GABA and glutamate-like immunoreactivity at synapses received by dorsal unpaired median neurones in the abdominal nerve cord of the locust.

Abstract:

:Dorsal unpaired median (DUM) neurones in the abdominal ganglia of the locust were impaled with microelectrodes and some were injected intracellularly with horseradish peroxidase so that their synapses could be identified in the electron microscope. Simultaneous recordings from DUM neurones in different abdominal ganglia revealed that they received common postsynaptic potentials from descending interneurones. Post-embedding immunocytochemistry using antibodies against GABA and glutamate was carried out on ganglia containing HRP-stained neurones. GABA-like immunoreactivity was found in 39% (n = 82) of processes presynaptic to abdominal DUM neurones and glutamate-like immunoreactivity in 21% (n = 42) of presynaptic processes. Output synapses from the DUM neurites were rarely observed within the neuropile. Structures resembling presynaptic dense bars but not associated with synaptic vesicles, were seen in some large diameter neurites.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Pflüger HJ,Watson AH

doi

10.1007/BF00307805

subject

Has Abstract

pub_date

1995-05-01 00:00:00

pages

325-33

issue

2

eissn

0302-766X

issn

1432-0878

journal_volume

280

pub_type

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