Abstract:
:Cyclooxygenase-2 (COX-2) is a recently discovered isoform of cyclooxygenase that is inducible by various types of inflammatory stimuli. Although this enzyme is considered to play a major role in inflammation processes by catalyzing the production of prostaglandins, the precise location, distribution, and regulation of prostaglandin synthesis remains unclear in several tissues. Using in situ hybridization histochemistry, we investigated the induction of COX-1 and COX-2 mRNA expression after systemic administration of a pyrogen, lipopolysaccharide (LPS), in kidney and adrenal gland in the rat. The COX-2 mRNA signals dramatically increased 1 h after LPS treatment in the kidney outer medulla and adrenal cortex, where almost no or little expression was observed in nontreated animals, and returned to control levels within 24 h. COX-2 mRNA levels increased in the kidney inner medulla 6 h after treatment. There was also a significant increase in mRNA levels in the kidney cortex and adrenal medulla. On the other hand, COX-1 mRNA levels did not show any detectable changes except in the kidney inner medulla, where a significant downregulation of mRNA expression was observed after LPS treatment. Light and electron immunocytochemistry using COX-2 antibodies showed that strong COX-2 immunoreactivity was localized to certain cortical cells of the thick ascending limb of Henle. In addition, based on double-staining with antiserum to nitric oxide synthase (NOS) four further cell populations could be identified in kidney cortex, including weakly COX-2-positive, NOS-positive macula densa cells. After LPS treatment, changes in COX-2 immunoreactivity could be observed in interstitial cells in the kidney medulla and in inner cortical cells in the adrenal gland. These results show that COX-2 is a highly induced enzyme that can be up-regulated in specific cell populations in kidney and adrenal gland in response to inflammation, leading to the elevated levels of prostaglandins seen during fever. In contrast COX-1 mRNA levels remained unchanged in this experimental situation, except for a decrease in kidney inner medulla.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Ichitani Y,Holmberg K,Maunsbach AB,Haeggström JZ,Samuelsson B,De Witt D,Hökfelt Tdoi
10.1007/s004410000296keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
235-52issue
2eissn
0302-766Xissn
1432-0878journal_volume
303pub_type
杂志文章abstract::Acute necrotizing pancreatitis (ANP) is a common gastrointestinal cause of emergency admissions in dogs and humans and can lead to a systemic inflammatory response syndrome resulting in multiple organ dysfunction syndrome. Among the various complications associated with ANP, acute lung injury (ALI) or acute respirator...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03211-y
更新日期:2020-05-01 00:00:00
abstract::The thoracic legs of the moth Manduca sexta acquire a new form and develop a new complement of sensory organs and muscles during metamorphosis from larva to adult. Because of our interest in the reorganization of neural circuitry and the acquisition of new behaviors during metamorphosis, we are characterizing sensory ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318442
更新日期:1990-02-01 00:00:00
abstract::Previously, we showed that synchronous autophagy occurred in the corpora allata during the declining phase of juvenile hormone synthesis in mated adult females of the cockroach Diploptera punctata. At the onset of this process, conspicuous whorl-like autophagosomes were often observed. Each consisted of a ribosome-con...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050764
更新日期:1997-02-01 00:00:00
abstract::This review summarizes findings concerning the unique developmental characteristics of mouse head muscles (mainly the masticatory and tongue muscles) and compares their characteristics with those of other muscles. The developmental origin of the masticatory muscles is the somitomeres, whereas the tongue and other musc...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-005-0019-x
更新日期:2005-11-01 00:00:00
abstract::The present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. A marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00318822
更新日期:1994-03-01 00:00:00
abstract::The high affinity IgE receptor on mast cells and basophils is composed of 3 subunits, alpha, beta and gamma. A polyclonal antibody was raised in rabbits to a 13 amino acid synthetic peptide corresponding to the amino terminal portion of the gamma subunit. The antiserum was screened using Western blots of solubilized R...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410050575
更新日期:1996-04-01 00:00:00
abstract::Honeybees are extensively used to study olfactory learning and memory processes thanks to their ability to discriminate and remember odors and because of their advantages for optophysiological recordings of the circuits involved in memory and odor perception. There are evidences that the encoding of odors in areas of ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-020-03396-2
更新日期:2021-01-29 00:00:00
abstract::iASPP (inhibitory member of the ASPP family), the conserved key inhibitor of p53, is overexpressed and plays an important oncogenetic role in many human cancers. However, its function in cervical cancer remains unknown. The objective of this study was to investigate the expression and clinical relevance of iASPP in ce...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1569-y
更新日期:2013-05-01 00:00:00
abstract::Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2931-x
更新日期:2019-03-01 00:00:00
abstract::An ultrastructural study of the rostral pars distalis of the pituitary of Aphanius dispar specimens taken from freshwater or hypersaline marshes revealed significant structural differences which indicate higher activity of the prolactin cells in the hypotonic medium. Prolactin cells from freshwater specimens had large...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221103
更新日期:1977-04-20 00:00:00
abstract::Effects of prolactin on the secretion of milk proteins have been investigated by incubating mammary tissue fragments from lactating rabbits. Within 15 min of adding the hormone to the incubation medium, cell morphology is modified: the relative volume occupied by the Golgi region is greatly increased. When prolactin i...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220616
更新日期:1978-02-14 00:00:00
abstract::Interstitial cells of Cajal (ICC) are important regulatory cells in the smooth muscle coats of the digestive tract. Expression of the Kit receptor tyrosine kinase was used in this study as a marker to study their distribution and development in the striated musculature of the mouse esophagus. Sections and whole-mounts...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410100418
更新日期:2001-10-01 00:00:00
abstract::The uptake of macromolecules and larger energy-rich particles into the cell is known as phagocytosis. Phagocytosed material is enzymatically degraded in membrane-bound vesicles of the endosome/lysosome system (intracellular digestion). Whereas most, if not all, cells of the animal body are equipped with the molecular ...
journal_title:Cell and tissue research
pub_type: 杂志文章,评审
doi:10.1007/s00441-019-03096-6
更新日期:2019-09-01 00:00:00
abstract::When stimulated by the feeding of ethylenethiourea for a period of 6 months, dog thyroid glands increased to approximately 30 times their normal size. Not only follicular cells but also C cells had hyperplastic and hypertrophic features, compared with normal controls there was in the C cells a marked decrease in secre...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00214684
更新日期:1982-01-01 00:00:00
abstract::Ontogenesis comprises a series of events including cell proliferation and apoptosis and resulting in the normal development of the embryo. Protein p53 has been described as being involved in the development of several animal species. The aim of this study was to analyze the expression of protein p53 during the morphog...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1720-9
更新日期:2014-01-01 00:00:00
abstract::DNA distribution in mouse, rabbit and bull spermatids was analyzed by electron microscopy, after using a Feulgen-like HCl-osmium ammine procedure, and after immunocytochemistry with anti-DNA antibodies. In addition, nucleic acids were visualized with the intercalating dye ethidium bromide and phosphotungstic acid. The...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00340875
更新日期:1991-09-01 00:00:00
abstract::Antisera to the neuropeptides corazonin (Crz) and crustacean cardioactive peptide (CCAP) and to the diapause hormone (DH) react with small sets of neurones in the cephalic ganglia of the crickets Dianemobius nigrofasciatus and Allonemobius allardi. The distribution of their immunoreactivities is similar in the two spe...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-006-0339-5
更新日期:2007-04-01 00:00:00
abstract::The clear zones seen around microtubules in transverse sections of nutritive tubes vary in size depending on whether a microtubule is bordered by ribosomes or by another microtubule. We consider that such a finding is not consistent with the current view, that the clear zone is maintained by microtubule-associated mat...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00204791
更新日期:1982-01-01 00:00:00
abstract::Antithrombin (AT), which is mainly synthesized in the liver, is an acute-phase plasma protein in mammalian species. Here, we demonstrated that sheep anti-human AT antibody cross-reacted with the humoral fluids in amphioxus Branchiostoma belcheri tsingtauense as well as human serum. The concentration of AT in the humor...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-0088-x
更新日期:2006-03-01 00:00:00
abstract::The distribution of cochlin and its associated basement membrane proteins (collagen IV, collagen II, laminin-β2, and nidogen-1) were evaluated in the vestibular endorgans of subjects with Meniere's disease and compared with normal specimens. Cochlin mRNA expression in vestibular endorgans from Meniere's disease specim...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-012-1481-x
更新日期:2012-11-01 00:00:00
abstract::As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled wi...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00233538
更新日期:1981-01-01 00:00:00
abstract::The formylpeptide receptor (FPR), previously found only on polymorphonuclear leukocytes and monocytes/macrophages, responds to both synthetic N-formyl oligopeptides and those produced by bacteria. The cDNA for human FPR has been cloned and a rabbit polyclonal antiserum directed against a synthetic 11-amino-acid peptid...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051042
更新日期:1998-04-01 00:00:00
abstract::Enzyme-histochemical methods were used to study the metabolic activity of specialized ependyma of the ventrolateral walls and floor of the third ventricle in young male and female rats during the "critical period" of sexual differentiation of the hypothalamus (one week after birth). Histochemical tests were conducted ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00220328
更新日期:1976-10-13 00:00:00
abstract::Purinoceptor subtypes were localised to various tissue types present within the nasal cavity of the rat, using immunohistochemical methods. P2X3 receptor immunoreactivity was localised in the primary olfactory neurones located both in the olfactory epithelium and vomeronasal organs (VNO) and also on subepithelial nerv...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-004-0979-2
更新日期:2005-01-01 00:00:00
abstract::Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-infla...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-018-2857-3
更新日期:2018-10-01 00:00:00
abstract::The lymphatic organization and its relationship to the vascular system in the rat small intestine was studied by scanning electron microscopy of corrosion casts and freeze-fractured tissues, and by light microscopy of injected preparations. The villus possessed 3-10 or more central lacteals depending upon the villous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218204
更新日期:1987-05-01 00:00:00
abstract::"White pigment cells" are derived from melanophore precursors and contain both melanophore-specific and iridophore-specific pigment organelles. Whereas melanophores differentiate in the wild type regenerating tail, white pigment cells appear in the regenerating tail in the periodic albino mutant (a(p)/a(p)) of Xenopus...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-015-2133-8
更新日期:2015-09-01 00:00:00
abstract::The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00222230
更新日期:1983-01-01 00:00:00
abstract::The retinal projections in adult and juvenile guitar fish (Rhinobatos productus) were determined with the aid of the Nauta-Fink-Heimer techniques. The visual system was found to be more extensive and more differentiated than in any other elasmobranch studied to date. Massive projections exist to the dorsal and ventral...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00232768
更新日期:1980-01-01 00:00:00
abstract::SCO-spondin is a newly identified protein, strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When secreted into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, it condenses and forms Reissner's fiber. Several conserved domains have previously been charact...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s004410051132
更新日期:1998-09-01 00:00:00