Abstract:
:We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells are found to be spherical in shape and, rather than being displaced toward the cell base, occupy a nearly central position in the cytoplasm, even in the storage phase of the secretory cycle. The mucous secretory granules are seen as membrane-limited inclusions, only rarely partially fused to each other. In both mucous and serous cells the Golgi cisterns have numerous large fenestrae which are aligned to form cytoplasmic channels which extend across the stack.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ichikawa M,Ichikawa Adoi
10.1007/BF00219075subject
Has Abstractpub_date
1987-11-01 00:00:00pages
305-14issue
2eissn
0302-766Xissn
1432-0878journal_volume
250pub_type
杂志文章abstract::For the study of the hypothalamo-hypophysial system of Xenopus laevis tadpoles, hypothalamic lesions were made by means of the electrocoagulation technique. Lesioning of the ventral region of the preoptic nucleus resulted in a decrease of the number of ACTH cells in the pars distalis of the pituitary gland and in a di...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00219304
更新日期:1976-06-11 00:00:00
abstract::The epithelial surface of the taste disc on the fungiform papillae of the frog was studied by means of freeze-fracture and conventional electron microscopy. The plasma membrane of the apical process of taste receptor cells was found to exhibit a uniform population of 10-nm intramembrane particles on its fracture face ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216883
更新日期:1982-01-01 00:00:00
abstract::Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a leng...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220391
更新日期:1978-11-20 00:00:00
abstract::The neurosecretory cells of the ventral ganglia in the adult dragonfly, Orthetrum chrysis, are classified into A, B, C1 and C2 cells. The neurosecretory material in the ventral ganglia is composed of PAS-positive material with 1-, 2-glycol groups and some proteins. The proteins rich in cystine or cysteine occur predom...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221087
更新日期:1977-02-09 00:00:00
abstract::The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220184
更新日期:1975-10-13 00:00:00
abstract::Poreless sensilla (np-sensilla) on the antennae of 18 species from 9 insect orders, and on the maxillary palps of Periplaneta americana were investigated using chemo- and cryofixation. The number of np-sensilla is low. Common features of these sensilla are: (a) the presence of a peg, (b) the lack of pores that lead to...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00213769
更新日期:1983-01-01 00:00:00
abstract::Electron-dense granules (EDGs) are singular structures found in the tissues of several vertebrate and invertebrate organisms. Two types of EDGs were observed in hepatopancreatic cells of the crab Ucides cordatus: (1) a non-mineralized EDG, found mainly inside vacuoles, which reacted positively to acid phosphatase and ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-002-0646-4
更新日期:2003-01-01 00:00:00
abstract::Obesity induces metabolic disorders such as type 2 diabetes, hypertension, and cardiovascular diseases and has become a global health concern. Recent studies imply that fat accumulation in nonadipose tissue correlates with metabolic disorders. However, there are no suitable animal models to evaluate this phenomenon. T...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2908-9
更新日期:2019-02-01 00:00:00
abstract::Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224731
更新日期:1989-04-01 00:00:00
abstract::The mammalian airways are known to be richly innervated by several types of peptide-containing nerve fibers. Galanin-containing fibers are, however, comparatively few. The results of the present immunocytochemical study indicate that the chicken airways receive a notably dense supply of galanin-storing fibers. Other m...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00224722
更新日期:1989-04-01 00:00:00
abstract::Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-010-1113-2
更新日期:2011-03-01 00:00:00
abstract::Tight junctions (TJ) between retinal pigmented epithelial (RPE) and retinal endothelial cells maintain the outer and inner blood-retinal barrier, and the breakdown of these barriers is associated with retinal diseases. Vascular endothelial growth factor (VEGF) increases vascular permeability and is thought to be invol...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0046-7
更新日期:2006-01-01 00:00:00
abstract::Desmin is a muscle-specific type III intermediate filament essential for proper muscular structure and function. In human, mutations affecting desmin expression or promoting its aggregation lead to skeletal (desmin-related myopathies), or cardiac (desmin-related cardiomyopathy) phenotypes, or both. Patient muscles dis...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-014-2016-4
更新日期:2015-06-01 00:00:00
abstract::Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiot...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-008-0636-2
更新日期:2008-08-01 00:00:00
abstract::Four different types of spermatogonia were identified in the seminiferous tubules of the Japanese quail: a dark type A (Ad), 2 pale A types (Ap1 and Ap2), and a type B. A model is proposed describing the process of spermatogonial development in the quail. The Ad spermatogonia are considered to be the stem cells. Each ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00319382
更新日期:1992-03-01 00:00:00
abstract::The use of antibodies to alpha-(17--39) ACTH, beta-LPH, and alpha- and beta-endorphins has enabled the detection of immunoreactive cells as early as the eight week of fetal life. Immunoreactive cells are located in the anterior and intermediate lobes of the pituitary gland in normal human fetuses. The same cells react...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00225339
更新日期:1978-10-30 00:00:00
abstract::Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the ne...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215442
更新日期:1988-01-01 00:00:00
abstract::Structures identified as subsurface cisterns (SSC's) were found in neurons of the paraventricular nuclei of the rat hypothalamus. They appeared as cytoplasmic organelles consisting most often of stacks of parallel cisterns apposed to the neuronal plasmalemma. These SSC's were located in the interneurons of the parvoce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236138
更新日期:1979-06-27 00:00:00
abstract::N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214676
更新日期:1987-10-01 00:00:00
abstract::Neural stem cells (NSCs) are a subtype of tissue-specific progenitor cells capable of extended self-renewal and the ability to generate all major cell types of nervous tissue, such as neurons, astroglia and oligodendroglial cells. Recent studies suggest that salient patterning in anterior-posterior and dorsal-ventral ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-004-0923-5
更新日期:2004-10-01 00:00:00
abstract::Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats,...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051119
更新日期:1998-08-01 00:00:00
abstract::Our immunocytochemical investigation of the magnocellular neuroendocrine system in the goldfish hypothalamus reveals enkephalin (ENK)-containing neurons interspersed among the vasotocin (VT)- and isotocin (IT)-containing neurons of the preoptic nucleus. The perikarya of the ENK, VT, and IT neurons do not show distinct...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00236894
更新日期:1979-08-03 00:00:00
abstract::Nitric oxide (NO) stimulates osteoblast differentiation, but whether NO contributes to odontoblast differentiation during dentin repair is unknown. By using reverse transcription/polymerase chain reaction and immunostaining, we investigated the gene expression and/or immunolocalization of endothelial NO synthase (eNOS...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0003-5
更新日期:2007-04-01 00:00:00
abstract::The pinealocytes of the pig contain conspicuous dense bodies, the nature and role of which are not yet fully elucidated. The aim of this study was to demonstrate whether or not these structures are involved in the secretion process. The tannic acid-Ringer incubation (TARI)-method, which allows a clear-cut ultrastructu...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00313977
更新日期:1991-05-01 00:00:00
abstract::Carotid bodies of ducks show no detectable changes in ultrastructure up to periods of four weeks following the removal of 2-3 cm of the cervical vagus (decentralization). This indicates that the majority, and possibily all the nerves terminating on the glomus cells are afferent (sensory) in nature. These nerve endings...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218494
更新日期:1975-11-19 00:00:00
abstract::The present immunocytochemical study concerns the distribution of four neuropeptides, FMRF-amide, vasotocin, leu-enkephalin and neurotensin, and of the bioamine serotonin in the plerocercoid larva of Diphyllobothrium dendriticum. Anti-FMRF-amide and vasotocin-reactivity occurs in perikarya and nerve fibres in the CNS ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222332
更新日期:1985-01-01 00:00:00
abstract::Neurons projecting from the brain to the pineal gland via the pineal stalk were investigated in the golden hamster with the use of the retrograde horseradish-peroxidase tracing method both in vivo and in vitro. Labelled perikarya were observed in the medial and lateral habenular nuclei as well as in the posterior comm...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00216557
更新日期:1987-01-01 00:00:00
abstract::Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell population...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0282-5
更新日期:2007-01-01 00:00:00
abstract::Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2931-x
更新日期:2019-03-01 00:00:00
abstract::In an ultrastructural study on the spermiogenesis of the rainbow trout (Salmo gairdneri R.) four spermatogenetic stages were identified. In young round spermatids, the nuclear chromatin was first heterogeneous (euchromatin and heterochromatin). Subsequently, it became more homogeneous and started to condense in the fo...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00238295
更新日期:1983-01-01 00:00:00