Presence and localization of aquaporin-6 in rat parotid acinar cells.


:Aquaporins (AQPs) are integral membrane proteins that function as channels for the transfer of water and small solutes across membranes. In mammalian cells, 13 isoforms (AQP0-12) have been identified, and these exhibit unique patterns of expression in various cell types and tissues. Among these isoforms, AQP6 is considered to function not as water channel, but as an anion channel. We investigated the presence and localization of AQP6 in rat parotid acinar cells. AQP6 mRNA was detected in these cells by using reverse transcription/polymerase chain reaction, and Western blotting analysis identified a protein band that reacted with an anti-AQP6 antibody in the membrane fraction and secretory granule membrane. In order to localize AQP6, we used the anti-AQP6 antibody for histological immunodetection. Under confocal microscopy, we observed positive immunoreactions near the tight junctions of parotid acinar cells. Immunolabeling of ultrathin cryosections detected AQP6 near tight junctions and around secretory granule membranes. Immunoelectron microscopy confirmed the presence of AQP6 in the membranes of isolated secretory granules. These results suggest that AQP6 participates in water and anion transport in plasma membranes near tight junctions and secretory granule membranes in rat parotid acinar cells.


Cell Tissue Res


Cell and tissue research


Matsuki-Fukushima M,Hashimoto S,Shimono M,Satoh K,Fujita-Yoshigaki J,Sugiya H




Has Abstract


2008-04-01 00:00:00












  • Cell differentiation of the fetal rat anterior pituitary in vitro.

    abstract::Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Nemeskéry A,Németh A,Sétáló G,Vigh S,Halász B

    更新日期:1976-07-26 00:00:00

  • Comparison of the osteogenic potentials of autologous cultured osteoblasts and mesenchymal stem cells loaded onto allogeneic cancellous bone granules.

    abstract::We compared the bone regeneration potentials of autologous cultured osteoblasts and of bone-marrow-derived autologous MSCs in combination with allogeneic cancellous bone granules in a rabbit radial defect model. Radial shaft defects over 15 mm were made in 26 New Zealand white rabbits. The animals underwent insertion ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kim SJ,Chung YG,Lee YK,Oh IW,Kim YS,Moon YS

    更新日期:2012-02-01 00:00:00

  • Seasonal changes in the fine structure of the accessory sex gland in the mole (Talpa europaea).

    abstract::The mole has a single pair of accessory sex organs with features of both the prostate and the seminal vesicle, for which the term prostate gland is not appropriate. Seasonal changes occuring in this gland were related to four periods: a) the quiescence period, b) the maturation period, c) the active period and d) the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Aumüller G,Greenberg J

    更新日期:1976-12-10 00:00:00

  • Cytochemical variations in the nucleolus during spermiogenesis in man and monkey.

    abstract::The fine structure, nature and fate of the components of the nucleolus were studied in young (steps 1, 2), intermediate (steps 3, 4, 5) and mature spermatids (steps 6, 7, 8) of man and monkey, by use of several cytochemical techniques (alcoholic PTA; sodium tungstate: EDTA; HAPTA; nuclease-gold complexes; NOR silver s...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Dadoune JP,Alfonsi MF,Fain-Maurel MA

    更新日期:1991-04-01 00:00:00

  • Ultrastructural alterations of the pancreatic D cell in the domestic fowl following vagotomy.

    abstract::In an attempt to determine the neural control of pancreatic D cells, the pancreatic islets of the domestic fowl were examined electron microscopically from 1 to 28 days after abdominal vagotomy. Exocytotic release of many secretory granules from D cells occurred one day after vagotomy. Rough endoplasmic reticulum deve...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Watanabe T,Fujioka T

    更新日期:1980-01-01 00:00:00

  • Surface fine structure of the globiferous pedicellariae of the regular echinoid, Psammechinus miliaris Gmelin.

    abstract::The globiferous pedicellariae of Psammechinus miliaris are described. Two fixation methods giving minimal distortion and rapid tissue hardening were adapted for soft tissue preparation for scanning electron microscopy. The pedicellarial valves are covered by a microvillous epithelium. The outer valve epithelial microv...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Oldfield SC

    更新日期:1975-10-13 00:00:00

  • Expression of urocortin and corticotropin-releasing hormone receptors in the horse thyroid gland.

    abstract::Urocortin (UCN) is a 40-amino-acid peptide and a member of the corticotropin-releasing hormone (CRH) family, which includes CRH, urotensin I, sauvagine, UCN2 and UCN3. The biological actions of CRH family peptides are mediated via two types of G-protein-coupled receptors, namely CRH type 1 receptor (CRHR1) and CRH typ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Squillacioti C,De Luca A,Alì S,Paino S,Liguori G,Mirabella N

    更新日期:2012-10-01 00:00:00

  • Retrograde labeling of regenerated electromotor neurons with HRP in a teleost fish, Sternarchus albifrons: relation to cell death.

    abstract::Back-labeling of regenerated electromotor neurons in the teleost Sternarchus albifrons was performed to test the hypothesis that, in regenerated spinal cord, incorrectly located electromotor neurons are eliminated because their axons do not reach the correct target area (electric organ). In each cross section examined...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Anderson MJ,Fong HL,Waxman SG

    更新日期:1985-01-01 00:00:00

  • Concanavalin A-binding glycoproteins in the subcommissural and the pineal organ of the sheep (Ovis aries). A fluorescence-microscopic and electrophoretic study.

    abstract::Glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (SCO) and the pineal organ of the sheep (Ovis aries). By use of concanavalin A labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the SCO. In the pineal organ, e...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Meiniel R,Molat JL,Meiniel A

    更新日期:1986-01-01 00:00:00

  • Choline acetyltransferase immunocytochemical staining of the rat supraoptic nucleus and its surroundings. A light- and electron-microscopic study.

    abstract::Two different monoclonal antibodies raised against choline acetyltransferase were used, together with preembedding immunocytochemical techniques, to visualize the possible cholinergic innervation of the supraoptic and paraventricular nuclei of the rat hypothalamus. Light microscopy confirmed the presence of a group of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Theodosis DT,Mason WT

    更新日期:1988-10-01 00:00:00

  • An electron-microscopic study of the bone-remodeling sequence in the rat.

    abstract::A detailed chronological electron-microscopic study of the bone remodeling sequence has been performed in the rat based on a previously described model (Tran Van et al. 1982) in which the remodeling activity is synchronized. This allowed the observation of the cellular and extracellular events during the bone remodeli...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Tran Van P,Vignery A,Baron R

    更新日期:1982-01-01 00:00:00

  • The influence of androgen administration on the structure and function of the brain-pituitary-gonad axis of sexually immature platyfish, Xiphophorus maculatus.

    abstract::This report demonstrates that the administration of testosterone (T) or 11-ketotestosterone (11-KT) to sexually immature (8 wks old) male platyfish (Xiphophorus maculatus) of early- and late-maturing genotypes affects the synthesis and/or release of luteinizing hormone-releasing hormone (LHRH), as assessed by immunocy...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Schreibman MP,Margolis-Nunno H,Halpern-Sebold LR,Goos HJ,Perlman PW

    更新日期:1986-01-01 00:00:00

  • Variation in serotonergic and dopaminergic neuronal survival in the central nervous system of adult Drosophila.

    abstract::Loss of serotonergic and dopaminergic neurons may have serious implications for normal brain function. Drosophila models of neurodegenerative diseases utilize the short life-span and simple anatomy of the fly to characterize the molecular and genetic processes characteristic of each dysfunctional state. In fly embryon...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Sykes PA,Norman HS,Condron BG

    更新日期:2004-09-01 00:00:00

  • Effect of salt loading and salt deprivation on the vasopressin and oxytocin content of the median eminence and the neural lobe in adrenalectomized rats.

    abstract::In adrenalectomized rats the influence of salt loading or salt deprivation on the vasopressin and oxytocin content of the median eminence (ME) and the neural lobe (NL) was studied by means of various methods: morphometric and microphotometric analysis of aldehyde fuchsin-stained sections of ME and NL; immunohistochemi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Mink D,Lang RE,Ostermann E,Bock R

    更新日期:1986-01-01 00:00:00

  • Morphological and functional changes in the tight junctions of the bile canaliculi induced by bile duct ligation.

    abstract::Thin sections after bile duct ligation showed that the depth of tight junctions appeared to increase and that the distance between individual punctate contacts appeared to become irregular and wider than in controls. The freeze fracture replicas clearly demonstrated these changes in the tight junction morphology. Chan...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Koga A,Todo S

    更新日期:1978-12-28 00:00:00

  • Gene expression profiles of estrogen receptors α and β in the fetal bovine hypothalamus and immunohistochemical characterization during development.

    abstract::Steroid hormones intervene in the structural and functional regulation of neuronal processes during development and thus determine brain differentiation. The effects of estrogens are mediated by two transcription factors, namely estrogen receptor α (ER-α) and estrogen receptor β (ER-β), that regulate the expression of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Panin M,Corain L,Montelli S,Cozzi B,Peruffo A

    更新日期:2015-02-01 00:00:00

  • Basic principles and molecular mechanisms of olfactory axon pathfinding.

    abstract::The present review describes several lines of recent evidence providing new insights into the basic principles and mechanisms of axon projection from the olfactory epithelium to the olfactory bulb. Olfactory sensory neurons are classified into approximately 1000 subtypes according to the expression of specific odorant...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Yoshihara Y,Mori K

    更新日期:1997-11-01 00:00:00

  • Genetic and epigenetic control of muscle development in vertebrates.

    abstract::The skeletal body muscle of vertebrates is derived from segmentally arranged mesodermal structures, the somites. Only the dorsal epithelial half of the somite, the dermomyotome, gives rise to muscle cells during normal development. Head muscle takes its origin from the somites, the unsegmented paraxial head mesoderm a...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Brand-Saberi B,Christ B

    更新日期:1999-04-01 00:00:00

  • Complex relationships between the pineal organ and the medial habenular nucleus-pretectal region of the mouse as revealed by S-antigen immunocytochemistry.

    abstract::S-antigen-immunoreactive pinealocytes located in the deep portion of the pineal organ of inbred and wild pigmented mice give rise to long, beaded processes penetrating into the habenular and pretectal regions. In addition, the medial habenular nuclei and the pretectal area contain S-antigen-immunoreactive perikarya, w...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Korf HW,Sato T,Oksche A

    更新日期:1990-09-01 00:00:00

  • The mouse pubic symphysis as a remodeling system: morphometrical analysis of proliferation and cell death during pregnancy, partus and postpartum.

    abstract::Marked changes in mice pubic symphysis occur by the end of pregnancy. Tissue remodeling involves a dynamic balance between cell proliferation and programmed cell death as well as changes in the extracellular matrix components. Therefore, it is important to consider both of these cellular behaviors when investigating t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Veridiano AM,Garcia EA,Pinheiro MC,Nishimori FY,Toledo OM,Joazeiro PP

    更新日期:2007-10-01 00:00:00

  • Actin organization and fibrin-clot retractile activity of cultured mouse fibroblasts.

    abstract::It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Curatolo L,Chaponnier C,Donati MB,Morasca L,Gabbiani G

    更新日期:1982-01-01 00:00:00

  • Carbamylcholine-induced morphological changes and spatial dynamics of [Ca2+]c in Harderian glands of guinea pigs: calcium-dependent lipid secretion and contraction of myoepithelial cells.

    abstract::To determine whether lipid-secreting cells have cytosolic Ca2+ concentration ([Ca2+]c)-related secretory mechanisms, morphological changes and intracellular calcium dynamics of Harderian glands of guinea pigs stimulated by secretagogue were studied by electron microscopy and Fura-2/AM digital image analysis. Control g...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Satoh Y,Habara Y,Kanno T,Ono K

    更新日期:1993-10-01 00:00:00

  • Immunocytochemical evidence of plasticity in the nervous structures of the rat lower lip.

    abstract::In this immunocytochemical study we investigated the distribution of nervous structures in the lower lip of adult rats. The region is characterized by a rich cutaneous and mucosal sensory innervation originating from terminal branches of the trigeminal system. Lower lip innervation was investigated by detection of the...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Verzé L,Paraninfo A,Ramieri G,Viglietti-Panzica C,Panzica GC

    更新日期:1999-08-01 00:00:00

  • The response of the satellite and other non-neuronal cells to the degeneration of neuroblasts in chick embryo spinal ganglia.

    abstract::The responses of non-neuronal cells to the degeneration of neuroblasts were studied in the spinal ganglia of the chick embryo. Reactive changes of the non-neuronal cells were not apparent during the early stage of degeneration of the neuroblast, but during the later stages of degeneration evidence was found suggesting...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pannese E

    更新日期:1978-06-26 00:00:00

  • Expression of alpha SM actin in terrestrial ectothermic vertebrates.

    abstract::alpha-Smooth muscle (alpha SM) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha SM-1. Immunoreactivity to this antibody has been shown to be localized in the NH2-terminal sequence Ac-EEED (Chaponnier et al. 1994). Among terrestrial ectothermic vertebrates, two amphibian ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Di Rosa I,Panara F,Fagotti A,Simoncelli F,Chaponnier C,Gabbiani G,Pascolini R

    更新日期:1995-09-01 00:00:00

  • Cytomorphological study on human submandibular gland following treatment with secretagogue drugs.

    abstract::Using specimens of human submandibular glands, we have investigated in vitro the morphological modifications induced by clozapine, a dibenzodiazepine derivative that is used in psychotic patients and that provokes hypersalivation, a side-effect of therapy. The effects of the drug, used alone or in combination with car...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Testa Riva F,Puxeddu R,Loy F,Conti G,Riva A

    更新日期:2006-05-01 00:00:00

  • The influence of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni (Cichlidae, Teleostei).

    abstract::The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Pietzsch-Rohrschneider I

    更新日期:1977-03-09 00:00:00

  • Distribution of subgroups of noradrenaline neurons in the coeliac ganglion of the guinea-pig.

    abstract::The distributions within the coeliac ganglion of different chemically coded subgroups of noradrenaline neurons, and the relationships between these neurons and nerve fibres projecting to the ganglion from the intestine, have been assessed quantitatively by use of an immunohistochemical double-staining method. Noradren...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Macrae IM,Furness JB,Costa M

    更新日期:1986-01-01 00:00:00

  • Increased intracellular content of enteroglucagon in proximal colon is related to intestinal adaptation to germ-free status.

    abstract::Changes in the frequency of endocrine cells are evidence of intestinal adaptation to germ-free (GF) status. Not only the distribution of these cells along the intestine, but also the differences in intracellular content of these regulatory peptides may be explored to explain functional and structural aspects of GF int...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Arantes RM,Nogueira AM

    更新日期:2001-03-01 00:00:00

  • Differentiation of human skeletal muscle cells in culture: maturation as indicated by titin and desmin striation.

    abstract::This report describes a phenotyping study of differentiating human skeletal muscle cells in tissue culture. Satellite cells (adult myoblasts), isolated from biopsy material, showed a proliferative behaviour in high-nutrition medium, but fused to form myotubes when grown in low-nutrition medium. The expression and stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: van der Ven PF,Schaart G,Jap PH,Sengers RC,Stadhouders AM,Ramaekers FC

    更新日期:1992-10-01 00:00:00