Basal lamina and other extracellular matrix produced by bovine granulosa cells in anchorage-independent culture.


:Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30-100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antral follicles. Thus, granulosa cells in anchorage-independent cultures have a follicular cell morphology and secrete two distinct extracellular matrices, one similar to the follicular basal lamina.


Cell Tissue Res


Cell and tissue research


Rodgers HF,Lavranos TC,Vella CA,Rodgers RJ




Has Abstract


1995-12-01 00:00:00












  • Effect of pimozide on the cytology of the eel pituitary. II. MSH-secreting cells.

    abstract::Pimozide, a specific blocker of dopaminergic receptors, was injected for 4 or 9 days in freshwater (FW) eels or eels acclimated to sea water (SW) for 10 or 30 days. The daily dose was 100 or 200 microgram/100 g. Melanophore index values increase in FW and in 1 month-SW injected eels. All the treated fish react by a to...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Olivereau M

    更新日期:1978-05-29 00:00:00

  • Ultrastructure of the nongranulated cells and morphology of the extracellular spaces in the pars distalis of adult and pouch-young tammar wallabies (Macropus eugenii).

    abstract::Differentiated nongranulated (folliculo-stellate) cells were observed in the centre and periphery of the cords of cells in the pars distalis of the adult tammar wallaby (Macropus eugenii). The nongranulated cells formed follicles containing a small lumen: the cell apices were joined by junctional complexes including z...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Leatherland JF,Renfree MB

    更新日期:1982-01-01 00:00:00

  • Scanning electron microscopic study of the freeze-fractured pineal body of the rat.

    abstract::The three-dimensional ultrastructure of the pineal body of the rat is described on the basis of freeze-fractured preparations. The pineal capsule consists of irregular cells with very flat and perforated processes. Through these openings, extremely branched canaliculi, extending to almost every pineal cell, communicat...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Krstić RV

    更新日期:1979-09-02 00:00:00

  • Cytomorphological study on human submandibular gland following treatment with secretagogue drugs.

    abstract::Using specimens of human submandibular glands, we have investigated in vitro the morphological modifications induced by clozapine, a dibenzodiazepine derivative that is used in psychotic patients and that provokes hypersalivation, a side-effect of therapy. The effects of the drug, used alone or in combination with car...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Testa Riva F,Puxeddu R,Loy F,Conti G,Riva A

    更新日期:2006-05-01 00:00:00

  • Challenges in the development of glaucoma neuroprotection therapy.

    abstract::Glaucoma, a disease of the optic nerve and retina, causes blindness in millions of people worldwide. Currently available therapies for this disease only attempt to reduce intraocular pressure, the major risk factor, without addressing the associated optic neuropathy and retinopathy. Development of glaucoma neuroprotec...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Liu Y,Pang IH

    更新日期:2013-08-01 00:00:00

  • Induction by progesterone of a sexual dimorphism of estrogen uptake by anterior pituitary cells in situ.

    abstract::The effects of progesterone pretreatment on in vivo 3H-estrogen uptake by five anterior pituitary cell types was analyzed by means of a quantitative autoradiographic-immunocytochemical technique. Male and female rats castrated for 14 days show nuclear concentration of label in all five cell types one h after injection...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Keefer DA

    更新日期:1981-01-01 00:00:00

  • A new type of gap junction in the phylum Brachiopoda.

    abstract::Gap junctions in the phylum Brachiopoda are shown to be of the P type as has been previously reported in invertebrates only in molluscs and possibly bryozoans. However, the mollusc gap junctions appear identical to vertebrae gap junctions in the fracture face on which particles remain, in particle spacing and in parti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Flower NE,Green CR

    更新日期:1982-01-01 00:00:00

  • Biochemical and histological analysis of two Müller cell antibodies in developing and adult cat and rat central nervous system.

    abstract::We investigated the binding characteristics of two monoclonal antibodies, 4F3 and 3F8, which in the retina specifically stain Müller cells, both with protein blots and immunohistochemically in sections of various regions of the central nervous system of neonatal and adult cats and rats. Clear differences emerged betwe...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Distler C,Bronzel M,Paas I,Wahle P

    更新日期:1997-09-01 00:00:00

  • Studies on the surface coat of Paramecium aurelia. II. Relationship to the immobilization antigen.

    abstract::Correlations between the presence of surface coat and immobilization antigen of Paramecium tetraurelia were studied. Supravital, partial removal of the surface coat resulted in accelerated response of monobacterially and axenically grown cells to the homologous antiserum. Ciliates pretreated with trypsin or pronase (0...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Wyroba E

    更新日期:1977-07-11 00:00:00

  • The distribution of GABA and glycine immunostaining in the cochlear nucleus of the mustached bat (Pteronotus parnellii).

    abstract::The distribution of neuronal elements immunoreactive for gamma-aminobutyric acid (GABA) and glycine in the cochlear nucleus of the mustached bat Pteronotus parnellii has been studied by means of the postembedding technique on serial semithin sections. Our goal has been to identify similarities and differences in the o...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kemmer M,Vater M

    更新日期:1997-02-01 00:00:00

  • Location of PHM/VIP mRNA in human gastrointestinal tract detected by in situ hybridization.

    abstract::The expression of the gene for vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) in the human gastrointestinal tract was studied by in situ hybridization and Northern blotting for PHM/VIP mRNA and immunocytochemistry using specific antisera against the bioactive peptides PHM and VIP. In th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bredkjaer HE,Wulff BS,Emson PC,Fahrenkrug J

    更新日期:1994-05-01 00:00:00

  • Ventricular remodeling of single-chambered myh6-/- adult zebrafish hearts occurs via a hyperplastic response and is accompanied by elastin deposition in the atrium.

    abstract::Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sarantis P,Gaitanaki C,Beis D

    更新日期:2019-11-01 00:00:00

  • Surface fine structure of the globiferous pedicellariae of the regular echinoid, Psammechinus miliaris Gmelin.

    abstract::The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Oldfield SC

    更新日期:1975-10-13 00:00:00

  • Tooth development in vitro in two teleost fish, the cichlid Hemichromis bimaculatus and the cyprinid Danio rerio.

    abstract::A technique for organotypic in vitro culture with serum-free medium was tested for its appropriateness to mimic normal odontogenesis in the cichlid fish Hemichromis bimaculatus and the zebrafish Danio rerio. Serial semithin sections were observed by light microscopy to collect data on tooth patterning and transmission...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Van der Heyden C,Allizard F,Sire JY,Huysseune A

    更新日期:2005-09-01 00:00:00

  • Reduced level of interphotoreceptor retinoid-binding protein (IRBP), a possible cause for retinal degeneration in the Abyssinian cat.

    abstract::Retinae of Abyssinian cats homozygous for a retinal degeneration gene, and normal controls, have been investigated using antibodies directed against opsin, transducin alpha (TD-alpha), S-antigen (48K protein), interphotoreceptor retinoid-binding protein (IRBP), and cone outer segments. IRBP-immunoreactivity (IR) is mu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Narfström K,Nilsson SE,Wiggert B,Lee L,Chader GJ,van Veen T

    更新日期:1989-09-01 00:00:00

  • Interendothelial junctions in kidney vessels.

    abstract::The interendothelial junctions of all segments of the renal vasculature have been studied in eight species using the freeze-fracture technique. Three types of junctions have been found. Combinations of tight and gap junction elements are characteristic for interlobular arteries and proximal afferent arterioles. Contin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Mink D,Schiller A,Kriz W,Taugner R

    更新日期:1984-01-01 00:00:00

  • Predicting response to cancer chemotherapy: the role of p53.

    abstract::Loss of wild-type p53 activity is thought to be a major predictor of failure to respond to radiotherapy and chemotherapy in various human cancers. This assumption is largely based on some cell-death studies in p53-knockout mice and on correlations of p53 status assessed by immunochemistry or single-strand conformation...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Weller M

    更新日期:1998-06-01 00:00:00

  • Thrombin regulates nerve growth factor secretion from vascular, but not bladder smooth muscle cells.

    abstract::The production of nerve growth factor (NGF) in peripheral organs may play a role in the pathophysiology of hypertension and in obstructive disorders of the bladder outlet. We have been examining the cellular processes of NGF delivery and secretion in smooth muscle. NGF secretion from vascular smooth muscle cells (VSMC...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sherer TB,Spitsbergen JM,Steers WD,Tuttle JB

    更新日期:1997-07-01 00:00:00

  • Skin-derived multipotent stromal cells--an archrival for mesenchymal stem cells.

    abstract::Progenitor stem cells have been identified, isolated and characterized in numerous tissues and organs. However, their therapeutic potential and the use of these stem cells remain elusive except for a few progenitor cells from bone marrow, umbilical cord blood, eyes and dental pulp. The use of bone marrow-derived hemat...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Vishnubalaji R,Al-Nbaheen M,Kadalmani B,Aldahmash A,Ramesh T

    更新日期:2012-10-01 00:00:00

  • Effect of hydrocortisone, reserpine, propranolol and phentolamine on in vivo uptake of exogenous amines by adrenal chromaffin cells.

    abstract::An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone ahd no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kent C,Monkhouse WS,Coupland RE

    更新日期:1981-01-01 00:00:00

  • Shaping oral cell plasticity to osteogenic differentiation by human mesenchymal stem cell coculture.

    abstract::In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Proksch S,Steinberg T,Vach K,Hellwig E,Tomakidi P

    更新日期:2014-04-01 00:00:00

  • Early effects of prolactin on lactating rabbit mammary gland. Ultrastructural changes and stimulation of casein secretion.

    abstract::Effects of prolactin on the secretion of milk proteins have been investigated by incubating mammary tissue fragments from lactating rabbits. Within 15 min of adding the hormone to the incubation medium, cell morphology is modified: the relative volume occupied by the Golgi region is greatly increased. When prolactin i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ollivier-Bousquet M

    更新日期:1978-02-14 00:00:00

  • Daily fluctuations of nucleoli in neurosecretory cells of the rat supraoptic nucleus. An ultrastructural and stereological study.

    abstract::Daily fluctuations of nucleoli and nucleolar fibrillar centres in neurosecretory cells from the supraoptic nucleus (SON) were investigated in rats artificially synchronized for 3 weeks to a set 12 h light/12 h dark cycle with free access to food and water. Group of 3 animals were sacrificed by intracardiac perfusion e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bessone R,Seïte R

    更新日期:1985-01-01 00:00:00

  • Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome.

    abstract::The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: She ZY,Yu KW,Wei YL,Zhong N,Lin Y

    更新日期:2020-11-25 00:00:00

  • FoxO3a suppression and VPS34 activity are essential to anti-atrophic effects of leucine in skeletal muscle.

    abstract::Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were im...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Baptista IL,Silvestre JG,Silva WJ,Labeit S,Moriscot AS

    更新日期:2017-08-01 00:00:00

  • Recognition of sodium- and potassium-dependent adenosine triphosphatase in organs of the mouse by means of a monoclonal antibody.

    abstract::The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C. The antigen, purified from mouse brain by means of affinity chrom...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Gorvel JP,Liabeuf A,Massey D,Liot D,Goridis C,Maroux S

    更新日期:1983-01-01 00:00:00

  • Autonomic innervation of the ocular choroid membrane in the chicken: a fluorescence-histochemical and electron-microscopic study.

    abstract::The distribution pattern of adrenergic fibres innervating the ocular choroid membrane of the chicken was studied by means of fluorescence and electron microscopy. In addition, the origin of these fibres was investigated after superior cervical ganglionectomy. Adrenergic axons reach the choroid, partly forming the peri...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Guglielmone R,Cantino D

    更新日期:1982-01-01 00:00:00

  • Neurohormones as putative circadian clock output signals in the central nervous system of two cricket species.

    abstract::Antisera to the neuropeptides corazonin (Crz) and crustacean cardioactive peptide (CCAP) and to the diapause hormone (DH) react with small sets of neurones in the cephalic ganglia of the crickets Dianemobius nigrofasciatus and Allonemobius allardi. The distribution of their immunoreactivities is similar in the two spe...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sehadová H,Shao QM,Sehnal F,Takeda M

    更新日期:2007-04-01 00:00:00

  • Hepatic cellular distribution and degradation of iron oxide nanoparticles following single intravenous injection in rats: implications for magnetic resonance imaging.

    abstract::The purpose of this study was to determine the cellular distribution and degradation in rat liver following intravenous injection of superparamagnetic iron oxide nanoparticles used for magnetic resonance imaging (NC100150 Injection). Relaxometric and spectrophotometric methods were used to determine the concentration ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Briley-Saebo K,Bjørnerud A,Grant D,Ahlstrom H,Berg T,Kindberg GM

    更新日期:2004-06-01 00:00:00

  • Photoreceptor membrane breakdown in the spider Dinopis: localisation of acid phosphatases.

    abstract::The ultrastructural localisation of acid phosphatases (AcPhs) during the normal daily breakdown of rhabdomere membrane in Dinopis has been examined using beta-glycerophosphate and p-nitrophenyl phosphate as substrates. Results are related to the classification of organelles in the receptors given by Blest, Powell and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blest AD,Price GD,Maples J

    更新日期:1979-07-17 00:00:00