Abstract：
:Pituitary adenylate cyclase-activating polypeptide (PACAP) is the latest member of the vasoactive intestinal polypeptide (VIP) family of neuropeptides present in nerve fibres in many peripheral organs. Using double immunohistochemistry, with VIP as a marker for intrinsic innervation and calcitonin-gene related peptide (CGRP) as a marker for mainly extrinsic innervation, the distribution and localization of PACAP were studied in the rat pancreas. PACAP was demonstrated in nerve fibres in all compartments of the pancreas and in a subpopulation of intrapancreatic VIP-containing ganglion cells. PACAP and VIP were co-stored in intra- and interlobular nerve fibres innervating acini, blood vessels, and in nerve fibres within the islets of Langerhans. No PACAP immunoreactivity was observed in the islet cells. Another population of PACAP-immunoreactive nerve fibres co-localized with CGRP innervated ducts, blood vessels and acini. PACAP/CGRP-positive nerve fibres were also demonstrated within the islets. Neonatal capsaicin reduced the PACAP-38 concentration by approximately 50%, and accordingly a marked reduction in PACAP/CGRP-immunoreactive nerve fibres in the exocrine and endocrine pancreas was observed. Bilateral subdiaphragmatic vagotomy caused a slight but significant decrease in the PACAP-38 concentration compared with controls. In conclusion, PACAP-immunoreactive nerve fibres in the rat pancreas seem to have dual origin: extrinsic, most probably sensory fibres co-storing CGRP; and intrinsic, constituting a subpopulation of VIP-containing nerve cell bodies and fibres innervating acinar cells and islet cells. Our data provide a morphological basis for the reported effects of PACAP in the pancreas and suggest that PACAP-containing nerves in the rat pancreas may have both efferent and sensory functions.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Hannibal J,Fahrenkrug Jdoi
10.1007/s004419900124keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
59-70issue
1eissn
0302-766Xissn
1432-0878journal_volume
299pub_type
杂志文章abstract：:We have examined radioautographically the protein synthetic and secretory activity of differentiating odontoblasts and ameloblasts, exposed for 9 h in vitro to various concentrations of colchicine in the presence of 3H-proline. Colchicine impairs the cytodifferentiation of the dental epithelium into ameloblasts and of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216651
更新日期：1988-06-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050531
更新日期：1996-02-01 00:00:00
abstract：:Cytochemical methods for the demonstration of p-nitrophenylphosphatase (p-NPPase) at the electron-microscopic level were applied to avian osteoclasts to elucidate some of the functional differences among ruffled border, other membrane systems, and cellular organelles. The localization of p-NPPase activity occurred in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218381
更新日期：1986-01-01 00:00:00
abstract：:We conducted an immunoelectron-microscopic study for histamine (HA) in the enterochromaffin-like (ECL) cells of normal rats and rats given alpha-fluoromethylhistidine (alpha-FMH, 3 mg/kg per hour) via osmotic minipumps over a period of 24 h. The indirect immunoperoxidase procedure utilized a mouse monoclonal antibody ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100448
更新日期：2001-11-01 00:00:00
abstract：:The development of the rat adrenal medulla was studied at the ultrastructural level with particular emphasis placed on early discrimination of different catecholamine-storing cells. The first granule-containing cells, phaeochromoblasts, were seen at day 15 of gestation migrating into the anlage of the cortex. These ce...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233834
更新日期：1981-01-01 00:00:00
abstract：:Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051013
更新日期：1998-03-01 00:00:00
abstract：:With the use of tissue prepared by freeze-substitution and the unlabelled antibody enzyme technique, neurophysin and vasopressin were localized at the ultrastructural level in the pofterior pituitary and median eminence of the guinea pig. In the posterior pituitary neurophysin was found in the large neurosecretory gra...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221777
更新日期：1975-06-13 00:00:00
abstract：:During developmental critical periods, external stimuli are crucial for information processing, acquisition of new functions or functional recovery after CNS damage. These phenomena depend on the capability of neurons to modify their functional properties and/or their connections, generally defined as "plasticity". Al...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-011-1293-4
更新日期：2012-07-01 00:00:00
abstract：:Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with highly potent neurotrophic and neuroprotective effects. PACAP and its receptors occur in the retina and PACAP has been applied in animal models of metabolic retinal disorders to reduce structural and functional damage. Furthermore, PACAP...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1349-0
更新日期：2012-04-01 00:00:00
abstract：:A special cell type was identified in the mucosal epithelium of the large intestine of the tadpole of the bullfrog, Rana catesbeiana. It is a slender, columnar cell, with a dark, basally situated nucleus. By electron microscopy the cell displays prominent bundles of filaments emerging from each microvillus and extendi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00213817
更新日期：1983-01-01 00:00:00
abstract：:Voltage-gated Na+ channels are expressed by highly metastatic MAT-LyLu cells, but not by poorly metastatic AT-2 cells, derived from the rodent Dunning model of prostatic cancer. We have investigated the possible involvement of these channels in the morphological development of the cells. Incubation of both the MAT-LyL...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051256
更新日期：1999-03-01 00:00:00
abstract：:The desert locust Schistocerca gregaria is a major agricultural pest in North Africa and the Middle East. As such, it has been intensely studied, in particular with respect to population dynamics, sensory processing, feeding behavior flight and locomotor control, migratory behavior, and its neuroendocrine system. Bein...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2844-8
更新日期：2018-10-01 00:00:00
abstract：:Synaptogyrins are conserved components of the exocytic apparatus and function as regulators of Ca(2+)-dependent exocytosis. The synaptogyrin family comprises three isoforms: two neuronal (synaptogyrin-1 and -3) and one ubiquitous (synaptogyrin-2) form. Although the expression patterns of the exocytic proteins synaptot...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-013-1629-3
更新日期：2013-09-01 00:00:00
abstract：:Steroid hormones intervene in the structural and functional regulation of neuronal processes during development and thus determine brain differentiation. The effects of estrogens are mediated by two transcription factors, namely estrogen receptor α (ER-α) and estrogen receptor β (ER-β), that regulate the expression of...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-2023-5
更新日期：2015-02-01 00:00:00
abstract：:The retinal projections in adult and juvenile guitar fish (Rhinobatos productus) were determined with the aid of the Nauta-Fink-Heimer techniques. The visual system was found to be more extensive and more differentiated than in any other elasmobranch studied to date. Massive projections exist to the dorsal and ventral...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232768
更新日期：1980-01-01 00:00:00
abstract：:Galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. Nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. Lesion studies showe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218954
更新日期：1987-12-01 00:00:00
abstract：:Two dozen hybrid clones were produced by fusion of diploid embryonic stem (ES) cells positive for green fluorescent protein (GFP) with tetraploid fibroblasts derived from DD/c and C57BL-I(I)1RK mice. Cytogenetic analysis demonstrated that most cells from these hybrid clones contained near-hexaploid chromosome sets. Ad...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0987-3
更新日期：2010-06-01 00:00:00
abstract：:The effect of pilocarpine and food uptake on the rate of incorporation of [3H]-leucine in vivo was measured by means of quantitative radioautography in three exocrine cells of the rat: the acinar and the granular duct cells of the submandibular and the acinar cells of the parotid gland. The three cell types react diff...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219936
更新日期：1975-12-18 00:00:00
abstract：:Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03044-4
更新日期：2019-11-01 00:00:00
abstract：:The application of autologous dermal fibroblasts has been shown to improve burn wound healing. However, a major hurdle is the availability of sufficient healthy skin as a cell source. We investigated fetal dermal cells as an alternative source for cell-based therapy for skin regeneration. Human (hFF), porcine fetal (p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2293-6
更新日期：2016-04-01 00:00:00
abstract：:Neuroblastoma is an embryonal malignancy that affects normal development of the adrenal medulla and paravertebral sympathetic ganglia in early childhood. Extensive studies have revealed the molecular characteristics of human neuroblastomas, including abnormalities at genome, epigenome and transcriptome levels. However...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-018-2796-z
更新日期：2018-05-01 00:00:00
abstract：:The phase of primitive erythropoiesis in the feline yolk sac lasts from the 14th to the 20th day after malting. The globular nucleated primitive erythroblasts are formed extravascularly to some extent, but they can be clearly distinguished from the endoderm. They do not undergo a denucleation and are still present in ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219993
更新日期：1977-09-14 00:00:00
abstract：:The reduced-folate carrier (Rfc-1), previously also called methotrexate carrier-1 (MTX-1), was recently identified as accounting for approximately 30% of the methotrexate (Mtx) uptake into rat kidney slices. The localization of the carrier and its contribution to secretory or reabsorptive flux of the drug was therefor...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-005-1092-x
更新日期：2005-06-01 00:00:00
abstract：:This review focuses on the signaling system involving the Angiopoietin1/Tie2 receptor, which appears to be involved in the secondary stages of blood vessel formation. Although this system is crucial for blood and lymphatic vessel formation, identifying its precise role in embryonic and adult vascular biology has been ...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-003-0749-6
更新日期：2003-10-01 00:00:00
abstract：:Interstitial cells in the pineal gland of the rat were characterized immunocytochemically using the monoclonal antibodies MRC OX-42 and ED1 for macrophages/microglia, and MRC OX-6, which recognizes major histocompatibility complex (MHC) class II antigen. A polyclonal antibody against GFAP was used to identify astrocyt...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302731
更新日期：1993-05-01 00:00:00
abstract：:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214574
更新日期：1985-01-01 00:00:00
abstract：:Scanning electrom microscopy of the ventricular surface of the pars ventralis of the tuber cinereum of the frog Rana temporaria shows that it can be divided in (1) a dorsolateral area, characterized by the presence of very numerous large, solid, bulbous protrusions (2) a medial area, where the large bulbous structures...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00231997
更新日期：1975-05-27 00:00:00
abstract：:The association between adipose tissue and immunity has been established and fat-associated lymphoid clusters (FALCs) are considered as a source of immune cells. We discovered lymphoid clusters (LCs) in mouse mediastinal fat tissues (MFTs). In Th1-biased C57BL/6N (B6), Th2-biased DBA/2Cr (DBA) and autoimmune-prone MRL...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1889-6
更新日期：2014-09-01 00:00:00
abstract：:Neurosecretory pathways were examined in normal male rats by the use of the immunoperoxidase technique for the localization of neurophysin in Bouin-fixed, deparaffinized sections. Using this technique two projections of extrahypothalamic neuorosecretory fibers can be traced to the sites of origin of the choid plexuses...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00232829
更新日期：1977-03-01 00:00:00
