Abstract：
:Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this technique by quantifying immunostained pulmonary nuclei. We applied polyclonal and monoclonal antisera to alveolar and bronchiolar pulmonary epithelial cells in various proliferative states in tissue-sections and in vitro. A/J mice had a slightly higher labeling index than C57BL/6J mice, and proliferation in both strains increased dramatically after butylated hydroxytoluene treatment produced compensatory hyperplasia of Type-II pneumocytes. Immunostaining in fetal and neonatal lung samples from mice was higher than in adults. Spontaneous lung adenomas had a higher labeling index than the surrounding normal lung tissue. In addition, new data contained herein demonstrate a strain difference in proliferation of bronchiolar epithelial cells, and quantify the extent to which BHT-induced lung damage increases these proliferative rates. This mammalian nuclear antigen did not cross-react with antiserum to a functionally related bacterial protein, the beta subunit of E. coli DNA polymerase-III holoenzyme.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Thaete LG,Ahnen DJ,Malkinson AMdoi
10.1007/BF00224731subject
Has Abstractpub_date
1989-04-01 00:00:00pages
167-73issue
1eissn
0302-766Xissn
1432-0878journal_volume
256pub_type
杂志文章abstract：:Neural lobes of rats subjected to dehydration by drinking 2% saline for four days were examined electron microscopically and compared to untreated controls. The ultrastructure of the blood vessels and the tissues surrounding them were examined and it was found that, although few exocytotic figures could be seen in eit...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221710
更新日期：1975-06-24 00:00:00
abstract：:The rejection of skin allografts by the larval lamprey, Lampetra reissneri, was studied by light- and electron-microscopy, with particular attention to the cell types involved in the reaction. In all allografts, melanophores were destroyed within 20-60 days (the mean survival time, 36 +/- 12 days). Neither the epiderm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222182
更新日期：1983-01-01 00:00:00
abstract：:The distribution of gap junctions (nexuses) in various parts of the small and large intestines of the guinea-pig was studied using the freeze-fracture technique and in thin sections. The percentage area of smooth muscle cell surface occupied by gap junctions varies from 0.50% in the circular muscle of the duodenum to ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209987
更新日期：1981-01-01 00:00:00
abstract：:Two different fixative procedures (immersion and perfusion) and four different fixative solutions were used in order to obtain the best preservation of the brush cells of the common bile duct of the rat. The results indicate that only perfusion fixation through the common bile duct is suitable, independent of the fixa...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210353
更新日期：1981-01-01 00:00:00
abstract：:Neuronal differentiation is characterized by stereotypical sequences of membrane channel and receptor acquisition. This is regulated by the coordinated interactions of a variety of developmental mechanisms, one of which is the control by steroid hormones. We have used the metamorphosis of the holometabolous insect, Ma...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0175-7
更新日期：2006-07-01 00:00:00
abstract：:Rat thymic phagocytic cells were characterized in vitro using various light- and electron-microscopical techniques. Thymic cell suspensions were mechanically prepared and enriched for non-lymphoid cells, which were predominantly phagocytic and of three types. Type I showed acid phosphatase (APh) activity in small gran...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222183
更新日期：1983-01-01 00:00:00
abstract：:An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone ahd no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216742
更新日期：1981-01-01 00:00:00
abstract：:DBA/2J (D2) mice develop a form of progressive pigmentary glaucoma with increasing age. We have compared retinal cell populations of D2 mice with those in control C57BL/6J mice to provide information on retinal histopathology in the D2 mouse. The D2 mouse retina is characterized by a reduction in retinal thickness cau...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-1062-8
更新日期：2005-04-01 00:00:00
abstract：:Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0668-7
更新日期：2008-10-01 00:00:00
abstract：:Neurons projecting from the brain to the pineal gland via the pineal stalk were investigated in the golden hamster with the use of the retrograde horseradish-peroxidase tracing method both in vivo and in vitro. Labelled perikarya were observed in the medial and lateral habenular nuclei as well as in the posterior comm...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216557
更新日期：1987-01-01 00:00:00
abstract：:Transcriptome profiling of the pineal gland has revealed night/day differences in the expression of a major fraction of the genes active in this tissue, with two-thirds of these being nocturnal increases. A set of over 600 transcripts exhibit two-fold to >100-fold daily differences in abundance. These changes appear t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-010-1094-1
更新日期：2011-04-01 00:00:00
abstract：:Ionizing irradiation is an effective treatment for intracranial tumors but is limited by the potential adverse effects induced in surrounding normal brain. These effects can include cognitive impairments, and whereas the pathogenesis of such injury has not yet been definitively established, it may involve injury to th...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-007-0480-9
更新日期：2008-01-01 00:00:00
abstract：:The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233914
更新日期：1979-03-19 00:00:00
abstract：:Transplantation of ligament-tissue-derived stem cells has become a promising approach in the repair of injured ligament. Neovascularization plays an important role in ligament healing and remodeling. Recently, human umbilical-cord-blood-derived CD34+ cells have been reported to contribute to neoangiogenesis. Therefore...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2250-4
更新日期：2015-12-01 00:00:00
abstract：:The interstitial cells of Cajal (ICCs) are important mediators of gastrointestinal (GI) motility because of their role as pacemakers in the GI tract. In addition to their function, ICCs are also structurally distinct cells most easily identified by their ultra-structural features and expression of the tyrosine kinase ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1434-4
更新日期：2012-08-01 00:00:00
abstract：:Serine proteases, cysteine proteases, aspartic proteases and matrix metalloproteinases play an essential role in extracellular matrix remodeling and turnover through their proteolytic action on collagens, proteoglycans, fibronectin, elastin and laminin. Proteases can also act on chemokines, receptors and anti-microbia...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1390-z
更新日期：2013-02-01 00:00:00
abstract：:Osteogenic differentiation is a tightly regulated process realized by progenitor cell osteoblasts. Notch signaling pathway plays a critical role in skeletal development and bone remodeling. Controversial data exist regarding the role of Notch activation in promoting or preventing osteogenic differentiation. This study...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03130-7
更新日期：2020-01-01 00:00:00
abstract：:Bullous pemphigoid antigen-1 (BPA1) and alpha(6)beta(4)-integrin colocalize at the hemidesmosomes in basal-layer keratinocytes of normal squamous epithelia. The expression of these genes was analyzed during the process of tumor cell invasion and metastasis on frozen sections of head and neck biopsies, and the structur...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100462
更新日期：2001-12-01 00:00:00
abstract：:Mytilus mussels are characterized by annually repeated reproduction which is associated with subsequent growth, morphogenesis, breakdown and redevelopment of the gonad and reproductive tract into mantle mesenchyme. We present a description of the expression of the male-associated polypeptide (MAP; see Mikhailov et al....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051204
更新日期：1998-12-01 00:00:00
abstract：:Light microscopy of native preparations, histology, and electron microscopy have revealed that Phlebotomus duboscqi belongs to a class of sand fly species with prompt development of the peritrophic matrix (PM). Secretion of electron-lucent fibrils, presumably chitin, starts immediately after the ingestion of a blood m...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0802-1
更新日期：2009-08-01 00:00:00
abstract：:A combination of light, transmission and scanning electron microscopy was employed to demonstrate the occurrence, arrangement and structure of taste buds in the oral mucosa of the soft palate of monkeys (Macaca irus). Taste buds are found in aggregates confined to 0.15 to 0.3 mm wide, round islands of keratinizing epi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236359
更新日期：1979-01-30 00:00:00
abstract：:The amniotic epithelium constitutes a diffusion barrier controlling the passage of solutes and water between the amniotic cavity and maternal circulation. With the present immunocytochemical approach, we have shown that several major components of the cyto-skeleton, i.e., actin, alpha-actinin, spectrin and ezrin, are ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318194
更新日期：1991-11-01 00:00:00
abstract：:The distribution of nerve fibres in the mucosa of the nasal septum of the rat was investigated by means of transmission electron microscopy on transverse and tangential ultrathin sections. Near the basement membrane of respiratory and squamous epithelium, a rather dense network of unmyelinated nerve fibres occurs. Som...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318751
更新日期：1993-11-01 00:00:00
abstract：:Interstitial cells of Cajal (ICC) are morphologically and functionally intercalated between the elements of the enteric nervous system and the smooth muscle cells (SMCs) in the musculature of the digestive tract. Kit immunohistochemistry reliably identifies the location of these cells and provides information on chang...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0957-9
更新日期：2010-06-01 00:00:00
abstract：:Aromatase, the enzyme converting androgens into estrogens, is involved in many brain processes such as neural differentiation and plasticity or the prevention of cell death. We have previously observed an increase in aromatase immunoreactivity in sheep neurons exposed in vitro to the oxidant 3-nitro-L: -tyrosine. Howe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1160-3
更新日期：2011-06-01 00:00:00
abstract：:Early in postnatal life, the first wave of spermatogenesis is accompanied by an initial wave of germ cell apoptosis. This may reflect an adjustment in the number of germ cells that can be adequately maintained by Sertoli cells. Two major pathways (intrinsic and extrinsic) are involved in the process of caspase activat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0186-4
更新日期：2006-09-01 00:00:00
abstract：:The localization of inhibin alpha-subunit in the human testis was studied at the light- and electron-microscope level with immunostaining techniques. Antibodies against specific fragments of porcine and human inhibin alpha-subunits were utilized. At light microscopy, inhibin alpha-subunit immunoreactivity was detected...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00319612
更新日期：1992-08-01 00:00:00
abstract：:The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicle...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100351
更新日期：2001-04-01 00:00:00
abstract：:In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabecu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236349
更新日期：1979-01-30 00:00:00
abstract：:To identify structures involved in the translocation of the synaptic vesicles towards the presynaptic membrane, an ultrastructural study has been undertaken by means of (1) the E-PTA stain and (2) the HMM-labeling procedure. Using serial sections of E-PTA stained nervous tissue, especially those made in transversal an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219841
更新日期：1975-06-27 00:00:00
