Sex differences in pituitary LH storage and release in LHRH-stimulated pubertal rats. A correlative immunohistochemical and radioimmunoassay study.

Abstract:

:This study investigates the relationship between pituitary LHRH responsiveness and the depletion of LH in pubertal rats. The anterior pituitaries of 7-week-old rats of both sexes were stimulated for a maximum of 24 h with either a continuous, or pulsatile exposure to LHRH in vitro. Immunohistochemical examination revealed that most LH-cells in females became depleted of immunoreactive material, regardless of the mode of LHRH administration. In contrast, the majority of LH-cells in the male gland retained a strong immunostaining intensity. Radioimmunoassay showed that the initial pituitary LH content was significantly lower in the female rats (P less than 0.001), but, even so, they released a higher percentage of stored LH in response to LHRH stimulation in vitro. A similar result was also obtained after a single injection of LHRH in vivo. Thus, the lower LH content and higher LHRH responsiveness of the female pituitary explain why LHRH treatment induced a pronounced LH depletion in this sex. These results are discussed in relation to available data on heightened LH secretion in maturing female rats.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Watanabe YG

doi

10.1007/BF00218057

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

509-15

issue

3

eissn

0302-766X

issn

1432-0878

journal_volume

243

pub_type

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