Abstract：
:Myogenesis is a complex process in which committed myogenic cells differentiate and fuse into myotubes that mature into the muscle fibres of adult organisms. This process is initiated by a cascade of myogenic regulatory factors expressed upon entry of the cells into the myogenic differentiation programme. However, external signals such as those provided by the extracellular matrix (ECM) are also important in regulating muscle differentiation and morphogenesis. In the present work, we have addressed the role of various ECM substrata on C2C12 myoblast behaviour in vitro. Cells grown on fibronectin align and fuse earlier than cells on laminin or gelatine. Live imaging of C2C12 myoblasts on fibronectin versus gelatine has revealed that fibronectin promotes a directional collective migratory behaviour favouring cell-cell alignment and fusion. We further demonstrate that this effect of fibronectin is mediated by RGD-binding integrins expressed on myoblasts, that N-cadherin contributes to this behaviour, and that it does not involve enhanced myogenic differentiation. Therefore, we suggest that the collective migration and alignment of cells seen on fibronectin leads to a more predictable movement and a positioning that facilitates subsequent fusion of myoblasts. This study highlights the importance of addressing the role of fibronectin, an abundant component of the interstitial ECM during embryogenesis and tissue repair, in the context of myogenesis and muscle regeneration.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Vaz R,Martins GG,Thorsteinsdóttir S,Rodrigues Gdoi
10.1007/s00441-012-1364-1subject
Has Abstractpub_date
2012-06-01 00:00:00pages
569-78issue
3eissn
0302-766Xissn
1432-0878journal_volume
348pub_type
杂志文章abstract：:In cattle, the oviduct plays a major role in the reproductive process; however, molecular control of oviduct receptivity to the embryo is poorly understood. A model for receptivity based on size of the pre-ovulatory follicle (POF) was used to compare oviductal morphology, cellular proliferation, and candidate transcri...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-017-2666-0
更新日期：2017-11-01 00:00:00
abstract：:Glutamate is the major excitatory neurotransmitter of the mammalian retina and glutamate uptake is essential for normal transmission at glutamatergic synapses. The reverse transcriptase-polymerase chain reaction (RT-PCR) has revealed the presence of three different high-affinity glutamate transporters in the rat retin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050976
更新日期：1998-01-01 00:00:00
abstract：:In this immunocytochemical study we investigated the distribution of nervous structures in the lower lip of adult rats. The region is characterized by a rich cutaneous and mucosal sensory innervation originating from terminal branches of the trigeminal system. Lower lip innervation was investigated by detection of the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051348
更新日期：1999-08-01 00:00:00
abstract：:The motility patterns of the reticulorumen evoke mainly mixing of the ingesta. So far unknown, intrinsic neural circuits of the enteric nervous system are involved in the control of these motility patterns. The aim of the study was to characterize neurochemically sheep ruminal myenteric neurones, in particular the neu...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0554-7
更新日期：2002-09-01 00:00:00
abstract：:The effects of the administration of thyroxine (T4) on the postnatal cytodifferentiation of granular convoluted tubule (GCT) cells of the submandibular gland (SMG) of Lewiss-Webster mice were studied by light and electron microscopy. From birth, mice of both sexes were injected daily with T4 (sc 0.4 micrograms/g BW) a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218202
更新日期：1987-05-01 00:00:00
abstract：:Exposure to nicotine in smoking contributes to most unexplained male infertility but the mechanisms remain to be fully elucidated. Zinc (Zn) is an essential trace element in normal growth, development and reproduction. Zinc oxide nanoparticles (ZnONPs) are well-known antioxidants. Therefore, this work was designed to ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2909-8
更新日期：2019-02-01 00:00:00
abstract：:The capsule of the muscle spindle from the anterior and posterior latissimus dorsi muscles of the adult domestic chicken has been studied with the electron microscope. As in other species, two distinct portions of the spindle capsule are distinguished: an outer capsule and an inner capsule. The outer capsule is struct...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220126
更新日期：1976-02-25 00:00:00
abstract：:The nine-banded armadillo possesses a salivary bladder which is a dilated portion of the main duct of the submandibular gland at its origin. The wall of the bladder is composed of an epithelium, a submucosa and a thick coat of skeletal muscle. The ultrastructure of the epithelium reveals that it is complex and consist...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219609
更新日期：1976-06-28 00:00:00
abstract：:The dorsal root ganglion (DRG) and dorsal horn of the spinal cord are areas through which primary afferent information passes enroute to the brain. Previous studies have reported that, during normal neuronal activity, the regional distribution of a second messenger, diacylglycerol (DG), which is derived from phosphoin...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-006-0219-z
更新日期：2006-10-01 00:00:00
abstract：:Using a somatostatin-gold conjugate of known biological activity, high affinity binding sites for this neuropeptide were visualized at cellular resolution on cultured diencephalic astrocytes and on frozen sections of the rat diencephalon. Binding could be completely suppressed in competition experiments with surplus u...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318767
更新日期：1991-02-01 00:00:00
abstract：:The blood-brain barrier (BBB) protects the brain from toxic substances within the bloodstream and keeps the brain's homeostasis stable. On the other hand, it also represents the main obstacle in the treatment of many CNS diseases. Among different techniques, nanoparticles have emerged as promising tools to enhance bra...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-014-1819-7
更新日期：2014-03-01 00:00:00
abstract：:Striated rootlets in ciliated cells are conical banded structures composed of longitudinally aligned filaments. The formation of striated rootlets during ciliognesis in the human oviduct epithelium was studied by electron microscopy. Primitive rootlets appeared at the proximal side of basal bodies before or at the sam...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050905
更新日期：1997-10-01 00:00:00
abstract：:Interstitial cells in the myenteric plexus and the deep muscular plexus of the small intestine of the c-kit mutant rats (Ws/Ws) and their normal siblings (+/+) were studied. c-Kit immunoreactivity was detected in two regions corresponding to the myenteric plexus and the deep muscular plexus in the jejunum of +/+ rats,...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051119
更新日期：1998-08-01 00:00:00
abstract：:Daily fluctuations of nucleoli and nucleolar fibrillar centres in neurosecretory cells from the supraoptic nucleus (SON) were investigated in rats artificially synchronized for 3 weeks to a set 12 h light/12 h dark cycle with free access to food and water. Group of 3 animals were sacrificed by intracardiac perfusion e...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222351
更新日期：1985-01-01 00:00:00
abstract：:Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
abstract：:This study documents neuronal differentiation in vitro from undifferentiated precursor cells of caudalmost regenerating spinal cord of the teleost Apteronotus albifrons. At 11 days in vitro, cells from the caudalmost tip of the regenerating cord are flat and polygonal in shape, lack neuronal processes and do not stain...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00414166
更新日期：1994-11-01 00:00:00
abstract：:The endoneurial collagen sheath around teased nerve fibres following crush injury was studied by scanning electron microscopy and compared with uninjured sciatic nerve fibres and with fibres from the dystonic mutant mouse. Following crush injury the endoneurial collagen became more abundant than seen in untreated nerv...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00210469
更新日期：1981-01-01 00:00:00
abstract：:The distributions within the coeliac ganglion of different chemically coded subgroups of noradrenaline neurons, and the relationships between these neurons and nerve fibres projecting to the ganglion from the intestine, have been assessed quantitatively by use of an immunohistochemical double-staining method. Noradren...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218395
更新日期：1986-01-01 00:00:00
abstract：:Low-temperature-embedded tissue of the subcommissural organ (SCO) of the rabbit was analyzed for the basal route of secretory product by means of indirect immuno-metal cytochemistry (protein A-gold technique) at the electron-microscopic level. By use of (1) an antiserum against bovine Reissner's fibre (see Sterba et a...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219221
更新日期：1986-01-01 00:00:00
abstract：:In vitro incubation of autonomic nerves with vinblastine sulphate (1 times 10(-4) M) caused a disappearance of microtubules within 15 min; during the following 15 min paracrystalline arrays appeared within the axons. An increase in the abundance of microfilaments was also observed, but these did not appear to arise fr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220136
更新日期：1976-02-25 00:00:00
abstract：:The immunocytochemical localization of bovine oviduct-specific glycoproteins was investigated by light and electron microscopy. Using monoclonal antibodies (MAbs) specific for bovine oviductal glycoproteins, 3 regions (fimbriae, ampulla, and isthmus) of the epithelium in the bovine oviduct we studied during the follic...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327983
更新日期：1993-10-01 00:00:00
abstract：:The distribution and abundance of nitric oxide synthase (NOS)-containing neurons and their terminals in the gastrointestinal tract of the guinea-pig were examined in detail using NADPH diaphorase histochemistry and NOS immunohistochemistry. NOS-containing cell bodies were found in the myenteric plexus throughout the g...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00303090
更新日期：1994-07-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217869
更新日期：1984-01-01 00:00:00
abstract：:The immunohistochemical distribution of the basement membrane (BM) proteins, laminin and type IV collagen, and interstitial type III collagen was investigated in 12 fetal spleens at the 15th-38th gestational weeks (g.w.) and in spleens of 8 infants from term to 4 years. The results were compared with the distribution ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318766
更新日期：1991-02-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00231023
更新日期：1978-08-25 00:00:00
abstract：:The effect of phenylthiourea (PTU) on the pigment epithelium and the photoreceptor cells in the developing retina of Haplochromis burtoni was studied by electron microscopy. In the retinal pigment epithelium of 6-day old embryos, both types of melanin granule (spindle-shaped and rod-shaped) are already found. PTU inhi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219054
更新日期：1977-03-09 00:00:00
abstract：:In recent years, data have accumulated suggesting that the role of neurotrophins and Trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. This study was designed to map the expression of immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410000181
更新日期：2000-04-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00223015
更新日期：1975-12-10 00:00:00
abstract：:The distribution of estradiol-concentrating cells in the pituitary of the platyfish, Xiphophorus maculatus, is studied after the injection of 3H estradiol-17 beta by thaw-mount autoradiography. Autoradiograms prepared 2-8 h after the injection show nuclear concentration of radioactivity in certain cells of the rostral...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00234188
更新日期：1979-05-25 00:00:00
abstract：:Although the pharmacological properties and distribution of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores vary considerably among tissues, studies of its localization have been devoted mostly to the central nervous system. In this report we have analyzed the localization of IP3 receptors in diverse non-n...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050641
更新日期：1996-08-01 00:00:00
