Abstract：
:Previous studies have indicated the importance of basement membrane components both for cellular differentiation in general and for the barrier properties of cerebral microvascular endothelial cells in particular. Therefore, we have examined the expression of basement membrane proteins in primary capillary endothelial cell cultures from adult porcine brain. By indirect immunofluorescence, we could detect type IV collagen, fibronectin, and laminin both in vivo (basal lamina of cerebral capillaries) and in vitro (primary culture of cerebral capillary endothelial cells). In culture, these proteins were secreted at the subcellular matrix. Moreover, the interaction between basement membrane constituents and cerebral capillary endothelial cells was studied in adhesion assays. Type IV collagen, fibronectin, and laminin proved to be good adhesive substrata for these cells. Although the number of adherent cells did not differ significantly between the individual proteins, spreading on fibronectin was more pronounced than on type IV collagen or laminin. Our results suggest that type IV collagen, fibronectin, and laminin are not only major components of the cerebral microvascular basal lamina, but also assemble into a protein network, which resembles basement membrane, in cerebral capillary endothelial cell cultures.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Tilling T,Engelbertz C,Decker S,Korte D,Hüwel S,Galla HJdoi
10.1007/s00441-002-0604-1keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
19-29issue
1eissn
0302-766Xissn
1432-0878journal_volume
310pub_type
杂志文章abstract：:In order to compare the functional state of the anterior pituitary of spontaneously hypertensive rats (SHR) with that of nonmotensive Wistar Kyoto rats (WKR), the anterior pituitary was examined by morphometry and autoradiography at the level of electron microscopy. The relative number and the relative volume of thyro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237005
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abstract：:Periodontal ligament (PDL) stem cell properties are critical in the periodontal tissue regeneration for periodontitis. Previously, we have demonstrated that cigarette smoking attenuates PDL-derived stem cell (PDLSC) regenerative properties. Here, we report the findings on the regenerative properties of human PDLSCs wi...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237813
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
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更新日期：2001-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214368
更新日期：1988-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-011-1227-1
更新日期：2012-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2011-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221652
更新日期：1989-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232837
更新日期：1976-11-18 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210521
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216767
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216024
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1994-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318486
更新日期：1995-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222194
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1994-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221773
更新日期：1977-07-19 00:00:00
