Basal lamina and other extracellular matrix produced by bovine granulosa cells in anchorage-independent culture.


:Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30-100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antral follicles. Thus, granulosa cells in anchorage-independent cultures have a follicular cell morphology and secrete two distinct extracellular matrices, one similar to the follicular basal lamina.


Cell Tissue Res


Cell and tissue research


Rodgers HF,Lavranos TC,Vella CA,Rodgers RJ




Has Abstract


1995-12-01 00:00:00












  • Immune-complex-trapping cells in the spleen of the oriental fire-bellied toad, Bombina orientalis.

    abstract::The spleen of the oriental fire-bellied toad, Bombina orientalis, consists of well-developed white pulp, separated from the lymphocytic marginal zone by the connective tissue boundary layer. Injection of peroxidase-conjugated rabbit anti-peroxidase revealed that these immune complexes were localized on the surface of ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dulak J,Bauer J,Döpp EA,Van Rooijen N

    更新日期:1993-01-01 00:00:00

  • Ultrastructural distribution of endogenous immunoglobulin-G in human term amniochorion.

    abstract::Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bright NA,Ockleford CD

    更新日期:1995-08-01 00:00:00

  • Ultracytochemical evidence for a proton-pump adenosine triphosphatase in chick osteoclasts.

    abstract::The distribution of Mg++-ATPase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline pH cytochemical methods at the electron-microscopic level. Reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. Levamisole, ouabain, and va...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Akisaka T,Gay CV

    更新日期:1986-01-01 00:00:00

  • Moulting hormone, juvenile hormone and the ultrastructure of the fat body of adult Sarcophaga bullata (Diptera).

    abstract::In the ovoviviparous fly, Sarcophaga bullata, vitellogenesis in cyclic; a process reflected in ultrastructural changes in the fat body cells and oenocytes. At eclosion the larval fat body has not yet completely disappeared. During vitellogenesis the fat body cells are specialized for intensive protein synthesis showin...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Stoppie P,Briers T,Huybrechts R,De Loof A

    更新日期:1981-01-01 00:00:00

  • Relationship of meiotic prophase and ornithine decarboxylase in the neonatal rabbit ovary.

    abstract::Ornithine decarboxylase (ODC) activity was measured in homogenates of neonatal rabbit ovaries from the day of birth to day 22, an interval that corresponds to the progressing stages of the meiotic prophase from oogonia to diplotene and follicle formation. The ODC-activity was high on days 1 and 3 when most germ cells ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: YoungLai EV,Byskov AG

    更新日期:1983-01-01 00:00:00

  • Actin in cellular components of the basement membrane of the compound eye of a blowfly.

    abstract::The so-called 'basement membrane' of arthropod compound eyes is known to be of heterogeneous origin (Odselius and Eloffson 1981). A major contribution in Diptera with open rhabdoms is provided by a pigmented component which lies at the basal end of the extracellular space of each ommatidium and fills it, the glial plu...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Blest AD,De Couet HG

    更新日期:1983-01-01 00:00:00

  • Light and electron microscopic studies on the excretory system of Macrobiotus richtersi Murray, 1911 (Eutardigrada).

    abstract::The excretory system of Macrobiotus richtersi consists of one dorsal and two lateral components and shows a high degree of structural complexity. In each of these a tricellular external lobe and a column can be distinguished, the two parts being connected distally. The surface of the lobe cells is increased by deep ba...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Weglarska B

    更新日期:1980-01-01 00:00:00

  • Immunologic localization of alpha- and beta-endorphins and beta-lipotropin in corticotropic cells of the normal and anencephalic fetal pituitaries.

    abstract::The use of antibodies to alpha-(17--39) ACTH, beta-LPH, and alpha- and beta-endorphins has enabled the detection of immunoreactive cells as early as the eight week of fetal life. Immunoreactive cells are located in the anterior and intermediate lobes of the pituitary gland in normal human fetuses. The same cells react...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bégeot M,Dubois MP,Dubois PM

    更新日期:1978-10-30 00:00:00

  • Anatomical organization of the cerebrum of the desert locust Schistocerca gregaria.

    abstract::The desert locust Schistocerca gregaria is a major agricultural pest in North Africa and the Middle East. As such, it has been intensely studied, in particular with respect to population dynamics, sensory processing, feeding behavior flight and locomotor control, migratory behavior, and its neuroendocrine system. Bein...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: von Hadeln J,Althaus V,Häger L,Homberg U

    更新日期:2018-10-01 00:00:00

  • Enkephalin-immunoreactive cells in the mesencephalic tegmentum project to the optic tectum of the teleosts Salmo gairdneri and Salmo salar.

    abstract::Immunocytochemistry using antibodies against Met-enkephalin and Leu-enkephalin has demonstrated a group of large enkephalin-immunoreactive neurons in the nucleus of the rostral mesencephalic tegmentum (mRMT) of two teleost fish. Salmo gairdneri and Salmo salar. Injections of cobalt-lysine in the medial optic tectum re...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Vecino E,Ekström P,Sharma SC

    更新日期:1991-04-01 00:00:00

  • Immuno-cytochemical demonstration, in the external region of the amphibian median eminence, of separate vasotocinergic and mesotocinergic nerve fibres.

    abstract::By means of single and double immuno-enzyme cytochemical staining techniques, it was shown that the external region of the amphibian median eminence contains separate vasotocinergic and mesotocinergic nerve fibres. Moreover, it was demonstrated that the vasotocinergic fibres also contain neurophysin. In animals in whi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dierickx K,Vandesande F

    更新日期:1977-02-02 00:00:00

  • Expression of lynx1 in developing lung and its modulation by prenatal nicotine exposure.

    abstract::The expression of nicotinic acetylcholine receptors (nAChR) in fetal lung suggests maternal smoking during pregnancy effects newborn lung structure and function by the direct interaction of nicotine with nAChR in the developing lung. The recent identification of the lynx1 nAChR modulator protein in nicotinic neurons i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sekhon HS,Song P,Jia Y,Lindstrom J,Spindel ER

    更新日期:2005-05-01 00:00:00

  • Intraepithelial inclusions resembling human biondi bodies in the choroid plexus of an aged chimpanzee.

    abstract::Complex intracellular inclusion bodies of the Biondi type were observed in the choroidal epithelium (choroid plexus of the lateral ventricle) of a 43-year-old male chimpanzee. The specific components of these inclusions are bundles of filaments 8-15 nm in diameter, which are associated with lipid droplets and a wide v...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Oksche A,Liesner R,Tigges J,Tigges M

    更新日期:1984-01-01 00:00:00

  • Quantitative analysis of denatured collagen by collagenase digestion and subsequent MALDI-TOF mass spectrometry.

    abstract::Collagens are the most abundant proteins in vertebrate tissues and constitute significant moieties of the extracellular matrix (ECM). The determination of the collagen content is of relevance not only in the field of native tissue research, but also regarding the quality assessment of bioengineered tissues. Here, we d...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Nimptsch A,Schibur S,Ihling C,Sinz A,Riemer T,Huster D,Schiller J

    更新日期:2011-03-01 00:00:00

  • Morphology and composition of the midgut bacterial community of Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae).

    abstract::The burrower bug Scaptocoris castanea is an important soybean and pasture pest in Brazil, with an underground habit feeding directly on the sap of the roots. Underground habit hinders control and knowledge of the biology and physiology of this pest. This study describes the anatomy, histology, ultrastructure and symbi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Cossolin JFS,Lopes DRG,Martínez LC,Santos HCP,Fiaz M,Pereira MJB,Vivan LM,Mantovani HC,Serrão JE

    更新日期:2020-11-01 00:00:00

  • Distribution of the extended family of protein kinase C isoenzymes in fetal organs of mice: an immunohistochemical study.

    abstract::Using isoenzyme-specific antisera, we have studied the distribution of protein kinase C isoforms in fetal mouse organs at the developmental age of 17 days. Two different sets of antibodies, produced by different manufacturers, were employed in this study. The specificity of the antisera was tested by immunoblotting ex...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bareggi R,Grill V,Zweyer M,Narducci P,Martelli AM

    更新日期:1995-06-01 00:00:00

  • Spatial and temporal regulation of glycosylation during Drosophila eye development.

    abstract::Glycosylation plays an essential role during development, in processes such as morphogen distribution, cell-to-cell communication, and extracellular matrix formation. Glycosylation is regulated during development in both a spatial and temporal manner. This study presents a detailed description of glycan distribution f...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Yano H,Yamamoto-Hino M,Goto S

    更新日期:2009-04-01 00:00:00

  • Tormogen cell and receptor-lymph space in insect olfactory sensilla. Fine structure and histochemical properties in Calliphora.

    abstract::(1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell (one of the three accessory cells) forms microvilli coated internally with particles. (3) In ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Gnatzy W,Weber KM

    更新日期:1978-06-08 00:00:00

  • Shaping oral cell plasticity to osteogenic differentiation by human mesenchymal stem cell coculture.

    abstract::In the context of cell-based oral hard tissue regeneration, especially assumed plasticity of oral host tissue cells in response to human mesenchymal stem cells (hMSCs), is poorly understood. To investigate this area, we assess osteogenic features in various oral cell types during hMSC coculture, including human alveol...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Proksch S,Steinberg T,Vach K,Hellwig E,Tomakidi P

    更新日期:2014-04-01 00:00:00

  • Precocene-induced necrosis and haemocyte-mediated breakdown of corpora allata in nymps of the locust Locusta migratoria.

    abstract::Light and electron microscopic studies, including the use of the vital dyes trypan blue and acridine orange, indicate that the topical application of precocene II rapidly triggers degenerative processes in the corpora allata (CA) leading within a few days to the virtual disappearance of the parenchymal cells of these ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schooneveld H

    更新日期:1979-11-01 00:00:00

  • Hypertrophy of radial and ulnar arteries following denervation of the chicken wing.

    abstract::Denervation of radial and ulnar arteries in the growing and adult domestic fowl was achieved by unilateral sectioning of the brachial plexus. Eight weeks later the denervated arteries and those of the contralateral wing were examined with light- and electron microscopy to determine the effect of denervation on arteria...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hartley L,Campbell GR

    更新日期:1987-12-01 00:00:00

  • Expression and localization of growth hormone and its receptors in the chicken ovary during sexual maturation.

    abstract::Roles of pituitary growth hormone (GH) in female reproduction are well established. Autocrine and/or paracrine actions of GH in the mammalian ovary have additionally been proposed, although whether the ovary is an extra-pituitary site of GH expression in the laying hen is uncertain. This possibility has therefore been...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hrabia A,Paczoska-Eliasiewicz HE,Berghman LR,Harvey S,Rzasa J

    更新日期:2008-05-01 00:00:00

  • Development of adrenal chromaffin cells in Sf1 heterozygous mice.

    abstract::Adrenal medullary chromaffin cells are derivatives of the neural crest and are widely believed to share a common sympathoadrenal (SA) progenitor with sympathetic neurons. For decades, the adrenal cortical environment was assumed to be essential for channelling SA progenitors towards an endocrine chromaffin cell fate. ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Lohr J,Gut P,Karch N,Unsicker K,Huber K

    更新日期:2006-09-01 00:00:00

  • Interdigitating cells in the lymphoid tissues of bovine fetuses and calves. An electron-microscopic study.

    abstract::Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bielefeldt Ohmann H,Basse A

    更新日期:1984-01-01 00:00:00

  • The retina-lamina projection in the visual system of the bee, Apis mellifera.

    abstract::Single Golgi impregnated visual cells and their axons were treated from the retina to the first synaptic layer (lamina) in serial electron microscopic sections. This analysis of the retina-lamina projection was undertaken in the upper dorso-median eye region which is known to be involved in the perception of polarized...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sommer EW,Wehner R

    更新日期:1975-11-17 00:00:00

  • Thy-1 is an in vivo and in vitro marker of liver myofibroblasts.

    abstract::Thy-1, a glycophosphatidylinositol-linked glycoprotein of the outer membrane leaflet, has been described in myofibroblasts of several organs. Previous studies have shown that, in fetal liver, Thy-1 is expressed in a subpopulation of ductular/progenitor cells. The aim of this study has been to investigate whether the l...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dudas J,Mansuroglu T,Batusic D,Saile B,Ramadori G

    更新日期:2007-09-01 00:00:00

  • The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions.

    abstract::N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pollerberg GE,Burridge K,Krebs KE,Goodman SR,Schachner M

    更新日期:1987-10-01 00:00:00

  • Growth and differentiation of murine cartilage cells in vitro following a short-term exposure to triamcinolone acetonide.

    abstract::Measurements of 3H-thymidine incorporation, quantitative autoradiography and morphometry were used to evaluate cell behavior during the recovery of mandibular condylar cartilage cultures following short-term exposure to a corticosteroid hormone in vitro. Apical segments of mandibular condyles of newborn mice were init...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Weiss A,Livne E,Silbermann M

    更新日期:1990-05-01 00:00:00

  • Lithium alleviated spinal cord injury (SCI)-induced apoptosis and inflammation in rats via BDNF-AS/miR-9-5p axis.

    abstract::Spinal cord injury (SCI) is a major cause of paralysis, disability and even death in severe cases. Lithium has neuroprotective effects on SCI, while the underlying mechanisms remain obscure. In the present study, we established a SCI rat model, which subsequently received lithium treatment. Results displayed that lith...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Wang F,Chang S,Li J,Wang D,Li H,He X

    更新日期:2021-01-19 00:00:00

  • Elimination of phagocytic cells in the spleen after intravenous injection of liposome-encapsulated dichloromethylene diphosphonate. An enzyme-histochemical study.

    abstract::Dichloromethylene diphosphonate can be used for temporary elimination of macrophages in the spleen when administered after entrapment in liposomes. No comparable effect on the macrophages of the spleen was observed with free dichloromethylene diphosphonate or in the case of empty liposomes. Marginal metallophils on th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: van Rooijen N,van Nieuwmegen R

    更新日期:1984-01-01 00:00:00