Abstract：
:The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the formation of the acrosome during spermatogenesis. Male ICR mice are injected with GSK923295 for long-term inhibition of CENP-E. Chemical inhibition and siRNA-mediated knockdown of CENP-E are carried out in the GC-2 spd cells. The morphology of the acrosomes is determined by the HE staining, immunofluorescence, and transmission electron microscopy. We have identified CENP-E is a key factor in the formation and structural maintenance of the acrosome during acrosome biogenesis. Long-term inhibition of CENP-E by GSK923295 results in the asymmetric acrosome and the dispersed acrosome. CENP-E depletion leads to the malformation of the Golgi complex and abnormal targeting of the PICK1- and PIST-positive Golgi-associated vesicles. Our findings uncover an essential role of CENP-E in membrane trafficking and structural organization of the acrosome in the spermatids during spermatogenesis. Our results shed light on the molecular mechanisms involved in vesicle trafficking and architecture maintenance of the acrosome.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
She ZY,Yu KW,Wei YL,Zhong N,Lin Ydoi
10.1007/s00441-020-03341-3subject
Has Abstractpub_date
2020-11-25 00:00:00eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-020-03341-3pub_type
杂志文章abstract：:Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222069
更新日期：1975-01-01 00:00:00
abstract：:The treatment of isolated protoplasmic strands of Physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. T...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218017
更新日期：1985-01-01 00:00:00
abstract：:In the small intestine of the pig, neuromedin U (NMU)-immunoreactivity was mainly confined to the nerve plexus of the inner submucosal and mucosal regions. After colchicine treatment, a high number of immunoreactive nerve cell bodies was observed in the plexus submucosus internus (Meissner), whereas only a low number ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239453
更新日期：1989-11-01 00:00:00
abstract：:The postnatal maturation of regions of the epididymis and intragonadal segment of the deferens duct was studied in the rat by light- and transmission electron microscopy. Maturation of the genital duct starts in the distal cauda epididymidis and ductus deferens after one week of life, and one week later, in the more c...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215508
更新日期：1987-08-01 00:00:00
abstract：:Radiofrequency lesions were made in the lateral geniculate nuclei of six squirrel monkeys. The resulting degenerating terminals and their postsynaptic structures in layer IV of area 17 were quantitatively categorized on photomontages covering large areas of neuropil. Two to five days after the lesion, numerous axon te...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234741
更新日期：1979-02-28 00:00:00
abstract：:In the atherosclerotic plaque, smooth muscle cells (SMC) acquire an inflammatory phenotype. Resistin and fractalkine (CX3CL1) are found in human atheroma and not in normal arteries. CX3CL1 and CX3CR1 are predominately associated with SMC. We have questioned whether resistin has a role in the expression of CX3CL1 and C...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1510-9
更新日期：2013-01-01 00:00:00
abstract：:alpha-Smooth muscle (alpha SM) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha SM-1. Immunoreactivity to this antibody has been shown to be localized in the NH2-terminal sequence Ac-EEED (Chaponnier et al. 1994). Among terrestrial ectothermic vertebrates, two amphibian ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00417867
更新日期：1995-09-01 00:00:00
abstract：:Although cartilage is considered to be devoid of innervation, axons occur in the perichondrium and during development in cartilage canals, thereby having a relatively close spatial relationship to chondroblasts and chondrocytes. The present study locates the source of the sensory innervation of the femoral cartilagino...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900142
更新日期：2000-02-01 00:00:00
abstract：:Acute necrotizing pancreatitis (ANP) is a common gastrointestinal cause of emergency admissions in dogs and humans and can lead to a systemic inflammatory response syndrome resulting in multiple organ dysfunction syndrome. Among the various complications associated with ANP, acute lung injury (ALI) or acute respirator...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03211-y
更新日期：2020-05-01 00:00:00
abstract：:Luteinizing hormones-releasing hormone (LHRH), vasopressin, and corticotropin systems were examined by immunocytochemical methods in male rats 2 to 20 days after deafferentation of the basal hypothalamus. Axonal degeneration of the vasopressin system (whose perikarya lie rostral to the island) and the corticotropin sy...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210157
更新日期：1981-01-01 00:00:00
abstract：:The electron-dense tracers ferritin, and iron-dextran, and the protein horseradish peroxidase, have been used to investigate the ultrastructural basis of permeability in the upper and lower segments of the Malpighian tubules of Glomeris marginata. All these materials were able to cross the basal lamina and enter the t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219837
更新日期：1977-09-05 00:00:00
abstract：:The LIM homeodomain transcription factor Lmx1a shows a dynamic expression in the developing mouse ear that stabilizes in the non-sensory epithelium. Previous work showed that Lmx1a functional null mutants have an additional sensory hair cell patch in the posterior wall of a cochlear duct and have a mix of vestibular a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03163-y
更新日期：2020-06-01 00:00:00
abstract：:Complex intracellular inclusion bodies of the Biondi type were observed in the choroidal epithelium (choroid plexus of the lateral ventricle) of a 43-year-old male chimpanzee. The specific components of these inclusions are bundles of filaments 8-15 nm in diameter, which are associated with lipid droplets and a wide v...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217876
更新日期：1984-01-01 00:00:00
abstract：:The three segments (S1, S2, S3) of the proximal tubule of the rat kidney were investigated, with special reference to lysosomes, after castration, estradiol application, and at the end of pregnancy. Especially in S1 and S2 castration induces an increase of cellular autophagy. The nuclei become smaller; endoplasmic ret...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00212331
更新日期：1978-09-26 00:00:00
abstract：:Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00361712
更新日期：1976-08-10 00:00:00
abstract：:Adult white-footed mice, Peromyscus leucopus, were kept either in long photoperiod (LP; LD 16:8) or in short photoperiod (SP; LD 8:16) for six weeks. Ovaries were serially sectioned and examined histologically. Preantral follicles were observed to release their oocyte into the ovarian stroma. This phenomenon is termed...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218373
更新日期：1982-01-01 00:00:00
abstract：:Rainbow trout (Oncorhynchus mykiss) were exposed to ion-poor (soft) water to test the hypothesis that naturally induced proliferation of branchial chloride cells causes a thickening of the blood-to-water diffusion barrier. This was achieved by using a combination of scanning and transmission electron-microscopic techn...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050622
更新日期：1996-07-01 00:00:00
abstract：:A total of 20 Golgi-impregnated cone horizontal cells of Nannacara anomala (Cichlidae) were studied in alternating semi- and ultrathin sections in order to examine their connections with the overlying square mosaic of equal double and central single cones. Cone horizontal cells exhibiting three types of processes: (a)...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220825
更新日期：1976-11-24 00:00:00
abstract：:Intracerebroventricular (ICV) injection of melanin-concentrating hormone (MCH) influences feeding behavior in the goldfish and exerts an anorexigenic action in goldfish brain, unlike its orexigenic action in mammals. Despite a growing body of knowledge concerning MCH function in mammals, the role of MCH in appetite ha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0347-5
更新日期：2007-05-01 00:00:00
abstract：:Chemoreception in marine invertebrates mediates a variety of ecologically important behaviors including defense, reproduction, larval settlement and recruitment, and feeding. The sensory pathways that regulate deposit-feeding activity by polychaetes living in sedimentary habitats are of particular interest because suc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0660-2
更新日期：2008-09-01 00:00:00
abstract：:The desert locust Schistocerca gregaria is a major agricultural pest in North Africa and the Middle East. As such, it has been intensely studied, in particular with respect to population dynamics, sensory processing, feeding behavior flight and locomotor control, migratory behavior, and its neuroendocrine system. Bein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2844-8
更新日期：2018-10-01 00:00:00
abstract：:To investigate the role of RhoA on the intracellular membrane dynamics of lysosomes in rat hepatoma cells (MM1), we analyzed the localization of lysosomal aspartic proteinase cathepsin D by confocal immunofluorescence microscopy in the dominant active RhoA-transfected cells. Here we show that the transfection of the d...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000243
更新日期：2000-09-01 00:00:00
abstract：:Injection of wheat-germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the superior cervical ganglion (SCG) of the rat results in accumulation of WGA-HRP in sympathetic postganglionic neurons in the contralateral SCG. The sympathetic pathways involved and the mechanism underlying the labeling were investig...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327745
更新日期：1990-10-01 00:00:00
abstract：:The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various ma...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0666-9
更新日期：2008-10-01 00:00:00
abstract：:Numerous macrophages were found aggregated in the lamina propria at the tips of villi in the small intestine of guinea pigs. These macrophages extended their pseudopodia into the epithelial lining and internalized fragments of effete enterocytes in their phagosomes. The epithelium of the villus tips was found to be in...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF02913723
更新日期：1993-03-01 00:00:00
abstract：:Measurements of 3H-thymidine incorporation, quantitative autoradiography and morphometry were used to evaluate cell behavior during the recovery of mandibular condylar cartilage cultures following short-term exposure to a corticosteroid hormone in vitro. Apical segments of mandibular condyles of newborn mice were init...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297231
更新日期：1990-05-01 00:00:00
abstract：:The protein synthesis inhibitor cycloheximide (Chx) suppresses prolactin-induced beta-casein gene expression in the mammary epithelial cell line COMMA-D. As the mechanism underlying this effect is unclear, the effects of protein synthesis inhibitors on interactions of transcription factors with the beta-casein promote...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0672-2
更新日期：2003-02-01 00:00:00
abstract：:Enterochromaffin (EC) cells regulate gut motility and secretion in response to luminal stimuli, via the release of serotonin (5-HT). Inflammatory bowel disease and other gastrointestinal disorders are associated with increased numbers of EC cells and 5-HT availability. Our aim was to determine whether proliferation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0430-6
更新日期：2007-09-01 00:00:00
abstract：:Isotocinergic (IT) neurons show physiological and morphological characteristcs that are similar to those of the other preoptic neuroendocrine cells in the goldfish. Preoptic IT cells show resting membrane potentials of 20-55 mV, action potentials of up to 100 mV, and physiological evidence of axonal branching. Dye-mar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00239217
更新日期：1979-10-02 00:00:00
abstract：:The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219054
更新日期：1977-03-09 00:00:00
