The fine structure of sublingual gland acinar cells of the Mongolian gerbil, Meriones unguiculatus, processed by rapid freezing followed by freeze-substitution fixation.

Abstract:

:We compare the ultrastructure of the gerbil sublingual gland as seen after cryofixation followed by substitution with osmium tetroxide, with the more familiar appearance of material processed by glutaraldehyde-osmium chemical fixation. After primary cryofixation of fresh salivary glands, the nuclei of the mucous cells are found to be spherical in shape and, rather than being displaced toward the cell base, occupy a nearly central position in the cytoplasm, even in the storage phase of the secretory cycle. The mucous secretory granules are seen as membrane-limited inclusions, only rarely partially fused to each other. In both mucous and serous cells the Golgi cisterns have numerous large fenestrae which are aligned to form cytoplasmic channels which extend across the stack.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Ichikawa M,Ichikawa A

doi

10.1007/BF00219075

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

305-14

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

250

pub_type

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