Abstract：
:In the present study, we used ultrastructural cytochemistry to analyze the distribution of nuclear and cytoplasmic nucleic acids and polysaccharides, and electron spectroscopic imaging to map the element phosphorus in immature erythroid cells taken from two amphibians, the diploid Bufo ictericus and the tetraploid Odontophrynus americanus. In the cytoplasm of cells from the tetraploid species, we detected numerous inclusions containing a material that was similar to the dispersed chromatin seen in the nucleus of these cells. The RNase-gold complex labeled both the dispersed nuclear chromatin and the cytoplasmic inclusions. The Thiéry technique showed that glycoconjugates were present in all the membranous complexes of the erythroid cells of both types of amphibians under study, although they were absent within or around the cytoplasmic RNA inclusions. Electron spectroscopic imaging revealed the presence of phosphorus in these inclusions. These data suggest that an increase in RNA synthesis occurs in tetraploid amphibian cells, probably as a result of an alteration in the mechanisms of gene regulation.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Cianciarullo AM,Meirelles Mde Ndoi
10.1007/BF00305791subject
Has Abstractpub_date
1994-10-01 00:00:00pages
187-95issue
1eissn
0302-766Xissn
1432-0878journal_volume
278pub_type
杂志文章abstract：:Four forms of short neuropeptide F (sNPF1-4), derived from the gene snpf, have been identified in Drosophila and are known to act on a single G-protein-coupled receptor (sNPFR). Several functions have been suggested for sNPFs in Drosophila, including the regulation of feeding and growth in larvae, the control of insul...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1660-4
更新日期：2013-09-01 00:00:00
abstract：:Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410051072
更新日期：1998-06-01 00:00:00
abstract：:Fueled by the discovery of head regeneration in triclads (planarians) two and a half centuries ago, flatworms have been the focus of regeneration research. But not all flatworms can regenerate equally well and to obtain a better picture of the characteristics and evolution of regeneration in flatworms other than plana...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03302-w
更新日期：2020-11-07 00:00:00
abstract：:Dexamethasone (Dex) regulates osteoblastic and adipocytic differentiation in mesenchymal progenitor cells through regulation of Wnt/β-catenin signaling. To elucidate the regulatory mechanisms underlying the effects of Dex, we examine the expression of Axin2, which is an intracellular inhibitor of Wnt/β-catenin signali...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1696-5
更新日期：2013-12-01 00:00:00
abstract：:The fine structure of the ventrolateral and dorsomedial subdivisions of the ventromedial nucleus (VMN) of the hypothalamus was examined in ovariectomized/control and ovariectomized/estrogen-treated rats to compare neurons of these areas to other neurons (specifically the ventrolateral thalamus), and to determine the e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219357
更新日期：1981-01-01 00:00:00
abstract：:The ontogeny of gut innervation in the anuran amphibian Xenopus laevis was studied using immunohistochemistry on sections of whole larvae from NF stages 38-52. Immunoreactivity to acetylated tubulin confirmed the presence of nerve fibres as early as stages 38-39. Actin immunoreactivity was found at stage 41, indicatin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100423
更新日期：2001-10-01 00:00:00
abstract：:Radiofrequency lesions were made in the lateral geniculate nuclei of six squirrel monkeys. The resulting degenerating terminals and their postsynaptic structures in layer IV of area 17 were quantitatively categorized on photomontages covering large areas of neuropil. Two to five days after the lesion, numerous axon te...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234741
更新日期：1979-02-28 00:00:00
abstract：:PC12 pheochromocytoma cells treated with nerve growth factor (NGF) in combination with high concentrations of the activators of adenylate cyclase, forskolin or cholera toxin, become more neuron-like in size than cells treated with NGF or with activators of adenylate cyclase alone. Cells treated simultaneously with NGF...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215430
更新日期：1987-07-01 00:00:00
abstract：:It is possible to determine statistically the repartitions of shapes or sizes in a population of particles from the recurrence of shapes or sizes of their outlines in microscopical sections. This method was applied to a population of 1326 follicular profiles randomly sampled from 30 ovaries of pubescent homozygous "Nu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219416
更新日期：1976-07-30 00:00:00
abstract：:Dorsal unpaired median (DUM) neurones in the abdominal ganglia of the locust were impaled with microelectrodes and some were injected intracellularly with horseradish peroxidase so that their synapses could be identified in the electron microscope. Simultaneous recordings from DUM neurones in different abdominal gangl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307805
更新日期：1995-05-01 00:00:00
abstract：:Electron-microscopic evidence indicates that during conditions of high hormone demand such as lactation there is a dramatic reduction in the number of fine glial processes which are normally interposed between magnocellular neuroendocrine cell somata in the supraoptic nucleus (SON). The purpose of this study was to co...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225557
更新日期：1985-01-01 00:00:00
abstract：:Immunocytochemistry was used to determine if photoperiod and/or sex have any effect on the pattern of the luteinizing hormone-releasing hormone (LHRH) system in the brain of the Japanese quail. Immunopositive perikarya were found within three major areas of the brain: the rostral paraolfactory lobe, the preoptic, and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222289
更新日期：1988-08-01 00:00:00
abstract：:In the atherosclerotic plaque, smooth muscle cells (SMC) acquire an inflammatory phenotype. Resistin and fractalkine (CX3CL1) are found in human atheroma and not in normal arteries. CX3CL1 and CX3CR1 are predominately associated with SMC. We have questioned whether resistin has a role in the expression of CX3CL1 and C...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1510-9
更新日期：2013-01-01 00:00:00
abstract：:Neural stem cells (NSCs) are a subtype of tissue-specific progenitor cells capable of extended self-renewal and the ability to generate all major cell types of nervous tissue, such as neurons, astroglia and oligodendroglial cells. Recent studies suggest that salient patterning in anterior-posterior and dorsal-ventral ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-004-0923-5
更新日期：2004-10-01 00:00:00
abstract：:The effect of hypothyroidism, induced by 4-propyl-2-thiouracil, on muscle satellite cells in vivo and in vitro, and on postnatal muscle fiber development in the soleus muscle of rats during the first 40 days of postnatal life was analyzed. The proliferative activity of satellite cells was determined by means of bromod...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050682
更新日期：1996-10-01 00:00:00
abstract：:Morphological changes in the disconnected neuro-intermediate lobe were studied in the lizard, Anolis carolinensis from the 2nd to the 14th post-operative day using a threefold aldehyde fixative (Rodríguez, 1969). Two phases of colour change capacity were exhibited: Phase I started immediately after the transection, la...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237724
更新日期：1979-06-08 00:00:00
abstract：:The interstitial cells of Cajal (ICCs) are important mediators of gastrointestinal (GI) motility because of their role as pacemakers in the GI tract. In addition to their function, ICCs are also structurally distinct cells most easily identified by their ultra-structural features and expression of the tyrosine kinase ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1434-4
更新日期：2012-08-01 00:00:00
abstract：:The three-dimensional architecture of the lamina propria in the jejunal mucosa of the rat was studied by scanning electron microscopy. The lamina propria is a typical reticular tissue with fine nets of reticular fibers and free and fixed cells lying among them. However, the lamina propria in the core of villi displaye...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225563
更新日期：1985-01-01 00:00:00
abstract：:Seasonal changes have been detected in the ultrastructure of brown adipose tissue (BAT) of the common shrew, Sorex araneus (a true nonhibernator) living under natural conditions and collected at the time when the most representative growth phase of the animal for the given season could be expected. In summer and autum...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233501
更新日期：1981-01-01 00:00:00
abstract：:In an organ culture system under a three-dimensional microenvironment that provides the conditions needed for odontoblast differentiation, a row of odontoblasts can be induced (Kikuchi et al. 1996, 2001). Therefore, in a newly designed three-dimensional cell culture system that fulfils the conditions necessary for odo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0882-x
更新日期：2004-08-01 00:00:00
abstract：:Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213735
更新日期：1984-01-01 00:00:00
abstract：:Ontogenetic development of GRF-containing neurons in the rat hypothalamus was studied employing antisera which were generated against hpGRF (1-44)NH2 and rhGRF(1-43)OH: anti-hpGRF-C and -rhGRF sera recognize the species-specific C-terminal portions of the peptides, and anti-hpGRF-MC and -N sera recognize hpGRF(27-44)N...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225416
更新日期：1985-01-01 00:00:00
abstract：:During the reproductive period of the male stickleback, structural and functional changes of the kidney take place, both in the glomeruli and in the renal tubule cells. The structural changes in the glomeruli involve almost all glomerular components and point to a reduction of the glomerular filtration rate. The purpo...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210146
更新日期：1981-01-01 00:00:00
abstract：:The pituitary gland is first evident in rainbow trout two weeks before hatching. Differentiation of prolactin and ACTH cells is not marked until 3-4 days after hatching when the follicular arrangement of the prolactin cells become apparent. There is no difference in the time of development of either prolactin or ACTH ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209283
更新日期：1978-05-29 00:00:00
abstract：:Bone morphogenetic proteins (BMPs) induce cartilage and bone formation at both bony and non-bony sites. We examined the possibility whether BMP-2 induces differentiation of osteoblast progenitors into chondroblast lineage cells using organ culture and cell culture prepared from the calvaria of newborn mouse. BMP-2 sti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050562
更新日期：1996-04-01 00:00:00
abstract：:Cyclooxygenase-2 (COX-2) is a recently discovered isoform of cyclooxygenase that is inducible by various types of inflammatory stimuli. Although this enzyme is considered to play a major role in inflammation processes by catalyzing the production of prostaglandins, the precise location, distribution, and regulation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000296
更新日期：2001-02-01 00:00:00
abstract：:Interstitial cells in the pineal gland of the rat were characterized immunocytochemically using the monoclonal antibodies MRC OX-42 and ED1 for macrophages/microglia, and MRC OX-6, which recognizes major histocompatibility complex (MHC) class II antigen. A polyclonal antibody against GFAP was used to identify astrocyt...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302731
更新日期：1993-05-01 00:00:00
abstract：:To gain a better understanding of the neuroplasticity of sympathetic neurons during postnatal ontogenesis, the distribution of neuronal nitric oxide synthase (nNOS) immunoreactivity was studied in sympathetic preganglionic neurons (SPN) in the spinal cord (Th2 segment) of female Wistar rats at different ages (newborn,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2925-8
更新日期：2019-02-01 00:00:00
abstract：:The cardiovascular system is the first functional organ in the vertebrate embryo, and many organs start to develop adjacent to cells of the cardiovascular system. Endothelial cells (EC) form the inner cell lining of blood vessels and represent the major cell type that interacts with developing organs. On the one hand,...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-003-0739-8
更新日期：2003-10-01 00:00:00
abstract：:Ex-fissiparous planarians produce infertile cocoons or, in very rare cases, cocoons with very low fertility. Here, we describe the features of programmed cell death (PCD) occurring in the hyperplasic ovary of the ex-fissiparous freshwater planarian Dugesia arabica that may explain this infertility. Based on TEM result...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1971-0
更新日期：2014-11-01 00:00:00
