Abstract：
:Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with α-, β- and δ-crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN's status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
García Caballero G,Schmidt S,Schnölzer M,Schlötzer-Schrehardt U,Knospe C,Ludwig AK,Manning JC,Muschler P,Kaltner H,Kopitz J,Gabius HJdoi
10.1007/s00441-018-2931-xsubject
Has Abstractpub_date
2019-03-01 00:00:00pages
665-683issue
3eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-018-2931-xjournal_volume
375pub_type
杂志文章abstract：:Neuroblastoma is an embryonal malignancy that affects normal development of the adrenal medulla and paravertebral sympathetic ganglia in early childhood. Extensive studies have revealed the molecular characteristics of human neuroblastomas, including abnormalities at genome, epigenome and transcriptome levels. However...
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