Abstract：
:The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a -RFamide C-terminus and are structurally similar to sulfakinin peptides; in contrast, DSK 0 contains -SFamide and is not structurally similar to sulfakinins. Antisera specificities were determined by the design of the antigens and confirmed by dot blot analysis and preincubation with peptides prior to their use in immunocytochemistry. The distribution of immunoreactivity suggests that all three DSK peptides are processed from the polypeptide precursor and expressed in many of the same cells. Expression was observed at all developmental stages with an increase in the level of staining and the number of immunoreactive cells as development progresses. Cells in the brain lobe, optic lobe, subesophageal ganglion, thoracic ganglia, and the eighth abdominal neuromere contain DSK-immunoreactive materials. Immunoreactive fibers project from some cells and extend into the brain and ventral ganglion with regions of extensive arborization. DSK 0 immunoreactivity provides initial evidence for the presence of a -SFamide peptide in neural tissue. The observed expression of DSK-immunoreactive materials throughout development in numerous cells of the central nervous system suggests that DSK peptides may serve as hormones, modulators, or transmitters involved in several functions.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Nichols R,Lim IAdoi
10.1007/s004410050518subject
Has Abstractpub_date
1996-01-01 00:00:00pages
107-16issue
1eissn
0302-766Xissn
1432-0878journal_volume
283pub_type
杂志文章abstract：:Connective tissue microfibrils were observed in tissues prepared with methods believed to minimize the loss of tissue components. The eyes of C57BL/6J mice were fixed with glutaraldehyde followed by either freeze substitution, or embedding in glycol methacrylate, a water-miscible embedding medium, after limited or no ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318486
更新日期：1995-02-01 00:00:00
abstract：:In the intestine of the filtering zooids of B. schlosseri three segments can be distinguished. In the intermediate segment the epithelium, which is encrusted by the ampullae of the pyloric gland, shows marked aspects of alteration. In the proximal and distal segments, ciliated mucous, vacuolated and endocrine-like cel...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219771
更新日期：1977-08-26 00:00:00
abstract：:Effects of prolactin on the secretion of milk proteins have been investigated by incubating mammary tissue fragments from lactating rabbits. Within 15 min of adding the hormone to the incubation medium, cell morphology is modified: the relative volume occupied by the Golgi region is greatly increased. When prolactin i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220616
更新日期：1978-02-14 00:00:00
abstract：:To investigate the role of RhoA on the intracellular membrane dynamics of lysosomes in rat hepatoma cells (MM1), we analyzed the localization of lysosomal aspartic proteinase cathepsin D by confocal immunofluorescence microscopy in the dominant active RhoA-transfected cells. Here we show that the transfection of the d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:The formaldehyde-induced fluorescence technique had shown 5-hydroxytryptamine-containing enteric neurons in the intestine of the teleost Platycephalus bassensis, but did not reveal such neurons in the intestine of Tetractenos glaber or Anguilla australis. Re-examination of these animals with 5-hydroxytryptamine immuno...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226505
更新日期：1988-01-01 00:00:00
abstract：:The expression of corticosteroids and regional expression 16 (CR16) and neural Wiscott-Aldrich syndrome protein (N-WASP) was studied in the testes of men with idiopathic azoospermia by (1) immunohistochemistry and Western blot to detect the expression of CR16 and N-WASP protein, (2) reverse transcription with the poly...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1159-9
更新日期：2011-06-01 00:00:00
abstract：:Hepatic peroxisomes in human embryos with a menstrual age of 6 and 7 weeks have been examined via catalase cytochemistry. In the younger sample, the organelles show no catalase activity, their matrix being pale and coarsely reticular. In the 7-week specimen, the peroxisome population consists of catalase-positive and ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00323574
更新日期：1993-04-01 00:00:00
abstract：:The kidney is an organ involved in cross talk with many human organs. The link between the immune system and the kidney has been studied in some detail, although data precisely elucidating their interaction are sparse, in particular with regard to the function of the kidney filter apparatus. Current research suggests ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-017-2635-7
更新日期：2017-07-01 00:00:00
abstract：:In the small intestine of the pig, neuromedin U (NMU)-immunoreactivity was mainly confined to the nerve plexus of the inner submucosal and mucosal regions. After colchicine treatment, a high number of immunoreactive nerve cell bodies was observed in the plexus submucosus internus (Meissner), whereas only a low number ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239453
更新日期：1989-11-01 00:00:00
abstract：:Previous neurohistological studies have been extended to include the structures contained solely or mainly within the junctional esophageal segment which may play an important role in the sphincter mechanism. The main findings were: 1) a progressive cranio-caudal thickening of the muscularis mucosae; 2) a conspicuous ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215998
更新日期：1976-05-26 00:00:00
abstract：:Noses are extremely sophisticated chemical detectors allowing animals to use scents to interpret and navigate their environments. Odor detection starts with the activation of odorant receptors (ORs), expressed in mature olfactory sensory neurons (OSNs) populating the olfactory mucosa. Different odorants, or different ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03327-1
更新日期：2021-01-06 00:00:00
abstract：:Mast cells (MCs) and airway nerves play an important role in allergic asthma. However, little is known about the MCs and their interaction with airway nerves during allergic airway inflammation. This study aims to investigate the distribution and proliferation of MC populations in different lung compartments, along wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2597-9
更新日期：2017-08-01 00:00:00
abstract：:The spermatogonial stem cell line in prepubertal and adult bovine testis was studied by electron microscopy and protein gene product 9.5 immunohistochemistry. Three successive spermatogonia precursor cell configurations were observed. Small basal stem cells were found to possess a spherical shape and nuclei with two t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00583394
更新日期：1995-08-01 00:00:00
abstract：:Dome epithelium (DE), the tissue covering lymphoid domes of gut-associated lymphoid tissues, was examined in both adult and neonatal rabbit appendix or sacculus rotundus to determine if dome epithelial cells matured earlier than epithelial cells covering adjacent villi. The localization of well-differentiated epitheli...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216473
更新日期：1987-06-01 00:00:00
abstract：:PU.1, which is a transcription factor, promotes the terminal differentiation of alveolar macrophages (AMs). Its expression is regulated by granulocyte/macrophage colony-stimulating factor (GM-CSF). In this study of AMs in newborn rats, we performed immunohistochemical staining, acid phosphatase staining, reverse trans...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0405-7
更新日期：2007-07-01 00:00:00
abstract：:The electric organs of embryonic Torpedo marmorata have been reacted with three cationic stains to evaluate the appearance and distribution of anionic sites. Ruthenium red, alcian blue and lysozyme were used at different pHs and found to react in a time-related manner to anionic components within the interelectrocyte ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214662
更新日期：1987-10-01 00:00:00
abstract：:Immunoreactivity for calcitonin gene-related peptide is demonstrated for the first time in neuroepithelial bodies in the lung of newborn cats after Bouin fixation and embedding in paraffin. The intense staining clearly identifies these bodies at the level of bronchioli and alveoli. Occasionally, single neuroepithelial...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215517
更新日期：1987-08-01 00:00:00
abstract：:Electron microscopy of the pineal receptor cells in light- and dark-adapted brook trout, Salvelinus fontinalis and the rainbow trout, Salmo gairdneri, revealed no significant differences in the tubular and filamentous elements of the inner segment, neck and supranuclear regions. However, changes in synaptic relations ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234177
更新日期：1980-01-01 00:00:00
abstract：:The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5) in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical locali...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217869
更新日期：1984-01-01 00:00:00
abstract：:A tumor model system of clones of myeloproliferative sarcoma virus (MPV)-transformed rat fibroblasts (NRK) with different growth properties and metastatic potential was studied. The relationship between metastatic behavior and composition of carbohydrate-binding proteins (lectins) was analyzed by affinity chromatograp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215191
更新日期：1986-01-01 00:00:00
abstract：:The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219936
更新日期：1975-12-18 00:00:00
abstract：:The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218017
更新日期：1985-01-01 00:00:00
abstract：:In Rana temporaria tadpoles, fluorescent fibres appear in the prospective eminentia mediana, the pars intermedia and the pars distalis at Gosner's stage 25. During prometamorphosis the amount of fluorescent material increases around the developing primary capillary plexus in the eminentia mediana. In the pars intermed...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220236
更新日期：1976-09-01 00:00:00
abstract：:The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03197-7
更新日期：2020-11-01 00:00:00
abstract：:DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness cau...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-1062-8
更新日期：2005-04-01 00:00:00
abstract：:Seven isoforms of tachykinin-related peptides (TRPs) have been isolated from the brain of the cockroach Leucophaea maderae. These peptides (LemTRP-1, 2, and 5-9) share the C-terminal sequence GFX(1)GX(2)Ramide (where X(1) and X(2) are variable residues). In order to determine the neuronal distribution of several of th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0538-7
更新日期：2002-05-01 00:00:00
abstract：:Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051013
更新日期：1998-03-01 00:00:00
abstract：:Intracellular sporozoan parasites invade the host cell through the invagination of the plasma membrane of the host and a vacuole is formed which accommodates the entering parasite. The vacuole may disappear and the invaginated membrane of the host then becomes closely apposed to that of the parasite's own membrane. As...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219605
更新日期：1976-06-28 00:00:00
abstract：:We have previously shown that mice deficient in the gene for the myelin-associated glycoprotein (MAG) develop normal myelin in the peripheral nerves, but show axon and myelin degeneration at eight months of age, suggesting that MAG is involved in the maintenance of axon-Schwann cell integrity. The search for molecules...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050727
更新日期：1997-01-01 00:00:00
abstract：:Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a leng...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220391
更新日期：1978-11-20 00:00:00
