Uptake of horseradish peroxidase by bone cells during endochondral bone development.

Abstract:

:To investigate the mechanisms whereby bone cells absorb organic bone-matrix components during endochondral bone development, rat humeri were examined, employing horseradish peroxidase as a soluble protein tracer. Intravenously-injected peroxidase filled the osteoid layer and penetrated into the osteocyte lacunae and canaliculi, but did not enter the mineralized bone matrix. Whereas osteocytes rarely took up exogenous peroxidase, osteoblasts and osteoclasts actively endocytosed peroxidase in pinocytotic coated vesicles, tubular structures, and vacuoles. They also formed endocytotic vacuoles containing peroxidase in the Golgi area. The Golgi apparatus and dense bodies of these bone cells were, however, free of reaction products. Osteoclast ruffled borders were responsible for peroxidase absorption. In the osteoblast, osteocyte and osteoclast, endogenous peroxidatic reaction was detected only in mitochondria and not in other membrane-bounded vesicles and bodies. These results strongly suggest that both osteoblasts and osteoclasts participate in the resorption of bone-matrix organic components during bone remodelling.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Sasaki T,Yamaguchi A,Higashi S,Yoshiki S

doi

10.1007/BF00219233

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

547-53

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

239

pub_type

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