Abstract：
:Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm to 30-nm chromatin fibers, and that the acrosomal granule forms a huge cap over the anterior pole of nucleus. In the acrosomal phase, many chromatin fibers are approximately 50 nm thick; these then thickened to 70-nm fibers and eventually became 90-nm chromatin cords that are tightly packed together into highly condensed chromatin, except where nuclear vacuoles occur. Immunocytochemistry and immunogold localization with anti-histones, anti-transition protein2, and anti-protamine antibodies suggest that histones remain throughout spermiogenesis, that transition proteins are present from step 7 spermatids and remain until the end of spermiogenesis, and that protamines appear at step 8. Spermatozoa from the cauda epididymidis have been analyzed by acid urea Triton X-100 polyacrylamide gel electrophoresis for basic nuclear proteins. The histones, H2A, H3, H2B, and H4, transitional protein2, and protamine are all present in sperm extracts. These findings suggest that, in these sperm of unusual morphology, both transition proteins and some histones are retained, a finding possibly related to the unusual nuclear form of sperm in this species.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Worawittayawong P,Leigh C,Weerachatyanukul W,Manochantr S,Sobhon P,Breed WG,Sretarugsa Pdoi
10.1007/s00441-008-0668-7subject
Has Abstractpub_date
2008-10-01 00:00:00pages
135-44issue
1eissn
0302-766Xissn
1432-0878journal_volume
334pub_type
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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journal_title：Cell and tissue research
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