Abstract：
:The intestine, which is exposed to nutrition and to food-derived antigens and microbes including viruses and bacteria, might be an important site for the immune response. Crucial structural and functional differences exist between the small and large intestine, regional differences even having been demonstrated within the small intestine. Accordingly, intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) might be heterogeneous among the different intestinal regions. The aim of this study has been to describe, as accurately as possible, the numbers and T-cell receptor (TCR) phenotypes of IELs and LPLs present in distinct regions of the murine small intestine under physiological conditions. Using an immunohistological technique to differentiate IELs from LPLs, the differential enumeration of IELs and LPLs in distinct regions of the murine small intestine, based upon their definition originally determined by their location, has been performed for the first time and has demonstrated that (1) there are more IELs than LPLs in the duodenum and jejunum, but more LPLs than IELs in the ileum, (2) in the duodenum and jejunum, TCRgammadelta IELs account for 70%-75% of the total CD3(+) IELs, a much greater percentage than previously reported, (3) the ratio of TCRgammadelta to TCRalphabeta IELs is inverted in the ileum, with more than 75% IELs being TCRalphabeta-positive, (4) the lamina propria forms one functional unit throughout the small intestine in terms of the TCR subset components (TCRalphabeta:TCRgammadelta=3:1), and (5) the ileum is entirely different from other regions of the small intestine. To deepen our understanding of the functional significance of the small intestine as an immunologically competent organ, the precise distributions of IELs and LPLs, the ratio of their various subsets, and the strict distinction of IELs and LPLs, as described in this study, is indispensable.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Tamura A,Soga H,Yaguchi K,Yamagishi M,Toyota T,Sato J,Oka Y,Itoh Tdoi
10.1007/s00441-003-0706-4keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
47-53issue
1eissn
0302-766Xissn
1432-0878journal_volume
313pub_type
杂志文章abstract：:The spatial localisation of insulin-like growth-factor-binding protein-2 (IGFBP-2) and its mRNA was investigated during larval and post-larval developmental stages of the gilthead seabream ( Sparus aurata) by immunohistochemistry and in situ hybridisation with specific antisera and riboprobes. During larval developmen...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0952-0
更新日期：2005-01-01 00:00:00
abstract：:Mytilus mussels are characterized by annually repeated reproduction which is associated with subsequent growth, morphogenesis, breakdown and redevelopment of the gonad and reproductive tract into mantle mesenchyme. We present a description of the expression of the male-associated polypeptide (MAP; see Mikhailov et al....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051204
更新日期：1998-12-01 00:00:00
abstract：:Despite the common structure of vertebrates, the development of the vertebral column differs widely between teleosts and tetrapods in several respects, including the ossification of the centrum and the function of the notochord. In contrast to tetrapods, vertebral development in teleosts is not fully understood, parti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2404-z
更新日期：2016-10-01 00:00:00
abstract：:We have recently reported that Sox2-expressing pituitary stem/progenitor cells contact each other via a tight-junction protein CAR to form stem/progenitor cell niches in the marginal cell layer facing the lumen and in the clusters scattered in the parenchyma of the anterior lobe. However, the microenvironment of the n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2054-y
更新日期：2015-03-01 00:00:00
abstract：:Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224303
更新日期：1976-07-26 00:00:00
abstract：:Their strategic location between blood and tissue and their constitutive properties allow endothelial cells (EC) to monitor the transport of plasma molecules, by employing bidirectional receptor-mediated and receptor-independent transcytosis and endocytosis, and to regulate vascular tone, cellular cholesterol and lipi...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0678-5
更新日期：2009-01-01 00:00:00
abstract：:Immunoreactivity for calcitonin gene-related peptide is demonstrated for the first time in neuroepithelial bodies in the lung of newborn cats after Bouin fixation and embedding in paraffin. The intense staining clearly identifies these bodies at the level of bronchioli and alveoli. Occasionally, single neuroepithelial...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215517
更新日期：1987-08-01 00:00:00
abstract：:Neurotrophins signal via a dual-receptor system comprising receptor tyrosine kinases, the Trks, and a tumor necrosis factor (TNF) receptor like molecule, p75. Interest in these receptors was spurred on by the finding that they are employed by their neurotrophin ligands to activate opposing cellular mechanisms. Signall...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410100378
更新日期：2001-08-01 00:00:00
abstract：:Endothelial cells separate the intra- and extravascular space and regulate transport processes between these compartments. Since intercellular junctions are required for these specific cell functions, the cell-cell contacts in the permanent cell line ECV304 were systematically analyzed and compared with human umbilica...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051340
更新日期：1999-07-01 00:00:00
abstract：:The notion that teeth contain stem cells is based on the well-known repairing ability of dentin after injury. Dental stem cells have been isolated according to their anatomical locations, colony-forming ability, expression of stem cell markers, and regeneration of pulp/dentin structures in vivo. These dental-derived s...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0467-6
更新日期：2008-01-01 00:00:00
abstract：:Dome epithelium (DE), the tissue covering lymphoid domes of gut-associated lymphoid tissues, was examined in both adult and neonatal rabbit appendix or sacculus rotundus to determine if dome epithelial cells matured earlier than epithelial cells covering adjacent villi. The localization of well-differentiated epitheli...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216473
更新日期：1987-06-01 00:00:00
abstract：:Effect of colchicine on the ultrastructure of taste bud cells was studied in the mouse. In untreated mice microtubules were abundant throughout the entire cytoplasm of type-III cells, but only in the apical cytoplasm of type-I cells. After 2 h of colchicine treatment, no microtubules were observed in any taste bud cel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214555
更新日期：1985-01-01 00:00:00
abstract：:The glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) comprise a group of four homologous and potent growth factors that includes GDNF, neurturin (NRTN), artemin (ARTN), and persephin (PSPN). The survival, growth, and mitotic activities of the GFLs are conveyed by a single receptor tyrosine kina...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03235-4
更新日期：2020-10-01 00:00:00
abstract：:The olfactory epithelium of mice after axotomy was investigated to clarify the stem cells of olfactory cells by double immunostaining using antikeratin (MA903) and anti-bromodeoxyuridine (BrdU) antibodies and by conventional electron microscopy. When a single dose of BrdU was given to mice 9 days after axotomy, immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318179
更新日期：1991-11-01 00:00:00
abstract：:Although Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dent...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0655-z
更新日期：2008-09-01 00:00:00
abstract：:To study the biosynthesis and intracellular migration of glycoproteins in the adrenal gland, adult mice were injected intravenously with L-(3H)-fucose and killed from 10 min to 14 days after injection. Semi-thin sections of the adrenal glands were then processed for radioautography. Incorporation of labeled fucose occ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232246
更新日期：1979-11-01 00:00:00
abstract：:The fine structure, nature and fate of the components of the nucleolus were studied in young (steps 1, 2), intermediate (steps 3, 4, 5) and mature spermatids (steps 6, 7, 8) of man and monkey, by use of several cytochemical techniques (alcoholic PTA; sodium tungstate: EDTA; HAPTA; nuclease-gold complexes; NOR silver s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00305735
更新日期：1991-04-01 00:00:00
abstract：:This study investigates the relationship between pituitary LHRH responsiveness and the depletion of LH in pubertal rats. The anterior pituitaries of 7-week-old rats of both sexes were stimulated for a maximum of 24 h with either a continuous, or pulsatile exposure to LHRH in vitro. Immunohistochemical examination reve...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218057
更新日期：1986-01-01 00:00:00
abstract：:An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone ahd no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216742
更新日期：1981-01-01 00:00:00
abstract：:In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of the major Cx32 protein is downregulated during liver regeneration and cholestasis. Here we have analyzed the acute-phase response (after experimental inflammation) an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000177
更新日期：2000-04-01 00:00:00
abstract：:The insect midgut epithelium represents an interface between the internal and the external environment and it is the almost unique epithelial tissue by which these arthropods acquire nutrients. This epithelium is indeed able to produce digestive enzymes and to support vectorial transport of small organic nutrients, io...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-019-03076-w
更新日期：2019-09-01 00:00:00
abstract：:The technique of cobalt sulfide precipitation combined with Timm's sulfide-silver method for intensification of heavy metals was used to delineate the retino-hypothalamic projection of the rat. Freshly isolated rat brains were dissected and a solution of cobaltous chloride was applied to one of the cut optic nerves. S...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219728
更新日期：1976-04-28 00:00:00
abstract：:Colonies of the ascidian Botryllus schlosseri undergo recurrent generation changes in which adult zooids are gradually resorbed and replaced by new blastogenic generations. During these periods, known as take-over phases, programmed cell death, which, on the basis of morphological analysis is ascribed to apoptosis, oc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0738-9
更新日期：2003-06-01 00:00:00
abstract：:The distribution and ultrastructure of the mitochondria-rich (MR) cells in the gills of larval (ammocoetes) and adult lampreys (Petromyzon marinus and Geotria australis) have been studied. One type of MR cell, which is found only in ammocoetes, occurs in groups on and between gill lamellae. Freeze-fracture replicas sh...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051003
更新日期：1998-02-01 00:00:00
abstract：:The true surface of rabbit spinal ganglion neurons has been made directly accessible to scanning electron-microscope observation after removal of both the connective tissue and satellite cells that normally cover it. The neuronal surface is characterized by a profusion of slender projections whose shapes have been det...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297502
更新日期：1990-04-01 00:00:00
abstract：:We have examined IgG Fc receptor (FcR) activity of human and rabbit arachnoid granulations and leptomeninges using antibody (IgG)-coated erythrocytes (EIgG), covalently crosslinked IgG dimers, trimers and oligomers, immune complexes, aggregated Fc fragments and a monoclonal anti-human neutrophil Fc receptor antibody, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216489
更新日期：1987-06-01 00:00:00
abstract：:We investigated the binding characteristics of two monoclonal antibodies, 4F3 and 3F8, which in the retina specifically stain Müller cells, both with protein blots and immunohistochemically in sections of various regions of the central nervous system of neonatal and adult cats and rats. Clear differences emerged betwe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050887
更新日期：1997-09-01 00:00:00
abstract：:Thyroid hormone (TH) plays an important role in regulating ovarian development. However, the mechanism involved remains unclear. Evidence suggests that glucose is essential for ovarian development, and its uptake is mediated by several glucose transporter proteins (Glut). We have investigated the effects of TH on Glut...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-016-2453-3
更新日期：2016-11-01 00:00:00
abstract：:Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0430-6
更新日期：2007-09-01 00:00:00
abstract：:A body of evidence points to the existence of stem cell stores in adult tissues, in addition to the well-known hematopoietic stem cells from bone marrow. Many reports describe the ability of these multipotent cells (developmentally non-compromised with their organs of origin) to give rise to many different cell types ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-006-0172-x
更新日期：2006-09-01 00:00:00
