Abstract：
:Although Hertwig's epithelial root sheath (HERS) performs an important function in the formation of the tooth root, the developmental mechanisms that control HERS growth and differentiation remain to be thoroughly elucidated. Bone morphogenetic protein 4 (BMP4), which is secreted by mesenchymal cells, acts on the dental epithelium as a regulator of cell differentiation during crown formation. In an effort to determine whether BMP4 specifically regulates the development of HERS in the dental epithelium, we assessed the localizations of BMP4, BMP receptor-IB (BMPR-IB), and BMPR-II during molar root formation in the mouse. HERS cells were shown to express BMPR-IB and BMPR-II. BMP4-positive cells were detected densely in the dental papillae around HERS, thereby suggesting that BMP4 participated in HERS formation. Beads soaked in BMP4, NOGGIN, or phosphate-buffered saline (PBS) were implanted into the pulp cavity under culture conditions, and the length of HERS was evaluated with regard to the proliferating cells. After 12 h, both groups exhibited a similar HERS developmental pattern, with the length and shape of HERS bearing a close resemblance to one another. However, after 48 h, the observed HERS elongation was significantly shorter in the BMP4-treated group. In addition, proliferative cell nuclear antigens were detectable only in the NOGGIN- and PBS-treated groups. These findings demonstrate that mesenchymally expressed BMP4 regulates HERS development by preventing elongation and maintaining cell proliferation. BMP4 may, therefore, prove useful as a root-formation regulatory agent in a variety of tissue-engineering applications.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Hosoya A,Kim JY,Cho SW,Jung HSdoi
10.1007/s00441-008-0655-zsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
503-9issue
3eissn
0302-766Xissn
1432-0878journal_volume
333pub_type
杂志文章abstract：:The existence of a neural crest cell migration pathway from occipital levels of the hindbrain into the heart was suspected in mammalian embryos because it had previously been identified in avian embryos and because the Di George anomaly, an association between craniofacial and cardiac malformations, is most easily exp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00338048
更新日期：1992-04-01 00:00:00
abstract：:The azygos vein of the rat can be divided into three regions: The proximal cardiac region, where the wall is composed of two and sometimes three layers of cardiac muscle and a thin discontinuous layer of smooth muscle cells. Vascular casts of this region demonstrate layers of capillaries closely following the orientat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221867
更新日期：1986-01-01 00:00:00
abstract：:Following castration, the nucleus of the dark cells of the subcortical portions of the collecting tubules in the rat kidney showed two types of morphological alterations: 1) Decomposition of the nuclear membrane in a circumscribed area. This phenomenon is independent of the sex of the animal and becomes more frequent ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220204
更新日期：1980-01-01 00:00:00
abstract：:From its inception, tissue engineering has had three tenets: cells, biomaterial scaffolds and signaling molecules. Among the triad, cells are the center piece, because cells are the building blocks of tissues. For decades, cell therapies have focused on the procurement, manipulation and delivery of healthy cells for t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1339-2
更新日期：2012-03-01 00:00:00
abstract：:Captopril [1-(D-3-mercapto-2-methyl-1-oxopropyl)-L-proline (S,S)] is now known to be an inhibitor of angiotensin-converting enzyme. In male mice, the juxtaglomerular cells were examined by electron microscopy 42 h after administration of captopril. Numerous dense granules appeared in the rough endoplasmic reticulum of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209980
更新日期：1981-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2018-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-1126-x
更新日期：2011-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222373
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1812-1
更新日期：2014-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220749
更新日期：1978-09-05 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-0129-5
更新日期：2006-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219875
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03376-6
更新日期：2021-01-12 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327761
更新日期：1990-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222295
更新日期：1988-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219233
更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221865
更新日期：1986-01-01 00:00:00
abstract：:The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03341-3
更新日期：2020-11-25 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219951
更新日期：1975-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00231994
更新日期：1975-05-27 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220534
更新日期：1977-10-21 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319432
更新日期：1994-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222332
更新日期：1985-01-01 00:00:00
abstract：:The published online version contains mistake in Title and in Acknowledgement section. Please find below for the needed corrections. ...
journal_title：Cell and tissue research
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00441-018-2865-3
更新日期：2018-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:In this immunocytochemical study we investigated the distribution of nervous structures in the lower lip of adult rats. The region is characterized by a rich cutaneous and mucosal sensory innervation originating from terminal branches of the trigeminal system. Lower lip innervation was investigated by detection of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051348
更新日期：1999-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050518
更新日期：1996-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-015-2190-z
更新日期：2015-09-01 00:00:00
abstract：:The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221498
更新日期：1982-01-01 00:00:00
abstract：:The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210015
更新日期：1981-01-01 00:00:00
