A scanning electron microscopic study of the rat liver sinusoid: endothelial and Kupffer cells.


:The surface ultrastructure of Kupffer cells in the rat liver has been studied by scanning electron microscopy (SEM). The results demonstrate that Kupffer cells are both significantly different and clearly distinct from endothelial cells. Kupffer cells have neither pores (and/or "sieve plates") nor fenestrations, all of which are present in endothelial cells. They possess a stellate shape, and only indirectly, with slender and irregular evaginations, contribute to the lining of the sinusoidal wall. Furthermore, the luminal surface in some areas contains a large population of short microvilli, microphicae and invaginations. These elements form a kind of microlabyrinth which may correpond to the "worm-like" structures described by transmission electron microscopy (TEM). In the present study, transition forms between endothelial and Kupffer cells were never found. On the contrary, considering the highly fenestrated nature of the endothelial cells, the Kupffer cells may, by ameboid movements, easily cross the overlapping barrier of the sinusoid and protrude into the lumen. Thus, acting as activated macrophages, the Kupffer cells might function to prevent the entrance of foreign material into the tissues of the liver through the fragile and highly fenestrated endothelium. Finally, the topographical reconstruction of the sinusoid by correlated SEM and TEM studies demonstrates the Kupffer cells, with their protruding cytoplasm and ability to extend into the lumen of the sinusoid, may actually change the caliber of the vessel, and thus function as a "sphincter" which causes a temporary arrest of the blood flow when the diameter of the sinusoidal lumen is reduced.


Cell Tissue Res


Cell and tissue research


Motta P





Has Abstract


1975-12-10 00:00:00












  • Uptake of ferritin by the cephalopod optic gland.

    abstract::Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporti...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Froesch D,Mangold K

    更新日期:1976-08-10 00:00:00

  • Ultrastructural immunocytochemical localization of L-glutamate decarboxylase and GABA in rat pancreatic zymogen granules.

    abstract::The ultrastructural immunohistochemical localization of gamma aminobutyric acid (GABA) and its regulating enzymes, L-glutamate decarboxylase (GAD) and gamma aminobutyrate-alpha-ketoglutarate transaminase, was determined utilizing an immunogold post-embedding protocol in pancreatic exocrine tissue. Within the acinar ce...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Garry DJ,Garry MG,Sorenson RL

    更新日期:1988-04-01 00:00:00

  • Microtubular number in the tractus hypophyseus of newborn normal rats and newborn rats with congenital diabetes insipidus.

    abstract::Evidence has already been adduced suggesting that an increase in microtubular number occurs in the tractus hypophyseus of rats stressed by the administration of hypertonic saline, and of rats with congenital diabetes insipidus (CDI). Since the tractus hypophyseus in these animals shows high secretory activity, it seem...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Grainger F,Sloper JC

    更新日期:1976-06-28 00:00:00

  • Scanning electron-microscopic study of lens fibers of the pig.

    abstract::Whole pigs lenses were fixed, critical-point dried and fractured, and the internal surfaces examined with a scanning electron microscope. At various locations from the equator to the center of the lens four types of fibers can be distinguished. The superficial fibers have small interdigitations. Cortical lens fibers, ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hoyer HE

    更新日期:1982-01-01 00:00:00

  • Cellular localization of cystic fibrosis transmembrane regulator protein in piglet and mouse intestine.

    abstract::Antibodies raised against the cystic fibrosis transmembrane regulator protein (CFTR) were used to localize CFTR in intestinal tissues of piglets and mice. Positive staining for CFTR was detected in goblet cells of both species. A second population of epithelial cells of unknown phenotype was also labeled by anti-CFTR ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hayden UL,Carey HV

    更新日期:1996-02-01 00:00:00

  • Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT).

    abstract::This study aimed to characterize porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. We found that PAT is composed of a paratenon sheath, a core of fascicles, and an endotenon/interfascicular matrix (IFM) that encases the fascicle bundles. We analyzed each of th...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Zhang J,Li F,Williamson KM,Tan S,Scott D,Onishi K,Hogan MV,Wang JH

    更新日期:2021-01-26 00:00:00

  • Distribution of neurons with high-affinity uptake sites for GABA in the myenteric plexus of the guniea-pig, rat and chicken.

    abstract::The occurrence of neurons possessing high-affinity uptake sites for GABA was studied in the myenteric plexus of the guinea-pig ileum, caecum, and proximal and distal colon, the rat proximal colon, and the chicken gizzard with the use of 3H-GABA and autoradiography. Experiments were carried out on plexuses that had bee...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Saffrey MJ,Marcus N,Jessen KR,Burnstock G

    更新日期:1983-01-01 00:00:00

  • How to count cells: the advantages and disadvantages of the isotropic fractionator compared with stereology.

    abstract::The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Herculano-Houzel S,von Bartheld CS,Miller DJ,Kaas JH

    更新日期:2015-04-01 00:00:00

  • Fine structure of the pineal organ in the troglobytic fish, Typhlichthyes subterraneous (Pisces: Amblyopsidae).

    abstract::The pineal organ of the blind, cave-dwelling fish, Typhlichythyes subterraneous, was examined with both light and electron microscopes. Like the eyes, the pineal in this troglobytic species was found to be regressed. Two cell types, photoreceptor and supportive cells, were described in the pineal epithelium. Although ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: McNulty JA

    更新日期:1978-12-29 00:00:00

  • Ultrastructural and morphometric study of the lung of the European salamander, Salamandra salamandra L.

    abstract::Ultrastructural and morphometric investigations were performed on the lung of the European salamander, Salamandra salamandra L. Folds of first and second order are covered with a ciliated epithelium containing goblet cells. The respiratory surface of the lung is lined by a single type of cell which, in amphibians, com...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Goniakowska-Witalińska L

    更新日期:1978-07-27 00:00:00

  • Diapause-dependent changes in prothoracicotropic hormone-producing neurons of the tobacco hornworm, Manduca sexta.

    abstract::The prothoracicotropic hormone (PTTH), which stimulates ecdysteroid synthesis in the prothoracic glands, is produced, in the dorso-lateral protocerebrum of Manduca sexta, by paired peptidergic neurons, the lateral neurosecretory cell group III (L-NSC III). Our study revealed ultrastructural features of L-NSC III, iden...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Hartfelder K,Hanton WK,Bollenbacher WE

    更新日期:1994-07-01 00:00:00

  • Specialized contacts between sarcolemma and sarcoplasmic reticulum at the ends of muscle fibers in the diaphragm of the rat.

    abstract::An electron-microscopic study of the myotendinous portion of the diaphragm in the Wistar rat has shown that at the ends of muscle fibers, longitudinally oriented invaginations and peripheral furrows of the sarcolemma establish specialized contacts with individual sacs of the sarcoplasmic reticulum. The construction of...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Andreev DP,Wassilev WA

    更新日期:1986-01-01 00:00:00

  • Complex-type glycoproteins synthesized in the subcommissural organ of mammals. Light- and electron-microscopic investigations by use of lectins.

    abstract::The secretory activity in the subcommissural organ (SCO) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. Among the various lectins tested. Concanavalin A (Con A) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells....

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Meiniel A,Molat JL,Meiniel R

    更新日期:1988-08-01 00:00:00

  • Calbindin immunoreactivity in the enteric nervous system of larval and adult zebrafish (Danio rerio).

    abstract::Calbindin is a calcium-binding protein, commonly found in certain subpopulations of the enteric nervous system in mammals. Recently, calbindin-immunoreactive enteric neurons have also been demonstrated in shorthorn sculpin (Myoxocephalus scorpius). In the present study, calbindin immunoreactivity has been investigated...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Olsson C

    更新日期:2011-04-01 00:00:00

  • The effect of cervical vagotomy (decentralization) on the ultrastructure of the carotid body on the duck, Anas platyrhynchos.

    abstract::Carotid bodies of ducks show no detectable changes in ultrastructure up to periods of four weeks following the removal of 2-3 cm of the cervical vagus (decentralization). This indicates that the majority, and possibily all the nerves terminating on the glomus cells are afferent (sensory) in nature. These nerve endings...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Butler PJ,Osborne MP

    更新日期:1975-11-19 00:00:00

  • Cellular localisation of insulin-like growth factor binding protein-2 (IGFBP-2) during development of the marine fish, Sparus aurata.

    abstract::The spatial localisation of insulin-like growth-factor-binding protein-2 (IGFBP-2) and its mRNA was investigated during larval and post-larval developmental stages of the gilthead seabream ( Sparus aurata) by immunohistochemistry and in situ hybridisation with specific antisera and riboprobes. During larval developmen...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Radaelli G,Patruno M,Rowlerson A,Maccatrozzo L,Funkenstein B

    更新日期:2005-01-01 00:00:00

  • Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein.

    abstract::Prolonged secretory stimulation of the exocrine pancreas in the rat by in vivo infusion of caerulein leads to a rapid degranulation of the organ associated with a progressive reduction in the size of the zymogen granules. During the first six to twelve hours of stimulation Golgi complexes are enlarged and several stru...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Bieger W,Martin-Achard A,Bassler M,Kern HF

    更新日期:1976-01-28 00:00:00

  • Immunolocalization of synexin (annexin VII) in adrenal chromaffin granules and chromaffin cells: evidence for a dynamic role in the secretory process.

    abstract::Synexin (annexin VII) is a Ca(2+)- and phospholipid-binding protein which has been proposed to play a role in Ca(2+)-dependent membrane fusion processes. Using a monoclonal antibody against synexin, Mab 10E7, and immunogold, we carried out a semiquantitative localization study of synexin in bovine adrenal medullary ch...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Kuijpers GA,Lee G,Pollard HB

    更新日期:1992-08-01 00:00:00

  • Immunofluorescence of somatostatin-producing sites in the hypothalamus of the tadpole, Alytes obstetricans Laur.

    abstract::Three sites of somatostatin-synthesizing perikarya, or a related antigen, were determined by immunofluorescence in the hypothalamus of the tadpole, Alytes obstetricans (Amphibia, Anura). Two sites of neurosecretory perikarya were localized in the preoptic nuclei of the anterior hypothalamus; the axons extended either ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Rémy C,Dubois MP

    更新日期:1978-02-24 00:00:00

  • Scanning electron microscopic and x-ray diffraction studies of otoconia in the lizard Podarcis s. sicula.

    abstract::The otoliths of embryos and young animals of the lizard Podarcis s. sicula were studied by X-ray diffraction and scanning electron microscopy. Two types of crystal that give different X-ray diffraction patterns were found in the membranous labyrinth of Podarcis. The crystals consist of calcite or aragonite and are eas...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Marmo F,Franco E,Balsamo G

    更新日期:1981-01-01 00:00:00

  • Kainate receptors.

    abstract::Kainate receptors form a family of ionotropic glutamate receptors that appear to play a special role in the regulation of the activity of synaptic networks. This review first describes briefly the molecular and pharmacological properties of native and recombinant kainate receptors. It then attempts to outline the gene...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Pinheiro P,Mulle C

    更新日期:2006-11-01 00:00:00

  • Neurosecretion in the sinus gland of the fiddler crab, Uca pugnax.

    abstract::The ultrastructure of the sinus gland of the fiddler crab, Uca pugnax, was investigated and found to be similar to that in other crustaceans. Five types of neurosecretory axon terminals were tentatively identified on the basis of the size, shape, and electron density of granules within the axons. Release of neuro-secr...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Silverthorn SU

    更新日期:1975-12-29 00:00:00

  • Tissue remodelling in pulmonary fibrosis.

    abstract::Many lung diseases result in fibrotic remodelling. Fibrotic lung disorders can be divided into diseases with known and unknown aetiology. Among those with unknown aetiology, idiopathic pulmonary fibrosis (IPF) is a common diagnosis. Because of its progressive character leading to a rapid decline in lung function, it i...

    journal_title:Cell and tissue research

    pub_type: 杂志文章,评审


    authors: Knudsen L,Ruppert C,Ochs M

    更新日期:2017-03-01 00:00:00

  • Retrograde tracing and neuropeptide immunohistochemistry of sensory neurones projecting to the cartilaginous distal femoral epiphysis of young rats.

    abstract::Although cartilage is considered to be devoid of innervation, axons occur in the perichondrium and during development in cartilage canals, thereby having a relatively close spatial relationship to chondroblasts and chondrocytes. The present study locates the source of the sensory innervation of the femoral cartilagino...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Edoff K,Grenegård M,Hildebrand C

    更新日期:2000-02-01 00:00:00

  • Immunocytochemical studies on the LHRH system of the Japanese quail: influence by photoperiod and aspects of sexual differentiation.

    abstract::Immunocytochemistry was used to determine if photoperiod and/or sex have any effect on the pattern of the luteinizing hormone-releasing hormone (LHRH) system in the brain of the Japanese quail. Immunopositive perikarya were found within three major areas of the brain: the rostral paraolfactory lobe, the preoptic, and ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Foster RG,Panzica GC,Parry DM,Viglietti-Panzica C

    更新日期:1988-08-01 00:00:00

  • Ultrastructural tracer studies on the permeability of the Malpighian tubule of the pill millipede, Glomeris marginate (Villers).

    abstract::The electron-dense tracers ferritin, and iron-dextran, and the protein horseradish peroxidase, have been used to investigate the ultrastructural basis of permeability in the upper and lower segments of the Malpighian tubules of Glomeris marginata. All these materials were able to cross the basal lamina and enter the t...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Johnson IT,Riegel JA

    更新日期:1977-09-05 00:00:00

  • Postnatal expression and denervation induced up-regulation of aquaporin-5 protein in rat sweat gland.

    abstract::The evolution of aquaporin-5 (AQP5) expression during postnatal development has not been defined in the sweat gland. Previous studies have suggested that AQP isoforms in several peripheral targets are regulated by a neural mechanism. We have examined, in rat sweat glands, the expression of AQP5 during postnatal develo...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Ma L,Huang YG,He H,Deng YC,Zhang HF,Che HL,Tian JY,Zhao G

    更新日期:2007-07-01 00:00:00

  • Calcite in the statoconia of amphibians: a detailed analysis in the frog Rana esculenta.

    abstract::The otoconia of Amphibia, especially of the frog Rana esculenta, were studied by X-ray diffraction and scanning electron microscopy (SEM). The SEM studies showed that the membranous labyrinth of Amphibia contains two populations of crystals, which can easily be distinguished by their forms. The X-ray diffraction data ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Marmo F,Balsamo G,Franco E

    更新日期:1983-01-01 00:00:00

  • Expression of Slug in S100β-protein-positive cells of postnatal developing rat anterior pituitary gland.

    abstract::Among heterogeneous S100β-protein-positive (S100β-positive) cells, star-like cells with extended cytoplasmic processes, the so-called folliculo-stellate cells, envelop hormone-producing cells or interconnect homophilically in the anterior pituitary. S100β-positive cells are known, from immunohistochemistry, to emerge ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Horiguchi K,Fujiwara K,Tsukada T,Yako H,Tateno K,Hasegawa R,Takigami S,Ohsako S,Yashiro T,Kato T,Kato Y

    更新日期:2016-02-01 00:00:00

  • The midgut of Tomocerus minor Lubbock (insecta, collembola): ultrastructure, cytochemistry, ageing and renewal during a moulting cycle.

    abstract::The midgut cells of Tomocerus minor (Insecta, Collembola) were examined with the electron microscope and cytochemically. The midgut epithelium consists of a series of cells characterised by numerous mineral concretions scattered throughout the cytoplasm. Mitochondria are abundant; microvilli are well developed at the ...

    journal_title:Cell and tissue research

    pub_type: 杂志文章


    authors: Humbert W

    更新日期:1979-01-30 00:00:00