moreover, PKCα is considered one of the biomarkers for the diagnosis of cancers. In several human cancers, the claudin tight junction molecules are abnormally regulated and are thus promising molecular targets ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-011-1287-2
更新日期:2011-12-01 00:00:00
abstract::The ultrastructural organization of the axons of retinula cells of the eye of the wax moth Galleria mellonella are described. The axons traverse an appreciable distance between the basement membrane of the retina and the lamina ganglionaris of the optic lobe of the brain. The optic tract was reconstructed from serial ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232837
更新日期:1976-11-18 00:00:00
abstract::We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050727
更新日期:1997-01-01 00:00:00
abstract::The kidney is an organ involved in cross talk with many human organs. The link between the immune system and the kidney has been studied in some detail, although data precisely elucidating their interaction are sparse, in particular with regard to the function of the kidney filter apparatus. Current research suggests ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-017-2635-7
更新日期:2017-07-01 00:00:00
abstract::The multicellular epithelial organs in Proteus anguinus, which Bugnion (1873) assumed to be developing neuromasts, have been analyzed by light- and electron-microscopy. Their fundamental structure consists of single ampullae with sensory and accessory cells with apical parts that extend into the pit of the ampulla, an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00217865
更新日期:1984-01-01 00:00:00