Abstract：
:Bovine granulosa cells from 3-7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30-100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antral follicles. Thus, granulosa cells in anchorage-independent cultures have a follicular cell morphology and secrete two distinct extracellular matrices, one similar to the follicular basal lamina.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Rodgers HF,Lavranos TC,Vella CA,Rodgers RJdoi
10.1007/BF00318878subject
Has Abstractpub_date
1995-12-01 00:00:00pages
463-71issue
3eissn
0302-766Xissn
1432-0878journal_volume
282pub_type
杂志文章abstract：:Kruppel-like factor 4 (KLF4) is a zinc-finger-type transcription factor with a restricted expression pattern during skeletal development. We have previously shown that KLF4 represses osteoblast mineralization concomitant with a down-regulation in the expression of a number of osteoblastic genes, both in vivo and in vi...
journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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更新日期：1979-06-08 00:00:00
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journal_title：Cell and tissue research
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更新日期：1995-10-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2000-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1997-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 历史文章,杂志文章
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更新日期：1995-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2005-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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更新日期：2011-06-01 00:00:00
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更新日期：2021-01-18 00:00:00
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pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1996-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1980-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
abstract：:Cyclooxygenase-2 (COX-2) is a recently discovered isoform of cyclooxygenase that is inducible by various types of inflammatory stimuli. Although this enzyme is considered to play a major role in inflammation processes by catalyzing the production of prostaglandins, the precise location, distribution, and regulation of...
journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
