Abstract：
:Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug. In the distal elongating tips of myotubes, microtubules were the major cytoskeletal elements; actin and intermediate filaments were much less abundant. On the other hand, colcemid- and nocodozole-treatments caused disruption of microtubles and also prompt retraction of growth tips to form myosacs, a type of deformed myotube. Actin filaments remained unaffected during the retraction. The difference in the distribution of the 3 cytoskeletal filaments in the region of growth tips was most remarkable in the case of those myotubes in the process of recovery from myosacs. In an early phase of recovery, the cellular processes extending from myosacs were enriched with both microtubules and intermediate filaments, but not with actin filaments. Later, when the processes became further developed, intermediate filaments were scarce at the extreme ends. Fluorescein-labeled actin introduced by a micro-injection method was minimally incorporated into filaments in the cellular processes. We conclude that microtubules make up the cytoskeletal element which is most responsible for elongation or spreading of growth tips of myotubes in vitro.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Saitoh O,Arai T,Obinata Tdoi
10.1007/BF00214368subject
Has Abstractpub_date
1988-05-01 00:00:00pages
263-73issue
2eissn
0302-766Xissn
1432-0878journal_volume
252pub_type
杂志文章abstract：:The chemical composition of intermediate filaments (IF's) in the ependyma of the subcommissural organ (SCO) of the Mongolian gerbil (Meriones unguiculatus) was investigated immunohistochemically in paraffin-embedded tissue. Antibodies against glial fibrillary acidic protein (GFAP), vimentin, neurofilament proteins and...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218796
更新日期：1989-03-01 00:00:00
abstract：:Enteroendocrine cells containing glucagon-, substance P-, neurotensin- and VIP-like substances have been demonstrated immunocytochemically in the gut of Barbus conchonius. Mainly based on the distribution of the immunoreactive endocrine cells in this and a previous study, at least eight different enteroendocrine cell ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229200
更新日期：1984-01-01 00:00:00
abstract：:We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050727
更新日期：1997-01-01 00:00:00
abstract：:The expression of corticosteroids and regional expression 16 (CR16) and neural Wiscott-Aldrich syndrome protein (N-WASP) was studied in the testes of men with idiopathic azoospermia by (1) immunohistochemistry and Western blot to detect the expression of CR16 and N-WASP protein, (2) reverse transcription with the poly...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1159-9
更新日期：2011-06-01 00:00:00
abstract：:The octavo-lateral efferent system of several anuran species was studied by means of retrograde transport of horseradish peroxidase. This system is organized similarly in all larval anurans and in all adult aglossids. All have two groups of efferent neurons in the nucleus reticularis medialis between the VIIIth and th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214812
更新日期：1982-01-01 00:00:00
abstract：:This work describes the origin and development of about 200 tension receptor cells located around the anterior attachment site of the locust ovipositor muscle and their migration to their final position on the muscle fibres. The locust ovipositor muscle is the only insect system in which more than 100 tension receptor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0498-z
更新日期：2007-12-01 00:00:00
abstract：:Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in ti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0688-7
更新日期：2003-03-01 00:00:00
abstract：:Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1113-2
更新日期：2011-03-01 00:00:00
abstract：:Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0596-6
更新日期：2008-06-01 00:00:00
abstract：:We develop "autologous bone marrow cell infusion (ABMi) therapy" for the treatment of human decompensated liver cirrhosis and confirm the efficacy and safety of this treatment in multicenter clinical studies. With the goal of further expanding the applications of ABMi, we first cultured human bone marrow cells and the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1727-2
更新日期：2013-12-01 00:00:00
abstract：:Neuroblastoma cells grown on substrates in culture develop long processes and assume the morphology of normal neurons as judged light microscopically. The development of synapses in the cultured tissue is studied by periodic electron microscopic examination of the areas of contact between cells. The initial explants a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232282
更新日期：1980-01-01 00:00:00
abstract：:The protein tyrosine phosphatase interacting protein 51 (PTPIP51) is thought to regulate crucial cellular functions such as mitosis, apoptosis, migration, differentiation and communication between organelles as a scaffold protein. These diverse functions are modulated by the tyrosine/serine phosphorylation status of P...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-016-2508-5
更新日期：2017-06-01 00:00:00
abstract：:In situ hybridisation and immunohistochemistry analyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFalpha), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0993-5
更新日期：2010-08-01 00:00:00
abstract：:The orderly arrangement of fibroblasts and collagen in tendons and ligaments suggests that these cells may have precise relationships with one another and with the collagen fibrils. The spatial organization of rat tail tendon was therefore examined using scanning and transmission electron microscopy and by reconstruct...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217304
更新日期：1984-01-01 00:00:00
abstract：:Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0668-7
更新日期：2008-10-01 00:00:00
abstract：:Nerve growth factor (NGF) induces morphological and physiological changes in cultured pancreatic beta-cells, including the extension of neurite-like processes. This latter effect is potentiated by dibutyryl cAMP (dbcAMP). beta-cells cultured under these conditions maintain their immunoreactivity to insulin and gamma-a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100411
更新日期：2001-09-01 00:00:00
abstract：:Ornithine decarboxylase (ODC) activity was measured in homogenates of neonatal rabbit ovaries from the day of birth to day 22, an interval that corresponds to the progressing stages of the meiotic prophase from oogonia to diplotene and follicle formation. The ODC-activity was high on days 1 and 3 when most germ cells ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218114
更新日期：1983-01-01 00:00:00
abstract：:The distribution and abundance of nitric oxide synthase (NOS)-containing neurons and their terminals in the gastrointestinal tract of the guinea-pig were examined in detail using NADPH diaphorase histochemistry and NOS immunohistochemistry. NOS-containing cell bodies were found in the myenteric plexus throughout the g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00303090
更新日期：1994-07-01 00:00:00
abstract：:S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00313528
更新日期：1990-09-01 00:00:00
abstract：:Progenitor stem cells have been identified, isolated and characterized in numerous tissues and organs. However, their therapeutic potential and the use of these stem cells remain elusive except for a few progenitor cells from bone marrow, umbilical cord blood, eyes and dental pulp. The use of bone marrow-derived hemat...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1471-z
更新日期：2012-10-01 00:00:00
abstract：:Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer's patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such prote...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2342-1
更新日期：2016-06-01 00:00:00
abstract：:The posterior rhombencephalic tela choroidea of the bullfrog was examined by electron microscopy. This membrane, the pia-ependymal roof of the caudal hindbrain, contains a large central region characterized by cuboidal ependymal cells which surround sizable microscopic apertures -- the interependymal pores. Ultrastruc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220439
更新日期：1979-11-01 00:00:00
abstract：:Semaphorin 3A (Sema3A) axon repellant serves multiple developmental functions. Sema3A mRNAs are expressed in epithelial and mesenchymal components of the developing incisor in a dynamic manner. Here, we investigate the functions of Sema3A during development of incisors using Sema3A-deficient mice. We analyze histomorp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1839-3
更新日期：2014-07-01 00:00:00
abstract：:Orexins (OxA and OxB) and their receptors (Ox1R and Ox2R), originally detected in the hypothalamus, have also been localized in multiple cerebral areas and peripheral organs. Thus, in addition to their central function in the regulation of food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual beha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1394-8
更新日期：2012-06-01 00:00:00
abstract：:This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other musc...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-005-0019-x
更新日期：2005-11-01 00:00:00
abstract：:A quantitative evaluation of putative amino acid neurotransmitters in sea pansy polyps by high-performance liquid chromatography indicates that the taurine content exceeds that of other amino acids by a 100-fold. The cellular source of this taurine was investigated by immunohistochemistry with two polyclonal antisera ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050800
更新日期：1997-04-01 00:00:00
abstract：:The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and ele...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050986
更新日期：1998-01-01 00:00:00
abstract：:The influence of adrenalectomy and administration of hypertonic saline on the amount of vasopressin, oxytocin, and neurophysin contained in the median eminence and the neural lobe of rats was studied by means of the following methods: (i) morphometric and microphotometric analyses of aldehyde fuchsin-stained histologi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00206271
更新日期：1983-01-01 00:00:00
abstract：:The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219936
更新日期：1975-12-18 00:00:00
abstract：:The antennal lobe is the primary processing center for olfactory information in insects. To understand further the neural circuitry of this brain area, we have investigated the distribution of gamma-aminobutyric acid (GABA) and its colocalization with neuropeptides in the antennal lobe of the noctuid moth Heliothis vi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0744-z
更新日期：2009-03-01 00:00:00
